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The map label for this gene is nylB [H]

Identifier: 86748619

GI number: 86748619

Start: 1687037

End: 1688554

Strand: Reverse

Name: nylB [H]

Synonym: RPB_1494

Alternate gene names: 86748619

Gene position: 1688554-1687037 (Counterclockwise)

Preceding gene: 86748620

Following gene: 86748617

Centisome position: 31.67

GC content: 66.01

Gene sequence:

>1518_bases
ATGAAGCTTCGGGAACTCGCGCATTCGCGCACCGGCGACAAAGGCAACAGGTCGAACATCTCCCTGATTCCCTACGATGC
GTCGCACTATCAGCACCTGCGCGACATCGTCACCGCCGAACGCGTGAAGGAACATCTGCGCCACATCGTGTACGGCGCCG
TCGAACGCTACGACTTGCCGAATGTCGGTGCCTTCAACTTCGTGCTCGACGACGCGCTGGGCGGCGGCGTCGCCAGATCG
CTGTCGCTGGACAGCCACGGCAAGTCGCTGAGTTCGGCGCTGCTGGAGATGGACGTGCCCGCGCCGCCCGGCTACGCGGC
GACGGCGCGGAACGGGTCGGGCACTCCGGCGACGCCGACCGCGCATCTGCCCAGGGCCGAGGATTTTCTGCTGTGGCCGC
CGAACGTCCAGGCCTACGGCTATCGGATCGTCGACAAGCTGTTCAACTCCCGCGTCATCCGCAAGGGCCCCTCGCCGCGC
CCACTCCGCTATGGGCAGCACGTCCAGATCGAGTACCGAAGCGACGGAGCGACGTTGTCGCTCGACGATTTCATCGATCG
GAATTCGCTGAGCGGCCTCATCGTCCTGAAGAACGGAAAGGTCGTGATCGAGCAGTACGGGCTGGGGCTGTTGCCATCCG
ACCGCTGGAGCACGATGTCGACCGTCAAATCGATCACCGCGACCCTGGTGGGCGTCGCGCTGCAGGACGGCGCGATCCAT
TCGCTCGACGACGCGGTGACGAATTACGTGCCGTCGCTCGCAGGCTCCGCCTACGAAGACGTCGCCGTCAAGCACCTGCT
GACGATGTCGTCCGGCATCCGATGGAACGAAGACTATACCGACAAGCAATCGGACGTGAACCGCTACAGCAAATCTCTCG
CCGACGGCGTGCCCGGTGGCGTCTTTGCTCTGCTGAAGGCGCTGCCGCGTGAGCACGCAGCCGGCGCGCACTGGCACTAC
AACACGGGCGACACCTTCCTGCTCGGCGCGGTGTTGCGCAGCGCCGTCGGCATGCCGCTCGCCGACTACATGTCGCGCAA
GATCTGGCAGCCCTGCGGCATGGAATTCGACGCGTTCTATACGCTGGAGTCGCCGGACGGACTGGAGATCGGCGGCAGCC
GCGCAGGCATCGCGCTGCGCGATTTCGGCCGGTTCGCGCAATTCGTGCTCGACGACGGCGTCGTGGACGGCGCGCGCCTG
CTGCCGGCGGGATGGAACGACGACGCCAACCGCCGGGCCTATAAGTTCTCCGATGCCGACCGGGCCCTGATGCCCCAGAT
CAGTTCGGGGGCGCTGGACGGCTACGGTTACAGTTGGTGGATCGCCGAGGACGACGCGATGACGGCGGTGGGCTTCGCCG
GGCAGCGGATCTACATCAACCGCGCCGAGCAGCTCGCCATCGTCACGCTCGGCGCATTTCCGCAGCCGAGATACCGCGGC
CCCGGCGAGCACGACAGGGTGGCGGAGGTCGTCGCCTTCACCCAAGCGGTGAAATCGGCGCTGCAGGACGACGACTAG

Upstream 100 bases:

>100_bases
CGGCGGCGTGGTTCAACTGGTCCGCGAGGTAATCGCGGTGCAATCGGTGCTGATCCCGCGCGACCAGACGAGTCCGGTCG
TCACCATGTTGGAGGCGTAG

Downstream 100 bases:

>100_bases
TCCAGTCGCGCACGCCCTCGCTGCGGAACATCAGGGTCCGCAGTGAGGGCACGGCGCGCTACCCGCCGCTCCGGATCAGC
TTGCTCACAGTCCGGGCGAA

Product: Beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKLRELAHSRTGDKGNRSNISLIPYDASHYQHLRDIVTAERVKEHLRHIVYGAVERYDLPNVGAFNFVLDDALGGGVARS
LSLDSHGKSLSSALLEMDVPAPPGYAATARNGSGTPATPTAHLPRAEDFLLWPPNVQAYGYRIVDKLFNSRVIRKGPSPR
PLRYGQHVQIEYRSDGATLSLDDFIDRNSLSGLIVLKNGKVVIEQYGLGLLPSDRWSTMSTVKSITATLVGVALQDGAIH
SLDDAVTNYVPSLAGSAYEDVAVKHLLTMSSGIRWNEDYTDKQSDVNRYSKSLADGVPGGVFALLKALPREHAAGAHWHY
NTGDTFLLGAVLRSAVGMPLADYMSRKIWQPCGMEFDAFYTLESPDGLEIGGSRAGIALRDFGRFAQFVLDDGVVDGARL
LPAGWNDDANRRAYKFSDADRALMPQISSGALDGYGYSWWIAEDDAMTAVGFAGQRIYINRAEQLAIVTLGAFPQPRYRG
PGEHDRVAEVVAFTQAVKSALQDDD

