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The map label for this gene is flbD [H]

Identifier: 86748401

GI number: 86748401

Start: 1462058

End: 1463437

Strand: Direct

Name: flbD [H]

Synonym: RPB_1276

Alternate gene names: 86748401

Gene position: 1462058-1463437 (Clockwise)

Preceding gene: 86748400

Following gene: 86748405

Centisome position: 27.42

GC content: 68.12

Gene sequence:

>1380_bases
ATGCGGCTTCTCATCGTCGGCACCCTGAAGGGCCAACTCACAACCGCGACCAAGATCGCGATCGACAACGGCGCGTCCGT
CACCCACGCCGACGACAACGAACAGGCGTTGCGGGTGCTGCGCGGCGGCAAGGGCGCCGATCTGCTGCTGGTCGACGTCG
CGATCGACATCCGCGATCTGGTGATGCGGCTGGAGGCCGAACACATCCACGTGCCGATCGTCGCCTGCGGCATCGCCAAC
GACGCCCGCGCCGCGGTCGCCGCGATCCACGCCGGCGCCAAGGAGTACATCCCGCTGCCGCCGGATCCGGAACTGATCGC
GGCGGTGCTGGCCGCGGTCGCCAATGATTCCCGCGAACTGGTGTATCGAGACGAGGCGATGGCCAAGGTGGTGAAGCTGG
CGCAGCAGATCGCCGGTTCGGACGCCTCGGTGATGATCACCGGCGAGAGCGGCACCGGCAAGGAAGTGCTGGCGCGCTAC
GTCCACACCCGCTCGCACCGGGCGAAGAAGCCGTTCATCAGCATCAATTGCGCGGCGATTCCGGAACACCTGCTCGAATC
CGAATTGTTCGGCCACGAGAAGGGCGCCTTCACCGGCGCGGTGGCGCGCCGCATCGGCAAGTTCGAGGAAGCGACCGGCG
GCACGCTGCTGCTCGACGAAATTTCCGAGATGGACGTGCGGCTGCAGTCGAAGCTGCTGCGCGCGATCCAGGAGCGGGTG
ATCGACCGCGTCGGCGGCAGCCGCCCGGTGCCGGTCGACATCCGCATTCTCGCCACCTCGAACCGCAACCTGCAGGACGC
GGTGCGCGCCGGCACTTTCCGCGAGGATCTGCTGTTCCGGCTCAACGTCGTCAACCTGAAGATTCCGCCGCTGCGTGAAC
GGCCGGCGGATATTCTCGAACTGGCGCAGCATTTCGCCCGCAAATACGCCGACGCCAACGGCGTGCCGGTGCGGCCGATT
TCGGCCGACGCGCGGCGCGTGCTCACCACCAACCGCTGGCAGGGCAACGTCCGTGAGTTGGAGAACACGATTCACCGCGC
GGTGCTGATGGCGCAGGGCGACGAGATCGGCGCCGGTGCGATCCTGACGCCGGACGGCGACCGGCTCGATCAGGCCAAGA
CGCCGCCGGCGGTGGCGCACGCCACCTTCGCGGCCGAGCAGGTGACCCGCGCGCTGGTCGGCCGAACCGTCGCCGACGTC
GAGCGCGACCTGATCCTGGAAACGCTGAAGCACTGCCTCGGCAACCGCACCCACGCGGCGAATATTCTGGGCATCTCGAT
CCGCACGCTGCGCAACAAGCTCAACGAATATGCCGACGGCGGCCTGCCGATTCCGGCCGCCGGCGGCGGCGAACACCGCG
CCTTCGTGCAGACGGCGTGA

Upstream 100 bases:

>100_bases
ACAAGCTCGGCGTCACCATGACCGAAATCATCAAGGCGGAACGCAGCTAATTCGGAAGCCCGCGTCGGCGCGGCAGGACA
CAGGATCACAGGAGCGAACC

Downstream 100 bases:

>100_bases
GGGCGCCGCCAACAAGCACACTGTCATTCCGGGGCGCTCACGAAGTGAGCGAACCCGGAATCCATACGCCGCAGCTTCGC
TATCGAAGCACGGTGGTCGT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRLLIVGTLKGQLTTATKIAIDNGASVTHADDNEQALRVLRGGKGADLLLVDVAIDIRDLVMRLEAEHIHVPIVACGIAN
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRELVYRDEAMAKVVKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSHRAKKPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGSRPVPVDIRILATSNRNLQDAVRAGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYADANGVPVRPI
SADARRVLTTNRWQGNVRELENTIHRAVLMAQGDEIGAGAILTPDGDRLDQAKTPPAVAHATFAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGLPIPAAGGGEHRAFVQTA

Sequences:

>Translated_459_residues
MRLLIVGTLKGQLTTATKIAIDNGASVTHADDNEQALRVLRGGKGADLLLVDVAIDIRDLVMRLEAEHIHVPIVACGIAN
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRELVYRDEAMAKVVKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSHRAKKPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGSRPVPVDIRILATSNRNLQDAVRAGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYADANGVPVRPI
SADARRVLTTNRWQGNVRELENTIHRAVLMAQGDEIGAGAILTPDGDRLDQAKTPPAVAHATFAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGLPIPAAGGGEHRAFVQTA
>Mature_459_residues
MRLLIVGTLKGQLTTATKIAIDNGASVTHADDNEQALRVLRGGKGADLLLVDVAIDIRDLVMRLEAEHIHVPIVACGIAN
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRELVYRDEAMAKVVKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSHRAKKPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGSRPVPVDIRILATSNRNLQDAVRAGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYADANGVPVRPI
SADARRVLTTNRWQGNVRELENTIHRAVLMAQGDEIGAGAILTPDGDRLDQAKTPPAVAHATFAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGLPIPAAGGGEHRAFVQTA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=437, Percent_Identity=41.8764302059497, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790299, Length=444, Percent_Identity=38.2882882882883, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1790437, Length=429, Percent_Identity=38.4615384615385, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082117, Length=239, Percent_Identity=51.4644351464435, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1788905, Length=426, Percent_Identity=38.0281690140845, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1789087, Length=286, Percent_Identity=43.7062937062937, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=42.6791277258567, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=40.1929260450161, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=49.5762711864407, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=39.5569620253165, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.855421686747, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=34.0163934426229, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI87081858, Length=345, Percent_Identity=27.536231884058, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49517; Mature: 49517

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLIVGTLKGQLTTATKIAIDNGASVTHADDNEQALRVLRGGKGADLLLVDVAIDIRDL
CEEEEEEECCCCEEEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHH
VMRLEAEHIHVPIVACGIANDARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSREL
HHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCHHE
VYRDEAMAKVVKLAQQIAGSDASVMITGESGTGKEVLARYVHTRSHRAKKPFISINCAAI
EEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
PEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLLDEISEMDVRLQSKLLRAIQERV
HHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
IDRVGGSRPVPVDIRILATSNRNLQDAVRAGTFREDLLFRLNVVNLKIPPLRERPADILE
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCHHHHH
LAQHFARKYADANGVPVRPISADARRVLTTNRWQGNVRELENTIHRAVLMAQGDEIGAGA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCE
ILTPDGDRLDQAKTPPAVAHATFAAEQVTRALVGRTVADVERDLILETLKHCLGNRTHAA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLNEYADGGLPIPAAGGGEHRAFVQTA
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MRLLIVGTLKGQLTTATKIAIDNGASVTHADDNEQALRVLRGGKGADLLLVDVAIDIRDL
CEEEEEEECCCCEEEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHH
VMRLEAEHIHVPIVACGIANDARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSREL
HHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCHHE
VYRDEAMAKVVKLAQQIAGSDASVMITGESGTGKEVLARYVHTRSHRAKKPFISINCAAI
EEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
PEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLLDEISEMDVRLQSKLLRAIQERV
HHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
IDRVGGSRPVPVDIRILATSNRNLQDAVRAGTFREDLLFRLNVVNLKIPPLRERPADILE
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCHHHHH
LAQHFARKYADANGVPVRPISADARRVLTTNRWQGNVRELENTIHRAVLMAQGDEIGAGA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCE
ILTPDGDRLDQAKTPPAVAHATFAAEQVTRALVGRTVADVERDLILETLKHCLGNRTHAA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLNEYADGGLPIPAAGGGEHRAFVQTA
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]