Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 86610353

GI number: 86610353

Start: 3035334

End: 3036047

Strand: Direct

Name: grpE

Synonym: CYB_2933

Alternate gene names: 86610353

Gene position: 3035334-3036047 (Clockwise)

Preceding gene: 86610352

Following gene: 86610354

Centisome position: 99.63

GC content: 57.42

Gene sequence:

>714_bases
ATGGCCCCAGAAGATACCTATCCTGAACTACAGGAAGAAGAGATCGACGTAGAGGCAGAGCTGGAGAAGCTGATTTTGGA
AGATAGCGAAGCAGAAGCCGGTACCTCCTCCGGAGAAACCGCAGCTGAGCCTTCTCCAGATCCCGGTGAAGCTCTCAAGC
AGCTCCAGCACGAGTTGGAAGTTGTCCGCCAGCAACTCAAAGAGAAAGAAGACGCCTACCTGCGCCTCTACGCCGATTTT
GAAAACTACCGGCGGCGTACCCAGCGGGAAAAAGAAGAGTTCAGCCAAAAAGAACGGCAGAAATTTGTGCTGGAGATCTT
GCCTGTTGTGGACAGCTTTGAGCGGGCTCAGCAGCAGCTGAAGCTGGAAACCGACCGCGAGCGGGAGTTGCACAACAGCT
ACCAGAGCGTCTACCGCCTGCTGGTGGAATGCCTGAAGAAGATGGGCGTCTCCCGCATGAAATCGGTGGGCCAGCCCTTC
GACCCCAACCTGCACGAAGCCATAGCCCGTCAGCCCAGCCCAGACTATCCTGAAGATGTGGTAGCCGTCGAATATCAGCC
GGGTTACAAGTTGGGGGACTTGGTGATCCGTCATGCCATGGTAGCCGTCTCGGCGGGCAGCCCCAGCTCTGAACCATCCC
CCCCAGCCCAAGCCACCATCGAAGCTGGTCCGGAAAACACGCCCGCCTCTCCCCAAAACCCCCAACCTTCCTAG

Upstream 100 bases:

>100_bases
GCAGCGGCGGTTTTGAAACTGCCAAACTGGGGATATCCGAGTCACTGCTCATTGCTAACACTTGCCGACATCGCCCATGA
CTGCCGAAGAGAAGCAACCG

Downstream 100 bases:

>100_bases
CCGAACTTGCTCACTTGAACCTTGACAAACAGAGCCAGAGCAGTCTCAGAAACGCTACAGTAAAGCTACGGAGACGAAAG
CAACGTCTGAAGGTTTCTGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAPEDTYPELQEEEIDVEAELEKLILEDSEAEAGTSSGETAAEPSPDPGEALKQLQHELEVVRQQLKEKEDAYLRLYADF
ENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDRERELHNSYQSVYRLLVECLKKMGVSRMKSVGQPF
DPNLHEAIARQPSPDYPEDVVAVEYQPGYKLGDLVIRHAMVAVSAGSPSSEPSPPAQATIEAGPENTPASPQNPQPS

Sequences:

>Translated_237_residues
MAPEDTYPELQEEEIDVEAELEKLILEDSEAEAGTSSGETAAEPSPDPGEALKQLQHELEVVRQQLKEKEDAYLRLYADF
ENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDRERELHNSYQSVYRLLVECLKKMGVSRMKSVGQPF
DPNLHEAIARQPSPDYPEDVVAVEYQPGYKLGDLVIRHAMVAVSAGSPSSEPSPPAQATIEAGPENTPASPQNPQPS
>Mature_236_residues
APEDTYPELQEEEIDVEAELEKLILEDSEAEAGTSSGETAAEPSPDPGEALKQLQHELEVVRQQLKEKEDAYLRLYADFE
NYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDRERELHNSYQSVYRLLVECLKKMGVSRMKSVGQPFD
PNLHEAIARQPSPDYPEDVVAVEYQPGYKLGDLVIRHAMVAVSAGSPSSEPSPPAQATIEAGPENTPASPQNPQPS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=167, Percent_Identity=34.1317365269461, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788967, Length=135, Percent_Identity=34.0740740740741, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=32.0441988950276, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653432, Length=168, Percent_Identity=30.3571428571429, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SYNJB (Q2JH51)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_479115.1
- ProteinModelPortal:   Q2JH51
- SMR:   Q2JH51
- STRING:   Q2JH51
- GeneID:   3900466
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2933
- TIGR:   CYB_2933
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   DDEGRES
- PhylomeDB:   Q2JH51
- ProtClustDB:   PRK14143
- BioCyc:   SSP321332:CYB_2933-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 26792; Mature: 26661

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPEDTYPELQEEEIDVEAELEKLILEDSEAEAGTSSGETAAEPSPDPGEALKQLQHELE
CCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VVRQQLKEKEDAYLRLYADFENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLETDRERELHNSYQSVYRLLVECLKKMGVSRMKSVGQPFDPNLHEAIARQPSPDYPEDV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
VAVEYQPGYKLGDLVIRHAMVAVSAGSPSSEPSPPAQATIEAGPENTPASPQNPQPS
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
APEDTYPELQEEEIDVEAELEKLILEDSEAEAGTSSGETAAEPSPDPGEALKQLQHELE
CCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VVRQQLKEKEDAYLRLYADFENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLETDRERELHNSYQSVYRLLVECLKKMGVSRMKSVGQPFDPNLHEAIARQPSPDYPEDV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
VAVEYQPGYKLGDLVIRHAMVAVSAGSPSSEPSPPAQATIEAGPENTPASPQNPQPS
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA