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The map label for this gene is fabI1 [H]

Identifier: 86748336

GI number: 86748336

Start: 1384102

End: 1384920

Strand: Direct

Name: fabI1 [H]

Synonym: RPB_1211

Alternate gene names: 86748336

Gene position: 1384102-1384920 (Clockwise)

Preceding gene: 86748332

Following gene: 86748337

Centisome position: 25.96

GC content: 64.22

Gene sequence:

>819_bases
ATGGTGACCGGTTCGGGTCTGATGCAAGGCAAGCGTGGGGTGATTCTGGGCGTGGCCAACAACCGCTCGATCGCCTGGGG
CATCGCCAAGGCCTGCCGCGCCCATGGCGCGGAGATCGCGCTGACCTGGCAGGGCGACGCGCTGAAGAAGCGGGTGATGC
CGCTGGCGGCCGAACTCGACGGCATTTTGGTCGGGCATTGCGACGTCACCGATCCGGCCACCATCGATGCGGTGTTCGAC
GAACTGAAGGCGAAATGGGGCAAGATCGACTTCGTCGTCCATGCCATCGCGTTCTCCGACAAGGACCAGCTCGACGGCCG
TTATGTCGAGACCACCGCCGACAACTTCTCCAAGACGATGCTGATCTCGTGCTATTCGCTGACCGCGATCGCGCAGCGCG
CCGAGAAGCTGATGCCGGACGGCGGCTCGATCCTGACGCTGACCTATTACGGCGCCGAGAAGTGGATGCCGCATTACAAC
GTGATGGGCGTCGCCAAGGCCGCGCTCGAAGCCAGCGTGCGTTACCTCGCGGCCGATCTCGGCGAGAAGAACATCCGCGT
CAACGCGATCTCGGCCGGCCCGATCAAGACGCTGGCGGCGTCGGGCATCGGCGACTTCCGCTACATCCTGAAATGGAACG
AATACAATTCGCCGATGCGGCGCACGGTGACGATCGAGGAGGTCGGCGACAGCGCGCTGTATTTCCTGTCCGGCCTGTCG
CGCGGCGTCACCGGCGAAATCCACCACGTCGATTCCGGCTACCACGTCGTCGGCATGAAGCGCCCCGACGCGCCGGACAT
TTCGCTGGCGAAGGACTGA

Upstream 100 bases:

>100_bases
GAATGCGCCAAAACGGCTGAATTTGCTGCGAAAATGGGGGCTGTACCCGGCCCGGCCGCTGGTGTAGGTCCAGAGCCCAA
TCAAGGCGATAGGATACGAC

Downstream 100 bases:

>100_bases
GCGCGGCGACCATCTCCGAGCCATCATCCTGAGGTGCTCGCCCGCAAGGGCGAGCCTCGAAGGATGCGGACACGCGCACA
GCCGGTGCCTTGCGGCCCAT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MVTGSGLMQGKRGVILGVANNRSIAWGIAKACRAHGAEIALTWQGDALKKRVMPLAAELDGILVGHCDVTDPATIDAVFD
ELKAKWGKIDFVVHAIAFSDKDQLDGRYVETTADNFSKTMLISCYSLTAIAQRAEKLMPDGGSILTLTYYGAEKWMPHYN
VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPMRRTVTIEEVGDSALYFLSGLS
RGVTGEIHHVDSGYHVVGMKRPDAPDISLAKD

Sequences:

>Translated_272_residues
MVTGSGLMQGKRGVILGVANNRSIAWGIAKACRAHGAEIALTWQGDALKKRVMPLAAELDGILVGHCDVTDPATIDAVFD
ELKAKWGKIDFVVHAIAFSDKDQLDGRYVETTADNFSKTMLISCYSLTAIAQRAEKLMPDGGSILTLTYYGAEKWMPHYN
VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPMRRTVTIEEVGDSALYFLSGLS
RGVTGEIHHVDSGYHVVGMKRPDAPDISLAKD
>Mature_272_residues
MVTGSGLMQGKRGVILGVANNRSIAWGIAKACRAHGAEIALTWQGDALKKRVMPLAAELDGILVGHCDVTDPATIDAVFD
ELKAKWGKIDFVVHAIAFSDKDQLDGRYVETTADNFSKTMLISCYSLTAIAQRAEKLMPDGGSILTLTYYGAEKWMPHYN
VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPMRRTVTIEEVGDSALYFLSGLS
RGVTGEIHHVDSGYHVVGMKRPDAPDISLAKD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=50.5836575875486, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI1789378, Length=263, Percent_Identity=28.1368821292776, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=28.8537549407115, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787905, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=25, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29435; Mature: 29435

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTGSGLMQGKRGVILGVANNRSIAWGIAKACRAHGAEIALTWQGDALKKRVMPLAAELD
CCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCC
GILVGHCDVTDPATIDAVFDELKAKWGKIDFVVHAIAFSDKDQLDGRYVETTADNFSKTM
CEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCCEE
LISCYSLTAIAQRAEKLMPDGGSILTLTYYGAEKWMPHYNVMGVAKAALEASVRYLAADL
EEHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPMRRTVTIEEVGDSALYFLSGLS
CCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHHCC
RGVTGEIHHVDSGYHVVGMKRPDAPDISLAKD
CCCCCEEEEECCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MVTGSGLMQGKRGVILGVANNRSIAWGIAKACRAHGAEIALTWQGDALKKRVMPLAAELD
CCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCC
GILVGHCDVTDPATIDAVFDELKAKWGKIDFVVHAIAFSDKDQLDGRYVETTADNFSKTM
CEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCCEE
LISCYSLTAIAQRAEKLMPDGGSILTLTYYGAEKWMPHYNVMGVAKAALEASVRYLAADL
EEHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPMRRTVTIEEVGDSALYFLSGLS
CCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHHCC
RGVTGEIHHVDSGYHVVGMKRPDAPDISLAKD
CCCCCEEEEECCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]