Click here to switch to the map view.

The map label for this gene is rppH

Identifier: 86747386

GI number: 86747386

Start: 293834

End: 294337

Strand: Direct

Name: rppH

Synonym: RPB_0260

Alternate gene names: 86747386

Gene position: 293834-294337 (Clockwise)

Preceding gene: 86747385

Following gene: 86747388

Centisome position: 5.51

GC content: 64.29

Gene sequence:

>504_bases
ATGGCGCGCTACGAAGACCTGCCCTATCGAACATGCGTCGGCATGATGCTGATCAACCGGGCGGGTCTCGTCTTCATCGG
GCGCCGCGCCGGCGGCATCGAGCATGTCGACGACACCCACGTCTGGCAGATGCCGCAGGGCGGCGTCGATCCCGGCGAGG
ACACTTGGGAAGCCGCCAAGCGCGAGCTGTACGAGGAGACCAACGTCCGTTCGGTGGAGAAGATCGCCGAGGTGCCCGAC
TGGCTGATCTACGACATCCCGCGCACCGTCGCGGGCCGTGCCTGGAAAGGCCGCTATCGCGGCCAGCGCCAGAAATGGTT
CGCGATCCGCTTCACCGGCCAGGACTCCGAGATCGACATCATTGCGCCCCCGGGTCACAAATCCGAATTCACCAGCTGGC
GTTGGGAGCCGATGCTGAATCTGCCCGACCTGATCGTTCCGTTCAAACGCCCGGTCTATGAGCGCGTGGTGAAGGAATTC
TCGCAGCTCGCCATCGCGGTCTGA

Upstream 100 bases:

>100_bases
TCCGGGTTGCTGTTTCGAGGCGTTTTCTTCACGCAACGGCTCGCTTCGCTTGGAACCACACAACAGTCCGCAGGCCGTGG
ACGGACGAGGCACTGACCGA

Downstream 100 bases:

>100_bases
GGATCACCCGGCTTCGCCGTCATTGCGTGCGTCGCGAAGCAATCCAGGGGCGACTGAACGCGTGGTTCTCGGATCGCGGC
TGGATTGCTCGGTCGCTGAC

Product: dinucleoside polyphosphate hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MARYEDLPYRTCVGMMLINRAGLVFIGRRAGGIEHVDDTHVWQMPQGGVDPGEDTWEAAKRELYEETNVRSVEKIAEVPD
WLIYDIPRTVAGRAWKGRYRGQRQKWFAIRFTGQDSEIDIIAPPGHKSEFTSWRWEPMLNLPDLIVPFKRPVYERVVKEF
SQLAIAV

Sequences:

>Translated_167_residues
MARYEDLPYRTCVGMMLINRAGLVFIGRRAGGIEHVDDTHVWQMPQGGVDPGEDTWEAAKRELYEETNVRSVEKIAEVPD
WLIYDIPRTVAGRAWKGRYRGQRQKWFAIRFTGQDSEIDIIAPPGHKSEFTSWRWEPMLNLPDLIVPFKRPVYERVVKEF
SQLAIAV
>Mature_166_residues
ARYEDLPYRTCVGMMLINRAGLVFIGRRAGGIEHVDDTHVWQMPQGGVDPGEDTWEAAKRELYEETNVRSVEKIAEVPDW
LIYDIPRTVAGRAWKGRYRGQRQKWFAIRFTGQDSEIDIIAPPGHKSEFTSWRWEPMLNLPDLIVPFKRPVYERVVKEFS
QLAIAV

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789194, Length=165, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPPH_RHOP2 (Q2J3I9)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483882.1
- ProteinModelPortal:   Q2J3I9
- SMR:   Q2J3I9
- STRING:   Q2J3I9
- GeneID:   3907888
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0260
- eggNOG:   COG0494
- HOGENOM:   HBG302451
- OMA:   AGRAWKG
- ProtClustDB:   PRK00714
- BioCyc:   RPAL316058:RPB_0260-MONOMER
- HAMAP:   MF_00298
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927
- Gene3D:   G3DSA:3.90.79.10

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19390; Mature: 19259

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYEDLPYRTCVGMMLINRAGLVFIGRRAGGIEHVDDTHVWQMPQGGVDPGEDTWEAAK
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
RELYEETNVRSVEKIAEVPDWLIYDIPRTVAGRAWKGRYRGQRQKWFAIRFTGQDSEIDI
HHHHHHCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEE
IAPPGHKSEFTSWRWEPMLNLPDLIVPFKRPVYERVVKEFSQLAIAV
ECCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARYEDLPYRTCVGMMLINRAGLVFIGRRAGGIEHVDDTHVWQMPQGGVDPGEDTWEAAK
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
RELYEETNVRSVEKIAEVPDWLIYDIPRTVAGRAWKGRYRGQRQKWFAIRFTGQDSEIDI
HHHHHHCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEE
IAPPGHKSEFTSWRWEPMLNLPDLIVPFKRPVYERVVKEFSQLAIAV
ECCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA