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The map label for this gene is cobO-1 [C]

Identifier: 86609757

GI number: 86609757

Start: 2427164

End: 2428297

Strand: Direct

Name: cobO-1 [C]

Synonym: CYB_2316

Alternate gene names: 86609757

Gene position: 2427164-2428297 (Clockwise)

Preceding gene: 86609749

Following gene: 86609758

Centisome position: 79.67

GC content: 61.38

Gene sequence:

>1134_bases
ATGGCAGAAATTGGGATTGTGACTGCCCAGCAGTTTGATCGGCGACGGGGCCAGATCCATGTCTACGACGGCGAGGGCAA
AGGCAAGTCCCAGGCGGCCCTAGGGGTTGTGCTGCGTTCCATTGGCTTGGGGATCGAAAAGGCGGCCCCCAGCCGGGTGT
TGCTGGTGCGGTTTCTCAAGGGGCCAGGCCGAACCTATGCTGAGGATGCGGCCATTGCGGCTCTGCAGCGAGGCTTCCCC
CATCTCATCGATCAATTGCGCACCGGGCGGGGAGAGTTTTTCGGCCCAGATGAGATCACCAAGTTTGACCGTCAGGAGGC
CCGCCGCGGTTGGGATGTGGCCAAAGGGGCTTTGGCGTCGGGGTTTTACTCGGTGGTGGTGCTGGATGAGCTCAACCCGG
TGCTGGATCTGGGCCTGTTGCCTGTGGAGGAGGTGGTCAGTACCCTCAAGCAAAAGCCGGAGGCCATGGAGGTGATCATC
ACCGGGCGCGGCGCTCCCAAGGAAATTATCGACCTGGCGGATTTGCACTCGGAAATGCGCAGTGTCCGGCGGGCGACGGA
TCCCCAAAACGGCCAACCCCCTTGCAGCGGCATCGAGATCTACACCGGCAACGGCAAGGGAAAATCCACCAGCGCTCTGG
GGCGGGCTCTGCAGGCGATTGGACGCGGCATCAGTAAGGATCTCTCCCATCGTGTACTGATCATGCAATGGCTGAAGGGG
GGAACGGGCTACACCGAGGATGCAGCCATTCGGGCCCTGCGGGAAAGCTACCCCGATTTGGTGGATCACCAGCGTTGCGG
TCGGGACGCCATTGTCTGGCGGGGCAAACAACAGGAAATCGACTATGTAGAAGCGGAGCGGGGCTGGGAGTTGGCACGGG
CGGCCATCGCTTCTGGCCTGTACAAAACCATCATCTTGGATGAGTTGAACCCGACGGTGGATCTGGAGCTGCTGGACGTG
GAGCCGATTGTCCAGGCTTTGCTGCGCAAGCCGCGGGACACGGAGATCATCATCACGGGGCGGTGCCATGCCCCCTTACC
TTACTTTGAGCTGGCCTCTGTCCACTCGGAAATGGTGAGCCACAAGCACTATGCCGAGCAGGGGGCCGATCTGCGGCGCG
GGGTGGACTATTGA

Upstream 100 bases:

>100_bases
AGTTTCCCCCTCCTGCCCAAGTTCTGGCTAGGCTCCAAGTAGGCTCTAAGATGGGGGTAGAAAAGCGGGATTTTCCAACA
GCAGGATGACGCGGAGGGGC

Downstream 100 bases:

>100_bases
TGGGATCCTTCGCAGCGTTCTCAGCCAATTCTGCTCTCTCTTGGCCTCCAGACCCCAGGACTCTGGCCCAGTTGTTGCTC
ACCCTTTTCCAGACGGAAGT

Product: hypothetical protein

Products: Adenosylcobalamin; Phosphate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAEIGIVTAQQFDRRRGQIHVYDGEGKGKSQAALGVVLRSIGLGIEKAAPSRVLLVRFLKGPGRTYAEDAAIAALQRGFP
HLIDQLRTGRGEFFGPDEITKFDRQEARRGWDVAKGALASGFYSVVVLDELNPVLDLGLLPVEEVVSTLKQKPEAMEVII
TGRGAPKEIIDLADLHSEMRSVRRATDPQNGQPPCSGIEIYTGNGKGKSTSALGRALQAIGRGISKDLSHRVLIMQWLKG
GTGYTEDAAIRALRESYPDLVDHQRCGRDAIVWRGKQQEIDYVEAERGWELARAAIASGLYKTIILDELNPTVDLELLDV
EPIVQALLRKPRDTEIIITGRCHAPLPYFELASVHSEMVSHKHYAEQGADLRRGVDY

Sequences:

