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The map label for this gene is acsA [H]

Identifier: 86747445

GI number: 86747445

Start: 362549

End: 364507

Strand: Direct

Name: acsA [H]

Synonym: RPB_0319

Alternate gene names: 86747445

Gene position: 362549-364507 (Clockwise)

Preceding gene: 86747444

Following gene: 86747446

Centisome position: 6.8

GC content: 65.34

Gene sequence:

>1959_bases
ATGTCCGAGAAGATCTACGACGTGCCCTCGGAATGGGCGAGCCGCGCCTTCGTCGACGACGCGAAGTACCGCGAGATGTA
CGCCCGCTCGGTCAATGACCCGAACGGCTTCTGGGCCGACCAAGCCAAACGCATCGACTGGATCAAGCCGCCGCACAAGA
TCGAGAACTGCTCGTTCGCGCCCGGCAACGTCTCGATCAAATGGTTCGAGGACGGCGTCCTCAACGTCGCGCACAACTGC
ATCGACCGCCATCTCGCCACCCGCGGCGACCAGGTCGCGATCATCTGGGAGGGCGACGACCCGTCGCAGTCGCGCCACAT
CACCTATCGCGAGCTGCACGACGAGGTCTGCAAATTCGCCAACATCCTGCGCAGCCGCAATGTCGAGAAGGGCGACCGCG
TCACCATCTATCTGCCGATGATCCCCGAAGCGGCCTTCGCGATGCTGGCCTGCGCGCGGATCGGCGCGATCCATTCGGTG
GTGTTCGCCGGCTTCTCGCCGGACTCGCTGGCCGGCCGCATCAACGACTGCCAGTCCAAGATCGTGATCACCGCCGACGA
AGGCCTGCGCGGCGGCAAGAAGGTGCCGCTGAAGGCCAATGTCGACGCCGCGCTGAAGAAGTGCGACGGCGTCGACTGGG
TGATGGTGGTGAAGCGCACCAGCGCCGCCATCGAGATGGATGACGTTCGCGACTTCTGGTACCACGAGGCCGCCGAGATG
GTGACCACCGAGTGCCCGATCGAGCACATGCACGCGGAAGATCCGCTGTTCATCCTGTACACGTCGGGATCGACCGGCCA
GCCCAAGGGCGTGCTGCACACCTCGGGCGGCTATCTGGTGTTCGCCTCGATGACGCACCAATACGTGTTCGATTATCACG
ACGGCGACATCTACTGGTGCACCGCCGACGTCGGCTGGGTCACCGGCCACAGCTACATCCTCTATGGGCCGCTCGCCAAT
GGCGCCACCACGCTGATGTTCGAAGGCGTGCCGAACTACCCGACCAATTCGCGGTTCTGGGACGTCATCGACAAGCACAA
GGTCAACATCTTCTACACCGCGCCGACCGCGATCCGGGCGCTGATGCAGGCCGGCGACGAGCCGGTGAAAAAGACCTCGC
GCAAATCGCTGCGGCTGCTCGGCTCGGTCGGCGAGCCGATCAACCCGGAAGCCTGGGAGTGGTATCACCGCGTCGTCGGC
GACGACCGCTGCCCGATCGTCGATACCTGGTGGCAGACCGAAACCGGCGGCATCCTGATCACGCCGCTGCCCGGCGCGAC
CAAGCTGAAGCCCGGCTCGGCGACGCGGCCGTTCTTCGGCGTGGTGCCGGAGATCCTCGATCCGGAAGGCGTGGTGCTGG
AAGGCGAATGCACCGGCAATCTGTGCCTGGCGCGGTCCTGGCCGGGGCAGATGCGCACCGTCTATGGCGACCACGAACGG
TTCGAGCAGACCTATTTCTCGGCCTATAAGGGCAAATACTTCACCGGCGACGGCTGCCGCCGCGACGCCGACGGCTATTA
CTGGATCACCGGCCGGGTCGACGACGTCATCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTCGAGTCCTCGCTGG
TGGCGCATCCCAAGGTGTCGGAGGCCGCCGTGGTCGGCTATCCGCACGACATCAAGGGTCAGGGCATCTACGCCTATGTG
ACCCTGATGGCCGGGATCGAGCCGTCGGAGGAGCTGCGCAAGGAGCTGGTGGCCTGGGTCCGCAAGGACATCGGCCCGAT
CGCCTCGCCGGACCTGATCCAGTTCGCGCCCGGCCTGCCGAAGACCCGCTCCGGCAAGATCATGCGCCGCATCCTGCGCA
AGATCGCCGAGGACGAGCCCTCGACGCTCGGCGATACCTCGACGCTGGCCGATCCCGCGGTGGTCGACGATCTCGTCGAG
CATCGCCAGAACAAGCACCACAAGGACAAGGTCGGCTGA

