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The map label for this gene is cca [H]

Identifier: 86748321

GI number: 86748321

Start: 1371230

End: 1372486

Strand: Direct

Name: cca [H]

Synonym: RPB_1196

Alternate gene names: 86748321

Gene position: 1371230-1372486 (Clockwise)

Preceding gene: 86748320

Following gene: 86748325

Centisome position: 25.72

GC content: 71.6

Gene sequence:

>1257_bases
ATGACCGGGGGGGCGGTGCGCGCCGATGCGCCGTGGCTGCATGCCGGCCCGGCCGCTCAGGTTCTCGCGCTGCTCAACCA
CGATGGCGAAGAAGCCCGCGTGGTCGGCGGCGCGGTCCGAAACGCGCTGCTGAAACTGCCGATCGGCGACGTCGACATCG
CAACCACCGCTCTGCCGGACGAAGTCGTCCGCCGCGCCCGGGCCGCCGGCATCAAGGCGGTGCCGACCGGGGTCGAGCAC
GGCACGGTCACGCTGGTGCTGGACGGTCACGGCTTCGAAGTCACCACGCTGCGCGAGGATGTCGAAACCTTCGGCCGCAA
GGCCAAGGTCGCGTTCGGCCGCGACTGGCTGCGCGACGCGCAGCGCCGCGACTTCACCATCAATGGACTGTCGGTGTCGC
CCGACGGCGTGGTGCACGACTATGTCGGCGGACTCGACGACATCGCCGCGCGGCGGGTGCGGTTCATCGGCGATCCGGAT
CAGCGCATCGCAGAAGACTACCTGCGCATCCTGCGCTTCTTCCGCATTCATGCAGCCTACGGCGTCGGCCCGCCGCAGCG
TGCCGGCACGCTCGCCTGCATCCGCGGTCGCGCTGGGCTCGCGACGCTCTCGGCCGAGCGGATGCGGATGGAGATGCTGA
AACTGATGGTGGCGGACGGCGCGCTCGCTTCGGTCGAGGCCATGGCGGACGGCGGTCTTCTGCTCGCGGTGCTCGGCGGC
GTGACCTATCCCGGCCCCTTCGCGGCGATGATCGGGGCGGAACAGGCGCTCGGCCTCGATCCGGATCCGGTGCGGCGGCT
CGGCGCACTCGCGGTCGCGGTCACCGAGGACGCCAAGCGGCTGTCGCAGCGGCTGCGGCTGTCGAACGCCGAAGCGAAGC
GGCTCGACTCCATGGGGCACCGCTGGTGGCGGCTCGCCGGCATGGACGAGGCGACCGCGCGCCGCCGGCTGTACCGGCTC
GGCGAGCCGCGCTTTCACGATCGCATGATGCTGGCCTGGGCGCGGGCCGGGCGCCAGGCCGATCCGGCGCCGTGGCGGAA
CCTGGTCGCCCTGCCGCAACGCTGGCGGCCGCCGGGTTTCCCATTGAAAGCCGCCGATTTCATCGCGCGCGGCTTTGCTG
CCGGGCCGGCGCTCGGCCATGTGCTGACGCTGGCCGAAGACGCCTGGCTCGCCGCGGATTTTCCGGTCGAGGAGGGGCGG
CTGCAGGCAATCGCCGACCAGACCGCGGCGAGGTTCGCACGCGATCATTTGCTGTAG

Upstream 100 bases:

>100_bases
TCAGCCTGCTGCTGCTGGTGCATTATTCCGGCGCGCCGCCCGGTGCGACCTATATGCCGGCGCGAATCGAGGACGGCCGC
CTCGTCCCCGGAGCCGAAAA

Downstream 100 bases:

>100_bases
TCGCGTCCGAGAGATGCGGCCCCTGGACGATGTCCTCGGCCGCGAGACTCAGGGGGTAGACGTGACGAAATTGGACGGAT
GGCGCTCAGGCGACCTTGTT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTGGAVRADAPWLHAGPAAQVLALLNHDGEEARVVGGAVRNALLKLPIGDVDIATTALPDEVVRRARAAGIKAVPTGVEH
GTVTLVLDGHGFEVTTLREDVETFGRKAKVAFGRDWLRDAQRRDFTINGLSVSPDGVVHDYVGGLDDIAARRVRFIGDPD
QRIAEDYLRILRFFRIHAAYGVGPPQRAGTLACIRGRAGLATLSAERMRMEMLKLMVADGALASVEAMADGGLLLAVLGG
VTYPGPFAAMIGAEQALGLDPDPVRRLGALAVAVTEDAKRLSQRLRLSNAEAKRLDSMGHRWWRLAGMDEATARRRLYRL
GEPRFHDRMMLAWARAGRQADPAPWRNLVALPQRWRPPGFPLKAADFIARGFAAGPALGHVLTLAEDAWLAADFPVEEGR
LQAIADQTAARFARDHLL

Sequences:

>Translated_418_residues
MTGGAVRADAPWLHAGPAAQVLALLNHDGEEARVVGGAVRNALLKLPIGDVDIATTALPDEVVRRARAAGIKAVPTGVEH
GTVTLVLDGHGFEVTTLREDVETFGRKAKVAFGRDWLRDAQRRDFTINGLSVSPDGVVHDYVGGLDDIAARRVRFIGDPD
QRIAEDYLRILRFFRIHAAYGVGPPQRAGTLACIRGRAGLATLSAERMRMEMLKLMVADGALASVEAMADGGLLLAVLGG
VTYPGPFAAMIGAEQALGLDPDPVRRLGALAVAVTEDAKRLSQRLRLSNAEAKRLDSMGHRWWRLAGMDEATARRRLYRL
GEPRFHDRMMLAWARAGRQADPAPWRNLVALPQRWRPPGFPLKAADFIARGFAAGPALGHVLTLAEDAWLAADFPVEEGR
LQAIADQTAARFARDHLL
>Mature_417_residues
TGGAVRADAPWLHAGPAAQVLALLNHDGEEARVVGGAVRNALLKLPIGDVDIATTALPDEVVRRARAAGIKAVPTGVEHG
TVTLVLDGHGFEVTTLREDVETFGRKAKVAFGRDWLRDAQRRDFTINGLSVSPDGVVHDYVGGLDDIAARRVRFIGDPDQ
RIAEDYLRILRFFRIHAAYGVGPPQRAGTLACIRGRAGLATLSAERMRMEMLKLMVADGALASVEAMADGGLLLAVLGGV
TYPGPFAAMIGAEQALGLDPDPVRRLGALAVAVTEDAKRLSQRLRLSNAEAKRLDSMGHRWWRLAGMDEATARRRLYRLG
EPRFHDRMMLAWARAGRQADPAPWRNLVALPQRWRPPGFPLKAADFIARGFAAGPALGHVLTLAEDAWLAADFPVEEGRL
QAIADQTAARFARDHLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=394, Percent_Identity=31.4720812182741, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI87081691, Length=246, Percent_Identity=32.1138211382114, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=379, Percent_Identity=29.8153034300792, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI281360112, Length=388, Percent_Identity=31.4432989690722, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357337, Length=388, Percent_Identity=31.4432989690722, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45283; Mature: 45151

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGAVRADAPWLHAGPAAQVLALLNHDGEEARVVGGAVRNALLKLPIGDVDIATTALPD
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
EVVRRARAAGIKAVPTGVEHGTVTLVLDGHGFEVTTLREDVETFGRKAKVAFGRDWLRDA
HHHHHHHHCCCEECCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCHHHCHHHHHHH
QRRDFTINGLSVSPDGVVHDYVGGLDDIAARRVRFIGDPDQRIAEDYLRILRFFRIHAAY
HHCCEEEECCEECCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GVGPPQRAGTLACIRGRAGLATLSAERMRMEMLKLMVADGALASVEAMADGGLLLAVLGG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCC
VTYPGPFAAMIGAEQALGLDPDPVRRLGALAVAVTEDAKRLSQRLRLSNAEAKRLDSMGH
CCCCCHHHHHHCCHHHCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
RWWRLAGMDEATARRRLYRLGEPRFHDRMMLAWARAGRQADPAPWRNLVALPQRWRPPGF
HEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCC
PLKAADFIARGFAAGPALGHVLTLAEDAWLAADFPVEEGRLQAIADQTAARFARDHLL
CCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGGAVRADAPWLHAGPAAQVLALLNHDGEEARVVGGAVRNALLKLPIGDVDIATTALPD
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
EVVRRARAAGIKAVPTGVEHGTVTLVLDGHGFEVTTLREDVETFGRKAKVAFGRDWLRDA
HHHHHHHHCCCEECCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCHHHCHHHHHHH
QRRDFTINGLSVSPDGVVHDYVGGLDDIAARRVRFIGDPDQRIAEDYLRILRFFRIHAAY
HHCCEEEECCEECCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GVGPPQRAGTLACIRGRAGLATLSAERMRMEMLKLMVADGALASVEAMADGGLLLAVLGG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCC
VTYPGPFAAMIGAEQALGLDPDPVRRLGALAVAVTEDAKRLSQRLRLSNAEAKRLDSMGH
CCCCCHHHHHHCCHHHCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
RWWRLAGMDEATARRRLYRLGEPRFHDRMMLAWARAGRQADPAPWRNLVALPQRWRPPGF
HEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCC
PLKAADFIARGFAAGPALGHVLTLAEDAWLAADFPVEEGRLQAIADQTAARFARDHLL
CCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA