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The map label for this gene is bam [H]

Identifier: 86747817

GI number: 86747817

Start: 776531

End: 778048

Strand: Direct

Name: bam [H]

Synonym: RPB_0691

Alternate gene names: 86747817

Gene position: 776531-778048 (Clockwise)

Preceding gene: 86747812

Following gene: 86747818

Centisome position: 14.56

GC content: 65.09

Gene sequence:

>1518_bases
ATGAATGCGAGAGAGATTTCCAGCGTCGAACTCCTCCGGCACTTCATCCAACAAATCGAAAGCTTCGACGGGCGGATCAA
CGCCGTCGTCAGCCGTGACTTCGAGCGTGCGCTCGACCGCGCAAAGGCGGCGGACGGGACGCGCGCTCGATCGGCCGTCG
GAGAATTGGGCCGCCTTCACGGTCTGCCCATGACCGTCAAGGAGTCATTCGACGTCTCCGGGCTTCCCACCAGTTGGGGC
ACGGTCTCGTATGCGGGCAACGTCGCCAAACGCGATGCCGACGCCGTCGCGCGCCTCGTCGGTGCCGGCGCTGTCGTGTT
CGGCAAGACCAACGTGCCGGAAGGGCTTGCCGACGTGCAAACGTCGAATCCGCTGCACGGCAGGACCAGCAATCCGTGGG
ACCATGCGCGGACTTGCGGGGGCTCGTCCGGCGGTTCGGCGGCGGCGCTGGCGGCCGGCTTCACGGCGGTGGAGCTCGGC
AGCGATCTTGCGGGATCGCTGCGGGTGCCCGCGCATTTCTGCGGTGTCTTCAGCCACAAGCCGTCCTACGGGCTAGTGCC
CCAGAACGGACACTCGATCGATGCCGACGAGTCCCAAACGGATATGACCGTCCTTGGACCGATGGCGCGGTCGGCGTCGG
ATCTGCGCCTGTTGCTCGAGATTCTGGCCGGACCGGGCGGCTTCGATGCGGGCGGCTGGGTGCTGCAGCTGCCCAAGGCG
CGCGGCAACAGATTGAGCGATTTCCGGGTGGCAGTGCTGCCGAACCACGGAGCTTGCGAGGTCGATCAGGGCATTCAAGC
GTCGATCGAAAATCTCGCCCGCGCCCTCCAGCGCAGCGGAGCCGAGGTCGATTTCGACGTGGAGTGGCCCGTCGACCTGG
AACTGTGCTTTCAGGACTTCATGATGATGACGCGCGCGGTCGGCTCCAGGCGTGCATCGCCGGACCTTCTGAAGCGGATG
TCGGACGAGGCGATGACGTTGCGGAGCGACGACCGCAGCTATCGCGCCGCGATCCGGCGTGCGGCGGGGATGAGCCATCA
CGCCTGGTTGTCGCTGCACAGCCGCCGGAGCGAATATCGTGCGGCCTGGCAACGCTTCTTCCGGTCCTACGACGTCGTGC
TTTGCCCGGTTCATGCCTCGCTTGCTTTTGTGCACGATACCGCAGGCCCGCGGGAGAACCGGCTGCTCGACATCAACGGT
CGGCAGCAGGACTACAGTCAATCCCTGTTCTGGATGGCGATCGCTGGGCTCAGCTACCTGCCGTCCACCGTTCGGCCGGT
GTCGCTCGTCGGCGGACTTCCGACCGGCGTCCAGATCATCGGGCCGTATCTGGAAGACATGACCACGCTTCGCTTTGCGG
AGCTTCTGGACGAACTCTGTCCTCACCTGACCTATCCGTTCCAGGCGGCCGGTTCCCGGTCGAGCGTCGACGGACAGGGC
TCGACAGGTCGGCAAGCGCAGACGGACGGATCGGGTATTCAGCCCGGCATTTTGAGAACCGAAGGAGAAAAAGCGTGA

Upstream 100 bases:

