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The map label for this gene is czcD [H]

Identifier: 86747454

GI number: 86747454

Start: 372689

End: 373678

Strand: Direct

Name: czcD [H]

Synonym: RPB_0328

Alternate gene names: 86747454

Gene position: 372689-373678 (Clockwise)

Preceding gene: 86747448

Following gene: 86747456

Centisome position: 6.99

GC content: 69.39

Gene sequence:

>990_bases
ATGACCCACGCCCACGACCTTTGCCACGACGACCACGCGCACCCGGACGATTCCGGTGAGATCGCCGGTCGTGACCACGC
GCATGCCCATGGGCACGGCGCGCATAGCCATGCGCCGAAGGATTTCGGCCGCGCCTTTGCGATCGGGATCGGGCTCAACA
TCGGCTTCGTGATCGTCGAGGCGGTGTTCGGCTGGCTCGGCAATTCGATGGCGCTGCTGGCCGACGCCGGCCACAATCTG
TCCGACGTGCTCGGCCTCGCCGTGGCCTGGATCGCGGCGGAACTGTCGAAGCGGCCGCCCTCGGCCCGTTTCACCTACGG
GCTGCGCGGCTCGTCGATCCTCGCCGCGCTGTTCAACGCGGTGTTCCTGCTGCTCGCGGTCGGCGCGATCGGCTGGGAGG
CGATGCTGCGGCTGTTCAATCCCGAGCCGGTCGCCGGCGTCACCATGATGGTGGTGGCCGGCGTCGGCATCGGCATCAAC
GCCGCCACGGCCTGGCTGTTCTTCTCGGGCCGTCACGACGATCTCAACATCCGCGGCGCCTATCTGCACATGATCGCCGA
CGCCGCGGTGTCGGCTGCTGTCGTCGTCGCGGGCGTCGTGATCCTGTTCAGCGGCTGGACCTGGATCGACCCGGCGGTCA
GCCTGCTGGTGGTCGGCGTGATCGTCTGGGGCACCTGGGGCCTGCTGCGCGACTCCACCGCGATGTCGCTCGGCGCGGTG
CCGCGCGGCATCGATCCGGCCGCGGTACGCGCCTTCCTGGCGAGCCGGCCCGGCGTCACGCAGCTGCACGATCTGCACAT
CTGGCCGATGTCGACCACCGAGGTGGCGCTGACCTGCCATCTGGTGATTCCGTCGGGCTCCCCCGGCGACGCCTTCCTGA
TCGAGATCGCCCACGCGCTGCAGCACGATTTCGGCATCGCCCACGCCACCGTGCAGATCGAGACCGACCCGGAAACGCCC
TGCGCGCTGGCGCCGGATCACGTGGTGTAG

Upstream 100 bases:

>100_bases
TCGAACCGCTCTAAGCGATCTTGCGCGTCGCCGCTTCCCGCCATCCCGCCCCCGTGCAATGCTCGCCGAAGCCCCGCCGA
CACTCCCGCGCGAAGCCCAG

Downstream 100 bases:

>100_bases
GCGAGGCACTCGAAGTTTCCCTCCCCCTTGCGGGGAGGGTGGCCGGCCGTAAGGCCGGTCGGGTGGGGGTAGCAACGCAC
GCTGTGCTCGCGGACCCCCA

Product: cation diffusion facilitator family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTHAHDLCHDDHAHPDDSGEIAGRDHAHAHGHGAHSHAPKDFGRAFAIGIGLNIGFVIVEAVFGWLGNSMALLADAGHNL
SDVLGLAVAWIAAELSKRPPSARFTYGLRGSSILAALFNAVFLLLAVGAIGWEAMLRLFNPEPVAGVTMMVVAGVGIGIN
AATAWLFFSGRHDDLNIRGAYLHMIADAAVSAAVVVAGVVILFSGWTWIDPAVSLLVVGVIVWGTWGLLRDSTAMSLGAV
PRGIDPAAVRAFLASRPGVTQLHDLHIWPMSTTEVALTCHLVIPSGSPGDAFLIEIAHALQHDFGIAHATVQIETDPETP
CALAPDHVV

Sequences:

