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The map label for this gene is fixI [H]

Identifier: 86747138

GI number: 86747138

Start: 10656

End: 12851

Strand: Reverse

Name: fixI [H]

Synonym: RPB_0011

Alternate gene names: 86747138

Gene position: 12851-10656 (Counterclockwise)

Preceding gene: 86747139

Following gene: 86747137

Centisome position: 0.24

GC content: 69.54

Gene sequence:

>2196_bases
ATGTTGATGACCCGGGACTTCTCGCACTACGTCAAGGACGTCGATCCGCAGACCGCGCATCTCGACCTCGCGGTCGAGGG
CGTCAGCTGCGCCGGCTGCATGGCCAAGATCGAGCGCGGGCTGTCGGCGATTCCGGACGTGACGCTGGCCCGCGTCAACC
TCACCGACCGCCGCCTCGCGGTGGAGTGGAAGAAGGGCGCGGTGGAGCCGGCGCTGTTCATCGATCGGCTCGCCGAACTA
GGCTACAAGGCCTATCCCTACGAGCCGGTCCGCGCCGAGGTCGAGGAGACCGAGCGCAGCAAGTTCCTGCTGCGCTGCCT
CGGCGTCGCCGCCTTCGCGACCATGAACGTGATGATGCTGTCGGTGCCGGTGTGGGTCGGCGGCGATCTCAGTCCCGAAG
CACGCGATTTCTTCCACTGGCTGTCGGCGCTGGTGGTGCTGCCCTGCGCCGCCTATGCGGGCCAGCCGTTCTTCCAGTCG
GCCTGGCGCGCGCTGAAGGCCAAGAGCGTCAACATGGACGTGCCGATCACGATCGGCGTGGTGCTGGCGCTGGGCATGTC
GCTGGTCGAGACCATCAATCACGCCGAGCACACGTATTTCGATGCGGCGATCATGTTGATCGCCTTCCTGCTGGCGGGCC
GCTTCCTCGACCAGAAGATGCGGCAGAAGACCCGCGCGGTCGCCGGCAATCTGGCCGCGCTGAAGGCCGAGACCGCGACC
AAATTCGTCGGTCCCGACGAAATCAGCGAAGTGCCGATCGCCGCGGTGCGCGCCGGCGACCTCGTGTTGCTGCGCCCGGG
CGAGCGCTGCGCGGTCGACGGCAGCGTGGTCGAGGGCCGCTCCGAGATCGACCAGAGCCTGATCACCGGCGAGACGCTGT
ATGTGAAGGCCGAGCGCGGCACCCAGGTCTATGCCGGCACGCTCAACATCTCCGGCACGCTGCGCGTGCGGGTTTCCGCG
GCGTCGGAAGGCACGCTGCTCGCCGAGATCACCCGGCTGCTCGACCATGCGCTGCAGGCGCGCTCGCGCTACGTTCGGCT
CGCCGATCGTGCCTCGCAGCTCTATGCGCCGGTGGTGCACGCCACCGCGCTGCTCACCGTGATCGGCTGGGTGCTGGCCG
GCGCGAGCTGGCACGATGCCATCGTCATCGGCATCGCCGTGCTGATCATCACCTGCCCCTGCGCGCTCGGTCTGGCGATC
CCCGCGGTGCAGACGGTGGCCTCGGGCGCGATGTTCCGCGCCGGCGTGTTGCTCAATGCCGGCGACGCAATCGAGCGCGC
CGCCGCGGTCGACACCGTCGTGTTCGACAAGACCGGCACGCTGACGCTGCCGGAGCTCGACGTCGTCAACGGCGCCGGCG
TGCCCGACGAGGTCTTCCAGCTCGCCGGGCGCCTCGCGCTCGCCAGCCATCATCCGGTCGCCGCCGCGGTGGCGCGCGCC
GCCCAGGCCAAGGCGCCGCTGCCCGGTGTCACCGAGGAGCCCGGCCGCGGCGTCCGCGCGCTGTTCGAGGGCGAGGAGCT
GCGGCTCGGCAGCCCGGCCTTCTGCGGCGCCGATGCCGAGGCCAACGCCATTCTCAGCGCCGATCCGGAAGTCTCGGTGG
TCGCCTTCAGCCGCGGCGATCGGCACACCGTGTTCGCGGTCCGGCAGTTGCTGCGGCCCGACGCCGCCCGGACGATCGAC
GCGCTGCTCAATCGCGGCATCGCCGTGGAATTGCTGTCCGGCGACCGCGAGCCGGCGGTGCGGCATGCGGCCGACGCGCT
CGGCGTCAGCGCCTGGCGCGCCGGGGTGACGCCGGCCGAGAAGATCGCCCATATCGAGGCCTTGAAGGCGCAGGGGCGCG
TGGTGATGATGGTCGGCGACGGCATGAACGACGCGCCGTCACTCGCCGCCGCGCATGTGTCGATGTCGCCGATCAGCGCC
ACCCATCTCAGCCAGGCGACCGCCGATCTGGTGTTTCTTGGCAAGGAACTTGCACCGGTATTGGCCGCGATCGACTATTC
GCGCAAGGCGATGGCAGTGATGCGGGAGAATCTGACGCTGGCGATCGGCTACAATGTGCTCGCGGTGCCGATTGCGATCG
CGGGCCTCGCCACGCCGCTGATCGCCGCATTGGCGATGTCCGGCTCGTCGATCCTGGTGATGCTGAATGCGATGCGCGCC
CGTCGGGTCCGCCTGGGGGGTGCGTCATGGAAGTGA

Upstream 100 bases:

>100_bases
TGCTGGAGGGCGATCAGAACGGCAACCGCATGTTCTTGTCGAAGAACCGCCTGACGCTGAACTGACGAGCGATCCGGACG
AGGATCAAAAGGCGCGCCGC

Downstream 100 bases:

>100_bases
TGGTGTTTTTGGTGCCGCTGGCGCTGATGCTGGGGCTGCTCGGTCTGGCGGCGTTCCTGTGGTCGCTGAAGAGCGGCCAA
TACGAGGACCTCGACGGCGC

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MLMTRDFSHYVKDVDPQTAHLDLAVEGVSCAGCMAKIERGLSAIPDVTLARVNLTDRRLAVEWKKGAVEPALFIDRLAEL
GYKAYPYEPVRAEVEETERSKFLLRCLGVAAFATMNVMMLSVPVWVGGDLSPEARDFFHWLSALVVLPCAAYAGQPFFQS
AWRALKAKSVNMDVPITIGVVLALGMSLVETINHAEHTYFDAAIMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETAT
KFVGPDEISEVPIAAVRAGDLVLLRPGERCAVDGSVVEGRSEIDQSLITGETLYVKAERGTQVYAGTLNISGTLRVRVSA
ASEGTLLAEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTVIGWVLAGASWHDAIVIGIAVLIITCPCALGLAI
PAVQTVASGAMFRAGVLLNAGDAIERAAAVDTVVFDKTGTLTLPELDVVNGAGVPDEVFQLAGRLALASHHPVAAAVARA
AQAKAPLPGVTEEPGRGVRALFEGEELRLGSPAFCGADAEANAILSADPEVSVVAFSRGDRHTVFAVRQLLRPDAARTID
ALLNRGIAVELLSGDREPAVRHAADALGVSAWRAGVTPAEKIAHIEALKAQGRVVMMVGDGMNDAPSLAAAHVSMSPISA
THLSQATADLVFLGKELAPVLAAIDYSRKAMAVMRENLTLAIGYNVLAVPIAIAGLATPLIAALAMSGSSILVMLNAMRA
RRVRLGGASWK

Sequences:

>Translated_731_residues
MLMTRDFSHYVKDVDPQTAHLDLAVEGVSCAGCMAKIERGLSAIPDVTLARVNLTDRRLAVEWKKGAVEPALFIDRLAEL
GYKAYPYEPVRAEVEETERSKFLLRCLGVAAFATMNVMMLSVPVWVGGDLSPEARDFFHWLSALVVLPCAAYAGQPFFQS
AWRALKAKSVNMDVPITIGVVLALGMSLVETINHAEHTYFDAAIMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETAT
KFVGPDEISEVPIAAVRAGDLVLLRPGERCAVDGSVVEGRSEIDQSLITGETLYVKAERGTQVYAGTLNISGTLRVRVSA
ASEGTLLAEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTVIGWVLAGASWHDAIVIGIAVLIITCPCALGLAI
PAVQTVASGAMFRAGVLLNAGDAIERAAAVDTVVFDKTGTLTLPELDVVNGAGVPDEVFQLAGRLALASHHPVAAAVARA
AQAKAPLPGVTEEPGRGVRALFEGEELRLGSPAFCGADAEANAILSADPEVSVVAFSRGDRHTVFAVRQLLRPDAARTID
ALLNRGIAVELLSGDREPAVRHAADALGVSAWRAGVTPAEKIAHIEALKAQGRVVMMVGDGMNDAPSLAAAHVSMSPISA
THLSQATADLVFLGKELAPVLAAIDYSRKAMAVMRENLTLAIGYNVLAVPIAIAGLATPLIAALAMSGSSILVMLNAMRA
RRVRLGGASWK
>Mature_731_residues
MLMTRDFSHYVKDVDPQTAHLDLAVEGVSCAGCMAKIERGLSAIPDVTLARVNLTDRRLAVEWKKGAVEPALFIDRLAEL
GYKAYPYEPVRAEVEETERSKFLLRCLGVAAFATMNVMMLSVPVWVGGDLSPEARDFFHWLSALVVLPCAAYAGQPFFQS
AWRALKAKSVNMDVPITIGVVLALGMSLVETINHAEHTYFDAAIMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETAT
KFVGPDEISEVPIAAVRAGDLVLLRPGERCAVDGSVVEGRSEIDQSLITGETLYVKAERGTQVYAGTLNISGTLRVRVSA
ASEGTLLAEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTVIGWVLAGASWHDAIVIGIAVLIITCPCALGLAI
PAVQTVASGAMFRAGVLLNAGDAIERAAAVDTVVFDKTGTLTLPELDVVNGAGVPDEVFQLAGRLALASHHPVAAAVARA
AQAKAPLPGVTEEPGRGVRALFEGEELRLGSPAFCGADAEANAILSADPEVSVVAFSRGDRHTVFAVRQLLRPDAARTID
ALLNRGIAVELLSGDREPAVRHAADALGVSAWRAGVTPAEKIAHIEALKAQGRVVMMVGDGMNDAPSLAAAHVSMSPISA
THLSQATADLVFLGKELAPVLAAIDYSRKAMAVMRENLTLAIGYNVLAVPIAIAGLATPLIAALAMSGSSILVMLNAMRA
RRVRLGGASWK

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=789, Percent_Identity=25.7287705956907, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI115529486, Length=844, Percent_Identity=24.8815165876777, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI55743073, Length=561, Percent_Identity=25.8467023172906, Blast_Score=153, Evalue=6e-37,
Organism=Escherichia coli, GI1786691, Length=745, Percent_Identity=31.6778523489933, Blast_Score=277, Evalue=2e-75,
Organism=Escherichia coli, GI1789879, Length=715, Percent_Identity=29.2307692307692, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1786914, Length=525, Percent_Identity=26.6666666666667, Blast_Score=112, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556548, Length=661, Percent_Identity=28.1391830559758, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319772, Length=621, Percent_Identity=29.951690821256, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6320475, Length=620, Percent_Identity=27.258064516129, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6325221, Length=523, Percent_Identity=23.9005736137667, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321430, Length=505, Percent_Identity=24.3564356435644, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221329854, Length=535, Percent_Identity=30.4672897196262, Blast_Score=187, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 77354; Mature: 77354

