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The map label for this gene is cobD [H]

Identifier: 86747861

GI number: 86747861

Start: 824723

End: 825694

Strand: Direct

Name: cobD [H]

Synonym: RPB_0735

Alternate gene names: 86747861

Gene position: 824723-825694 (Clockwise)

Preceding gene: 86747860

Following gene: 86747868

Centisome position: 15.47

GC content: 71.4

Gene sequence:

>972_bases
ATGTTGATCGACTCGAACGCGCTGCTGATCGTGCTGGTGGCGCTGCTGATCGACGCGCTGATCGGCGATCCCGACTGGCT
GTGGAAGCGGATGGCGCATCCGGTGGTGCTGATGGGCGCGCTGATCGGCCGGCTCGATCGCGCGCTCAATCGCGACGCCT
GGTCCGAGGGGCGGCGCAAACTCGCCGGCATTCTGGCCGTGGGGCTGCTGGTCGGGCTCGCCGGCGGCCTCGGCGTGTTG
CTGCAGGCGCCGTTGCGGCAGAGTTGGGGCGGCTGGATCGCGGTCGGCGTGCTGGCGTCGACGCTGATCGCGCAGCGCAG
TCTGTACGAACACGTCGCCCGGGTGCGCGATGCCTTCGCCGAGGGCGGGCTCGCGGCGGCACGCCAGAAGGTGTCGCTGA
TCGTCGGCCGCGATCCGGAGGTACTGGACGAGACCGGGGTCTGCCGCGCGGCGATCGAATCCTGCGCCGAGAATTTCTCC
GACGGCGTGGTGGCTCCGGTGTTCTGGCTGGCGCTGTTCGGGCTGCCGGGGCTGCTGATCTACAAGGTCGTCAACACTGC
GGATTCGATGATCGGCCATCTGACGCCGACGCATCGCTCATTCGGCTGGGCGGCGGCGCGGCTCGACGATGGGCTGAACC
TGATCCCGGCGCGGCTGTCCGGCGTGCTGATCGCCGCACTCGCGCCGGTCGCGGGCGGCGCGATCAAGCGGGCCTTCGCG
GTGATGCGCCGCGACGCGCGCAAGCATCGCTCGCCGAACGCCGGCTGGCCGGAAGCCGCGATGGCCGGCGCGCTCGGCGT
CGCGCTGGCGGGGCCGCGCATCTATGGCGGCCGGGTGGTGGACGATCCGCCGCTCAATGCCGAAGGCCGCGTCGCCGCGA
CGCCGGCCGATATCGGCCGTGCGCTGCGGCTGCTGCTGGCCGCCTGCGTCTTGCAGGCGCTGATCTACGCGGCGCTGGCA
TTGGTGCTCTGA

Upstream 100 bases:

>100_bases
CGCCTCACGATTCCGGAAAATCTCACCGTGTGCGGCGGGCCGATGCTGGCCGATGCGCTCGATCGATTGACCACCGAACT
GACGCGGCTGGAGCGCTGAC

Downstream 100 bases:

>100_bases
CGCGACCGCGCGATCGTCAGCAGCGCGTCGATGTCGAGATGCGCGTCGAGGTGATCGGCCAGCGCGTCGAGCGCGGCTTC
GACCTGCGCGTCGTAAGCAA

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLIDSNALLIVLVALLIDALIGDPDWLWKRMAHPVVLMGALIGRLDRALNRDAWSEGRRKLAGILAVGLLVGLAGGLGVL
LQAPLRQSWGGWIAVGVLASTLIAQRSLYEHVARVRDAFAEGGLAAARQKVSLIVGRDPEVLDETGVCRAAIESCAENFS
DGVVAPVFWLALFGLPGLLIYKVVNTADSMIGHLTPTHRSFGWAAARLDDGLNLIPARLSGVLIAALAPVAGGAIKRAFA
VMRRDARKHRSPNAGWPEAAMAGALGVALAGPRIYGGRVVDDPPLNAEGRVAATPADIGRALRLLLAACVLQALIYAALA
LVL

Sequences:

>Translated_323_residues
MLIDSNALLIVLVALLIDALIGDPDWLWKRMAHPVVLMGALIGRLDRALNRDAWSEGRRKLAGILAVGLLVGLAGGLGVL
LQAPLRQSWGGWIAVGVLASTLIAQRSLYEHVARVRDAFAEGGLAAARQKVSLIVGRDPEVLDETGVCRAAIESCAENFS
DGVVAPVFWLALFGLPGLLIYKVVNTADSMIGHLTPTHRSFGWAAARLDDGLNLIPARLSGVLIAALAPVAGGAIKRAFA
VMRRDARKHRSPNAGWPEAAMAGALGVALAGPRIYGGRVVDDPPLNAEGRVAATPADIGRALRLLLAACVLQALIYAALA
LVL
>Mature_323_residues
MLIDSNALLIVLVALLIDALIGDPDWLWKRMAHPVVLMGALIGRLDRALNRDAWSEGRRKLAGILAVGLLVGLAGGLGVL
LQAPLRQSWGGWIAVGVLASTLIAQRSLYEHVARVRDAFAEGGLAAARQKVSLIVGRDPEVLDETGVCRAAIESCAENFS
DGVVAPVFWLALFGLPGLLIYKVVNTADSMIGHLTPTHRSFGWAAARLDDGLNLIPARLSGVLIAALAPVAGGAIKRAFA
VMRRDARKHRSPNAGWPEAAMAGALGVALAGPRIYGGRVVDDPPLNAEGRVAATPADIGRALRLLLAACVLQALIYAALA
LVL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33925; Mature: 33925

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDSNALLIVLVALLIDALIGDPDWLWKRMAHPVVLMGALIGRLDRALNRDAWSEGRRK
CEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LAGILAVGLLVGLAGGLGVLLQAPLRQSWGGWIAVGVLASTLIAQRSLYEHVARVRDAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGGLAAARQKVSLIVGRDPEVLDETGVCRAAIESCAENFSDGVVAPVFWLALFGLPGLLI
HCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
YKVVNTADSMIGHLTPTHRSFGWAAARLDDGLNLIPARLSGVLIAALAPVAGGAIKRAFA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VMRRDARKHRSPNAGWPEAAMAGALGVALAGPRIYGGRVVDDPPLNAEGRVAATPADIGR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCEECCCCCCCCCCCEECCHHHHHH
ALRLLLAACVLQALIYAALALVL
HHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLIDSNALLIVLVALLIDALIGDPDWLWKRMAHPVVLMGALIGRLDRALNRDAWSEGRRK
CEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LAGILAVGLLVGLAGGLGVLLQAPLRQSWGGWIAVGVLASTLIAQRSLYEHVARVRDAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGGLAAARQKVSLIVGRDPEVLDETGVCRAAIESCAENFSDGVVAPVFWLALFGLPGLLI
HCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
YKVVNTADSMIGHLTPTHRSFGWAAARLDDGLNLIPARLSGVLIAALAPVAGGAIKRAFA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VMRRDARKHRSPNAGWPEAAMAGALGVALAGPRIYGGRVVDDPPLNAEGRVAATPADIGR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCEECCCCCCCCCCCEECCHHHHHH
ALRLLLAACVLQALIYAALALVL
HHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]