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The map label for this gene is pleD [H]

Identifier: 86609720

GI number: 86609720

Start: 2384145

End: 2385239

Strand: Reverse

Name: pleD [H]

Synonym: CYB_2277

Alternate gene names: 86609720

Gene position: 2385239-2384145 (Counterclockwise)

Preceding gene: 86609721

Following gene: 86609717

Centisome position: 78.29

GC content: 59.54

Gene sequence:

>1095_bases
ATGTCGTCCCTTGATCTGGAGGGCTTGGATTCTGTATACCACCCTTCTGGTTCGGCTCTTGGGCCTGCAGAAGAAGCCTT
TTGGAGCTCTGTTCCGGCTCGCACCCCCCGCATTTGGGTTGTTGATGATGACCCAGTGGTTCGTTGCTACATTATCAATG
TTCTCAAAGGCTCTCAGTACTACGTGGAAAGCTGGGAAAGCGCCCGAGCAGCTTGGGATCCCTTGCAAAGCTTGGAAGAG
AGCGAGTGGCCCGACATGGTGATCTGCGACTGGGTCATGCCTGAGATGTCGGGGGTAGAGTTCTGTTGCCGTCTCAAGGG
ATCCGCAGCAGGGCAGTTCGTCTATTTCATTTTGCTCACGTCCCGTTCTGAAGTTGAGGCTCGGGTAGAGGGGCTGGATG
CGGGGGCGGATGAATTCATCACCAAGCCGGTCGATCCCGAGGAGCTGCGGGCGCGGGTGCGGGCAGGGTTACGTCTCCAG
CGGCTCACCCAAGCTTTGGCCCAGGCCAACCGGCAATTGCGAGCCCGCAATGAGTTGTTGGAGTCTTTGTCTTTGACGGA
TCCCTTGACCGGGGCGTTGAACCGGCGAGCTCTGGATCAGGCGCTGCCCCATTTGCTCAAGCAGGTGGGGCCCCGCGGGG
AGGCTCGCTACCGCTATCTCTGCCTGCTGATGATGGATGTGGATCATTTCAAGCAGGTCAACGACAGCTACGGCCACTAC
ATTGGCGACTGTGTGCTGCAGGCCATCGTCGGGCGGCTGCAAAATCAACTGCGGCCCTCCAGCCTGCTCTACCGCTATGG
TGGGGAAGAATTTGTTTGCGTTACGCCCGGGCTTAGCCCCCCCCGCTGCGGTCGCTACGCAGAGTCTCTGCGGCAGGCTA
TCGCCAAACGGCCCATTGACATCTCCCCCAAGCGCAGCCTGCCTGTTACCATCAGCATCGGTGGGATCGTGCTCAGCGAA
GACTACTGCCCAGAACCGGAAACCGCCTTGCAGCAGGCGGATAGCGCGCTCTACCAGGCCAAGCAGGCAGGGCGCAACCG
AGTTCACATCCTCATCCCGACTCCAGGGGTTTATCTGCCCTCTTCCAGCGGATAG

Upstream 100 bases:

>100_bases
TTGTCTGGCCGCTCCCGGCTCTGGTTCCAACTAGCCGTTGGCGCTAGGATTGGGATCTGGCTGCAATGGAGCGCTCCTCT
TTAGACTATGTCATCAGACT

Downstream 100 bases:

>100_bases
CCAGAGGCCACCGCCAAGGCTTCCAGAGAGCGAACGCCTGTGTAGGTGCGGCCATTGATGATCCAGGTGGGATAGCTGGT
GATCCCCGCTTCTGTGCATT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSSLDLEGLDSVYHPSGSALGPAEEAFWSSVPARTPRIWVVDDDPVVRCYIINVLKGSQYYVESWESARAAWDPLQSLEE
SEWPDMVICDWVMPEMSGVEFCCRLKGSAAGQFVYFILLTSRSEVEARVEGLDAGADEFITKPVDPEELRARVRAGLRLQ
RLTQALAQANRQLRARNELLESLSLTDPLTGALNRRALDQALPHLLKQVGPRGEARYRYLCLLMMDVDHFKQVNDSYGHY
IGDCVLQAIVGRLQNQLRPSSLLYRYGGEEFVCVTPGLSPPRCGRYAESLRQAIAKRPIDISPKRSLPVTISIGGIVLSE
DYCPEPETALQQADSALYQAKQAGRNRVHILIPTPGVYLPSSSG