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTRDFSHYVKDVDPQTAHLDLAVEGVSCAGCMAKIERGLSAIPDVTLARVNLTDRRLA
CCCCCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
VEWKKGAVEPALFIDRLAELGYKAYPYEPVRAEVEETERSKFLLRCLGVAAFATMNVMML
EEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
SVPVWVGGDLSPEARDFFHWLSALVVLPCAAYAGQPFFQSAWRALKAKSVNMDVPITIGV
EECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHH
VLALGMSLVETINHAEHTYFDAAIMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KFVGPDEISEVPIAAVRAGDLVLLRPGERCAVDGSVVEGRSEIDQSLITGETLYVKAERG
HCCCCCHHCCCCEEEEECCCEEEECCCCCEEECCHHHCCHHHHHHHHHCCCEEEEEECCC
TQVYAGTLNISGTLRVRVSAASEGTLLAEITRLLDHALQARSRYVRLADRASQLYAPVVH
CEEEEEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATALLTVIGWVLAGASWHDAIVIGIAVLIITCPCALGLAIPAVQTVASGAMFRAGVLLNA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEC
GDAIERAAAVDTVVFDKTGTLTLPELDVVNGAGVPDEVFQLAGRLALASHHPVAAAVARA
CHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AQAKAPLPGVTEEPGRGVRALFEGEELRLGSPAFCGADAEANAILSADPEVSVVAFSRGD
HHCCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCC
RHTVFAVRQLLRPDAARTIDALLNRGIAVELLSGDREPAVRHAADALGVSAWRAGVTPAE
CHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHH
KIAHIEALKAQGRVVMMVGDGMNDAPSLAAAHVSMSPISATHLSQATADLVFLGKELAPV
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAAIDYSRKAMAVMRENLTLAIGYNVLAVPIAIAGLATPLIAALAMSGSSILVMLNAMRA
HHHHHCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRVRLGGASWK
HHEECCCCCCC
>Mature Secondary Structure
MLMTRDFSHYVKDVDPQTAHLDLAVEGVSCAGCMAKIERGLSAIPDVTLARVNLTDRRLA
CCCCCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
VEWKKGAVEPALFIDRLAELGYKAYPYEPVRAEVEETERSKFLLRCLGVAAFATMNVMML
EEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
SVPVWVGGDLSPEARDFFHWLSALVVLPCAAYAGQPFFQSAWRALKAKSVNMDVPITIGV
EECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHH
VLALGMSLVETINHAEHTYFDAAIMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KFVGPDEISEVPIAAVRAGDLVLLRPGERCAVDGSVVEGRSEIDQSLITGETLYVKAERG
HCCCCCHHCCCCEEEEECCCEEEECCCCCEEECCHHHCCHHHHHHHHHCCCEEEEEECCC
TQVYAGTLNISGTLRVRVSAASEGTLLAEITRLLDHALQARSRYVRLADRASQLYAPVVH
CEEEEEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATALLTVIGWVLAGASWHDAIVIGIAVLIITCPCALGLAIPAVQTVASGAMFRAGVLLNA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEC
GDAIERAAAVDTVVFDKTGTLTLPELDVVNGAGVPDEVFQLAGRLALASHHPVAAAVARA
CHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AQAKAPLPGVTEEPGRGVRALFEGEELRLGSPAFCGADAEANAILSADPEVSVVAFSRGD
HHCCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCC
RHTVFAVRQLLRPDAARTIDALLNRGIAVELLSGDREPAVRHAADALGVSAWRAGVTPAE
CHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHH
KIAHIEALKAQGRVVMMVGDGMNDAPSLAAAHVSMSPISATHLSQATADLVFLGKELAPV
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAAIDYSRKAMAVMRENLTLAIGYNVLAVPIAIAGLATPLIAALAMSGSSILVMLNAMRA
HHHHHCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRVRLGGASWK
HHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]