>Translated_329_residues
MTHAHDLCHDDHAHPDDSGEIAGRDHAHAHGHGAHSHAPKDFGRAFAIGIGLNIGFVIVEAVFGWLGNSMALLADAGHNL
SDVLGLAVAWIAAELSKRPPSARFTYGLRGSSILAALFNAVFLLLAVGAIGWEAMLRLFNPEPVAGVTMMVVAGVGIGIN
AATAWLFFSGRHDDLNIRGAYLHMIADAAVSAAVVVAGVVILFSGWTWIDPAVSLLVVGVIVWGTWGLLRDSTAMSLGAV
PRGIDPAAVRAFLASRPGVTQLHDLHIWPMSTTEVALTCHLVIPSGSPGDAFLIEIAHALQHDFGIAHATVQIETDPETP
CALAPDHVV
>Mature_328_residues
THAHDLCHDDHAHPDDSGEIAGRDHAHAHGHGAHSHAPKDFGRAFAIGIGLNIGFVIVEAVFGWLGNSMALLADAGHNLS
DVLGLAVAWIAAELSKRPPSARFTYGLRGSSILAALFNAVFLLLAVGAIGWEAMLRLFNPEPVAGVTMMVVAGVGIGINA
ATAWLFFSGRHDDLNIRGAYLHMIADAAVSAAVVVAGVVILFSGWTWIDPAVSLLVVGVIVWGTWGLLRDSTAMSLGAVP
RGIDPAAVRAFLASRPGVTQLHDLHIWPMSTTEVALTCHLVIPSGSPGDAFLIEIAHALQHDFGIAHATVQIETDPETPC
ALAPDHVV

Specific function: Involved in divalent cation and potassium homeostasis in the cell. Catalyzes the active efflux of zinc, cadmium and cobalt, in exchange for potassium and H(+) ions [H]

COG id: COG1230

COG function: function code P; Co/Zn/Cd efflux system component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily [H]

Homologues:

Organism=Homo sapiens, GI52352805, Length=316, Percent_Identity=26.8987341772152, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI64762489, Length=340, Percent_Identity=24.4117647058824, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI289803013, Length=283, Percent_Identity=25.0883392226148, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI289803009, Length=283, Percent_Identity=25.0883392226148, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI289803007, Length=283, Percent_Identity=25.0883392226148, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI289803003, Length=283, Percent_Identity=25.0883392226148, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21359890, Length=304, Percent_Identity=24.3421052631579, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21361112, Length=290, Percent_Identity=27.9310344827586, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI14210520, Length=220, Percent_Identity=26.8181818181818, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI52351208, Length=165, Percent_Identity=26.0606060606061, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786966, Length=282, Percent_Identity=36.5248226950355, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI193210990, Length=329, Percent_Identity=27.355623100304, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17555794, Length=325, Percent_Identity=28.3076923076923, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17555796, Length=278, Percent_Identity=29.4964028776978, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32564488, Length=292, Percent_Identity=24.6575342465753, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320411, Length=242, Percent_Identity=30.5785123966942, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323899, Length=128, Percent_Identity=35.9375, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324892, Length=124, Percent_Identity=35.4838709677419, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583858, Length=327, Percent_Identity=24.7706422018349, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24585900, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585898, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585896, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921672, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24584409, Length=142, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574430, Length=142, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 34552; Mature: 34421

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAHDLCHDDHAHPDDSGEIAGRDHAHAHGHGAHSHAPKDFGRAFAIGIGLNIGFVIVE
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCHHHHHHH
AVFGWLGNSMALLADAGHNLSDVLGLAVAWIAAELSKRPPSARFTYGLRGSSILAALFNA
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
VFLLLAVGAIGWEAMLRLFNPEPVAGVTMMVVAGVGIGINAATAWLFFSGRHDDLNIRGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHH
YLHMIADAAVSAAVVVAGVVILFSGWTWIDPAVSLLVVGVIVWGTWGLLRDSTAMSLGAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PRGIDPAAVRAFLASRPGVTQLHDLHIWPMSTTEVALTCHLVIPSGSPGDAFLIEIAHAL
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHH
QHDFGIAHATVQIETDPETPCALAPDHVV
HHHCCEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
THAHDLCHDDHAHPDDSGEIAGRDHAHAHGHGAHSHAPKDFGRAFAIGIGLNIGFVIVE
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCHHHHHHH
AVFGWLGNSMALLADAGHNLSDVLGLAVAWIAAELSKRPPSARFTYGLRGSSILAALFNA
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
VFLLLAVGAIGWEAMLRLFNPEPVAGVTMMVVAGVGIGINAATAWLFFSGRHDDLNIRGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHH
YLHMIADAAVSAAVVVAGVVILFSGWTWIDPAVSLLVVGVIVWGTWGLLRDSTAMSLGAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PRGIDPAAVRAFLASRPGVTQLHDLHIWPMSTTEVALTCHLVIPSGSPGDAFLIEIAHAL
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHH
QHDFGIAHATVQIETDPETPCALAPDHVV
HHHCCEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377; 9099864 [H]