Upstream 100 bases:

>100_bases
CGGAAGGTGCTGACGGATGAGCCCGCCGGGTTTGAAGCGATTTGCGGTCCGTGCACCGACGGCGGGTGCGGCAAGGCCGC
GTATTCGGGTGGAGGAAAGA

Downstream 100 bases:

>100_bases
GCCGCAAGCCGGCTCGCAGGGCGTCACGATCGGCCCCGTCCCTCGCGCGAGGGGCGGGGCTTTTTCGTGCCGTGGCCGGA
TGATCTGACGCCCGTCATCC

Product: acetyl-CoA synthetase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MSEKIYDVPSEWASRAFVDDAKYREMYARSVNDPNGFWADQAKRIDWIKPPHKIENCSFAPGNVSIKWFEDGVLNVAHNC
IDRHLATRGDQVAIIWEGDDPSQSRHITYRELHDEVCKFANILRSRNVEKGDRVTIYLPMIPEAAFAMLACARIGAIHSV
VFAGFSPDSLAGRINDCQSKIVITADEGLRGGKKVPLKANVDAALKKCDGVDWVMVVKRTSAAIEMDDVRDFWYHEAAEM
VTTECPIEHMHAEDPLFILYTSGSTGQPKGVLHTSGGYLVFASMTHQYVFDYHDGDIYWCTADVGWVTGHSYILYGPLAN
GATTLMFEGVPNYPTNSRFWDVIDKHKVNIFYTAPTAIRALMQAGDEPVKKTSRKSLRLLGSVGEPINPEAWEWYHRVVG
DDRCPIVDTWWQTETGGILITPLPGATKLKPGSATRPFFGVVPEILDPEGVVLEGECTGNLCLARSWPGQMRTVYGDHER
FEQTYFSAYKGKYFTGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHPKVSEAAVVGYPHDIKGQGIYAYV
TLMAGIEPSEELRKELVAWVRKDIGPIASPDLIQFAPGLPKTRSGKIMRRILRKIAEDEPSTLGDTSTLADPAVVDDLVE
HRQNKHHKDKVG

Sequences:

