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The map label for this gene is pbpF [H]

Identifier: 86609946

GI number: 86609946

Start: 2606114

End: 2608132

Strand: Direct

Name: pbpF [H]

Synonym: CYB_2512

Alternate gene names: 86609946

Gene position: 2606114-2608132 (Clockwise)

Preceding gene: 86609944

Following gene: 86609947

Centisome position: 85.54

GC content: 61.17

Gene sequence:

>2019_bases
TTGCAACACGGACGTGACAGGGTTTTTGACGTGACCTACGGTCGCAGACCATTGGCCCGCCGGGCGAGAAAGCCGGGGTT
GGAGCGTTTCTCCAATTCCCCCTTTTCCCGATCGGGGTTTGACGAGCATGCCGACTATACCCCACCCACCCGCCCCTCCC
GGATTGCCCAGGTTGCCGGTGTGGTGGGCAATCTGTTGCTGGGATCCCTGCTCTTGGGATCCACGGCAACGGCTGCCGGA
TTGGTGGGGCTGGCCATTAGCTTTCGCAACTTGCCGGACGTGCATCAATTGCGGGATTACGTGCCCATTGCCACCACCCA
CATTGTCGATATTCGGGGCGAGCCCATTGCCAGCCTACACGGGGAGGCCAACCGGGAAGTGATCTCCCTTGAACAGGTCT
CGCCGGAGATGAAAAAGGCCTTGCTGGCCATTGAAGACAGCCACTTTTACACCCATCCAGGCATTAATCCGGTCAGCTTG
GGCCGGGCGATCCTAGGCAGTGTGGAGGGGGGTATAGGCTCGGCGGGGGGGGGATCCACCCTGACAATGCAGTTGGTCAA
GAACCTGTTTTTGAGGCCGGAGCGTACCCTCAGCCGTAAAGTCGCCGAGATCGTGTTGGCGGTGCGCCTAGAACAGGTCT
TCAGCAAAGACGAGATCCTGGAGATGTACCTCAACCAGGTGTACTGGGGGCACAATCTCTACGGCATCCAGGCGGCGGCC
CGCAGCTACTTCAACAAGGATGCGTCTGAACTGAACTTGGCTGAAGCGGCGATGTTGGCTGGGATCCTGCCGGCCCCGGA
AACCCTCAGCCCCTTTCGCAACCTGGAGGGAGCCAAACGGCGGCAACACCTGGTGCTGGATCGTCTGGTGGAGCTGAAGT
GGATTACCCCCGAGGAAGCCCAGGCGGCGCGGGAGCAAAAGCTGACCTTAGGCCGCATTACCTCCTTTCAAAGTAACGCC
CCAGCAGTTACCGACGCCATTGAGGCGGAGCTGCGGCGCCGCTATGGGGAACAAGCTCTAATGCAAGGAGGTCTGCGGGT
TCAGTCCACCATCGATCTGCGTCTGCAGCGCCTTGCCCAGCGCATTGTCAATGAAGATGGCCCCCGCATTGGGGCAGCTC
GTCGTGCCGATCAAATGGCTTTGGCGGCAGTGGATCCCCGTACCGGCTATGTGAAAGCCATTGTGGGCGGGATCAACGCT
CAGCGGGGCCAATTTAACCGCGCCACCCAGGCGTTTCGCCAAACGGGATCCACCTTTAAGCCCTATGTGTACTACGCGGC
TCTGGCCAGCGGGCGCTATTCACCCGGCAGCATCTTGGAAGATACCCCCGTCACCTATCCCGGCTCGCCACCCTACAGCC
CGAAAAACTACGACAACACCTTCTATGGCCCCCTCACCTTTGCTCGGGCTTTGGAGCTCTCCCGCAACGTGCCCACGGTC
AAGTTGGCCGATGATGTCGGGATCCGCAACGTCATTGCAGCGGCTCAGGCCACGGGTATTACTGCCGAGATGTTCCCCAA
CTTGGCTACAGCTCTGGGCTCGGCCAGCGTCAGCCCCCTGGAGATGGCGGCCAGCTATGCGGTCTTTGCCAATGGCGGAT
ATCGGGTGGAGCCGACCCTCTTGGCCCAGGTGGTGGATCGCAACGGCCAAATCCTCTTCGAGGCCAGGCCTCAGCCGCAG
CGGGTTTTGGATCCCTGGGCGGTGGCTACGCTCAACCAAATCCTCAAAGGGGTAGTGACCCAAGGCACCGGGACGGCGGC
TCGTTTGGATGACGGGCGACCCGTGGCCGGTAAAACCGGCACCACCTCCGATTTCCGCGACGCTTGGTTCATCGGCTACG
TGCCGCAACTGTCCACCGCCATTTGGATCGGCAACGACAACAACTCTCCCATGCTGCCCGGCACCGCCGGCGGGGCCTTT
GTTGCCCCCACCTGGAAGCGCTTTATGACCGAGGCTCTGCAAGGGATCCCACCTCAGGACTTCCCCAGCCCGCATCAATT
TCCCCCTCCCCGACCATGA

