Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 86747129

GI number: 86747129

Start: 2356

End: 3474

Strand: Direct

Name: dnaN [H]

Synonym: RPB_0002

Alternate gene names: 86747129

Gene position: 2356-3474 (Clockwise)

Preceding gene: 86747128

Following gene: 86747130

Centisome position: 0.04

GC content: 66.13

Gene sequence:

>1119_bases
ATGAAGGTCACGGTCGAACGCGCGCAACTTCTGAAGTCGCTGGGCCACGTCCACCGCGTGGTCGAGCGCCGCAACACCAT
TCCGATTCTCGGCAACGTGCTGGTGCGCGCCGAAAACGCCCAATTGTCGCTGAAGGCGACCGACCTCGACCTCGAAGTCA
CCGAAACGCTGCCGGCCGAGACCGCCACCGCGGGGTCGACCACCGTGCCGGCCCACATGTTCTACGACATCGTCCGCAAG
CTGCCCGACGGCTCGCAGATCGTGCTGGAAAGCGACGGCGACCGTTCGGTGCTGGCGATCCGCGCCGGGCGCTCGCGCTT
CACGCTGCAGACCCTGCCGGAGAGCGACTTCCCGGATCTGGCCGCCGGCGAGATGTCGCATTCGTTTTCGCTCACCGCCT
CCGACGTCAAGCGGCTGATCGACCGCACCCAGTTCGCGATCTCGACCGAAGAGACCCGCTACTACCTCAACGGCATCTAT
CTGCACACCGCCGGCTCGGCCAAGGACGCCAAGCTGCGCGCGGTCGCGACCGACGGCCATCGGCTGGCGCAGGTCGACCT
GACCCAGCCCGCCGGCGCCGCCGGCATGCCCGGCGTGATCGTGCCGCGCAAGACCGTCGGCGAGGTGCAGCGGCTGATCG
AGGACAACGACGCCGAGATCGGCCTCGAACTGTCGGTCGGCAAGATCCGCTTCACCATCGGCAATGTGGTGCTGACCTCG
AAGCTGATCGACGGCACCTTCCCGGATTACGGCCGCGTGATTCCGCAGAACAACGACAAGGAACTGGTGGTCGACAAGAA
GGATTTCGAGGCCGCGGTCGACCGCGTCTCGACGATCTCGAGCGAACGCGGCCGCGCCGTGAAGCTGGCCTTGAGCGCCG
GCAAGCTGGTGCTGTCGGTGACCAACCCGGATTCCGGCAGCGCGACCGAGGAGCTTGAGGTCGAATACGCTTCCGACCCG
CTCGATATCGGCTTCAACTCGCGCTATCTGCTCGACATCGCCGCTCAGATCGAAGGCGAAGTCGCAGTGCTGCGCCTCGC
CGACCCCGGCTCGCCCACCCTGATCCAGGACCGCGACAAGAAAAACGCGCTGTACGTGCTGATGCCGATGCGGGTGTAG

Upstream 100 bases:

>100_bases
GGGTCCCCATCGCCGTGGCGCCGTTTCAGGCCGCCCGGCTTCTCCAGTACCTCCGGCGGCTGCATCACACGATGCGGGTG
CGGATCAGGCGGGTTTTGCA

Downstream 100 bases:

>100_bases
TTCCACCCGCGCCCGACCGGCATCAATTGTCACCGCCCGGCTTGACCGGAGAATCGCCGTAAGCGGCATTTGCCTGACTT
GACCAGACAATGCAGAGCCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAQLSLKATDLDLEVTETLPAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGDRSVLAIRAGRSRFTLQTLPESDFPDLAAGEMSHSFSLTASDVKRLIDRTQFAISTEETRYYLNGIY
LHTAGSAKDAKLRAVATDGHRLAQVDLTQPAGAAGMPGVIVPRKTVGEVQRLIEDNDAEIGLELSVGKIRFTIGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDP
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDKKNALYVLMPMRV

Sequences:

>Translated_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAQLSLKATDLDLEVTETLPAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGDRSVLAIRAGRSRFTLQTLPESDFPDLAAGEMSHSFSLTASDVKRLIDRTQFAISTEETRYYLNGIY
LHTAGSAKDAKLRAVATDGHRLAQVDLTQPAGAAGMPGVIVPRKTVGEVQRLIEDNDAEIGLELSVGKIRFTIGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDP
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDKKNALYVLMPMRV
>Mature_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAQLSLKATDLDLEVTETLPAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGDRSVLAIRAGRSRFTLQTLPESDFPDLAAGEMSHSFSLTASDVKRLIDRTQFAISTEETRYYLNGIY
LHTAGSAKDAKLRAVATDGHRLAQVDLTQPAGAAGMPGVIVPRKTVGEVQRLIEDNDAEIGLELSVGKIRFTIGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDP
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDKKNALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=41.0666666666667, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40401; Mature: 40401

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAQLSLKATDLDLEVTETLPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEEECCCCCC
TATAGSTTVPAHMFYDIVRKLPDGSQIVLESDGDRSVLAIRAGRSRFTLQTLPESDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCCC
AAGEMSHSFSLTASDVKRLIDRTQFAISTEETRYYLNGIYLHTAGSAKDAKLRAVATDGH
CCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLAQVDLTQPAGAAGMPGVIVPRKTVGEVQRLIEDNDAEIGLELSVGKIRFTIGNVVLTS
EEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCEEEEE
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDPLDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDK
ECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEECCCC
KNALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAQLSLKATDLDLEVTETLPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEEECCCCCC
TATAGSTTVPAHMFYDIVRKLPDGSQIVLESDGDRSVLAIRAGRSRFTLQTLPESDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCCC
AAGEMSHSFSLTASDVKRLIDRTQFAISTEETRYYLNGIYLHTAGSAKDAKLRAVATDGH
CCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLAQVDLTQPAGAAGMPGVIVPRKTVGEVQRLIEDNDAEIGLELSVGKIRFTIGNVVLTS
EEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCEEEEE
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDPLDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDK
ECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEECCCC
KNALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]