>100_bases
AATGCGCCATCAAAAATCGCTCATGATCCGATGATGGATCGAGCCGTCGAGAGAGCGCGTGGGAATCCACTTCAACTCAG
CTTCTGATCTGCTCGCATTG

Downstream 100 bases:

>100_bases
TGGTGAAGTATGCGATTCTCGTTGCGGCTTGTCTGGTCATCGGTGGTGGCGGTCAGTCGAGCGCCCAGGAGATCAAGGTC
GGTCTCACCGCCGATTTCAC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNAREISSVELLRHFIQQIESFDGRINAVVSRDFERALDRAKAADGTRARSAVGELGRLHGLPMTVKESFDVSGLPTSWG
TVSYAGNVAKRDADAVARLVGAGAVVFGKTNVPEGLADVQTSNPLHGRTSNPWDHARTCGGSSGGSAAALAAGFTAVELG
SDLAGSLRVPAHFCGVFSHKPSYGLVPQNGHSIDADESQTDMTVLGPMARSASDLRLLLEILAGPGGFDAGGWVLQLPKA
RGNRLSDFRVAVLPNHGACEVDQGIQASIENLARALQRSGAEVDFDVEWPVDLELCFQDFMMMTRAVGSRRASPDLLKRM
SDEAMTLRSDDRSYRAAIRRAAGMSHHAWLSLHSRRSEYRAAWQRFFRSYDVVLCPVHASLAFVHDTAGPRENRLLDING
RQQDYSQSLFWMAIAGLSYLPSTVRPVSLVGGLPTGVQIIGPYLEDMTTLRFAELLDELCPHLTYPFQAAGSRSSVDGQG
STGRQAQTDGSGIQPGILRTEGEKA

Sequences:

>Translated_505_residues
MNAREISSVELLRHFIQQIESFDGRINAVVSRDFERALDRAKAADGTRARSAVGELGRLHGLPMTVKESFDVSGLPTSWG
TVSYAGNVAKRDADAVARLVGAGAVVFGKTNVPEGLADVQTSNPLHGRTSNPWDHARTCGGSSGGSAAALAAGFTAVELG
SDLAGSLRVPAHFCGVFSHKPSYGLVPQNGHSIDADESQTDMTVLGPMARSASDLRLLLEILAGPGGFDAGGWVLQLPKA
RGNRLSDFRVAVLPNHGACEVDQGIQASIENLARALQRSGAEVDFDVEWPVDLELCFQDFMMMTRAVGSRRASPDLLKRM
SDEAMTLRSDDRSYRAAIRRAAGMSHHAWLSLHSRRSEYRAAWQRFFRSYDVVLCPVHASLAFVHDTAGPRENRLLDING
RQQDYSQSLFWMAIAGLSYLPSTVRPVSLVGGLPTGVQIIGPYLEDMTTLRFAELLDELCPHLTYPFQAAGSRSSVDGQG
STGRQAQTDGSGIQPGILRTEGEKA
>Mature_505_residues
MNAREISSVELLRHFIQQIESFDGRINAVVSRDFERALDRAKAADGTRARSAVGELGRLHGLPMTVKESFDVSGLPTSWG
TVSYAGNVAKRDADAVARLVGAGAVVFGKTNVPEGLADVQTSNPLHGRTSNPWDHARTCGGSSGGSAAALAAGFTAVELG
SDLAGSLRVPAHFCGVFSHKPSYGLVPQNGHSIDADESQTDMTVLGPMARSASDLRLLLEILAGPGGFDAGGWVLQLPKA
RGNRLSDFRVAVLPNHGACEVDQGIQASIENLARALQRSGAEVDFDVEWPVDLELCFQDFMMMTRAVGSRRASPDLLKRM
SDEAMTLRSDDRSYRAAIRRAAGMSHHAWLSLHSRRSEYRAAWQRFFRSYDVVLCPVHASLAFVHDTAGPRENRLLDING
RQQDYSQSLFWMAIAGLSYLPSTVRPVSLVGGLPTGVQIIGPYLEDMTTLRFAELLDELCPHLTYPFQAAGSRSSVDGQG
STGRQAQTDGSGIQPGILRTEGEKA