Sequences:

>Translated_364_residues
MSSLDLEGLDSVYHPSGSALGPAEEAFWSSVPARTPRIWVVDDDPVVRCYIINVLKGSQYYVESWESARAAWDPLQSLEE
SEWPDMVICDWVMPEMSGVEFCCRLKGSAAGQFVYFILLTSRSEVEARVEGLDAGADEFITKPVDPEELRARVRAGLRLQ
RLTQALAQANRQLRARNELLESLSLTDPLTGALNRRALDQALPHLLKQVGPRGEARYRYLCLLMMDVDHFKQVNDSYGHY
IGDCVLQAIVGRLQNQLRPSSLLYRYGGEEFVCVTPGLSPPRCGRYAESLRQAIAKRPIDISPKRSLPVTISIGGIVLSE
DYCPEPETALQQADSALYQAKQAGRNRVHILIPTPGVYLPSSSG
>Mature_363_residues
SSLDLEGLDSVYHPSGSALGPAEEAFWSSVPARTPRIWVVDDDPVVRCYIINVLKGSQYYVESWESARAAWDPLQSLEES
EWPDMVICDWVMPEMSGVEFCCRLKGSAAGQFVYFILLTSRSEVEARVEGLDAGADEFITKPVDPEELRARVRAGLRLQR
LTQALAQANRQLRARNELLESLSLTDPLTGALNRRALDQALPHLLKQVGPRGEARYRYLCLLMMDVDHFKQVNDSYGHYI
GDCVLQAIVGRLQNQLRPSSLLYRYGGEEFVCVTPGLSPPRCGRYAESLRQAIAKRPIDISPKRSLPVTISIGGIVLSED
YCPEPETALQQADSALYQAKQAGRNRVHILIPTPGVYLPSSSG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=37.8048780487805, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693134, Length=199, Percent_Identity=32.6633165829146, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=36.2573099415205, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=183, Percent_Identity=28.9617486338798, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=30.8510638297872, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=35.1515151515151, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=36.8852459016393, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788956, Length=213, Percent_Identity=30.9859154929577, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=33.7349397590361, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=29.2553191489362, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=30.2857142857143, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=35.632183908046, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=31.4606741573034, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081921, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=33.6065573770492, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=128, Percent_Identity=32.03125, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=33.0645161290323, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=28.0487804878049, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40482; Mature: 40350

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLDLEGLDSVYHPSGSALGPAEEAFWSSVPARTPRIWVVDDDPVVRCYIINVLKGSQY
CCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEHHCCCHH
YVESWESARAAWDPLQSLEESEWPDMVICDWVMPEMSGVEFCCRLKGSAAGQFVYFILLT
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHCCCCCCHHHHHHCCCCCCCCEEEEEEEE
SRSEVEARVEGLDAGADEFITKPVDPEELRARVRAGLRLQRLTQALAQANRQLRARNELL
CCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ESLSLTDPLTGALNRRALDQALPHLLKQVGPRGEARYRYLCLLMMDVDHFKQVNDSYGHY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGDCVLQAIVGRLQNQLRPSSLLYRYGGEEFVCVTPGLSPPRCGRYAESLRQAIAKRPID
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
ISPKRSLPVTISIGGIVLSEDYCPEPETALQQADSALYQAKQAGRNRVHILIPTPGVYLP
CCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECC
SSSG
CCCC
>Mature Secondary Structure 
SSLDLEGLDSVYHPSGSALGPAEEAFWSSVPARTPRIWVVDDDPVVRCYIINVLKGSQY
CCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEHHCCCHH
YVESWESARAAWDPLQSLEESEWPDMVICDWVMPEMSGVEFCCRLKGSAAGQFVYFILLT
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHCCCCCCHHHHHHCCCCCCCCEEEEEEEE
SRSEVEARVEGLDAGADEFITKPVDPEELRARVRAGLRLQRLTQALAQANRQLRARNELL
CCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ESLSLTDPLTGALNRRALDQALPHLLKQVGPRGEARYRYLCLLMMDVDHFKQVNDSYGHY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGDCVLQAIVGRLQNQLRPSSLLYRYGGEEFVCVTPGLSPPRCGRYAESLRQAIAKRPID
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
ISPKRSLPVTISIGGIVLSEDYCPEPETALQQADSALYQAKQAGRNRVHILIPTPGVYLP
CCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECC
SSSG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA