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The map label for this gene is nrtB [H]

Identifier: 86748598

GI number: 86748598

Start: 1662280

End: 1663200

Strand: Reverse

Name: nrtB [H]

Synonym: RPB_1473

Alternate gene names: 86748598

Gene position: 1663200-1662280 (Counterclockwise)

Preceding gene: 86748599

Following gene: 86748597

Centisome position: 31.19

GC content: 65.69

Gene sequence:

>921_bases
ATGGAAAGCTCCGCGGTGCGGCGCGACCAGCAGGGGCCGGACTCGCCGGCCGCTGCCTTCCGTGTGGGCTGGCTCCCGTC
GGCCGCCACGGCCCCGACCGCGCCGCGCCGCGGCGCGGCTTCGCCGGGGACGTATATCAGGCGCTGGCTCAAGCTCAACC
GCGGCGGCCTGCGCGCATTGCTGATCGGCGCGGTCGCGCTGGTGCTGTTCCTGTTCGCCTGGCACCTGCTCACCGCCAAT
CGCGTGGTGTTCTTCGTCCGCTTCACCAACGTGCCCTCGCCCGCCGCGGTCTACGACAGCCTGACCCGAGCGATGCACGA
TTCGAAGTTCTTCATGCACGTCCTGCTGAGCTGCCGCCGCATCCTGATCGGCTTCTCGCTGGCCGCATTGATCGCGGTGC
CGCTCGGGATGGTGATGGGCCGCTTCAAGCTGATCCACGAGGCGCTGTTTCCGATCAGCGAGGTGCTGCGGCCGATCCCG
GCGATCGCCTGGGTGCCGATGGCGATCATGTTGTGGCCGACCAACGAGCAGAGCATTGTCTTCATCACCTTCCTCGGCTC
GTTCTTTCCGATCCTGGTCAACACTCTGCACGGCATGAAACTGGTCGATCCCGTGCTGGTGCGCGCCTCGCAATGCCTCG
GCGCCCGCGAATTTGCGATCTTCCGAGAAGTGTATTTTCCCGCCTCGCTGCCGCACATCTTCACCGGCCTCACCGTCGGC
ATGGGCGTGGCCTGGGTGTCGCTGATCGCCGCCGAGATGATCTCCGGCCAGTTCGGCATCGGCTATTTCACCTGGGAAGC
CTATTCACTCGTGCAGTATGCCGACATCGCGCTGGGGATGATCTGCATCGGCGTGCTGGGGCTCGGCTCCAGCATGTTGA
TCCGCGGGCTGGGCCGCCTCGTCATGCCATGGAGCAAATAA

Upstream 100 bases:

>100_bases
TCCTGCTCAAGAAGGACGCCGAAGCGCATGCCGCCAAGATCAACGGCAAGGTGCTGAATTTCGACGAAGCCCTCAAGTCA
GCGGTCAGCGGAGGTTGACG

Downstream 100 bases:

>100_bases
TGAGCACGCAAGCGCCGAAACCGACACCGACCAAGGGCCGCGTCGACGTCCGTAATTTCGGGCTGAGCTATGAGACGCTC
GACGGGCCGATGGAGGCGGT

Product: binding-protein dependent transport system inner membrane protein

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MESSAVRRDQQGPDSPAAAFRVGWLPSAATAPTAPRRGAASPGTYIRRWLKLNRGGLRALLIGAVALVLFLFAWHLLTAN
RVVFFVRFTNVPSPAAVYDSLTRAMHDSKFFMHVLLSCRRILIGFSLAALIAVPLGMVMGRFKLIHEALFPISEVLRPIP
AIAWVPMAIMLWPTNEQSIVFITFLGSFFPILVNTLHGMKLVDPVLVRASQCLGAREFAIFREVYFPASLPHIFTGLTVG
MGVAWVSLIAAEMISGQFGIGYFTWEAYSLVQYADIALGMICIGVLGLGSSMLIRGLGRLVMPWSK

Sequences:

>Translated_306_residues
MESSAVRRDQQGPDSPAAAFRVGWLPSAATAPTAPRRGAASPGTYIRRWLKLNRGGLRALLIGAVALVLFLFAWHLLTAN
RVVFFVRFTNVPSPAAVYDSLTRAMHDSKFFMHVLLSCRRILIGFSLAALIAVPLGMVMGRFKLIHEALFPISEVLRPIP
AIAWVPMAIMLWPTNEQSIVFITFLGSFFPILVNTLHGMKLVDPVLVRASQCLGAREFAIFREVYFPASLPHIFTGLTVG
MGVAWVSLIAAEMISGQFGIGYFTWEAYSLVQYADIALGMICIGVLGLGSSMLIRGLGRLVMPWSK
>Mature_306_residues
MESSAVRRDQQGPDSPAAAFRVGWLPSAATAPTAPRRGAASPGTYIRRWLKLNRGGLRALLIGAVALVLFLFAWHLLTAN
RVVFFVRFTNVPSPAAVYDSLTRAMHDSKFFMHVLLSCRRILIGFSLAALIAVPLGMVMGRFKLIHEALFPISEVLRPIP
AIAWVPMAIMLWPTNEQSIVFITFLGSFFPILVNTLHGMKLVDPVLVRASQCLGAREFAIFREVYFPASLPHIFTGLTVG
MGVAWVSLIAAEMISGQFGIGYFTWEAYSLVQYADIALGMICIGVLGLGSSMLIRGLGRLVMPWSK

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=219, Percent_Identity=30.5936073059361, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87081802, Length=215, Percent_Identity=30.2325581395349, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33567; Mature: 33567

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSAVRRDQQGPDSPAAAFRVGWLPSAATAPTAPRRGAASPGTYIRRWLKLNRGGLRAL
CCCCHHCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
LIGAVALVLFLFAWHLLTANRVVFFVRFTNVPSPAAVYDSLTRAMHDSKFFMHVLLSCRR
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGFSLAALIAVPLGMVMGRFKLIHEALFPISEVLRPIPAIAWVPMAIMLWPTNEQSIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEE
FITFLGSFFPILVNTLHGMKLVDPVLVRASQCLGAREFAIFREVYFPASLPHIFTGLTVG
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
MGVAWVSLIAAEMISGQFGIGYFTWEAYSLVQYADIALGMICIGVLGLGSSMLIRGLGRL
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VMPWSK
CCCCCC
>Mature Secondary Structure
MESSAVRRDQQGPDSPAAAFRVGWLPSAATAPTAPRRGAASPGTYIRRWLKLNRGGLRAL
CCCCHHCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
LIGAVALVLFLFAWHLLTANRVVFFVRFTNVPSPAAVYDSLTRAMHDSKFFMHVLLSCRR
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGFSLAALIAVPLGMVMGRFKLIHEALFPISEVLRPIPAIAWVPMAIMLWPTNEQSIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEE
FITFLGSFFPILVNTLHGMKLVDPVLVRASQCLGAREFAIFREVYFPASLPHIFTGLTVG
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
MGVAWVSLIAAEMISGQFGIGYFTWEAYSLVQYADIALGMICIGVLGLGSSMLIRGLGRL
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VMPWSK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]