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The map label for this gene is glnB [H]

Identifier: 86747497

GI number: 86747497

Start: 412194

End: 412532

Strand: Direct

Name: glnB [H]

Synonym: RPB_0371

Alternate gene names: 86747497

Gene position: 412194-412532 (Clockwise)

Preceding gene: 86747496

Following gene: 86747498

Centisome position: 7.73

GC content: 65.49

Gene sequence:

>339_bases
ATGAAACTCGTCGTCGCCATCATCAAACCGTTCAAGCTCGACGAGGTGCGTCAGGCGCTGACCGAGATCGGCGTGCACGG
CATGACCGTCACCGAGGTCAAGGGCTACGGCCGTCAGCGCGGCCACACCGAGATCTATCGCGGCGCCGAGTATATCGTGA
ACTTCCTGCCCAAGCTGCGGATCGAGATCGCGGTCGATAGCGCGCTCGCCGACAAGGCGGTCGAGGTCATCACCCGCGGC
GCCCGCACCGGCCAGATCGGCGACGGCAAGATCTTCGTCACCCCGATCGACCACGCGCTGCGGATCCGCACCGGCGAGAC
CGATTCCGACGCGCTCTGA

Upstream 100 bases:

>100_bases
TTATTTTCTGCGCGCATTGCCGCTAAACCCGGCATATACATGACGAAACGCCGCGTCGCGCGGTGCCGATCGCTTTGCGT
GAAGAAATTGGGGCTGCTGC

Downstream 100 bases:

>100_bases
GCCGGCGATCGAGGCAGTTGGAGCGGCGCGGCGGGCGATGCCGAAATGGGCGTCTCGGCATTTCGGAGCATGGCGCGGCG
CCGGATTAGCCACCTGCCGT

Product: nitrogen regulatory protein P-II (GlnB, GlnK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVVAIIKPFKLDEVRQALTEIGVHGMTVTEVKGYGRQRGHTEIYRGAEYIVNFLPKLRIEIAVDSALADKAVEVITRG
ARTGQIGDGKIFVTPIDHALRIRTGETDSDAL

Sequences:

>Translated_112_residues
MKLVVAIIKPFKLDEVRQALTEIGVHGMTVTEVKGYGRQRGHTEIYRGAEYIVNFLPKLRIEIAVDSALADKAVEVITRG
ARTGQIGDGKIFVTPIDHALRIRTGETDSDAL
>Mature_112_residues
MKLVVAIIKPFKLDEVRQALTEIGVHGMTVTEVKGYGRQRGHTEIYRGAEYIVNFLPKLRIEIAVDSALADKAVEVITRG
ARTGQIGDGKIFVTPIDHALRIRTGETDSDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=63.3928571428571, Blast_Score=149, Evalue=6e-38,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=58.9285714285714, Blast_Score=147, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12330; Mature: 12330

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVVAIIKPFKLDEVRQALTEIGVHGMTVTEVKGYGRQRGHTEIYRGAEYIVNFLPKLR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHE
IEIAVDSALADKAVEVITRGARTGQIGDGKIFVTPIDHALRIRTGETDSDAL
EEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKLVVAIIKPFKLDEVRQALTEIGVHGMTVTEVKGYGRQRGHTEIYRGAEYIVNFLPKLR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHE
IEIAVDSALADKAVEVITRGARTGQIGDGKIFVTPIDHALRIRTGETDSDAL
EEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA