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The map label for this gene is rpoE [H]

Identifier: 86748335

GI number: 86748335

Start: 1383408

End: 1383926

Strand: Reverse

Name: rpoE [H]

Synonym: RPB_1210

Alternate gene names: 86748335

Gene position: 1383926-1383408 (Counterclockwise)

Preceding gene: 86748339

Following gene: 86748334

Centisome position: 25.96

GC content: 68.02

Gene sequence:

>519_bases
ATGGAAAGCCAGGTCGGGGCGATCGGAGCGATGAACGAGTTTCGGGACGGGGTCGAGGCGATGATTCCGGCGTTGCGGCG
ATACGCCCGCGCGCTGGCGCGCGACACCGATATTGCCGACGATCTGGTGCAGGACACGCTGGTGCGGGCGCTGCGCTCGG
AAAAGCTGTTTCTCGGCGGCGATTTGCGGAGCTGGCTGTACACCATTCTGACCAATCTCAACCGCAACCGCCGTCGCGCG
CTGGCGCGTCGACCGACCTTGATGCCGTGGACCGATACCGGCGCCGACGCCAGCGGAACCGAGGCCGAGGGCGGCGATAT
CGCCCGTGCCTTGGCGACCCTGGTCGAGGAGCAGCGGGCGGCGCTGCTGCTGGTGGCGCTGGAAGGTCTGAGCTACCGCG
AGGTCGCGGACATTCAGGGGGTGCCGATCGGCACCGTGATGTCCCGTCTCGCCCGCGCCCGCGCCCATATCCGCGCCAGC
CTGGATGGCGAGAAGCCGATGCTGAGGCGGGTGAAATGA

Upstream 100 bases:

>100_bases
CAAATTCAGCCGTTTTGGCGCATTCTTGGGGCGATGGAATAGCTGCCCTGCGCTGGTGTTATGATTCCCGGCGCCGCCGA
CATCGTCGGGACACGGCCGC

Downstream 100 bases:

>100_bases
TGATGCATCGTCAGCCGCAGATCGGGGCCGTCCTCCTGAGAGCCTGCCCCGGACTTGATCCGGGGTGCTCGCCGCTTGCG
GCGAGCCTCGAAGGATGCGC

Product: sigma-24 (FecI-like)

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MESQVGAIGAMNEFRDGVEAMIPALRRYARALARDTDIADDLVQDTLVRALRSEKLFLGGDLRSWLYTILTNLNRNRRRA
LARRPTLMPWTDTGADASGTEAEGGDIARALATLVEEQRAALLLVALEGLSYREVADIQGVPIGTVMSRLARARAHIRAS
LDGEKPMLRRVK

Sequences:

>Translated_172_residues
MESQVGAIGAMNEFRDGVEAMIPALRRYARALARDTDIADDLVQDTLVRALRSEKLFLGGDLRSWLYTILTNLNRNRRRA
LARRPTLMPWTDTGADASGTEAEGGDIARALATLVEEQRAALLLVALEGLSYREVADIQGVPIGTVMSRLARARAHIRAS
LDGEKPMLRRVK
>Mature_172_residues
MESQVGAIGAMNEFRDGVEAMIPALRRYARALARDTDIADDLVQDTLVRALRSEKLFLGGDLRSWLYTILTNLNRNRRRA
LARRPTLMPWTDTGADASGTEAEGGDIARALATLVEEQRAALLLVALEGLSYREVADIQGVPIGTVMSRLARARAHIRAS
LDGEKPMLRRVK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 18970; Mature: 18970

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQVGAIGAMNEFRDGVEAMIPALRRYARALARDTDIADDLVQDTLVRALRSEKLFLGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECC
DLRSWLYTILTNLNRNRRRALARRPTLMPWTDTGADASGTEAEGGDIARALATLVEEQRA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALLLVALEGLSYREVADIQGVPIGTVMSRLARARAHIRASLDGEKPMLRRVK
HHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure
MESQVGAIGAMNEFRDGVEAMIPALRRYARALARDTDIADDLVQDTLVRALRSEKLFLGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECC
DLRSWLYTILTNLNRNRRRALARRPTLMPWTDTGADASGTEAEGGDIARALATLVEEQRA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALLLVALEGLSYREVADIQGVPIGTVMSRLARARAHIRASLDGEKPMLRRVK
HHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]