Upstream 100 bases:

>100_bases
TCTTCTCCCCCTAGCCCGGTTGGAGTTGTCGATGGGCAGGGTTGGCCATCCTGCTGCCCCAATAGACGCCGCCCTATAAT
GGGTCTGGCTTGTTGTTGCT

Downstream 100 bases:

>100_bases
CCTACAGCGAAGAGCGGCTGGCGCGTATCGAGCGCACGGTGGAGGCCAATGCCCAGGCCATCGCCCAACTGACCCGACAG
AACCAAGAGGTGCTGCAAAC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MQHGRDRVFDVTYGRRPLARRARKPGLERFSNSPFSRSGFDEHADYTPPTRPSRIAQVAGVVGNLLLGSLLLGSTATAAG
LVGLAISFRNLPDVHQLRDYVPIATTHIVDIRGEPIASLHGEANREVISLEQVSPEMKKALLAIEDSHFYTHPGINPVSL
GRAILGSVEGGIGSAGGGSTLTMQLVKNLFLRPERTLSRKVAEIVLAVRLEQVFSKDEILEMYLNQVYWGHNLYGIQAAA
RSYFNKDASELNLAEAAMLAGILPAPETLSPFRNLEGAKRRQHLVLDRLVELKWITPEEAQAAREQKLTLGRITSFQSNA
PAVTDAIEAELRRRYGEQALMQGGLRVQSTIDLRLQRLAQRIVNEDGPRIGAARRADQMALAAVDPRTGYVKAIVGGINA
QRGQFNRATQAFRQTGSTFKPYVYYAALASGRYSPGSILEDTPVTYPGSPPYSPKNYDNTFYGPLTFARALELSRNVPTV
KLADDVGIRNVIAAAQATGITAEMFPNLATALGSASVSPLEMAASYAVFANGGYRVEPTLLAQVVDRNGQILFEARPQPQ
RVLDPWAVATLNQILKGVVTQGTGTAARLDDGRPVAGKTGTTSDFRDAWFIGYVPQLSTAIWIGNDNNSPMLPGTAGGAF
VAPTWKRFMTEALQGIPPQDFPSPHQFPPPRP

Sequences:

>Translated_672_residues
MQHGRDRVFDVTYGRRPLARRARKPGLERFSNSPFSRSGFDEHADYTPPTRPSRIAQVAGVVGNLLLGSLLLGSTATAAG
LVGLAISFRNLPDVHQLRDYVPIATTHIVDIRGEPIASLHGEANREVISLEQVSPEMKKALLAIEDSHFYTHPGINPVSL
GRAILGSVEGGIGSAGGGSTLTMQLVKNLFLRPERTLSRKVAEIVLAVRLEQVFSKDEILEMYLNQVYWGHNLYGIQAAA
RSYFNKDASELNLAEAAMLAGILPAPETLSPFRNLEGAKRRQHLVLDRLVELKWITPEEAQAAREQKLTLGRITSFQSNA
PAVTDAIEAELRRRYGEQALMQGGLRVQSTIDLRLQRLAQRIVNEDGPRIGAARRADQMALAAVDPRTGYVKAIVGGINA
QRGQFNRATQAFRQTGSTFKPYVYYAALASGRYSPGSILEDTPVTYPGSPPYSPKNYDNTFYGPLTFARALELSRNVPTV
KLADDVGIRNVIAAAQATGITAEMFPNLATALGSASVSPLEMAASYAVFANGGYRVEPTLLAQVVDRNGQILFEARPQPQ
RVLDPWAVATLNQILKGVVTQGTGTAARLDDGRPVAGKTGTTSDFRDAWFIGYVPQLSTAIWIGNDNNSPMLPGTAGGAF
VAPTWKRFMTEALQGIPPQDFPSPHQFPPPRP
>Mature_672_residues
MQHGRDRVFDVTYGRRPLARRARKPGLERFSNSPFSRSGFDEHADYTPPTRPSRIAQVAGVVGNLLLGSLLLGSTATAAG
LVGLAISFRNLPDVHQLRDYVPIATTHIVDIRGEPIASLHGEANREVISLEQVSPEMKKALLAIEDSHFYTHPGINPVSL
GRAILGSVEGGIGSAGGGSTLTMQLVKNLFLRPERTLSRKVAEIVLAVRLEQVFSKDEILEMYLNQVYWGHNLYGIQAAA
RSYFNKDASELNLAEAAMLAGILPAPETLSPFRNLEGAKRRQHLVLDRLVELKWITPEEAQAAREQKLTLGRITSFQSNA
PAVTDAIEAELRRRYGEQALMQGGLRVQSTIDLRLQRLAQRIVNEDGPRIGAARRADQMALAAVDPRTGYVKAIVGGINA
QRGQFNRATQAFRQTGSTFKPYVYYAALASGRYSPGSILEDTPVTYPGSPPYSPKNYDNTFYGPLTFARALELSRNVPTV
KLADDVGIRNVIAAAQATGITAEMFPNLATALGSASVSPLEMAASYAVFANGGYRVEPTLLAQVVDRNGQILFEARPQPQ
RVLDPWAVATLNQILKGVVTQGTGTAARLDDGRPVAGKTGTTSDFRDAWFIGYVPQLSTAIWIGNDNNSPMLPGTAGGAF
VAPTWKRFMTEALQGIPPQDFPSPHQFPPPRP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=540, Percent_Identity=33.1481481481482, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI87082258, Length=304, Percent_Identity=38.4868421052632, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788867, Length=551, Percent_Identity=30.3085299455535, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789601, Length=180, Percent_Identity=37.2222222222222, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 73068; Mature: 73068

