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The map label for this gene is pyrF

Identifier: 86747559

GI number: 86747559

Start: 476700

End: 477407

Strand: Direct

Name: pyrF

Synonym: RPB_0433

Alternate gene names: 86747559

Gene position: 476700-477407 (Clockwise)

Preceding gene: 86747558

Following gene: 86747560

Centisome position: 8.94

GC content: 68.22

Gene sequence:

>708_bases
ATGGCGCAAGCCGACCCCGCTGATCGCGACCGGCTGATCGTCGCATTGGATGTCCCGAGCGTCGACGCCGCCAAGGCGAT
GATCGACAAGCTCGGTGACAGCGTCGGCTTCTACAAGATCGGCTATCAGCTCGCTTATGCCGGCGGGCTCGCGCTGGTGC
CGCAACTGGTCGATGCCGGCAAGAAGGTGTTCGTCGATCTCAAGCTGCACGACATCGGCAACACCGTGGCGCGCGGCGTC
GAGAGCCTGACTGCGCTCGGCGCGACCTTTCTCACCGTGCACGCCTATCCGCAGACCATGCGGGCTGCGGTGGAGGCGCG
CGGAACGTCGGGGATGAAGATTCTCGCCGTCACCGTGCTGACCTCCTACGACGATGCCGACCTCACCGACGCTGGCTACG
CGCTCGGCGTGCGCGATCTCGTCGAGGCGCGCGCGCGGCAGGCGTTGGCGATCGGCGTCGATGGACTGGTGTGCTCGCCT
GAGGAAGCGGCTCACCTGCGCGGCATCATCGGGCCGCGGATGGCGCTGGTGACGCCGGGTATACGGCCGGCGGGCTCGGC
CGCGGGCGATCAGAAGCGGATCATGACGCCGGCGCGGGCGATCGCCGCCGGGGCCAGTCATCTCGTGGTCGGCCGCCCGG
TGATGGAGGCCGCCGATCCAAAGCAGGCGGCGGAGGCGATCGTTGCCGAAATCGCGCAAGCGAAATAA

Upstream 100 bases:

>100_bases
CGAGGCCGACCACCTGAAGCTGCCGGCCGACGTCGCCGCGATGCAGGCGATGGTCCGGCAGTTGATCGATTTCGCCCAAC
AGATGATGAGGTAATTGGTC

Downstream 100 bases:

>100_bases
CTGATCAGGGAGAAGACGATGGCCAAAGGCTACTGGGTCGCACGGATCGACGTGCACGATCTCGACGGCTACAAGCGCGA
CTACGTCGCCCATAACGGCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MAQADPADRDRLIVALDVPSVDAAKAMIDKLGDSVGFYKIGYQLAYAGGLALVPQLVDAGKKVFVDLKLHDIGNTVARGV
ESLTALGATFLTVHAYPQTMRAAVEARGTSGMKILAVTVLTSYDDADLTDAGYALGVRDLVEARARQALAIGVDGLVCSP
EEAAHLRGIIGPRMALVTPGIRPAGSAAGDQKRIMTPARAIAAGASHLVVGRPVMEAADPKQAAEAIVAEIAQAK

Sequences:

>Translated_235_residues
MAQADPADRDRLIVALDVPSVDAAKAMIDKLGDSVGFYKIGYQLAYAGGLALVPQLVDAGKKVFVDLKLHDIGNTVARGV
ESLTALGATFLTVHAYPQTMRAAVEARGTSGMKILAVTVLTSYDDADLTDAGYALGVRDLVEARARQALAIGVDGLVCSP
EEAAHLRGIIGPRMALVTPGIRPAGSAAGDQKRIMTPARAIAAGASHLVVGRPVMEAADPKQAAEAIVAEIAQAK
>Mature_234_residues
AQADPADRDRLIVALDVPSVDAAKAMIDKLGDSVGFYKIGYQLAYAGGLALVPQLVDAGKKVFVDLKLHDIGNTVARGVE
SLTALGATFLTVHAYPQTMRAAVEARGTSGMKILAVTVLTSYDDADLTDAGYALGVRDLVEARARQALAIGVDGLVCSPE
EAAHLRGIIGPRMALVTPGIRPAGSAAGDQKRIMTPARAIAAGASHLVVGRPVMEAADPKQAAEAIVAEIAQAK

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=41.2280701754386, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_RHOP2 (Q2J316)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484055.1
- ProteinModelPortal:   Q2J316
- STRING:   Q2J316
- GeneID:   3909989
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0433
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   RPAL316058:RPB_0433-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24292; Mature: 24161

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 68-68 BINDING 17-17 BINDING 39-39 BINDING 121-121 BINDING 182-182 BINDING 191-191 BINDING 211-211 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQADPADRDRLIVALDVPSVDAAKAMIDKLGDSVGFYKIGYQLAYAGGLALVPQLVDAG
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHEEHHHHCCHHHHHHHHCCC
KKVFVDLKLHDIGNTVARGVESLTALGATFLTVHAYPQTMRAAVEARGTSGMKILAVTVL
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCEEEEEEEE
TSYDDADLTDAGYALGVRDLVEARARQALAIGVDGLVCSPEEAAHLRGIIGPRMALVTPG
ECCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEECCHHHHHHHHHCCCCCEEECCC
IRPAGSAAGDQKRIMTPARAIAAGASHLVVGRPVMEAADPKQAAEAIVAEIAQAK
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQADPADRDRLIVALDVPSVDAAKAMIDKLGDSVGFYKIGYQLAYAGGLALVPQLVDAG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHEEHHHHCCHHHHHHHHCCC
KKVFVDLKLHDIGNTVARGVESLTALGATFLTVHAYPQTMRAAVEARGTSGMKILAVTVL
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCEEEEEEEE
TSYDDADLTDAGYALGVRDLVEARARQALAIGVDGLVCSPEEAAHLRGIIGPRMALVTPG
ECCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEECCHHHHHHHHHCCCCCEEECCC
IRPAGSAAGDQKRIMTPARAIAAGASHLVVGRPVMEAADPKQAAEAIVAEIAQAK
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA