Click here to switch to the map view.

The map label for this gene is dadA3 [H]

Identifier: 86748249

GI number: 86748249

Start: 1294719

End: 1295954

Strand: Direct

Name: dadA3 [H]

Synonym: RPB_1124

Alternate gene names: 86748249

Gene position: 1294719-1295954 (Clockwise)

Preceding gene: 86748248

Following gene: 86748250

Centisome position: 24.28

GC content: 65.7

Gene sequence:

>1236_bases
ATGTCGAAGATCGCTATCATCGGAGCCGGGATCACCGGCGTCACAACAGCTTTCACGCTCCTCGAACGCGGCCATCGCGT
GACCGTGTTCGACCGGCATCGCTACGCCGCGATGGAGACCTCCTATGCCAATGGCGGCCAACTCTCCGCGAGCAACGCCG
AGGTCTGGAACAACTGGGCGACCGTGCGGAAGGGGCTCGGCTGGCTGTTCCGGAAAGACGCGCCGTTGCTGCTCGATCTG
ACGCCGAGCTGGCATAAATACTCCTGGCTGACGGAATTCCTGGTCGGCATCCGGAAGTACGAGAAGAACTCGATCGAGAC
CACCAAGCTCGCGATCGAAGCCCGCCGGCATTTGTTGCGCATGGCGAAGACGGCGTCGGTCGATTTCGACATCGAGACCC
GCGGCATCCTGCACGTCTATCACGACGCGCAGGATTTCGCCCAGGCGGCGAAGTCCAACAAGATGCTGCAGGCGGGCGGC
CTCGAGCGCAGGCCGGTCACCCGCCAGGAAATCCGCGCGATCGAGCCCGCATTGACCGGCAAGTTTCACGGCGGGTTCTA
CACGCCGTCGGATTTCAGCGGCGACATCCACAAATTCGTCACCGGGTTGACGGCGTATTGCGCGCACAACGGCGTCGAGT
TCCGGCTCGGCAAGAACGCGGAGGTGAGCGCCGAAAGGCACGGCGGAATCCGCGTCGACTGGTCGTCGGAGCCGATCGGT
TCGCCTGGCGGGTTCGCGTTAAAGGAGAGCTTCGACGCCGTGGTGGTCTGCGCGGGCGTCGAAAGCCGGCTGCTGGCGAT
GGCGCTGGGAGACCGGGTCAACGTCTACCCCGTGAAAGGCTATTCGATCACCGTGCAGCTCGACAGCGAGTCCGACCGGG
CCGCAGCACCGCACGTGAGCCTGCTCGACGAGCGCGCGAAGATCGTCACCAGCCGGCTCGGCAGCGACCGCTTCCGGGTC
GCCGGCACCGCCGAGATCAACGGGTTCAACCGCGATATCAGGTCGGATCGGATCAGGCCGCTGGTCGACTGGGTGGAGCG
CGTCTTTCCAGGCGTGAGCACGCGCCGGGTCGTGCCCTGGAGCGGGCTACGGCCGATGATGCCCGACATGATGCCGCGCG
TCGGGCGTGGCCGGCGCAAGGGCGTGTTCTACAACACCGGGCATGGTCACCTCGGTTGGACGTTGTCGGCGGCCACGGCG
GAGCTCGTCGCGATGCAGATCGGCCCAGCCGGCTGA

Upstream 100 bases:

>100_bases
GAAGGGGAAGGCCGGGCAATTCGGCTAAGCTCTCAGGTAAAGCGAACAGATGGGGACGCGACCGAGGTTCTCGGTCAGAT
CGAACAATGCGAGTCCCCCT

Downstream 100 bases:

>100_bases
TCCGCCTCCGGCGACGGCGCGGTCCAACTCCGCCCTGCGTCGCTTTGTCCGAGCCTCCGGGGAACTCCGGTGGGGGATTT
TGCGTATCCCTGCCGCGAGC

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSKIAIIGAGITGVTTAFTLLERGHRVTVFDRHRYAAMETSYANGGQLSASNAEVWNNWATVRKGLGWLFRKDAPLLLDL
TPSWHKYSWLTEFLVGIRKYEKNSIETTKLAIEARRHLLRMAKTASVDFDIETRGILHVYHDAQDFAQAAKSNKMLQAGG
LERRPVTRQEIRAIEPALTGKFHGGFYTPSDFSGDIHKFVTGLTAYCAHNGVEFRLGKNAEVSAERHGGIRVDWSSEPIG
SPGGFALKESFDAVVVCAGVESRLLAMALGDRVNVYPVKGYSITVQLDSESDRAAAPHVSLLDERAKIVTSRLGSDRFRV
AGTAEINGFNRDIRSDRIRPLVDWVERVFPGVSTRRVVPWSGLRPMMPDMMPRVGRGRRKGVFYNTGHGHLGWTLSAATA
ELVAMQIGPAG

Sequences:

>Translated_411_residues
MSKIAIIGAGITGVTTAFTLLERGHRVTVFDRHRYAAMETSYANGGQLSASNAEVWNNWATVRKGLGWLFRKDAPLLLDL
TPSWHKYSWLTEFLVGIRKYEKNSIETTKLAIEARRHLLRMAKTASVDFDIETRGILHVYHDAQDFAQAAKSNKMLQAGG
LERRPVTRQEIRAIEPALTGKFHGGFYTPSDFSGDIHKFVTGLTAYCAHNGVEFRLGKNAEVSAERHGGIRVDWSSEPIG
SPGGFALKESFDAVVVCAGVESRLLAMALGDRVNVYPVKGYSITVQLDSESDRAAAPHVSLLDERAKIVTSRLGSDRFRV
AGTAEINGFNRDIRSDRIRPLVDWVERVFPGVSTRRVVPWSGLRPMMPDMMPRVGRGRRKGVFYNTGHGHLGWTLSAATA
ELVAMQIGPAG
>Mature_410_residues
SKIAIIGAGITGVTTAFTLLERGHRVTVFDRHRYAAMETSYANGGQLSASNAEVWNNWATVRKGLGWLFRKDAPLLLDLT
PSWHKYSWLTEFLVGIRKYEKNSIETTKLAIEARRHLLRMAKTASVDFDIETRGILHVYHDAQDFAQAAKSNKMLQAGGL
ERRPVTRQEIRAIEPALTGKFHGGFYTPSDFSGDIHKFVTGLTAYCAHNGVEFRLGKNAEVSAERHGGIRVDWSSEPIGS
PGGFALKESFDAVVVCAGVESRLLAMALGDRVNVYPVKGYSITVQLDSESDRAAAPHVSLLDERAKIVTSRLGSDRFRVA
GTAEINGFNRDIRSDRIRPLVDWVERVFPGVSTRRVVPWSGLRPMMPDMMPRVGRGRRKGVFYNTGHGHLGWTLSAATAE
LVAMQIGPAG

Specific function: Oxidative deamination of D-amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=411, Percent_Identity=31.6301703163017, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45393; Mature: 45262

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIAIIGAGITGVTTAFTLLERGHRVTVFDRHRYAAMETSYANGGQLSASNAEVWNNWA
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCHHHHHHH
TVRKGLGWLFRKDAPLLLDLTPSWHKYSWLTEFLVGIRKYEKNSIETTKLAIEARRHLLR
HHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAKTASVDFDIETRGILHVYHDAQDFAQAAKSNKMLQAGGLERRPVTRQEIRAIEPALTG
HHHHCCCCEEECCCCEEEEEECHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCHHHCC
KFHGGFYTPSDFSGDIHKFVTGLTAYCAHNGVEFRLGKNAEVSAERHGGIRVDWSSEPIG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHCCCCEEEEECCCCCC
SPGGFALKESFDAVVVCAGVESRLLAMALGDRVNVYPVKGYSITVQLDSESDRAAAPHVS
CCCCCHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCHH
LLDERAKIVTSRLGSDRFRVAGTAEINGFNRDIRSDRIRPLVDWVERVFPGVSTRRVVPW
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCEECCC
SGLRPMMPDMMPRVGRGRRKGVFYNTGHGHLGWTLSAATAELVAMQIGPAG
CCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEEHHHHEEEEEEECCCCC
>Mature Secondary Structure 
SKIAIIGAGITGVTTAFTLLERGHRVTVFDRHRYAAMETSYANGGQLSASNAEVWNNWA
CCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCHHHHHHH
TVRKGLGWLFRKDAPLLLDLTPSWHKYSWLTEFLVGIRKYEKNSIETTKLAIEARRHLLR
HHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAKTASVDFDIETRGILHVYHDAQDFAQAAKSNKMLQAGGLERRPVTRQEIRAIEPALTG
HHHHCCCCEEECCCCEEEEEECHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCHHHCC
KFHGGFYTPSDFSGDIHKFVTGLTAYCAHNGVEFRLGKNAEVSAERHGGIRVDWSSEPIG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHCCCCEEEEECCCCCC
SPGGFALKESFDAVVVCAGVESRLLAMALGDRVNVYPVKGYSITVQLDSESDRAAAPHVS
CCCCCHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCHH
LLDERAKIVTSRLGSDRFRVAGTAEINGFNRDIRSDRIRPLVDWVERVFPGVSTRRVVPW
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCEECCC
SGLRPMMPDMMPRVGRGRRKGVFYNTGHGHLGWTLSAATAELVAMQIGPAG
CCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEEHHHHEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]