>Translated_377_residues
MAEIGIVTAQQFDRRRGQIHVYDGEGKGKSQAALGVVLRSIGLGIEKAAPSRVLLVRFLKGPGRTYAEDAAIAALQRGFP
HLIDQLRTGRGEFFGPDEITKFDRQEARRGWDVAKGALASGFYSVVVLDELNPVLDLGLLPVEEVVSTLKQKPEAMEVII
TGRGAPKEIIDLADLHSEMRSVRRATDPQNGQPPCSGIEIYTGNGKGKSTSALGRALQAIGRGISKDLSHRVLIMQWLKG
GTGYTEDAAIRALRESYPDLVDHQRCGRDAIVWRGKQQEIDYVEAERGWELARAAIASGLYKTIILDELNPTVDLELLDV
EPIVQALLRKPRDTEIIITGRCHAPLPYFELASVHSEMVSHKHYAEQGADLRRGVDY
>Mature_376_residues
AEIGIVTAQQFDRRRGQIHVYDGEGKGKSQAALGVVLRSIGLGIEKAAPSRVLLVRFLKGPGRTYAEDAAIAALQRGFPH
LIDQLRTGRGEFFGPDEITKFDRQEARRGWDVAKGALASGFYSVVVLDELNPVLDLGLLPVEEVVSTLKQKPEAMEVIIT
GRGAPKEIIDLADLHSEMRSVRRATDPQNGQPPCSGIEIYTGNGKGKSTSALGRALQAIGRGISKDLSHRVLIMQWLKGG
TGYTEDAAIRALRESYPDLVDHQRCGRDAIVWRGKQQEIDYVEAERGWELARAAIASGLYKTIILDELNPTVDLELLDVE
PIVQALLRKPRDTEIIITGRCHAPLPYFELASVHSEMVSHKHYAEQGADLRRGVDY

Specific function: Required For Both De Novo Synthesis Of The Corrin Ring For The Assimilation Of Exogenous Corrinoids. Participates In The Adenosylation Of A Variety Of Incomplete And Complete Corrinoids (By Similarity). [C]

COG id: COG2109

COG function: function code H; ATP:corrinoid adenosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787525, Length=176, Percent_Identity=35.7954545454545, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003724 [H]

Pfam domain/function: PF02572 CobA_CobO_BtuR [H]

EC number: 2.5.1.17 [C]

Molecular weight: Translated: 41519; Mature: 41388

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIGIVTAQQFDRRRGQIHVYDGEGKGKSQAALGVVLRSIGLGIEKAAPSRVLLVRFLK
CCCCCEEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHC
GPGRTYAEDAAIAALQRGFPHLIDQLRTGRGEFFGPDEITKFDRQEARRGWDVAKGALAS
CCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
GFYSVVVLDELNPVLDLGLLPVEEVVSTLKQKPEAMEVIITGRGAPKEIIDLADLHSEMR
CCEEEEEECCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
SVRRATDPQNGQPPCSGIEIYTGNGKGKSTSALGRALQAIGRGISKDLSHRVLIMQWLKG
HHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GTGYTEDAAIRALRESYPDLVDHQRCGRDAIVWRGKQQEIDYVEAERGWELARAAIASGL
CCCCCHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YKTIILDELNPTVDLELLDVEPIVQALLRKPRDTEIIITGRCHAPLPYFELASVHSEMVS
HHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
HKHYAEQGADLRRGVDY
HHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
AEIGIVTAQQFDRRRGQIHVYDGEGKGKSQAALGVVLRSIGLGIEKAAPSRVLLVRFLK
CCCCEEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHC
GPGRTYAEDAAIAALQRGFPHLIDQLRTGRGEFFGPDEITKFDRQEARRGWDVAKGALAS
CCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
GFYSVVVLDELNPVLDLGLLPVEEVVSTLKQKPEAMEVIITGRGAPKEIIDLADLHSEMR
CCEEEEEECCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
SVRRATDPQNGQPPCSGIEIYTGNGKGKSTSALGRALQAIGRGISKDLSHRVLIMQWLKG
HHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GTGYTEDAAIRALRESYPDLVDHQRCGRDAIVWRGKQQEIDYVEAERGWELARAAIASGL
CCCCCHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YKTIILDELNPTVDLELLDVEPIVQALLRKPRDTEIIITGRCHAPLPYFELASVHSEMVS
HHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
HKHYAEQGADLRRGVDY
HHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Cob(I)alamin; H2O [C]

Specific reaction: ATP + Cob(I)alamin + H2O <==> Adenosylcobalamin + Phosphate + Diphosphate [C]

General reaction: Adenosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840; 7883709 [H]