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The map label for this gene is cobQ [H]

Identifier: 86747862

GI number: 86747862

Start: 825667

End: 827151

Strand: Reverse

Name: cobQ [H]

Synonym: RPB_0736

Alternate gene names: 86747862

Gene position: 827151-825667 (Counterclockwise)

Preceding gene: 86747863

Following gene: 86747854

Centisome position: 15.51

GC content: 70.44

Gene sequence:

>1485_bases
ATGACCCCCACCCCCGCGCTGATGATCCAGGGCACCTGCTCCAATGCCGGCAAGTCGACGCTGGTGGCGGGGCTGTGCCG
GGCGCTGGTGCGGCGTGGACTACGCGTCGCGCCGTTCAAGCCGCAGAACATGTCGAACAACGCCGCCGTCACCGCCGATG
GCGGCGAGATCGGCCGCGCCCAAGCGGTGCAGGCGCGCGCAGCGAAGCTGCCGCCGAGTGTGCACATGAATCCGGTGCTG
CTGAAACCCGAGACCGACACCGGCGCGCAGGTGATCGTGCAGGGCCGGCGCTTCGGCCGGCTCGGCGCCAAAAACTTTCT
GGACAAGAAGGCGGCGCTGCTGCCGCACGTGCTGGACAGCTTCGCGCGGCTGGCCCGCGACGCCGATATCGTGCTGATCG
AGGGCGCGGGCTCCCCGGCGGAAATCAATCTGCGCGCCGGCGACATCGCCAATATGGGCTTCGCGCTGGCCGCCAATGTC
CCGGTGGTGATCGCCGGCGACATCGAACGCGGCGGCGTGATCGCCAGCCTCGCCGGCACCCATCTGGTGCTCGACGACGA
CGAACGCGCGCTGGTGCGCGGCTATCTGATCAACAAATTCCGCGGCGACGTTCGCCTGTTCGACCACGGCCTGACCGCAA
TCACGCAGCTGACCGGCTGGCCGTCGCTCGGCGTGGTGCCGTGGCTGCCGCAGGCGGCGCTGCTCCCGGCCGAGGACGCG
GTCGATCTCGACCGCGCCCGCGCCTCCGAAGCGACGCGCATCATCGCGGTGCCGATGCTCGCCCGCATCGCCAATTTCGA
CGACCTCGATCCGCTCGGCATGGAGCCCGGCGTCAGACTGGTGTTCGTGCCGCCGGGCCAGCCGATTCCGGCGGAAGCCA
GCGTGGTGATCATCCCCGGCAGCAAATCGACGCTGGGCGACCTCGCCTTCCTGCGCGCGCAGGGCTGGGACATCGACATC
GCCGCCCATGTCCGCCGCGGCGGCCATCTGCTCGGCCTGTGCGGCGGCTATCAGATGCTCGGCCGCTCGATCGCCGATCC
CGACGGCCTCGATGGCGCGCCCGGCACGGTCGACGGCCTCGGACATCTCGATATCACCACACTGATGCAGGGCGACAAAT
CGACCACGCTGGTCGGCGGCACGCATGTCGCCACCGGCGCGCCGGTCGAGGGCTACGAGATCCACCTCGGACGCAGTTCA
GGCCCCGACTGCGCGCGGCCGGTGGTGACGATCGACGGCCGCCCCGACGGCGCGATCTCGCGGGACGGCCGGGTGCAGGG
CACCTATCTGCACGGCCTGTTCGCCAGCGACGCCTTCCGCAAGGCCTGGCTCGCCGGCCTCGGCATCGCCTCCGAGCTTG
CTTACGACGCGCAGGTCGAAGCCGCGCTCGACGCGCTGGCCGATCACCTCGACGCGCATCTCGACATCGACGCGCTGCTG
ACGATCGCGCGGTCGCGTCAGAGCACCAATGCCAGCGCCGCGTAG

Upstream 100 bases:

>100_bases
TACCCCGAGCACCGTGCTCGCGGCACCCCCACCCCGGCCTCCGCTTCGCTCGGCCGACCCTCCCCGCAAGGGGGAGGGGT
TACATGGGCGCCGTGCTCAC

Downstream 100 bases:

>100_bases
ATCAGCGCCTGCAAGACGCAGGCGGCCAGCAGCAGCCGCAGCGCACGGCCGATATCGGCCGGCGTCGCGGCGACGCGGCC
TTCGGCATTGAGCGGCGGAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTPTPALMIQGTCSNAGKSTLVAGLCRALVRRGLRVAPFKPQNMSNNAAVTADGGEIGRAQAVQARAAKLPPSVHMNPVL
LKPETDTGAQVIVQGRRFGRLGAKNFLDKKAALLPHVLDSFARLARDADIVLIEGAGSPAEINLRAGDIANMGFALAANV
PVVIAGDIERGGVIASLAGTHLVLDDDERALVRGYLINKFRGDVRLFDHGLTAITQLTGWPSLGVVPWLPQAALLPAEDA
VDLDRARASEATRIIAVPMLARIANFDDLDPLGMEPGVRLVFVPPGQPIPAEASVVIIPGSKSTLGDLAFLRAQGWDIDI
AAHVRRGGHLLGLCGGYQMLGRSIADPDGLDGAPGTVDGLGHLDITTLMQGDKSTTLVGGTHVATGAPVEGYEIHLGRSS
GPDCARPVVTIDGRPDGAISRDGRVQGTYLHGLFASDAFRKAWLAGLGIASELAYDAQVEAALDALADHLDAHLDIDALL
TIARSRQSTNASAA

Sequences:

>Translated_494_residues
MTPTPALMIQGTCSNAGKSTLVAGLCRALVRRGLRVAPFKPQNMSNNAAVTADGGEIGRAQAVQARAAKLPPSVHMNPVL
LKPETDTGAQVIVQGRRFGRLGAKNFLDKKAALLPHVLDSFARLARDADIVLIEGAGSPAEINLRAGDIANMGFALAANV
PVVIAGDIERGGVIASLAGTHLVLDDDERALVRGYLINKFRGDVRLFDHGLTAITQLTGWPSLGVVPWLPQAALLPAEDA
VDLDRARASEATRIIAVPMLARIANFDDLDPLGMEPGVRLVFVPPGQPIPAEASVVIIPGSKSTLGDLAFLRAQGWDIDI
AAHVRRGGHLLGLCGGYQMLGRSIADPDGLDGAPGTVDGLGHLDITTLMQGDKSTTLVGGTHVATGAPVEGYEIHLGRSS
GPDCARPVVTIDGRPDGAISRDGRVQGTYLHGLFASDAFRKAWLAGLGIASELAYDAQVEAALDALADHLDAHLDIDALL
TIARSRQSTNASAA
>Mature_493_residues
TPTPALMIQGTCSNAGKSTLVAGLCRALVRRGLRVAPFKPQNMSNNAAVTADGGEIGRAQAVQARAAKLPPSVHMNPVLL
KPETDTGAQVIVQGRRFGRLGAKNFLDKKAALLPHVLDSFARLARDADIVLIEGAGSPAEINLRAGDIANMGFALAANVP
VVIAGDIERGGVIASLAGTHLVLDDDERALVRGYLINKFRGDVRLFDHGLTAITQLTGWPSLGVVPWLPQAALLPAEDAV
DLDRARASEATRIIAVPMLARIANFDDLDPLGMEPGVRLVFVPPGQPIPAEASVVIIPGSKSTLGDLAFLRAQGWDIDIA
AHVRRGGHLLGLCGGYQMLGRSIADPDGLDGAPGTVDGLGHLDITTLMQGDKSTTLVGGTHVATGAPVEGYEIHLGRSSG
PDCARPVVTIDGRPDGAISRDGRVQGTYLHGLFASDAFRKAWLAGLGIASELAYDAQVEAALDALADHLDAHLDIDALLT
IARSRQSTNASAA

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 51536; Mature: 51404

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTPALMIQGTCSNAGKSTLVAGLCRALVRRGLRVAPFKPQNMSNNAAVTADGGEIGRA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHH
QAVQARAAKLPPSVHMNPVLLKPETDTGAQVIVQGRRFGRLGAKNFLDKKAALLPHVLDS
HHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHH
FARLARDADIVLIEGAGSPAEINLRAGDIANMGFALAANVPVVIAGDIERGGVIASLAGT
HHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCEEEECCCCCCCEEEEECCC
HLVLDDDERALVRGYLINKFRGDVRLFDHGLTAITQLTGWPSLGVVPWLPQAALLPAEDA
EEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VDLDRARASEATRIIAVPMLARIANFDDLDPLGMEPGVRLVFVPPGQPIPAEASVVIIPG
CCHHHHHHHCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECC
SKSTLGDLAFLRAQGWDIDIAAHVRRGGHLLGLCGGYQMLGRSIADPDGLDGAPGTVDGL
CCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCC
GHLDITTLMQGDKSTTLVGGTHVATGAPVEGYEIHLGRSSGPDCARPVVTIDGRPDGAIS
CCCEEEEEECCCCCEEEECCCEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
RDGRVQGTYLHGLFASDAFRKAWLAGLGIASELAYDAQVEAALDALADHLDAHLDIDALL
CCCEEEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TIARSRQSTNASAA
HHHHHCCCCCCCCC
>Mature Secondary Structure 
TPTPALMIQGTCSNAGKSTLVAGLCRALVRRGLRVAPFKPQNMSNNAAVTADGGEIGRA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHH
QAVQARAAKLPPSVHMNPVLLKPETDTGAQVIVQGRRFGRLGAKNFLDKKAALLPHVLDS
HHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHH
FARLARDADIVLIEGAGSPAEINLRAGDIANMGFALAANVPVVIAGDIERGGVIASLAGT
HHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCEEEECCCCCCCEEEEECCC
HLVLDDDERALVRGYLINKFRGDVRLFDHGLTAITQLTGWPSLGVVPWLPQAALLPAEDA
EEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VDLDRARASEATRIIAVPMLARIANFDDLDPLGMEPGVRLVFVPPGQPIPAEASVVIIPG
CCHHHHHHHCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECC
SKSTLGDLAFLRAQGWDIDIAAHVRRGGHLLGLCGGYQMLGRSIADPDGLDGAPGTVDGL
CCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCC
GHLDITTLMQGDKSTTLVGGTHVATGAPVEGYEIHLGRSSGPDCARPVVTIDGRPDGAIS
CCCEEEEEECCCCCEEEECCCEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
RDGRVQGTYLHGLFASDAFRKAWLAGLGIASELAYDAQVEAALDALADHLDAHLDIDALL
CCCEEEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TIARSRQSTNASAA
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA