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The map label for this gene is lpsJ [H]

Identifier: 86748584

GI number: 86748584

Start: 1648714

End: 1649364

Strand: Direct

Name: lpsJ [H]

Synonym: RPB_1459

Alternate gene names: 86748584

Gene position: 1648714-1649364 (Clockwise)

Preceding gene: 86748583

Following gene: 86748586

Centisome position: 30.92

GC content: 64.52

Gene sequence:

>651_bases
ATGGCCTGGACCCGCGAACAGATGGCAGCGCGCGCCGCCAAGGAATTGCGCGACGGTTTCTACGTCAATCTCGGTATCGG
GATTCCGACGCTGGTGTCGAACTACATCCCCGACGGCGTCGACGTCGTGCTGCAGAGCGAGAACGGCATGCTCGGCATGG
GCCCGTTTCCCTACGAGGGCGAGGAAGATCCGGACCTGATCAACGCCGGCAAGCAGACCGTCACCGAGCTGCCGTCGACC
AGCTACTTCTCGTCCGCCGACTCGTTCGCGATGGTGCGCGGCGGCCACATCGACCTGTCGATCCTCGGCGCCATGCAGGT
CGCCCAGAACGGCGATCTCGCCAACTGGATGATCCCCGGCAAGATGGTCAAGGGCATGGGCGGCGCGATGGACCTCGTCG
CCGGCGTCAAGCGCGTCGTGGTGGTAATGGAGCATTCCGCCAAGGACGGCCCGAAGCTGCTGAAGTCTTGCAACCTGCCG
CTGACCGGCGAGCGCGTCGTCGACATGGTGGTGACCGATCTGGCGGTGTTCACCATCGACAAGCACGGCAACGATGGCAT
GGCGCTGATCGAACTCGCCGACGGCGTCACGCTCGACCAGGTCAAGGCCAACACCGAAGCCGAATTCCGCGTCGCGCTGA
AGAACGCGTAG

Upstream 100 bases:

>100_bases
TCGTCCAGCGCATCATCGAAGTCGGCTCCGCCAAGAAGCACATCGAGCAGCGCACCACCCGCAAGCGCCCCGAAGCCGCG
GCAACCTGAGGAGAGTTCTG

Downstream 100 bases:

>100_bases
CTCGCTGTGTAGGGTGGGCAAAGGCGCGCAACGCCGTGCCCACGCGACCGCCGAGGTTCGACCGACGCGGGCTTCGCTTC
ACTCAGCACCACCCTGCGAG

Product: 3-oxoacid CoA-transferase, subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MAWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPDGVDVVLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPST
SYFSSADSFAMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKSCNLP
LTGERVVDMVVTDLAVFTIDKHGNDGMALIELADGVTLDQVKANTEAEFRVALKNA

Sequences:

>Translated_216_residues
MAWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPDGVDVVLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPST
SYFSSADSFAMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKSCNLP
LTGERVVDMVVTDLAVFTIDKHGNDGMALIELADGVTLDQVKANTEAEFRVALKNA
>Mature_215_residues
AWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPDGVDVVLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPSTS
YFSSADSFAMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKSCNLPL
TGERVVDMVVTDLAVFTIDKHGNDGMALIELADGVTLDQVKANTEAEFRVALKNA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=212, Percent_Identity=59.4339622641509, Blast_Score=255, Evalue=2e-68,
Organism=Homo sapiens, GI11545841, Length=210, Percent_Identity=58.5714285714286, Blast_Score=244, Evalue=6e-65,
Organism=Escherichia coli, GI1788552, Length=209, Percent_Identity=53.5885167464115, Blast_Score=228, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=56.3981042654028, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21358325, Length=210, Percent_Identity=60.952380952381, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655794, Length=210, Percent_Identity=60.952380952381, Blast_Score=268, Evalue=2e-72,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23073; Mature: 22942

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPDGVDVVLQSENGMLGMGPFPYEG
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTVTELPSTSYFSSADSFAMVRGGHIDLSILGAMQVAQNGDLANWMIPG
CCCCHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCEEECCH
KMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKSCNLPLTGERVVDMVVTDLAVFTID
HHHHCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEEEEE
KHGNDGMALIELADGVTLDQVKANTEAEFRVALKNA
CCCCCCEEEEEECCCCEEHHHCCCCCCEEEEEECCC
>Mature Secondary Structure 
AWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPDGVDVVLQSENGMLGMGPFPYEG
CCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTVTELPSTSYFSSADSFAMVRGGHIDLSILGAMQVAQNGDLANWMIPG
CCCCHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCEEECCH
KMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKSCNLPLTGERVVDMVVTDLAVFTID
HHHHCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEEEEE
KHGNDGMALIELADGVTLDQVKANTEAEFRVALKNA
CCCCCCEEEEEECCCCEEHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]