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The map label for this gene is paaK [H]

Identifier: 86748362

GI number: 86748362

Start: 1415438

End: 1416772

Strand: Direct

Name: paaK [H]

Synonym: RPB_1237

Alternate gene names: 86748362

Gene position: 1415438-1416772 (Clockwise)

Preceding gene: 86748361

Following gene: 86748366

Centisome position: 26.55

GC content: 66.74

Gene sequence:

>1335_bases
ATGAGCGCCCAGCCCGCATTGCGTGAACAGCCCGTCTATATGCACGATCGCGCCATCGAGATGATGCCGCGGCCGCAGCT
CGCGGCGCTGCAGTTCGAGCGGCTGCGCCGAATCGTCGAGCGGGCCTATCGCGACGTCCCGCATTATCGCCGGACGTTCG
ACGAAGCCGGCGTCAAGCCGGCCGATCTGCAGACGCTCGCGGACATCGCGAAGTTTCCGTTCACCAAGAAAAACGATCTG
CGCGACAACTATCCGTTCGGCATGTTCGCGGTGCCGCGCAATCAGCTACCGCGGATTCACGCCTCGTCGGGCACCACCGG
CAAGCCGACCGTGGTCGGTTATACCAAGACCGATCTCGACAACTGGGCCGCCCTGATGGCGCGGTCGATGGTCGGGGCGG
GCGTGTCGCCCGACGACATCGTCCACAACGCCTATGGCTACGGCCTGTTCACTGGTGGGCTCGGCGCGCATTACGGCGCC
GAGCGGCTGGGCTGCACCGTGGTGCCGATCTCCGGCGGCGGCACCGAGCGGCAGGTGACGCTGATGACCGATTTCGGCGC
CAACGTGCTGTGCTGCACGCCGTCCTACGCGCTCAACATCGCCGAAGTCGCCGAGCAGATGGGCGTGAACCTGCGCGCCG
CGCCGCTGCGGATCGGCGTGTTCGGCGCCGAGCCGTGGTCGGATGCGATGCGCCGCGACCTTGAGGCGCGGCTCGGCATC
AAGGCGATCGACGTCTACGGCCTGTCGGAGATCATGGGCCCCGGCGTCGCCTGCGAATGCGCCGTGGCGCAGAACGGTCT
GCACGGCTGGGAAGATCACTTCCTGTTCGAGACCATCGATCCGGAAACCCTGCAGGTGCTGCCGATGGGGTCGGTCGGCG
AATTGGTGATCACCACGCTGACCAAGGAAGCGCTGCCGATGATCCGGTATCGCACCCGCGACATCACCAGCCTGTCGACC
GAACCCTGCGCCTGCGGCCGCACCCATCTGCGGATCATGCGCGTCACCGGCCGTGACGACGACATGCTGATCATCCGCGG
CGTCAACGTCTATCCGTCGCAGGTGGAGTCGGTGCTGGTCGGCTTCCCCGGCATCGCGCCGCACTACCAGATCGTGCTGA
CGCGGGAGAAGGCGCTCGACGCCATGACGGTCGAAGTCGAGATCGCCCCCGACGCCCCGCGCGACGAGGCGGCGCTGGTG
AAGAAGGCGGCCGAGGTCACCCACCACATCAAGTCGCTGATCGGCGTGACCTGCAAGGTCGTAGTGAAGTCGCCGGGCGA
CGTCCCGCGCTCGCAGGGCAAGGCGGTGCGGGTGAAGGATCAGCGGAATATTTGA

Upstream 100 bases:

>100_bases
CCCCAACCCTCTCCCCGCGAAGAGCGGGGCGAGGGAGCTCGCCGTGCGCGGCGCGACCGCATCGTTCTGCATAGTCGGAT
ACTGACAAAGGATTGTTTCG

Downstream 100 bases:

>100_bases
CGGACGGCGACTTCGCTCGTTCCGTTCTCCCGAACTATCCGCACGCGATCCGTCATCCTGAGGTGCTCGCCCGAAGGGCG
AGCCTCGAAGGATGGAGACC

Product: phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSAQPALREQPVYMHDRAIEMMPRPQLAALQFERLRRIVERAYRDVPHYRRTFDEAGVKPADLQTLADIAKFPFTKKNDL
RDNYPFGMFAVPRNQLPRIHASSGTTGKPTVVGYTKTDLDNWAALMARSMVGAGVSPDDIVHNAYGYGLFTGGLGAHYGA
ERLGCTVVPISGGGTERQVTLMTDFGANVLCCTPSYALNIAEVAEQMGVNLRAAPLRIGVFGAEPWSDAMRRDLEARLGI
KAIDVYGLSEIMGPGVACECAVAQNGLHGWEDHFLFETIDPETLQVLPMGSVGELVITTLTKEALPMIRYRTRDITSLST
EPCACGRTHLRIMRVTGRDDDMLIIRGVNVYPSQVESVLVGFPGIAPHYQIVLTREKALDAMTVEVEIAPDAPRDEAALV
KKAAEVTHHIKSLIGVTCKVVVKSPGDVPRSQGKAVRVKDQRNI

Sequences:

>Translated_444_residues
MSAQPALREQPVYMHDRAIEMMPRPQLAALQFERLRRIVERAYRDVPHYRRTFDEAGVKPADLQTLADIAKFPFTKKNDL
RDNYPFGMFAVPRNQLPRIHASSGTTGKPTVVGYTKTDLDNWAALMARSMVGAGVSPDDIVHNAYGYGLFTGGLGAHYGA
ERLGCTVVPISGGGTERQVTLMTDFGANVLCCTPSYALNIAEVAEQMGVNLRAAPLRIGVFGAEPWSDAMRRDLEARLGI
KAIDVYGLSEIMGPGVACECAVAQNGLHGWEDHFLFETIDPETLQVLPMGSVGELVITTLTKEALPMIRYRTRDITSLST
EPCACGRTHLRIMRVTGRDDDMLIIRGVNVYPSQVESVLVGFPGIAPHYQIVLTREKALDAMTVEVEIAPDAPRDEAALV
KKAAEVTHHIKSLIGVTCKVVVKSPGDVPRSQGKAVRVKDQRNI
>Mature_443_residues
SAQPALREQPVYMHDRAIEMMPRPQLAALQFERLRRIVERAYRDVPHYRRTFDEAGVKPADLQTLADIAKFPFTKKNDLR
DNYPFGMFAVPRNQLPRIHASSGTTGKPTVVGYTKTDLDNWAALMARSMVGAGVSPDDIVHNAYGYGLFTGGLGAHYGAE
RLGCTVVPISGGGTERQVTLMTDFGANVLCCTPSYALNIAEVAEQMGVNLRAAPLRIGVFGAEPWSDAMRRDLEARLGIK
AIDVYGLSEIMGPGVACECAVAQNGLHGWEDHFLFETIDPETLQVLPMGSVGELVITTLTKEALPMIRYRTRDITSLSTE
PCACGRTHLRIMRVTGRDDDMLIIRGVNVYPSQVESVLVGFPGIAPHYQIVLTREKALDAMTVEVEIAPDAPRDEAALVK
KAAEVTHHIKSLIGVTCKVVVKSPGDVPRSQGKAVRVKDQRNI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=428, Percent_Identity=54.4392523364486, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48651; Mature: 48520

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQPALREQPVYMHDRAIEMMPRPQLAALQFERLRRIVERAYRDVPHYRRTFDEAGVKP
CCCCCCCCCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
ADLQTLADIAKFPFTKKNDLRDNYPFGMFAVPRNQLPRIHASSGTTGKPTVVGYTKTDLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCEEEEEECCCHH
NWAALMARSMVGAGVSPDDIVHNAYGYGLFTGGLGAHYGAERLGCTVVPISGGGTERQVT
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHCCEEEEEECCCCCCEEEE
LMTDFGANVLCCTPSYALNIAEVAEQMGVNLRAAPLRIGVFGAEPWSDAMRRDLEARLGI
EEEECCCCEEEECCHHHCCHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHHHHHCCE
KAIDVYGLSEIMGPGVACECAVAQNGLHGWEDHFLFETIDPETLQVLPMGSVGELVITTL
EEEEECCHHHHHCCCCEEEEHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHH
TKEALPMIRYRTRDITSLSTEPCACGRTHLRIMRVTGRDDDMLIIRGVNVYPSQVESVLV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHH
GFPGIAPHYQIVLTREKALDAMTVEVEIAPDAPRDEAALVKKAAEVTHHIKSLIGVTCKV
CCCCCCCCEEEEEEHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VVKSPGDVPRSQGKAVRVKDQRNI
EEECCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
SAQPALREQPVYMHDRAIEMMPRPQLAALQFERLRRIVERAYRDVPHYRRTFDEAGVKP
CCCCCCCCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
ADLQTLADIAKFPFTKKNDLRDNYPFGMFAVPRNQLPRIHASSGTTGKPTVVGYTKTDLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCEEEEEECCCHH
NWAALMARSMVGAGVSPDDIVHNAYGYGLFTGGLGAHYGAERLGCTVVPISGGGTERQVT
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHCCEEEEEECCCCCCEEEE
LMTDFGANVLCCTPSYALNIAEVAEQMGVNLRAAPLRIGVFGAEPWSDAMRRDLEARLGI
EEEECCCCEEEECCHHHCCHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHHHHHCCE
KAIDVYGLSEIMGPGVACECAVAQNGLHGWEDHFLFETIDPETLQVLPMGSVGELVITTL
EEEEECCHHHHHCCCCEEEEHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHH
TKEALPMIRYRTRDITSLSTEPCACGRTHLRIMRVTGRDDDMLIIRGVNVYPSQVESVLV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHH
GFPGIAPHYQIVLTREKALDAMTVEVEIAPDAPRDEAALVKKAAEVTHHIKSLIGVTCKV
CCCCCCCCEEEEEEHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VVKSPGDVPRSQGKAVRVKDQRNI
EEECCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]