>Translated_652_residues
MSEKIYDVPSEWASRAFVDDAKYREMYARSVNDPNGFWADQAKRIDWIKPPHKIENCSFAPGNVSIKWFEDGVLNVAHNC
IDRHLATRGDQVAIIWEGDDPSQSRHITYRELHDEVCKFANILRSRNVEKGDRVTIYLPMIPEAAFAMLACARIGAIHSV
VFAGFSPDSLAGRINDCQSKIVITADEGLRGGKKVPLKANVDAALKKCDGVDWVMVVKRTSAAIEMDDVRDFWYHEAAEM
VTTECPIEHMHAEDPLFILYTSGSTGQPKGVLHTSGGYLVFASMTHQYVFDYHDGDIYWCTADVGWVTGHSYILYGPLAN
GATTLMFEGVPNYPTNSRFWDVIDKHKVNIFYTAPTAIRALMQAGDEPVKKTSRKSLRLLGSVGEPINPEAWEWYHRVVG
DDRCPIVDTWWQTETGGILITPLPGATKLKPGSATRPFFGVVPEILDPEGVVLEGECTGNLCLARSWPGQMRTVYGDHER
FEQTYFSAYKGKYFTGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHPKVSEAAVVGYPHDIKGQGIYAYV
TLMAGIEPSEELRKELVAWVRKDIGPIASPDLIQFAPGLPKTRSGKIMRRILRKIAEDEPSTLGDTSTLADPAVVDDLVE
HRQNKHHKDKVG
>Mature_651_residues
SEKIYDVPSEWASRAFVDDAKYREMYARSVNDPNGFWADQAKRIDWIKPPHKIENCSFAPGNVSIKWFEDGVLNVAHNCI
DRHLATRGDQVAIIWEGDDPSQSRHITYRELHDEVCKFANILRSRNVEKGDRVTIYLPMIPEAAFAMLACARIGAIHSVV
FAGFSPDSLAGRINDCQSKIVITADEGLRGGKKVPLKANVDAALKKCDGVDWVMVVKRTSAAIEMDDVRDFWYHEAAEMV
TTECPIEHMHAEDPLFILYTSGSTGQPKGVLHTSGGYLVFASMTHQYVFDYHDGDIYWCTADVGWVTGHSYILYGPLANG
ATTLMFEGVPNYPTNSRFWDVIDKHKVNIFYTAPTAIRALMQAGDEPVKKTSRKSLRLLGSVGEPINPEAWEWYHRVVGD
DRCPIVDTWWQTETGGILITPLPGATKLKPGSATRPFFGVVPEILDPEGVVLEGECTGNLCLARSWPGQMRTVYGDHERF
EQTYFSAYKGKYFTGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHPKVSEAAVVGYPHDIKGQGIYAYVT
LMAGIEPSEELRKELVAWVRKDIGPIASPDLIQFAPGLPKTRSGKIMRRILRKIAEDEPSTLGDTSTLADPAVVDDLVEH
RQNKHHKDKVG

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI8923896, Length=666, Percent_Identity=55.1051051051051, Blast_Score=724, Evalue=0.0,
Organism=Homo sapiens, GI253970489, Length=679, Percent_Identity=54.0500736377025, Blast_Score=721, Evalue=0.0,
Organism=Homo sapiens, GI28416953, Length=631, Percent_Identity=51.3470681458003, Blast_Score=672, Evalue=0.0,
Organism=Homo sapiens, GI13375727, Length=633, Percent_Identity=37.914691943128, Blast_Score=411, Evalue=1e-114,
Organism=Homo sapiens, GI42544132, Length=540, Percent_Identity=29.8148148148148, Blast_Score=205, Evalue=1e-52,
Organism=Homo sapiens, GI38505220, Length=516, Percent_Identity=31.3953488372093, Blast_Score=202, Evalue=1e-51,
Organism=Homo sapiens, GI31982927, Length=631, Percent_Identity=25.3565768621236, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=28.4313725490196, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI115511026, Length=544, Percent_Identity=28.3088235294118, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI58082049, Length=564, Percent_Identity=26.9503546099291, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI157311624, Length=563, Percent_Identity=26.4653641207815, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI157311622, Length=563, Percent_Identity=26.4653641207815, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI42544134, Length=357, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI195947378, Length=438, Percent_Identity=25.7990867579909, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761345, Length=388, Percent_Identity=27.8350515463918, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI187761343, Length=388, Percent_Identity=27.8350515463918, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI156151445, Length=558, Percent_Identity=24.0143369175627, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=640, Percent_Identity=65.9375, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI1786529, Length=634, Percent_Identity=38.1703470031546, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI221142682, Length=527, Percent_Identity=24.2884250474383, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI145693145, Length=509, Percent_Identity=24.1650294695481, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788107, Length=556, Percent_Identity=24.2805755395683, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=640, Percent_Identity=50.78125, Blast_Score=650, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71982997, Length=640, Percent_Identity=50.78125, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569507, Length=659, Percent_Identity=21.5477996965099, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=524, Percent_Identity=25, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=562, Percent_Identity=24.3772241992883, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=562, Percent_Identity=24.5551601423488, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=518, Percent_Identity=22.7799227799228, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=607, Percent_Identity=22.0757825370675, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=654, Percent_Identity=50.3058103975535, Blast_Score=639, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319264, Length=634, Percent_Identity=49.0536277602524, Blast_Score=608, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6319699, Length=542, Percent_Identity=24.169741697417, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24667955, Length=662, Percent_Identity=54.2296072507553, Blast_Score=712, Evalue=0.0,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=58.5409252669039, Blast_Score=659, Evalue=0.0,
Organism=Drosophila melanogaster, GI24667959, Length=520, Percent_Identity=58.8461538461538, Blast_Score=612, Evalue=1e-175,
Organism=Drosophila melanogaster, GI21355225, Length=650, Percent_Identity=36, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI18859661, Length=535, Percent_Identity=25.7943925233645, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21355181, Length=518, Percent_Identity=22.972972972973, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24581924, Length=528, Percent_Identity=22.3484848484848, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656500, Length=382, Percent_Identity=24.0837696335079, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 72729; Mature: 72598

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00018 EF_HAND_1 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKIYDVPSEWASRAFVDDAKYREMYARSVNDPNGFWADQAKRIDWIKPPHKIENCSFA
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PGNVSIKWFEDGVLNVAHNCIDRHLATRGDQVAIIWEGDDPSQSRHITYRELHDEVCKFA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
NILRSRNVEKGDRVTIYLPMIPEAAFAMLACARIGAIHSVVFAGFSPDSLAGRINDCQSK
HHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCE
IVITADEGLRGGKKVPLKANVDAALKKCDGVDWVMVVKRTSAAIEMDDVRDFWYHEAAEM
EEEEECCCCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHH
VTTECPIEHMHAEDPLFILYTSGSTGQPKGVLHTSGGYLVFASMTHQYVFDYHDGDIYWC
HHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEEEECCEEEEE
TADVGWVTGHSYILYGPLANGATTLMFEGVPNYPTNSRFWDVIDKHKVNIFYTAPTAIRA
ECCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECHHHHHH
LMQAGDEPVKKTSRKSLRLLGSVGEPINPEAWEWYHRVVGDDRCPIVDTWWQTETGGILI
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
TPLPGATKLKPGSATRPFFGVVPEILDPEGVVLEGECTGNLCLARSWPGQMRTVYGDHER
ECCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEECCCCCCEEEEECCCCCHHEECCCHHH
FEQTYFSAYKGKYFTGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHPKVS
HHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCC
EAAVVGYPHDIKGQGIYAYVTLMAGIEPSEELRKELVAWVRKDIGPIASPDLIQFAPGLP
CEEEECCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCC
KTRSGKIMRRILRKIAEDEPSTLGDTSTLADPAVVDDLVEHRQNKHHKDKVG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEKIYDVPSEWASRAFVDDAKYREMYARSVNDPNGFWADQAKRIDWIKPPHKIENCSFA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PGNVSIKWFEDGVLNVAHNCIDRHLATRGDQVAIIWEGDDPSQSRHITYRELHDEVCKFA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
NILRSRNVEKGDRVTIYLPMIPEAAFAMLACARIGAIHSVVFAGFSPDSLAGRINDCQSK
HHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCE
IVITADEGLRGGKKVPLKANVDAALKKCDGVDWVMVVKRTSAAIEMDDVRDFWYHEAAEM
EEEEECCCCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHH
VTTECPIEHMHAEDPLFILYTSGSTGQPKGVLHTSGGYLVFASMTHQYVFDYHDGDIYWC
HHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEEEECCEEEEE
TADVGWVTGHSYILYGPLANGATTLMFEGVPNYPTNSRFWDVIDKHKVNIFYTAPTAIRA
ECCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECHHHHHH
LMQAGDEPVKKTSRKSLRLLGSVGEPINPEAWEWYHRVVGDDRCPIVDTWWQTETGGILI
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
TPLPGATKLKPGSATRPFFGVVPEILDPEGVVLEGECTGNLCLARSWPGQMRTVYGDHER
ECCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEECCCCCCEEEEECCCCCHHEECCCHHH
FEQTYFSAYKGKYFTGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHPKVS
HHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCC
EAAVVGYPHDIKGQGIYAYVTLMAGIEPSEELRKELVAWVRKDIGPIASPDLIQFAPGLP
CEEEECCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCC
KTRSGKIMRRILRKIAEDEPSTLGDTSTLADPAVVDDLVEHRQNKHHKDKVG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA