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The map label for this gene is cobM [H]

Identifier: 86610134

GI number: 86610134

Start: 2798462

End: 2799262

Strand: Direct

Name: cobM [H]

Synonym: CYB_2708

Alternate gene names: 86610134

Gene position: 2798462-2799262 (Clockwise)

Preceding gene: 86610133

Following gene: 86610135

Centisome position: 91.85

GC content: 62.8

Gene sequence:

>801_bases
ATGCGCGAACCGACGTCGTGGCCTGTCTACATTGTCGGGGCTGGCCCCGGGGATCCGGAGCTGATTACCCTGCGGGGGCA
AAGACTGCTGGCGGAAGCCGATGTAGTGTTCTACACAGGTTCTTTGGTGCCAGAGGGAGTGCTGGCCCATTGTCGCCCTG
GGGTTGAGGCCATTGACACCCGCTCCTTCACGCTGGAAACGCTGCTGCCGCAAATTGTGGAACGGGTCAAGGCGGGGCGG
CGGGTGGTGCGGCTGCAGGATGGGGATCCCTGCCTTTACGGCGCTCTGCACGAGCTGCTGATCCGGCTGGCGGAGGCAGG
GATCCCTTTTGAGATCGTGCCGGGAGTAAGCGCCTTTCAATTGGCAGCCGCCCGTTTGCAGGTGGAGTTGACCATACCCA
AGTTGGTACAGACGATTATCCTCACCCGCGCCAGCGGTCGCACCCAGGTACCGGAAGCAGAAGCCCTGGCCAGTCTGGCA
GCCCACCGCGCCTCTTTGTGTCTATATCTGAGCGCCCGCCACGTAGAACAAGCGCAGGCAGATCTGCTGGCTCACTACCC
CGCCGATACCCCGGTTGCCGTTTGTTATCGCTTGGGTTGGCCGGACGAACAGATCTGGCTGGGATCCCTGGCCGAGATGG
CCGCCCTCACCCGGCAAGCCGGCTTAGAACGCACAGTGCTCTATCTCGTCAGCCCTGCCTTGAAAGGCTTGTCAAGCTCC
AGGTCTGCCCGCTCGCGGCTCTACAGCCCTGACCACACCCACCTCTTCCGTCCCAGAATGGGCCGGCAGCCCCGTTTGTA
A

Upstream 100 bases:

>100_bases
CATGCTTGAGCCTCCAGATAAGTTCCCTTGGAATCCAGTTTGAAAAGACTTTGGAAAAGGGATCTTCAAAGAGGCCCTTG
AAGGGGTAGGAAGCAAGCAA

Downstream 100 bases:

>100_bases
CAGGCTCTTTCAGTATATTGACGGACTCCGGCAGATTGAGCACAATGAGGCAGCAAGATGAGGTCAGAGGCCTGGACTTC
TGGCGAGGGGGTACTCGTTT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MREPTSWPVYIVGAGPGDPELITLRGQRLLAEADVVFYTGSLVPEGVLAHCRPGVEAIDTRSFTLETLLPQIVERVKAGR
RVVRLQDGDPCLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTIPKLVQTIILTRASGRTQVPEAEALASLA
AHRASLCLYLSARHVEQAQADLLAHYPADTPVAVCYRLGWPDEQIWLGSLAEMAALTRQAGLERTVLYLVSPALKGLSSS
RSARSRLYSPDHTHLFRPRMGRQPRL

Sequences:

>Translated_266_residues
MREPTSWPVYIVGAGPGDPELITLRGQRLLAEADVVFYTGSLVPEGVLAHCRPGVEAIDTRSFTLETLLPQIVERVKAGR
RVVRLQDGDPCLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTIPKLVQTIILTRASGRTQVPEAEALASLA
AHRASLCLYLSARHVEQAQADLLAHYPADTPVAVCYRLGWPDEQIWLGSLAEMAALTRQAGLERTVLYLVSPALKGLSSS
RSARSRLYSPDHTHLFRPRMGRQPRL
>Mature_266_residues
MREPTSWPVYIVGAGPGDPELITLRGQRLLAEADVVFYTGSLVPEGVLAHCRPGVEAIDTRSFTLETLLPQIVERVKAGR
RVVRLQDGDPCLYGALHELLIRLAEAGIPFEIVPGVSAFQLAAARLQVELTIPKLVQTIILTRASGRTQVPEAEALASLA
AHRASLCLYLSARHVEQAQADLLAHYPADTPVAVCYRLGWPDEQIWLGSLAEMAALTRQAGLERTVLYLVSPALKGLSSS
RSARSRLYSPDHTHLFRPRMGRQPRL

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=218, Percent_Identity=30.2752293577982, Blast_Score=94, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322922, Length=212, Percent_Identity=24.5283018867925, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 29137; Mature: 29137

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPTSWPVYIVGAGPGDPELITLRGQRLLAEADVVFYTGSLVPEGVLAHCRPGVEAIDT
CCCCCCCCEEEEECCCCCCCEEEECCCCHHHHCCEEEEECCCCCCHHHHHCCCCCHHHCC
RSFTLETLLPQIVERVKAGRRVVRLQDGDPCLYGALHELLIRLAEAGIPFEIVPGVSAFQ
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
LAAARLQVELTIPKLVQTIILTRASGRTQVPEAEALASLAAHRASLCLYLSARHVEQAQA
HHHHHEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLAHYPADTPVAVCYRLGWPDEQIWLGSLAEMAALTRQAGLERTVLYLVSPALKGLSSS
HHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
RSARSRLYSPDHTHLFRPRMGRQPRL
HHHHHHCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MREPTSWPVYIVGAGPGDPELITLRGQRLLAEADVVFYTGSLVPEGVLAHCRPGVEAIDT
CCCCCCCCEEEEECCCCCCCEEEECCCCHHHHCCEEEEECCCCCCHHHHHCCCCCHHHCC
RSFTLETLLPQIVERVKAGRRVVRLQDGDPCLYGALHELLIRLAEAGIPFEIVPGVSAFQ
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
LAAARLQVELTIPKLVQTIILTRASGRTQVPEAEALASLAAHRASLCLYLSARHVEQAQA
HHHHHEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLAHYPADTPVAVCYRLGWPDEQIWLGSLAEMAALTRQAGLERTVLYLVSPALKGLSSS
HHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
RSARSRLYSPDHTHLFRPRMGRQPRL
HHHHHHCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]