Sequences:

>Translated_505_residues
MKLRELAHSRTGDKGNRSNISLIPYDASHYQHLRDIVTAERVKEHLRHIVYGAVERYDLPNVGAFNFVLDDALGGGVARS
LSLDSHGKSLSSALLEMDVPAPPGYAATARNGSGTPATPTAHLPRAEDFLLWPPNVQAYGYRIVDKLFNSRVIRKGPSPR
PLRYGQHVQIEYRSDGATLSLDDFIDRNSLSGLIVLKNGKVVIEQYGLGLLPSDRWSTMSTVKSITATLVGVALQDGAIH
SLDDAVTNYVPSLAGSAYEDVAVKHLLTMSSGIRWNEDYTDKQSDVNRYSKSLADGVPGGVFALLKALPREHAAGAHWHY
NTGDTFLLGAVLRSAVGMPLADYMSRKIWQPCGMEFDAFYTLESPDGLEIGGSRAGIALRDFGRFAQFVLDDGVVDGARL
LPAGWNDDANRRAYKFSDADRALMPQISSGALDGYGYSWWIAEDDAMTAVGFAGQRIYINRAEQLAIVTLGAFPQPRYRG
PGEHDRVAEVVAFTQAVKSALQDDD
>Mature_505_residues
MKLRELAHSRTGDKGNRSNISLIPYDASHYQHLRDIVTAERVKEHLRHIVYGAVERYDLPNVGAFNFVLDDALGGGVARS
LSLDSHGKSLSSALLEMDVPAPPGYAATARNGSGTPATPTAHLPRAEDFLLWPPNVQAYGYRIVDKLFNSRVIRKGPSPR
PLRYGQHVQIEYRSDGATLSLDDFIDRNSLSGLIVLKNGKVVIEQYGLGLLPSDRWSTMSTVKSITATLVGVALQDGAIH
SLDDAVTNYVPSLAGSAYEDVAVKHLLTMSSGIRWNEDYTDKQSDVNRYSKSLADGVPGGVFALLKALPREHAAGAHWHY
NTGDTFLLGAVLRSAVGMPLADYMSRKIWQPCGMEFDAFYTLESPDGLEIGGSRAGIALRDFGRFAQFVLDDGVVDGARL
LPAGWNDDANRRAYKFSDADRALMPQISSGALDGYGYSWWIAEDDAMTAVGFAGQRIYINRAEQLAIVTLGAFPQPRYRG
PGEHDRVAEVVAFTQAVKSALQDDD

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 55146; Mature: 55146

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRELAHSRTGDKGNRSNISLIPYDASHYQHLRDIVTAERVKEHLRHIVYGAVERYDLP
CCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVGAFNFVLDDALGGGVARSLSLDSHGKSLSSALLEMDVPAPPGYAATARNGSGTPATPT
CCCEEHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCC
AHLPRAEDFLLWPPNVQAYGYRIVDKLFNSRVIRKGPSPRPLRYGQHVQIEYRSDGATLS
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEE
LDDFIDRNSLSGLIVLKNGKVVIEQYGLGLLPSDRWSTMSTVKSITATLVGVALQDGAIH
HHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
SLDDAVTNYVPSLAGSAYEDVAVKHLLTMSSGIRWNEDYTDKQSDVNRYSKSLADGVPGG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
VFALLKALPREHAAGAHWHYNTGDTFLLGAVLRSAVGMPLADYMSRKIWQPCGMEFDAFY
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEE
TLESPDGLEIGGSRAGIALRDFGRFAQFVLDDGVVDGARLLPAGWNDDANRRAYKFSDAD
EECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEECCCC
RALMPQISSGALDGYGYSWWIAEDDAMTAVGFAGQRIYINRAEQLAIVTLGAFPQPRYRG
HHHCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCCC
PGEHDRVAEVVAFTQAVKSALQDDD
CCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLRELAHSRTGDKGNRSNISLIPYDASHYQHLRDIVTAERVKEHLRHIVYGAVERYDLP
CCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVGAFNFVLDDALGGGVARSLSLDSHGKSLSSALLEMDVPAPPGYAATARNGSGTPATPT
CCCEEHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCC
AHLPRAEDFLLWPPNVQAYGYRIVDKLFNSRVIRKGPSPRPLRYGQHVQIEYRSDGATLS
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEE
LDDFIDRNSLSGLIVLKNGKVVIEQYGLGLLPSDRWSTMSTVKSITATLVGVALQDGAIH
HHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
SLDDAVTNYVPSLAGSAYEDVAVKHLLTMSSGIRWNEDYTDKQSDVNRYSKSLADGVPGG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
VFALLKALPREHAAGAHWHYNTGDTFLLGAVLRSAVGMPLADYMSRKIWQPCGMEFDAFY
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEE
TLESPDGLEIGGSRAGIALRDFGRFAQFVLDDGVVDGARLLPAGWNDDANRRAYKFSDAD
EECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEECCCC
RALMPQISSGALDGYGYSWWIAEDDAMTAVGFAGQRIYINRAEQLAIVTLGAFPQPRYRG
HHHCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCCC
PGEHDRVAEVVAFTQAVKSALQDDD
CCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]