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The map label for this gene is yfkN [H]

Identifier: 86610352

GI number: 86610352

Start: 3033492

End: 3035153

Strand: Direct

Name: yfkN [H]

Synonym: CYB_2932

Alternate gene names: 86610352

Gene position: 3033492-3035153 (Clockwise)

Preceding gene: 86610351

Following gene: 86610353

Centisome position: 99.57

GC content: 60.95

Gene sequence:

>1662_bases
ATGCGATTAACAATTCTCCACACTTCGGATCTCCATGCCAATCTACATCCCTGGAACTACTTCACCGGGATCCCTGCGGA
GCACGGTCTAGCCAAGCTGGCCACCCTTATTAAGCGCGTGCGAGCTGCCAGTGAGGATCCCGTCTTACTCATCGACAGCG
GCGATACCATTCAGGGATCCCCCTTGGGCACCTACTATGCGCAAGTGGAGCGCGTCTCCCCTCACCCCTTGGCTCATGCT
CTCAACGCCTTGGGCTACGACGCCTTTACCCCTGGCAACCACGACTTTAACTTTGGGCTGCAAGTTCTCCAGGATTTTAT
CGGCGATCTCCGCTGTCCGGTGCTCTGCGCCAACATCCTCCGCCAGAATGGGGATCCCCTCTTCCAGCCCTACCTGATCC
GGGAATTGGCCGGGGTTCGCGTTGGCTTGCTAGGGCTGACCACCCCCCGCATCGACACCTGGGAGCGACCCGACTACATT
GCGGGGCTGCGCTTTGCCCCCTTGTTGGAGACCGCCCGACACTACCTATCGCTGCTGCGACCTCAGGTGGATGTTTTGAT
CTTGATCCTGCACAGCGGCCCCAACCAATTGCCCCCCCTGCGCACCCCTGAGCATTGGCACAACCCGGATCGCTCCGGCT
GGTGCAGCAACAAAAGCCTGGCAGGGGAAAACGAACTGTTGGCCCTGGCCGAACTGGACGGCATTGACGTCATCCTCTCC
GGCCATACCCACCAGACCATCGCCGGTCTGGGGGGATCCCAAGCTCTGGTAGTTCAGCCCGGCTATTGGGGCAGCCACCT
CTCCCAGGTATCCCTGGAGCTGGAGCGCCAGGGCAGCCGCTGGCAAATTCTCGGGAAAAGCGCACAGCTCCTCTCCGCCG
AAGGGATCCCTCCCGATCCCGAACTCTTGGCCTTGACAGAGCCCTATCATCAAGCCACCCTGCGCTACATCCGTCAGCCC
ATTGGCCAAGCCCGTTCTGCCTATAGCGGCGGTCTGGCAGCCCGTCTGGGGGCCAACCCTCTGGCAGAGCTGCTCCATCA
GGCCCAATTGCAAGCAGTGCGGGAGGCAGGATTCCCCGCAGAGCTCTCCCTGGTCAACATCTGCAGCAGCGCCGGCCTGT
CAGCAGGCCCCATCTCCCTGCAAGATGCCTACAGCCTCACCGTCTACGACAACACCCTCTGCGTTTTGGAGATCACTGGA
GATATTCTGCGGCGCGCCCTAGAACAGACTGCCGGCTATTTTCGCCAGTTGGATCCCAATCACCTTCCCGCCGATCCTGC
TGCCGTCGTGGCCCCCGAAGCCCGCGGCTACACCTTCGACCTCTACCACGGCATCGACTACTGCTTCGATCTCACCCGCC
CCGTAGGAAAGCGCCTGGTGACCTTACAATTCCGCGGACAGCCGGTGGAACCAGCACAGACCTTTCGCCTGGCCATTAAC
CACTACCGAGCCGGCGGCGGCGGCGGTTACAGCATGTTCCAAGAAGCCCAAACCCTGTGGATCTCAGCCGATAGCATCCG
CGACTTTCTGGTCTACTATATTCACACCCACTCCCCCATTCAACCCATCTACAGCCCCAACTGGCGCCTAATCCCCGACT
TGGCCAAGATCTACTTCCCCGATTGCCTGGGCCGGCCACGGGAACATCTGCTGGTCGGCTAG

Upstream 100 bases:

>100_bases
TTAGGCGGCCTCCTCGGCTTTTTGGTGGGCTGTGTACAGGTGCTGTTCTTGCTGGGCTGACGAGTTTGGGTTGGGCTTTG
ACTTTGGCTATGATATGAGA

Downstream 100 bases:

>100_bases
GAAAGAAAAGATATTGACTTCAACTGGCTGAAAGAGAACCCGGTAGAATACGATCAACACAGAGCCTGGTTTGGCTGAGT
GCAGCGGCGGTTTTGAAACT

Product: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRLTILHTSDLHANLHPWNYFTGIPAEHGLAKLATLIKRVRAASEDPVLLIDSGDTIQGSPLGTYYAQVERVSPHPLAHA
LNALGYDAFTPGNHDFNFGLQVLQDFIGDLRCPVLCANILRQNGDPLFQPYLIRELAGVRVGLLGLTTPRIDTWERPDYI
AGLRFAPLLETARHYLSLLRPQVDVLILILHSGPNQLPPLRTPEHWHNPDRSGWCSNKSLAGENELLALAELDGIDVILS
GHTHQTIAGLGGSQALVVQPGYWGSHLSQVSLELERQGSRWQILGKSAQLLSAEGIPPDPELLALTEPYHQATLRYIRQP
IGQARSAYSGGLAARLGANPLAELLHQAQLQAVREAGFPAELSLVNICSSAGLSAGPISLQDAYSLTVYDNTLCVLEITG
DILRRALEQTAGYFRQLDPNHLPADPAAVVAPEARGYTFDLYHGIDYCFDLTRPVGKRLVTLQFRGQPVEPAQTFRLAIN
HYRAGGGGGYSMFQEAQTLWISADSIRDFLVYYIHTHSPIQPIYSPNWRLIPDLAKIYFPDCLGRPREHLLVG

Sequences:

>Translated_553_residues
MRLTILHTSDLHANLHPWNYFTGIPAEHGLAKLATLIKRVRAASEDPVLLIDSGDTIQGSPLGTYYAQVERVSPHPLAHA
LNALGYDAFTPGNHDFNFGLQVLQDFIGDLRCPVLCANILRQNGDPLFQPYLIRELAGVRVGLLGLTTPRIDTWERPDYI
AGLRFAPLLETARHYLSLLRPQVDVLILILHSGPNQLPPLRTPEHWHNPDRSGWCSNKSLAGENELLALAELDGIDVILS
GHTHQTIAGLGGSQALVVQPGYWGSHLSQVSLELERQGSRWQILGKSAQLLSAEGIPPDPELLALTEPYHQATLRYIRQP
IGQARSAYSGGLAARLGANPLAELLHQAQLQAVREAGFPAELSLVNICSSAGLSAGPISLQDAYSLTVYDNTLCVLEITG
DILRRALEQTAGYFRQLDPNHLPADPAAVVAPEARGYTFDLYHGIDYCFDLTRPVGKRLVTLQFRGQPVEPAQTFRLAIN
HYRAGGGGGYSMFQEAQTLWISADSIRDFLVYYIHTHSPIQPIYSPNWRLIPDLAKIYFPDCLGRPREHLLVG
>Mature_553_residues
MRLTILHTSDLHANLHPWNYFTGIPAEHGLAKLATLIKRVRAASEDPVLLIDSGDTIQGSPLGTYYAQVERVSPHPLAHA
LNALGYDAFTPGNHDFNFGLQVLQDFIGDLRCPVLCANILRQNGDPLFQPYLIRELAGVRVGLLGLTTPRIDTWERPDYI
AGLRFAPLLETARHYLSLLRPQVDVLILILHSGPNQLPPLRTPEHWHNPDRSGWCSNKSLAGENELLALAELDGIDVILS
GHTHQTIAGLGGSQALVVQPGYWGSHLSQVSLELERQGSRWQILGKSAQLLSAEGIPPDPELLALTEPYHQATLRYIRQP
IGQARSAYSGGLAARLGANPLAELLHQAQLQAVREAGFPAELSLVNICSSAGLSAGPISLQDAYSLTVYDNTLCVLEITG
DILRRALEQTAGYFRQLDPNHLPADPAAVVAPEARGYTFDLYHGIDYCFDLTRPVGKRLVTLQFRGQPVEPAQTFRLAIN
HYRAGGGGGYSMFQEAQTLWISADSIRDFLVYYIHTHSPIQPIYSPNWRLIPDLAKIYFPDCLGRPREHLLVG

