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The map label for this gene is rpfC [H]

Identifier: 86747941

GI number: 86747941

Start: 931824

End: 933038

Strand: Direct

Name: rpfC [H]

Synonym: RPB_0815

Alternate gene names: 86747941

Gene position: 931824-933038 (Clockwise)

Preceding gene: 86747940

Following gene: 86747943

Centisome position: 17.48

GC content: 71.36

Gene sequence:

>1215_bases
ATGGCAGTGAAGTCGCGCGCCTTGCGCCGCGGGCGGACGCCGGTCAAGCGGTCGCCGATCAAGCGTGCCCGCAAGAAGGC
CGAAACGGCCGGCATGGTCGAGACGGCGCTGGCCGCGTTCGCGCATGAAGTCCGCACCCCGCTCACCGGCATCCTCGCCG
TCAGCGACCTGCTGGCGACCTCCGACCTCGGCGAACGCGAGCGCCGCTGGGTCGACACCATCAAGGCCGGCGCCGAGCAT
CTATCTGGATTGGCGACGCTTTTCGTCGATGCCGCGCGGCTCGGCGCGACCGGCCTGGAGGGCCGCAGCGAGTTCTTCGA
TCTGCGCGCGCTGGCGCGCCATGCCGGCGACTCGCTGTCCGGCCGCGCCGCCGCCAAGGGCCTGCGCGCCAAGGTGACGA
TCTCGCCGCGGCTGCCGGCCTTCGTGGTCGGCGACATGGTGCGGCTGCGCGCGGCGCTGGAAAATCTGATCGACAACGCG
GTGAAGTTCACCGAGCAAGGCGACGTCGCGCTCAAGGTCACCTCGGCGGCGGCCGGCAAGGACAAGGCCGATGTGAGTTT
CGCGGTGTCCGACAGCGGCATCGGACTGACCCTGACCGAGATCAGGCGGCTGTTCCGCCCGTTCTCGCAGGCCAATGTCA
GCATCGCGTCGCGGTTCGGCGGCGCCGGCCTCGGCCTCTCCTCGGTGCGTCAGATCGCCCGGGCGATGCAGGGCGATGTC
GCTGTGGCGCAGGGGGCGAAGGGCGGCGCGGTGTTCACCCTCACGGTCCGGTTGACGCGGACGAAACCGCCGCAGCAGGC
GGCGCCGTCGCACGCCGCCAAAGGCGCCTGCGTGCGGCCGATGCGCCTGCTCAGCGTCGAGGACAATCCGTTCGGCCGCG
TCGTGCTCAACGCCATCCTCACCGAACTCGGCCATCACGCCGAATTCGTCGGCAGCGGCGAACTGGCGCCGGAACGGCTG
GCGCAAGGCGGTTTCGATGCCGTGCTGATGGACATGGTGCTGCCCGGCATCAGCGGGCCCGAGGCCATTCGCAGCATCCG
CGCCCTCGCGGCGCCGCTCGGCGCGGTGCCGATCATCGGCGTCTCCGGCCGCGATCAGGACGAGGCCGCGGCGCGGATGG
CGGGCGCCGACGCCTTTCTGCTCAAGCCGGTGTCTCCCCGCGCGCTCGCAACTGCGCTGCTTGCAGCGTCACGCCGTCCG
GCAGCCGCGACTTGA

Upstream 100 bases:

>100_bases
CGAACTTTGCCGCAAGCCGGACAGCCCCGAAATCTCGTTTTCCCGATGGCTCCGCCAAGGCCGCCATGGCATGGTGCCGG
GCGTTGGGGTCGGGGGAACC

Downstream 100 bases:

>100_bases
TGATGGCGGCGTTGAGTTCGCCGCCATAGACGAAGATCGCCGCGATGAAATACAGAAACACCAGGGCGATGATCACCGAG
GCGAGACCCGCATACATCGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAVKSRALRRGRTPVKRSPIKRARKKAETAGMVETALAAFAHEVRTPLTGILAVSDLLATSDLGERERRWVDTIKAGAEH
LSGLATLFVDAARLGATGLEGRSEFFDLRALARHAGDSLSGRAAAKGLRAKVTISPRLPAFVVGDMVRLRAALENLIDNA
VKFTEQGDVALKVTSAAAGKDKADVSFAVSDSGIGLTLTEIRRLFRPFSQANVSIASRFGGAGLGLSSVRQIARAMQGDV
AVAQGAKGGAVFTLTVRLTRTKPPQQAAPSHAAKGACVRPMRLLSVEDNPFGRVVLNAILTELGHHAEFVGSGELAPERL
AQGGFDAVLMDMVLPGISGPEAIRSIRALAAPLGAVPIIGVSGRDQDEAAARMAGADAFLLKPVSPRALATALLAASRRP
AAAT

Sequences:

>Translated_404_residues
MAVKSRALRRGRTPVKRSPIKRARKKAETAGMVETALAAFAHEVRTPLTGILAVSDLLATSDLGERERRWVDTIKAGAEH
LSGLATLFVDAARLGATGLEGRSEFFDLRALARHAGDSLSGRAAAKGLRAKVTISPRLPAFVVGDMVRLRAALENLIDNA
VKFTEQGDVALKVTSAAAGKDKADVSFAVSDSGIGLTLTEIRRLFRPFSQANVSIASRFGGAGLGLSSVRQIARAMQGDV
AVAQGAKGGAVFTLTVRLTRTKPPQQAAPSHAAKGACVRPMRLLSVEDNPFGRVVLNAILTELGHHAEFVGSGELAPERL
AQGGFDAVLMDMVLPGISGPEAIRSIRALAAPLGAVPIIGVSGRDQDEAAARMAGADAFLLKPVSPRALATALLAASRRP
AAAT
>Mature_403_residues
AVKSRALRRGRTPVKRSPIKRARKKAETAGMVETALAAFAHEVRTPLTGILAVSDLLATSDLGERERRWVDTIKAGAEHL
SGLATLFVDAARLGATGLEGRSEFFDLRALARHAGDSLSGRAAAKGLRAKVTISPRLPAFVVGDMVRLRAALENLIDNAV
KFTEQGDVALKVTSAAAGKDKADVSFAVSDSGIGLTLTEIRRLFRPFSQANVSIASRFGGAGLGLSSVRQIARAMQGDVA
VAQGAKGGAVFTLTVRLTRTKPPQQAAPSHAAKGACVRPMRLLSVEDNPFGRVVLNAILTELGHHAEFVGSGELAPERLA
QGGFDAVLMDMVLPGISGPEAIRSIRALAAPLGAVPIIGVSGRDQDEAAARMAGADAFLLKPVSPRALATALLAASRRPA
AAT

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=366, Percent_Identity=29.7814207650273, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789149, Length=276, Percent_Identity=30.4347826086957, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081816, Length=385, Percent_Identity=30.6493506493506, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1788713, Length=367, Percent_Identity=27.5204359673025, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693157, Length=230, Percent_Identity=30.8695652173913, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=28.4552845528455, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=27.0742358078603, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788393, Length=228, Percent_Identity=27.6315789473684, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42131; Mature: 42000

Theoretical pI: Translated: 11.43; Mature: 11.43

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKSRALRRGRTPVKRSPIKRARKKAETAGMVETALAAFAHEVRTPLTGILAVSDLLAT
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
SDLGERERRWVDTIKAGAEHLSGLATLFVDAARLGATGLEGRSEFFDLRALARHAGDSLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
GRAAAKGLRAKVTISPRLPAFVVGDMVRLRAALENLIDNAVKFTEQGDVALKVTSAAAGK
CHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
DKADVSFAVSDSGIGLTLTEIRRLFRPFSQANVSIASRFGGAGLGLSSVRQIARAMQGDV
CCCCEEEEEECCCCCEEHHHHHHHHCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCC
AVAQGAKGGAVFTLTVRLTRTKPPQQAAPSHAAKGACVRPMRLLSVEDNPFGRVVLNAIL
EEECCCCCCEEEEEEEEEECCCCCHHCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
TELGHHAEFVGSGELAPERLAQGGFDAVLMDMVLPGISGPEAIRSIRALAAPLGAVPIIG
HHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VSGRDQDEAAARMAGADAFLLKPVSPRALATALLAASRRPAAAT
CCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVKSRALRRGRTPVKRSPIKRARKKAETAGMVETALAAFAHEVRTPLTGILAVSDLLAT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
SDLGERERRWVDTIKAGAEHLSGLATLFVDAARLGATGLEGRSEFFDLRALARHAGDSLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
GRAAAKGLRAKVTISPRLPAFVVGDMVRLRAALENLIDNAVKFTEQGDVALKVTSAAAGK
CHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
DKADVSFAVSDSGIGLTLTEIRRLFRPFSQANVSIASRFGGAGLGLSSVRQIARAMQGDV
CCCCEEEEEECCCCCEEHHHHHHHHCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCC
AVAQGAKGGAVFTLTVRLTRTKPPQQAAPSHAAKGACVRPMRLLSVEDNPFGRVVLNAIL
EEECCCCCCEEEEEEEEEECCCCCHHCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
TELGHHAEFVGSGELAPERLAQGGFDAVLMDMVLPGISGPEAIRSIRALAAPLGAVPIIG
HHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VSGRDQDEAAARMAGADAFLLKPVSPRALATALLAASRRPAAAT
CCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]