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=28.3975659229209, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=26.0437375745527, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI166795287, Length=210, Percent_Identity=31.4285714285714, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=484, Percent_Identity=28.099173553719, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556264, Length=291, Percent_Identity=30.5841924398625, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=179, Percent_Identity=35.195530726257, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556278, Length=508, Percent_Identity=25.1968503937008, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=498, Percent_Identity=25.7028112449799, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=207, Percent_Identity=33.8164251207729, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=24.7685185185185, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=183, Percent_Identity=29.5081967213115, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=221, Percent_Identity=29.8642533936652, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=406, Percent_Identity=26.8472906403941, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644968, Length=482, Percent_Identity=28.6307053941909, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45550774, Length=233, Percent_Identity=37.7682403433476, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24648435, Length=233, Percent_Identity=37.7682403433476, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24648437, Length=233, Percent_Identity=37.7682403433476, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21356731, Length=226, Percent_Identity=35.8407079646018, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161078093, Length=232, Percent_Identity=37.5, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=27.5510204081633, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=27.5510204081633, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=27.5510204081633, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=27.5510204081633, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24648441, Length=183, Percent_Identity=39.8907103825137, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648439, Length=183, Percent_Identity=39.8907103825137, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24648113, Length=477, Percent_Identity=28.7211740041929, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 54433; Mature: 54433

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAREISSVELLRHFIQQIESFDGRINAVVSRDFERALDRAKAADGTRARSAVGELGRLH
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLPMTVKESFDVSGLPTSWGTVSYAGNVAKRDADAVARLVGAGAVVFGKTNVPEGLADVQ
CCCEEHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHCC
TSNPLHGRTSNPWDHARTCGGSSGGSAAALAAGFTAVELGSDLAGSLRVPAHFCGVFSHK
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCC
PSYGLVPQNGHSIDADESQTDMTVLGPMARSASDLRLLLEILAGPGGFDAGGWVLQLPKA
CCCCCCCCCCCCCCCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEECCC
RGNRLSDFRVAVLPNHGACEVDQGIQASIENLARALQRSGAEVDFDVEWPVDLELCFQDF
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
MMMTRAVGSRRASPDLLKRMSDEAMTLRSDDRSYRAAIRRAAGMSHHAWLSLHSRRSEYR
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAWQRFFRSYDVVLCPVHASLAFVHDTAGPRENRLLDINGRQQDYSQSLFWMAIAGLSYL
HHHHHHHHCCCEEEEECCHHEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHC
PSTVRPVSLVGGLPTGVQIIGPYLEDMTTLRFAELLDELCPHLTYPFQAAGSRSSVDGQG
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
STGRQAQTDGSGIQPGILRTEGEKA
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAREISSVELLRHFIQQIESFDGRINAVVSRDFERALDRAKAADGTRARSAVGELGRLH
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLPMTVKESFDVSGLPTSWGTVSYAGNVAKRDADAVARLVGAGAVVFGKTNVPEGLADVQ
CCCEEHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHCC
TSNPLHGRTSNPWDHARTCGGSSGGSAAALAAGFTAVELGSDLAGSLRVPAHFCGVFSHK
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCC
PSYGLVPQNGHSIDADESQTDMTVLGPMARSASDLRLLLEILAGPGGFDAGGWVLQLPKA
CCCCCCCCCCCCCCCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEECCC
RGNRLSDFRVAVLPNHGACEVDQGIQASIENLARALQRSGAEVDFDVEWPVDLELCFQDF
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
MMMTRAVGSRRASPDLLKRMSDEAMTLRSDDRSYRAAIRRAAGMSHHAWLSLHSRRSEYR
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAWQRFFRSYDVVLCPVHASLAFVHDTAGPRENRLLDINGRQQDYSQSLFWMAIAGLSYL
HHHHHHHHCCCEEEEECCHHEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHC
PSTVRPVSLVGGLPTGVQIIGPYLEDMTTLRFAELLDELCPHLTYPFQAAGSRSSVDGQG
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
STGRQAQTDGSGIQPGILRTEGEKA
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]