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The map label for this gene is phaZ1 [H]

Identifier: 86747837

GI number: 86747837

Start: 796698

End: 797876

Strand: Direct

Name: phaZ1 [H]

Synonym: RPB_0711

Alternate gene names: 86747837

Gene position: 796698-797876 (Clockwise)

Preceding gene: 86747836

Following gene: 86747838

Centisome position: 14.94

GC content: 67.09

Gene sequence:

>1179_bases
GTGTCTCACAGAAATCCCATCGACTTCCTCGCCCATTTGCGAAAGTTCAACGGTCTCAGTGGCTTGCACCCTGCCTCTGG
GGCGGCCGAGGCTGCCAGCCCGCTGATCGAGACGGCGGATTTCGGCGACAACCCCGGCAACCTCCGGATGCTGTCGTTCA
TCCCTGAAGGGCTGACGAAGCGCATCCCGCTGGTGGTCGTGCTGCATGGCTGCACCCAGAATGCGGCCGGTTACGATGTC
GGCGCAGGATGGTCGACGCTAGCGCAGCGCTACGGCTTCGCCCTGCTGATGCCGGAGCAGAAGCGGGACAATAACCCCAA
TCTTTGCTTCAACTGGTTCGTGCCGGACGACATCAAGCGCGGCCACGGCGAAGTCGCGTCGATCTGGCAGATGGTCGAGC
GAATGTCGGCTGCCTATCACATCGATCGGGCGCGAATTTTCGTGACCGGACTATCCGCCGGCGGCGCGATGACCACGGTG
ATGCTGGCGACCTATCCGGATGTGTTCGCCGGCGGTGCGGTGATCGCCGGGCTGCCCTATGGAGTCGCCGGCACCGTACG
GCAGGCGCTGAAGGAGATGCGGCATCCATCGCCGCGTTCCGCGCACGAGCTCGGCGATCTGGTGCGCAAGGCGTCGCCGC
ACAAGGGGCCATGGCCGAAGCTGTCGGTCTGGCACGGCAGCGCCGATCGCACCGTGAACGCCGCCAATGCCGATGCGCTG
GTGAAGCAGTGGCTCGATCTGCACGGTCTGCCGGAAACGCCGATGTCCCAGCGGAGTGTCGTCGGCTTTCCTCACCAGGT
CTGGTGGAACTCCAGCGGCGAGGCGACCGTGGAATCCTACACCATCACCGACATGGCGCACGGCACGCCGATCGGCCGCG
CCAATGACGAGCGCTACGGCGTCGCCGGCGCCTACCTGCTCGAAGCGGGAATTTCGTCATCGTTCCACATCGCCGAATTC
TTCGGGCTCACCGGCCGTATTCACCGGCACAAGGCGGTCGCGCGACCGAGCGCCGAAGCGGACCACGCCGCGGCCGCGGA
CGGCGCGCGAGCGGGAACCTTCAGGCCGCGTGCGGCCGCCGGCGCGCGGGCGGCCCACGAGCCGCCCCGTGACCGCCGCG
GCTTCGATGTCGGCGCGGTGATCACCCGGGCGCTGACTGCGGCCGGCCTGATGAAGTAG

Upstream 100 bases:

>100_bases
TGCAATTCGATCGCAGGAACGGAATTCAGGGTTGCCTATTATCTTGCTGCAGCGCAGCATTATATTCTGGTCAGCTGGCT
GAGGATCAGGAGTTGCTGCC

Downstream 100 bases:

>100_bases
GCGTTCGCTGCTGTCGCTGGTGCTGCAGGCGCAACGCCGGCCTGCCCGGCGCCGACATTTGCGCCTGTCGCGGCGGATGT
TAGGTTGAGCGTTCGCCGCA

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSHRNPIDFLAHLRKFNGLSGLHPASGAAEAASPLIETADFGDNPGNLRMLSFIPEGLTKRIPLVVVLHGCTQNAAGYDV
GAGWSTLAQRYGFALLMPEQKRDNNPNLCFNWFVPDDIKRGHGEVASIWQMVERMSAAYHIDRARIFVTGLSAGGAMTTV
MLATYPDVFAGGAVIAGLPYGVAGTVRQALKEMRHPSPRSAHELGDLVRKASPHKGPWPKLSVWHGSADRTVNAANADAL
VKQWLDLHGLPETPMSQRSVVGFPHQVWWNSSGEATVESYTITDMAHGTPIGRANDERYGVAGAYLLEAGISSSFHIAEF
FGLTGRIHRHKAVARPSAEADHAAAADGARAGTFRPRAAAGARAAHEPPRDRRGFDVGAVITRALTAAGLMK

Sequences:

>Translated_392_residues
MSHRNPIDFLAHLRKFNGLSGLHPASGAAEAASPLIETADFGDNPGNLRMLSFIPEGLTKRIPLVVVLHGCTQNAAGYDV
GAGWSTLAQRYGFALLMPEQKRDNNPNLCFNWFVPDDIKRGHGEVASIWQMVERMSAAYHIDRARIFVTGLSAGGAMTTV
MLATYPDVFAGGAVIAGLPYGVAGTVRQALKEMRHPSPRSAHELGDLVRKASPHKGPWPKLSVWHGSADRTVNAANADAL
VKQWLDLHGLPETPMSQRSVVGFPHQVWWNSSGEATVESYTITDMAHGTPIGRANDERYGVAGAYLLEAGISSSFHIAEF
FGLTGRIHRHKAVARPSAEADHAAAADGARAGTFRPRAAAGARAAHEPPRDRRGFDVGAVITRALTAAGLMK
>Mature_391_residues
SHRNPIDFLAHLRKFNGLSGLHPASGAAEAASPLIETADFGDNPGNLRMLSFIPEGLTKRIPLVVVLHGCTQNAAGYDVG
AGWSTLAQRYGFALLMPEQKRDNNPNLCFNWFVPDDIKRGHGEVASIWQMVERMSAAYHIDRARIFVTGLSAGGAMTTVM
LATYPDVFAGGAVIAGLPYGVAGTVRQALKEMRHPSPRSAHELGDLVRKASPHKGPWPKLSVWHGSADRTVNAANADALV
KQWLDLHGLPETPMSQRSVVGFPHQVWWNSSGEATVESYTITDMAHGTPIGRANDERYGVAGAYLLEAGISSSFHIAEFF
GLTGRIHRHKAVARPSAEADHAAAADGARAGTFRPRAAAGARAAHEPPRDRRGFDVGAVITRALTAAGLMK

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42069; Mature: 41938

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRNPIDFLAHLRKFNGLSGLHPASGAAEAASPLIETADFGDNPGNLRMLSFIPEGLTK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHC
RIPLVVVLHGCTQNAAGYDVGAGWSTLAQRYGFALLMPEQKRDNNPNLCFNWFVPDDIKR
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECHHHCCCCCCEEEEECCCHHHHC
GHGEVASIWQMVERMSAAYHIDRARIFVTGLSAGGAMTTVMLATYPDVFAGGAVIAGLPY
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCHHHCCCEEECCCC
GVAGTVRQALKEMRHPSPRSAHELGDLVRKASPHKGPWPKLSVWHGSADRTVNAANADAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCCHHHH
VKQWLDLHGLPETPMSQRSVVGFPHQVWWNSSGEATVESYTITDMAHGTPIGRANDERYG
HHHHHHHCCCCCCCCCCCCCCCCCHHEEECCCCCEEEEEEEEEHHCCCCCCCCCCCCCCC
VAGAYLLEAGISSSFHIAEFFGLTGRIHRHKAVARPSAEADHAAAADGARAGTFRPRAAA
HHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHC
GARAAHEPPRDRRGFDVGAVITRALTAAGLMK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHRNPIDFLAHLRKFNGLSGLHPASGAAEAASPLIETADFGDNPGNLRMLSFIPEGLTK
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHC
RIPLVVVLHGCTQNAAGYDVGAGWSTLAQRYGFALLMPEQKRDNNPNLCFNWFVPDDIKR
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECHHHCCCCCCEEEEECCCHHHHC
GHGEVASIWQMVERMSAAYHIDRARIFVTGLSAGGAMTTVMLATYPDVFAGGAVIAGLPY
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCHHHCCCEEECCCC
GVAGTVRQALKEMRHPSPRSAHELGDLVRKASPHKGPWPKLSVWHGSADRTVNAANADAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCCHHHH
VKQWLDLHGLPETPMSQRSVVGFPHQVWWNSSGEATVESYTITDMAHGTPIGRANDERYG
HHHHHHHCCCCCCCCCCCCCCCCCHHEEECCCCCEEEEEEEEEHHCCCCCCCCCCCCCCC
VAGAYLLEAGISSSFHIAEFFGLTGRIHRHKAVARPSAEADHAAAADGARAGTFRPRAAA
HHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHC
GARAAHEPPRDRRGFDVGAVITRALTAAGLMK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]