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The map label for this gene is metG [H]

Identifier: 86609674

GI number: 86609674

Start: 2335086

End: 2336642

Strand: Reverse

Name: metG [H]

Synonym: CYB_2229

Alternate gene names: 86609674

Gene position: 2336642-2335086 (Counterclockwise)

Preceding gene: 86609680

Following gene: 86609669

Centisome position: 76.69

GC content: 59.86

Gene sequence:

>1557_bases
ATGCCGGATTCCACCTTTGTCATCACCACCCCCATCTACTACGTCAACGCTGCCCCCCATATTGGCAGCGCCTACACCAC
CCTAGTGGCGGATGCCATCGCCCGTTACCACCGCCTGCGGGGCAAGGATGTGCTGATGGTGACGGGAACCGACGAGCACG
GCCAGAAGATCCAGCGTACCGCCGAAGAGCGCGGCATTCCCCCTCAGCAGCACTGTGACGAGATTGCGGCTCAGTTTGCC
CAGCTTTGGCAGTTGCTAGACATCCGCTACGACCGCTTTATCCGCACCACCAACCCTCGCCACAAAGCCATTGTGCGGGA
GTTTTTTCAGCGGGTCTGGGATCAGGGAGATATTTTCATTGGCCGGCAGCAGGGTTGGTACTGTGTGGCCTGCGAAGAGT
TCAAAGACGAGGCCGAGCTGCTGGAGGGAGGATTTTGCCCCCTGCACCCCACCAAGAAGGTGGAGTGGAAAGACGAGGAA
AACTACTTCTTTCGCCTCTCCCGCTACCAAGAAAAGCTGGAGGCCCTGTATGCGGAGCACCCGGAGTTTATCCAACCAGA
AAGCCGCCGCAATGAGGTGATTAACTTTGTCAAGCAGGGCCTGCGGGACTTTTCCATCTCTCGCCCTAGTGTCTCCTGGG
GGATCCCTTTTCCGGCGGATCCCGGCCAAACCCTCTACGTCTGGTTTGACGCCCTGATCAACTACATCAGTGCCGCTCTG
GATCCTGACGACGAGCCCACCTTGGCAAATGCCCTACGCCGCTACTGGCCTGCGGATGTCCACCTGGTGGGCAAGGATAT
TGTCCGCTTCCACGCCATTTACTGGCCGGCCATGCTCATGTCCGCAGGTCTGCCCCTGCCCAAGCAGGTGTTTGGCCACG
GCTTTCTCACCAAAGATGGCCTCAAGATGAGCAAAAGCTTGGGGAACACGGTGGATCCCTTTGAGCTGGTGCAGCAATAC
GGCGCCGATGCCGTGCGCTACTACTTTCTGACGGAAATCGAGCTGGGCAAGGATGGCGATTTCAGCGAGGGCCGCTTCGT
TGACGTGCTCAACGCCGATCTGGCCAACGACCTGGGCAATCTGCTCAACCGCACCCTCAACATGCTCAGGAAATATCAAT
CCGACTACCGCGTTCCCGCCGTCGGGATCCCTGCAGATCACCCCCTCAAAACCCTGGCCGAGCGCCTTGCCCCGGCAGTG
GCCGACGCCTACGAGCGGCTGGATCCCAGCTCTGCTGCCCGCAAAGCCCTCAGCTTAGCCCAGGCCAGCAACAAATACCT
TGATGAGCAGGCTCCTTGGACGCTGCACAAGCAAGGGCAAAACGAACGGGTGGCCCAGATTCTCTACGCCGTTTTGGAAA
GTGTGCGCTGGGTGGCAGTCCTGCTCTCCCCCCTCATTCCCGCTCTCAGCCTCAAGATCCTGCAGCAGCTGGGGTTCTCC
CTCGCCGACACCCTGCAACTGCGCTGGGATCCCGATGCGACTTGGGGCCAGCTTCCCCCCGGGCAAGAGCCCCAGCCTCC
TACGCCTGTCTTTCAGAAGATCCTGAGGAACGCCTAG

Upstream 100 bases:

>100_bases
CTGAAGTTTCATCGAATGAGCCTCTCCCCACCCACGCTCTGGCTTGGCCTTTTGGACTTTCCGGCAACCTCTATCATGGG
AGAAAGCCTTGCGGATGCTC

Downstream 100 bases:

>100_bases
GGATTGGCGATGTCCGGTCGGGAAGATTGGGCAGCGGCTTGGGATCCCTCTGGTGTGGCTCCGCCGGCAAAAGGGCGGTC
ACCTCCCAGGGGAAGTGTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPDSTFVITTPIYYVNAAPHIGSAYTTLVADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGIPPQQHCDEIAAQFA
QLWQLLDIRYDRFIRTTNPRHKAIVREFFQRVWDQGDIFIGRQQGWYCVACEEFKDEAELLEGGFCPLHPTKKVEWKDEE
NYFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPGQTLYVWFDALINYISAAL
DPDDEPTLANALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPKQVFGHGFLTKDGLKMSKSLGNTVDPFELVQQY
GADAVRYYFLTEIELGKDGDFSEGRFVDVLNADLANDLGNLLNRTLNMLRKYQSDYRVPAVGIPADHPLKTLAERLAPAV
ADAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQNERVAQILYAVLESVRWVAVLLSPLIPALSLKILQQLGFS
LADTLQLRWDPDATWGQLPPGQEPQPPTPVFQKILRNA

Sequences:

>Translated_518_residues
MPDSTFVITTPIYYVNAAPHIGSAYTTLVADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGIPPQQHCDEIAAQFA
QLWQLLDIRYDRFIRTTNPRHKAIVREFFQRVWDQGDIFIGRQQGWYCVACEEFKDEAELLEGGFCPLHPTKKVEWKDEE
NYFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPGQTLYVWFDALINYISAAL
DPDDEPTLANALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPKQVFGHGFLTKDGLKMSKSLGNTVDPFELVQQY
GADAVRYYFLTEIELGKDGDFSEGRFVDVLNADLANDLGNLLNRTLNMLRKYQSDYRVPAVGIPADHPLKTLAERLAPAV
ADAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQNERVAQILYAVLESVRWVAVLLSPLIPALSLKILQQLGFS
LADTLQLRWDPDATWGQLPPGQEPQPPTPVFQKILRNA
>Mature_517_residues
PDSTFVITTPIYYVNAAPHIGSAYTTLVADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGIPPQQHCDEIAAQFAQ
LWQLLDIRYDRFIRTTNPRHKAIVREFFQRVWDQGDIFIGRQQGWYCVACEEFKDEAELLEGGFCPLHPTKKVEWKDEEN
YFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPGQTLYVWFDALINYISAALD
PDDEPTLANALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPKQVFGHGFLTKDGLKMSKSLGNTVDPFELVQQYG
ADAVRYYFLTEIELGKDGDFSEGRFVDVLNADLANDLGNLLNRTLNMLRKYQSDYRVPAVGIPADHPLKTLAERLAPAVA
DAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQNERVAQILYAVLESVRWVAVLLSPLIPALSLKILQQLGFSL
ADTLQLRWDPDATWGQLPPGQEPQPPTPVFQKILRNA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=37.8217821782178, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI14043022, Length=559, Percent_Identity=26.4758497316637, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1788432, Length=498, Percent_Identity=27.3092369477912, Blast_Score=208, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=34.4195519348269, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=31.7708333333333, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=562, Percent_Identity=27.4021352313167, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321610, Length=537, Percent_Identity=34.0782122905028, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6321704, Length=534, Percent_Identity=25.4681647940075, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21358483, Length=500, Percent_Identity=36.8, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=27.807486631016, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58970; Mature: 58838

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSTFVITTPIYYVNAAPHIGSAYTTLVADAIARYHRLRGKDVLMVTGTDEHGQKIQRT
CCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AEERGIPPQQHCDEIAAQFAQLWQLLDIRYDRFIRTTNPRHKAIVREFFQRVWDQGDIFI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEE
GRQQGWYCVACEEFKDEAELLEGGFCPLHPTKKVEWKDEENYFFRLSRYQEKLEALYAEH
ECCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPGQTLYVWFDALINYISAAL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
DPDDEPTLANALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPKQVFGHGFLTKDG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCH
LKMSKSLGNTVDPFELVQQYGADAVRYYFLTEIELGKDGDFSEGRFVDVLNADLANDLGN
HHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHH
LLNRTLNMLRKYQSDYRVPAVGIPADHPLKTLAERLAPAVADAYERLDPSSAARKALSLA
HHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QASNKYLDEQAPWTLHKQGQNERVAQILYAVLESVRWVAVLLSPLIPALSLKILQQLGFS
HHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LADTLQLRWDPDATWGQLPPGQEPQPPTPVFQKILRNA
HHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PDSTFVITTPIYYVNAAPHIGSAYTTLVADAIARYHRLRGKDVLMVTGTDEHGQKIQRT
CCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AEERGIPPQQHCDEIAAQFAQLWQLLDIRYDRFIRTTNPRHKAIVREFFQRVWDQGDIFI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEE
GRQQGWYCVACEEFKDEAELLEGGFCPLHPTKKVEWKDEENYFFRLSRYQEKLEALYAEH
ECCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPGQTLYVWFDALINYISAAL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
DPDDEPTLANALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPKQVFGHGFLTKDG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCH
LKMSKSLGNTVDPFELVQQYGADAVRYYFLTEIELGKDGDFSEGRFVDVLNADLANDLGN
HHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHH
LLNRTLNMLRKYQSDYRVPAVGIPADHPLKTLAERLAPAVADAYERLDPSSAARKALSLA
HHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QASNKYLDEQAPWTLHKQGQNERVAQILYAVLESVRWVAVLLSPLIPALSLKILQQLGFS
HHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LADTLQLRWDPDATWGQLPPGQEPQPPTPVFQKILRNA
HHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]