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The map label for this gene is rlmH

Identifier: 86747382

GI number: 86747382

Start: 289079

End: 289561

Strand: Direct

Name: rlmH

Synonym: RPB_0256

Alternate gene names: 86747382

Gene position: 289079-289561 (Clockwise)

Preceding gene: 86747381

Following gene: 86747383

Centisome position: 5.42

GC content: 67.29

Gene sequence:

>483_bases
ATGCGCCTGACCGTGATTGCCGTCGGCAAACTCAAGCAAGGCCCCGAACGCGAACTCGCCGAGCGCTATCGCGCCCGGTT
CGACGATATCGGCCGCAAGCTCGGCTTTCGCGGCCTGGACATCCATGAAATCAGCGAGAGCCGCGCCCGCGATGCCGCCA
CGCGGATGGCGGAGGAAGCCGCCGCGATCGCGGCGCTGGTTCCGGACGGCGGGGCGCTGGTGACGCTCGACGAACGCGGC
AAGAGCGTCGACAGCGCCGCCTTCGCGGCCCAGCTCGGCCGCTGGCGGGACGAATCGGTGCCCGGTACTATTTTCGTGAT
CGGCGGGGCGGACGGACTTTTGCCTGAATTGCGGCGCAAGGCAAAGCTGTGCCTGTCGTTCGGCGCCGCGACTTGGCCGC
ACCAGATGGTCCGCGTGATGCTGCTGGAGCAGATCTACCGGGCCGCGACGATTTTGGCGGGACATCCTTATCACCGTGCC
TAG

Upstream 100 bases:

>100_bases
TCGAGATCTTGCCCGCGCTGTCCGCGAGCGCGTGTATGCATGTGCGTGTCTCTCAGTCGACGCCTGAGACAGGACGGCGC
AGCGCGGGGAGCGCAAGCCG

Downstream 100 bases:

>100_bases
CGAGCGCCGCAGCGATCAGATGCAACACCACCTTGCCACGGCTGCGAAGGACCAAGCCTCGGGTGCGGGCTGGCCGTCGG
TCGCGCTGCTCTCGGCGAGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRLTVIAVGKLKQGPERELAERYRARFDDIGRKLGFRGLDIHEISESRARDAATRMAEEAAAIAALVPDGGALVTLDERG
KSVDSAAFAAQLGRWRDESVPGTIFVIGGADGLLPELRRKAKLCLSFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRA

Sequences:

>Translated_160_residues
MRLTVIAVGKLKQGPERELAERYRARFDDIGRKLGFRGLDIHEISESRARDAATRMAEEAAAIAALVPDGGALVTLDERG
KSVDSAAFAAQLGRWRDESVPGTIFVIGGADGLLPELRRKAKLCLSFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRA
>Mature_160_residues
MRLTVIAVGKLKQGPERELAERYRARFDDIGRKLGFRGLDIHEISESRARDAATRMAEEAAAIAALVPDGGALVTLDERG
KSVDSAAFAAQLGRWRDESVPGTIFVIGGADGLLPELRRKAKLCLSFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=164, Percent_Identity=33.5365853658537, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_RHOP2 (Q2J3J3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483878.1
- ProteinModelPortal:   Q2J3J3
- SMR:   Q2J3J3
- STRING:   Q2J3J3
- GeneID:   3907884
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0256
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- BioCyc:   RPAL316058:RPB_0256-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17520; Mature: 17520

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTVIAVGKLKQGPERELAERYRARFDDIGRKLGFRGLDIHEISESRARDAATRMAEEA
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AAIAALVPDGGALVTLDERGKSVDSAAFAAQLGRWRDESVPGTIFVIGGADGLLPELRRK
HHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
AKLCLSFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLTVIAVGKLKQGPERELAERYRARFDDIGRKLGFRGLDIHEISESRARDAATRMAEEA
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AAIAALVPDGGALVTLDERGKSVDSAAFAAQLGRWRDESVPGTIFVIGGADGLLPELRRK
HHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
AKLCLSFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA