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The map label for this gene is cspA [C]

Identifier: 86748304

GI number: 86748304

Start: 1352956

End: 1353168

Strand: Reverse

Name: cspA [C]

Synonym: RPB_1179

Alternate gene names: 86748304

Gene position: 1353168-1352956 (Counterclockwise)

Preceding gene: 86748305

Following gene: 86748302

Centisome position: 25.38

GC content: 53.99

Gene sequence:

>213_bases
ATGCCTAATACGGGAACGGTGAAGTTCTTCAATGGAGAACGCGGCTACGGGTTCATCAAGCCTGATGACGGTGGCCGCGA
CGTATTTGTTCATATCACTGCGGTCGAGCGTGCCGGCTTGAAGGATCTGACCGAAGGTCAGCGCATTTCATTCGAGGTCG
AACCGGACAAAAAGGGCAAAGGCCCCAAGGCGGTCAATCTGGTGATTTCGTAG

Upstream 100 bases:

>100_bases
ATCAGCAGGCCCAGCCTCGGCCGAGCTTTCCGGCAGCGCAACGCCAGGTCGCACCGACGCGCCGGCGCGCGACGGAAAGC
ATGCGGGAGGAGTTTCCGGA

Downstream 100 bases:

>100_bases
TTGTTGTCATGGGCTGTGCAAAACTGAACGCAGCCACGACGAACGCGACGGGCTTTGAAGTGACGGCGTAGCGAATACGA
CGCCGCGGCGCTCGCATGTG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MPNTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVIS

Sequences:

>Translated_70_residues
MPNTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVIS
>Mature_69_residues
PNTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVIS

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=52.3076923076923, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=50, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=46.2686567164179, Blast_Score=72, Evalue=6e-15,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=47.6923076923077, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=50.7936507936508, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=50.7692307692308, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7636; Mature: 7505

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
GPKAVNLVIS
CCEEEEEEEC
>Mature Secondary Structure 
PNTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGK
CCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
GPKAVNLVIS
CCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]