Click here to switch to the map view.

The map label for this gene is cobW [H]

Identifier: 86747865

GI number: 86747865

Start: 831979

End: 832986

Strand: Reverse

Name: cobW [H]

Synonym: RPB_0739

Alternate gene names: 86747865

Gene position: 832986-831979 (Counterclockwise)

Preceding gene: 86747866

Following gene: 86747864

Centisome position: 15.62

GC content: 70.14

Gene sequence:

>1008_bases
ATGTCCGCCCGTGTCCCCGTCACCGTGCTCACCGGCTTTCTCGGCGCCGGCAAGACCACGCTGCTGCGCGCGCTGCTCAA
TCAGTCGAGCGGCCGCCGCATCGCCGTGATCGTCAACGAATTCGGCGACGCCGGCTTCGACGGCGCGCTGGTCGAGGATT
GCGGTGACGCGGTCTGCGCGCCCGGCGACATCGTCGAACTCACCAATGGCTGCATCTGCTGCACCGTGGCCGACGATTTC
ATTCCGACGATGGAGAAGCTGTTGAATCGCGAGCGCCCGCTCGACGCCATCGTGATCGAGACCTCCGGGCTGGCGCTGCC
GCAACCCTTGCTCAAGGCGTTCGCCTGGCCCGCGGTGAAGACCCGCGCCACCGCCGACGGCGTCGTCACCGTGGTCGACG
CGCTGGCGCTGGTCGAGGGCCGGATTCCGCTCGACGACGATCACGTCAAGGCGCTGCGCGACGTCAGCCATGACGACCCG
ATCGAAGAGGTGTTCGAGGATCAGCTCGCCTGCGCCGATCTGGTGGTGCTGTCGAAGGCCGATCTGGTGACGCCGGAACA
ACTCGCAGAGCTCGAGGCGAAGCTCAAGGCCAAGTTGCGGCCCAACGTCCGCATCGTGCCGTCGCACGGCGCGCTGGCCC
CGTCGGTGCTGATCGGGCTCGGCTCCGCCGCCGAGGACGATCTCGCCGCCCGCGCCGGGCATCACGCCGAAGGCGAAGAG
CACGACCACGACGATTTCGATTCGATCGTGGTGACGCCGCAGCCGGCGGACGATATCGACGCCATGCGCAGCCGCGTCGG
CGCAGCGCTCGGCCTCGACGGCGTGCTGCGGGTCAAGGGCCATGCCCGGATCGCCGGCAAGCCGGCGCCGGTGGTGGTGC
AGGCGGTCGGCACCCGCATCGACCTCGCCTTCGCCCGCCCCGACGCGCAGCGCGCCGACCATCTGGTGGTGATCGGCCTG
AAAGGCTTCGACGCCGACGCCGCGCGGCGCGCGCTGGCGGGCGCGTAG

Upstream 100 bases:

>100_bases
ATGCCCAGGGCCGGCTCAACCAGACCATCGCCGCCGCCGTCCCCCATGTCGTGTTCGTCGCAGCCGGCCTGCCGCTGTGG
CTGAAGCAGGAGAGCTGATC

Downstream 100 bases:

>100_bases
CAGCATGCAAACCTCCCGGGCTCGTCATCCTGAGGCGCACGCCGTCTTCGGCGTGCCTCGAAGGACGACGAGCGTCGCGG
CCCGCGGCCATCCTTCGAGG

Product: cobalamin biosynthesis CobW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSARVPVTVLTGFLGAGKTTLLRALLNQSSGRRIAVIVNEFGDAGFDGALVEDCGDAVCAPGDIVELTNGCICCTVADDF
IPTMEKLLNRERPLDAIVIETSGLALPQPLLKAFAWPAVKTRATADGVVTVVDALALVEGRIPLDDDHVKALRDVSHDDP
IEEVFEDQLACADLVVLSKADLVTPEQLAELEAKLKAKLRPNVRIVPSHGALAPSVLIGLGSAAEDDLAARAGHHAEGEE
HDHDDFDSIVVTPQPADDIDAMRSRVGAALGLDGVLRVKGHARIAGKPAPVVVQAVGTRIDLAFARPDAQRADHLVVIGL
KGFDADAARRALAGA

Sequences:

>Translated_335_residues
MSARVPVTVLTGFLGAGKTTLLRALLNQSSGRRIAVIVNEFGDAGFDGALVEDCGDAVCAPGDIVELTNGCICCTVADDF
IPTMEKLLNRERPLDAIVIETSGLALPQPLLKAFAWPAVKTRATADGVVTVVDALALVEGRIPLDDDHVKALRDVSHDDP
IEEVFEDQLACADLVVLSKADLVTPEQLAELEAKLKAKLRPNVRIVPSHGALAPSVLIGLGSAAEDDLAARAGHHAEGEE
HDHDDFDSIVVTPQPADDIDAMRSRVGAALGLDGVLRVKGHARIAGKPAPVVVQAVGTRIDLAFARPDAQRADHLVVIGL
KGFDADAARRALAGA
>Mature_334_residues
SARVPVTVLTGFLGAGKTTLLRALLNQSSGRRIAVIVNEFGDAGFDGALVEDCGDAVCAPGDIVELTNGCICCTVADDFI
PTMEKLLNRERPLDAIVIETSGLALPQPLLKAFAWPAVKTRATADGVVTVVDALALVEGRIPLDDDHVKALRDVSHDDPI
EEVFEDQLACADLVVLSKADLVTPEQLAELEAKLKAKLRPNVRIVPSHGALAPSVLIGLGSAAEDDLAARAGHHAEGEEH
DHDDFDSIVVTPQPADDIDAMRSRVGAALGLDGVLRVKGHARIAGKPAPVVVQAVGTRIDLAFARPDAQRADHLVVIGLK
GFDADAARRALAGA

Specific function: Might be involved in cobalt reduction leading to cobalt(I) corrinoids [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI223941779, Length=351, Percent_Identity=28.4900284900285, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI33469141, Length=359, Percent_Identity=27.8551532033426, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI126722884, Length=359, Percent_Identity=27.8551532033426, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI148727351, Length=359, Percent_Identity=27.8551532033426, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI146231952, Length=358, Percent_Identity=27.3743016759777, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI119120938, Length=137, Percent_Identity=37.2262773722628, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI223941776, Length=345, Percent_Identity=26.9565217391304, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI87082430, Length=345, Percent_Identity=31.8840579710145, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788499, Length=187, Percent_Identity=32.0855614973262, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324356, Length=219, Percent_Identity=31.9634703196347, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012824
- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 35135; Mature: 35004

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARVPVTVLTGFLGAGKTTLLRALLNQSSGRRIAVIVNEFGDAGFDGALVEDCGDAVCA
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCC
PGDIVELTNGCICCTVADDFIPTMEKLLNRERPLDAIVIETSGLALPQPLLKAFAWPAVK
CCCHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHH
TRATADGVVTVVDALALVEGRIPLDDDHVKALRDVSHDDPIEEVFEDQLACADLVVLSKA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DLVTPEQLAELEAKLKAKLRPNVRIVPSHGALAPSVLIGLGSAAEDDLAARAGHHAEGEE
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
HDHDDFDSIVVTPQPADDIDAMRSRVGAALGLDGVLRVKGHARIAGKPAPVVVQAVGTRI
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCEEEEECCCEE
DLAFARPDAQRADHLVVIGLKGFDADAARRALAGA
EEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
SARVPVTVLTGFLGAGKTTLLRALLNQSSGRRIAVIVNEFGDAGFDGALVEDCGDAVCA
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCC
PGDIVELTNGCICCTVADDFIPTMEKLLNRERPLDAIVIETSGLALPQPLLKAFAWPAVK
CCCHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHH
TRATADGVVTVVDALALVEGRIPLDDDHVKALRDVSHDDPIEEVFEDQLACADLVVLSKA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DLVTPEQLAELEAKLKAKLRPNVRIVPSHGALAPSVLIGLGSAAEDDLAARAGHHAEGEE
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
HDHDDFDSIVVTPQPADDIDAMRSRVGAALGLDGVLRVKGHARIAGKPAPVVVQAVGTRI
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCEEEEECCCEE
DLAFARPDAQRADHLVVIGLKGFDADAARRALAGA
EEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1655697 [H]