BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is yngF [H]

Identifier: 86748429

GI number: 86748429

Start: 1485956

End: 1486771

Strand: Reverse

Name: yngF [H]

Synonym: RPB_1304

Alternate gene names: 86748429

Gene position: 1486771-1485956 (Counterclockwise)

Preceding gene: 86748430

Following gene: 86748428

Centisome position: 27.89

GC content: 67.03

Gene sequence:

>816_bases
ATGACCGACACGCAACGCGCCGTACAGACTGCTTCGAGCGAAGTGCTCTATGCCGTCGAAGACCACATCGCCACCATCAC
ACTGAACGCGCCGGAGCGGATGAACACCATCTCCGGGCCGATGCTGAACGAGCTGGCGGCGCTGCTGCTCAGGGCCAATG
AAGACGCCGACGTGCGCTGCGTGATCCTCACCGGCAATGGCCGCGCGTTCTGCGCCGGGCTCGATTTGCGCAAGGAGCGC
GGCGACGAAGGGCTCAGCGCGGCGTCGTCGCCGACCACGCTGGATCTGCGCAGCACCCCGCCGACCGTGCTGCAGGCGAT
GGACAAGCCGACGATCTGCGCGGTGAACGGCGGCGCCGCCGGCTACGGCATGGACACCGCGCTCGGCTGCGACATCCGCA
TCATGGCGGAATCCGCCAAGCTCGCCGCGGCCTTCGTCAAGCGCGGCGTGGTGCCGGAATCCGGCGGCACCTGGTTTCTG
CCGCGGATGATCGGCTGGGCGAAGGCCGCCGAGTTGATCTTCACCGGCCGCACGCTGAGCGCGCGCGAGAGTCTCGACTG
GGGGCTCGCCAACGAAGTGGTGCCCGATTCCGAGCTGATGGGCCGCGCCCGCGCGGTGGCGAAGGAGATCGCCGGCAACG
CGCCGCTGGCGGTGCAGGCCGCCAAGCGAATGATGCGGATCGGCCTCAACGAGACCTTCCCCGATCACGTCCATCACGTC
TATCTGCAACTGCTGCCGCTGTTCAAGACGCAGGACATGGCCGAAGGCATCAAGGCCTTCATGGAAAAACGCGAGCCGAA
ATTCGTCGGTCGCTGA

Upstream 100 bases:

>100_bases
GCACGGCGCCCCTGGATTGCTTCGTCGGCTTCGCCTCCTCGCAATGACGAGGAGGCGACGAACGACAACATGACAAACAA
CAACAATCAGGAGGAAACCG

Downstream 100 bases:

>100_bases
TACGCGGCAGCGGAGGCGCCACCATGTTCGAGACCGTCACCACGATACTCGACTGGATCGGCGTCGTCGCGTTCACCGTC
TCGGGCGCGTTGGTGGCGTC

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTDTQRAVQTASSEVLYAVEDHIATITLNAPERMNTISGPMLNELAALLLRANEDADVRCVILTGNGRAFCAGLDLRKER
GDEGLSAASSPTTLDLRSTPPTVLQAMDKPTICAVNGGAAGYGMDTALGCDIRIMAESAKLAAAFVKRGVVPESGGTWFL
PRMIGWAKAAELIFTGRTLSARESLDWGLANEVVPDSELMGRARAVAKEIAGNAPLAVQAAKRMMRIGLNETFPDHVHHV
YLQLLPLFKTQDMAEGIKAFMEKREPKFVGR

Sequences:

>Translated_271_residues
MTDTQRAVQTASSEVLYAVEDHIATITLNAPERMNTISGPMLNELAALLLRANEDADVRCVILTGNGRAFCAGLDLRKER
GDEGLSAASSPTTLDLRSTPPTVLQAMDKPTICAVNGGAAGYGMDTALGCDIRIMAESAKLAAAFVKRGVVPESGGTWFL
PRMIGWAKAAELIFTGRTLSARESLDWGLANEVVPDSELMGRARAVAKEIAGNAPLAVQAAKRMMRIGLNETFPDHVHHV
YLQLLPLFKTQDMAEGIKAFMEKREPKFVGR
>Mature_270_residues
TDTQRAVQTASSEVLYAVEDHIATITLNAPERMNTISGPMLNELAALLLRANEDADVRCVILTGNGRAFCAGLDLRKERG
DEGLSAASSPTTLDLRSTPPTVLQAMDKPTICAVNGGAAGYGMDTALGCDIRIMAESAKLAAAFVKRGVVPESGGTWFLP
RMIGWAKAAELIFTGRTLSARESLDWGLANEVVPDSELMGRARAVAKEIAGNAPLAVQAAKRMMRIGLNETFPDHVHHVY
LQLLPLFKTQDMAEGIKAFMEKREPKFVGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=30.1526717557252, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=31.4516129032258, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI150378541, Length=261, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=32.03125, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=30.4761904761905, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=30.4761904761905, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=210, Percent_Identity=30.4761904761905, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=32.7683615819209, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538581, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI25453481, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI51538573, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221136756, Length=203, Percent_Identity=28.0788177339901, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI221136753, Length=203, Percent_Identity=28.0788177339901, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=28.2178217821782, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=221, Percent_Identity=25.7918552036199, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI49355787, Length=221, Percent_Identity=25.7918552036199, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=26.3440860215054, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=28.042328042328, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=28.042328042328, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116235487, Length=237, Percent_Identity=24.0506329113924, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI213417737, Length=217, Percent_Identity=24.8847926267281, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI157694516, Length=217, Percent_Identity=24.8847926267281, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=38.3141762452107, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.2046332046332, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=34.9624060150376, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=30.5019305019305, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788682, Length=210, Percent_Identity=31.9047619047619, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=29.0816326530612, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=30.9236947791165, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=32.6612903225806, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=29.7959183673469, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=27.2373540856031, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=216, Percent_Identity=29.6296296296296, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=25.7936507936508, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=287, Percent_Identity=23.6933797909408, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=265, Percent_Identity=26.0377358490566, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=29.2307692307692, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=29.2307692307692, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=176, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653139, Length=267, Percent_Identity=33.7078651685393, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=29.9625468164794, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=28.6274509803922, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=25.4901960784314, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=24.3027888446215, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24650670, Length=264, Percent_Identity=30.6818181818182, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 29183; Mature: 29052

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQRAVQTASSEVLYAVEDHIATITLNAPERMNTISGPMLNELAALLLRANEDADVRC
CCCHHHHHHHHCCCEEEEEECCEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEE
VILTGNGRAFCAGLDLRKERGDEGLSAASSPTTLDLRSTPPTVLQAMDKPTICAVNGGAA
EEEECCCEEEEECCCHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCEEEECCCCC
GYGMDTALGCDIRIMAESAKLAAAFVKRGVVPESGGTWFLPRMIGWAKAAELIFTGRTLS
CCCCCHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHEEECCCCC
ARESLDWGLANEVVPDSELMGRARAVAKEIAGNAPLAVQAAKRMMRIGLNETFPDHVHHV
HHHHCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
YLQLLPLFKTQDMAEGIKAFMEKREPKFVGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTQRAVQTASSEVLYAVEDHIATITLNAPERMNTISGPMLNELAALLLRANEDADVRC
CCHHHHHHHHCCCEEEEEECCEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEE
VILTGNGRAFCAGLDLRKERGDEGLSAASSPTTLDLRSTPPTVLQAMDKPTICAVNGGAA
EEEECCCEEEEECCCHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCEEEECCCCC
GYGMDTALGCDIRIMAESAKLAAAFVKRGVVPESGGTWFLPRMIGWAKAAELIFTGRTLS
CCCCCHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHEEECCCCC
ARESLDWGLANEVVPDSELMGRARAVAKEIAGNAPLAVQAAKRMMRIGLNETFPDHVHHV
HHHHCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
YLQLLPLFKTQDMAEGIKAFMEKREPKFVGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]