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHGRDRVFDVTYGRRPLARRARKPGLERFSNSPFSRSGFDEHADYTPPTRPSRIAQVAG
CCCCCCCEEEECCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVGNLLLGSLLLGSTATAAGLVGLAISFRNLPDVHQLRDYVPIATTHIVDIRGEPIASLH
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEEECCCCHHHHC
GEANREVISLEQVSPEMKKALLAIEDSHFYTHPGINPVSLGRAILGSVEGGIGSAGGGST
CCCCCCEEEHHHCCHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHH
LTMQLVKNLFLRPERTLSRKVAEIVLAVRLEQVFSKDEILEMYLNQVYWGHNLYGIQAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHH
RSYFNKDASELNLAEAAMLAGILPAPETLSPFRNLEGAKRRQHLVLDRLVELKWITPEEA
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
QAAREQKLTLGRITSFQSNAPAVTDAIEAELRRRYGEQALMQGGLRVQSTIDLRLQRLAQ
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
RIVNEDGPRIGAARRADQMALAAVDPRTGYVKAIVGGINAQRGQFNRATQAFRQTGSTFK
HHHCCCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
PYVYYAALASGRYSPGSILEDTPVTYPGSPPYSPKNYDNTFYGPLTFARALELSRNVPTV
CHHHEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEE
KLADDVGIRNVIAAAQATGITAEMFPNLATALGSASVSPLEMAASYAVFANGGYRVEPTL
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCEEECHHH
LAQVVDRNGQILFEARPQPQRVLDPWAVATLNQILKGVVTQGTGTAARLDDGRPVAGKTG
HHHHHCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
TTSDFRDAWFIGYVPQLSTAIWIGNDNNSPMLPGTAGGAFVAPTWKRFMTEALQGIPPQD
CCCCCCCCEEEEEHHCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCC
FPSPHQFPPPRP
CCCCCCCCCCCC
>Mature Secondary Structure
MQHGRDRVFDVTYGRRPLARRARKPGLERFSNSPFSRSGFDEHADYTPPTRPSRIAQVAG
CCCCCCCEEEECCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVGNLLLGSLLLGSTATAAGLVGLAISFRNLPDVHQLRDYVPIATTHIVDIRGEPIASLH
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEEECCCCHHHHC
GEANREVISLEQVSPEMKKALLAIEDSHFYTHPGINPVSLGRAILGSVEGGIGSAGGGST
CCCCCCEEEHHHCCHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHH
LTMQLVKNLFLRPERTLSRKVAEIVLAVRLEQVFSKDEILEMYLNQVYWGHNLYGIQAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHH
RSYFNKDASELNLAEAAMLAGILPAPETLSPFRNLEGAKRRQHLVLDRLVELKWITPEEA
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
QAAREQKLTLGRITSFQSNAPAVTDAIEAELRRRYGEQALMQGGLRVQSTIDLRLQRLAQ
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
RIVNEDGPRIGAARRADQMALAAVDPRTGYVKAIVGGINAQRGQFNRATQAFRQTGSTFK
HHHCCCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
PYVYYAALASGRYSPGSILEDTPVTYPGSPPYSPKNYDNTFYGPLTFARALELSRNVPTV
CHHHEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEE
KLADDVGIRNVIAAAQATGITAEMFPNLATALGSASVSPLEMAASYAVFANGGYRVEPTL
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCEEECHHH
LAQVVDRNGQILFEARPQPQRVLDPWAVATLNQILKGVVTQGTGTAARLDDGRPVAGKTG
HHHHHCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
TTSDFRDAWFIGYVPQLSTAIWIGNDNNSPMLPGTAGGAFVAPTWKRFMTEALQGIPPQD
CCCCCCCCEEEEEHHCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCC
FPSPHQFPPPRP
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]