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=561, Percent_Identity=22.2816399286988, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1790658, Length=608, Percent_Identity=28.6184210526316, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786687, Length=571, Percent_Identity=23.1173380035026, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 60953; Mature: 60953

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTILHTSDLHANLHPWNYFTGIPAEHGLAKLATLIKRVRAASEDPVLLIDSGDTIQGS
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PLGTYYAQVERVSPHPLAHALNALGYDAFTPGNHDFNFGLQVLQDFIGDLRCPVLCANIL
CCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RQNGDPLFQPYLIRELAGVRVGLLGLTTPRIDTWERPDYIAGLRFAPLLETARHYLSLLR
HCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHC
PQVDVLILILHSGPNQLPPLRTPEHWHNPDRSGWCSNKSLAGENELLALAELDGIDVILS
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEE
GHTHQTIAGLGGSQALVVQPGYWGSHLSQVSLELERQGSRWQILGKSAQLLSAEGIPPDP
CCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHEECCCCCCCC
ELLALTEPYHQATLRYIRQPIGQARSAYSGGLAARLGANPLAELLHQAQLQAVREAGFPA
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCC
ELSLVNICSSAGLSAGPISLQDAYSLTVYDNTLCVLEITGDILRRALEQTAGYFRQLDPN
HHHHHHHHHHCCCCCCCEEECCCEEEEEECCEEEEEEECHHHHHHHHHHHHHHHHCCCCC
HLPADPAAVVAPEARGYTFDLYHGIDYCFDLTRPVGKRLVTLQFRGQPVEPAQTFRLAIN
CCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHH
HYRAGGGGGYSMFQEAQTLWISADSIRDFLVYYIHTHSPIQPIYSPNWRLIPDLAKIYFP
HHCCCCCCCHHHHHHHHHEEEEHHHHHEEEEEEEECCCCCCCCCCCCCEECHHHHHHHCH
DCLGRPREHLLVG
HHCCCCHHHCCCC
>Mature Secondary Structure
MRLTILHTSDLHANLHPWNYFTGIPAEHGLAKLATLIKRVRAASEDPVLLIDSGDTIQGS
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PLGTYYAQVERVSPHPLAHALNALGYDAFTPGNHDFNFGLQVLQDFIGDLRCPVLCANIL
CCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RQNGDPLFQPYLIRELAGVRVGLLGLTTPRIDTWERPDYIAGLRFAPLLETARHYLSLLR
HCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHC
PQVDVLILILHSGPNQLPPLRTPEHWHNPDRSGWCSNKSLAGENELLALAELDGIDVILS
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEE
GHTHQTIAGLGGSQALVVQPGYWGSHLSQVSLELERQGSRWQILGKSAQLLSAEGIPPDP
CCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHEECCCCCCCC
ELLALTEPYHQATLRYIRQPIGQARSAYSGGLAARLGANPLAELLHQAQLQAVREAGFPA
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCC
ELSLVNICSSAGLSAGPISLQDAYSLTVYDNTLCVLEITGDILRRALEQTAGYFRQLDPN
HHHHHHHHHHCCCCCCCEEECCCEEEEEECCEEEEEEECHHHHHHHHHHHHHHHHCCCCC
HLPADPAAVVAPEARGYTFDLYHGIDYCFDLTRPVGKRLVTLQFRGQPVEPAQTFRLAIN
CCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHH
HYRAGGGGGYSMFQEAQTLWISADSIRDFLVYYIHTHSPIQPIYSPNWRLIPDLAKIYFP
HHCCCCCCCHHHHHHHHHEEEEHHHHHEEEEEEEECCCCCCCCCCCCCEECHHHHHHHCH
DCLGRPREHLLVG
HHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]