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The map label for this gene is pcm

Identifier: 86609629

GI number: 86609629

Start: 2289333

End: 2290067

Strand: Direct

Name: pcm

Synonym: CYB_2184

Alternate gene names: 86609629

Gene position: 2289333-2290067 (Clockwise)

Preceding gene: 86609626

Following gene: 86609631

Centisome position: 75.14

GC content: 58.1

Gene sequence:

>735_bases
GTGATCAACCCATTCTCATCCTTCCGGTGGAGACATTCTTCTCGCTCTCCAGCTGGGATCCCAGAAGTTGAACCTCAGCC
CCCAGACGCTTCCGATCCTTTTGCTTCCCAACGGGAGCAGATGGTCAAGAAACAGCTCATCAAACGAGGGATCCGAGATC
CACGGGTACTGGAAGCCATGGGAAAAGTGCCGCGTCACCTTTTTGTCCCCTCTGATCTGCGGGATCGGGCCTACAGGGAT
TGCCCTCTGCCCATCGGCTATGGCCAGACCATTTCTCAGCCCTATATTGTTGCTTTTATGACAGAAGCTGCTCGCCTCAC
GCCTGAATCGGTGGTGTTGGAAATCGGCACCGGCTCCGGCTATCAGGCGGCTGTTTTGGCAGAGCTGGCCCGGCAAGTGT
ATAGCCTGGAGCGTCTGTCCCCATTGGCTGCTCAAGCTCAACAAACCCTCGCTGCCCTGGGCTATCGCAACGTGGAAGTG
CGTCAGGGGGATGGCTACCAAGGTTGGCCAGAGCATGCTCCCTACGATGCCATTCTGGTGACGGCAGCGCCCCCAACTGT
GCCCATGGTGCTGCTGGATCAACTGGCGGTGGGGGGCACCTTGGTGGTGCCGGTGGGAGAAAGCCCTTCGGACTCCGGTG
CCCAGGGATCCCAGTCCTTGCTGATCCTCTGCAAAACAGCCCAAGGATGGGTTCAAAAAGGGGCTTTTCCAGTGCAGTTT
GTGCCCATGGTCTAG

Upstream 100 bases:

>100_bases
GGGGATCCATAAGCCCATAAAGCAAACAGCTGCGGTGTTATCCACAGCTTGAGCAGAGTCCAGATCTGAGCAGCTCATCG
TTTCTGGAGGTTGTGTATCG

Downstream 100 bases:

>100_bases
GGTTTTGCCCCCTCTATTTCTGCCAAAAGGTGGGCGCGAATTTGCAAAGCCAATTGGGGACGGTCGGTAATCAACCCTTG
AACAACGGGATTTTGTCCTA

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MINPFSSFRWRHSSRSPAGIPEVEPQPPDASDPFASQREQMVKKQLIKRGIRDPRVLEAMGKVPRHLFVPSDLRDRAYRD
CPLPIGYGQTISQPYIVAFMTEAARLTPESVVLEIGTGSGYQAAVLAELARQVYSLERLSPLAAQAQQTLAALGYRNVEV
RQGDGYQGWPEHAPYDAILVTAAPPTVPMVLLDQLAVGGTLVVPVGESPSDSGAQGSQSLLILCKTAQGWVQKGAFPVQF
VPMV

Sequences:

>Translated_244_residues
MINPFSSFRWRHSSRSPAGIPEVEPQPPDASDPFASQREQMVKKQLIKRGIRDPRVLEAMGKVPRHLFVPSDLRDRAYRD
CPLPIGYGQTISQPYIVAFMTEAARLTPESVVLEIGTGSGYQAAVLAELARQVYSLERLSPLAAQAQQTLAALGYRNVEV
RQGDGYQGWPEHAPYDAILVTAAPPTVPMVLLDQLAVGGTLVVPVGESPSDSGAQGSQSLLILCKTAQGWVQKGAFPVQF
VPMV
>Mature_244_residues
MINPFSSFRWRHSSRSPAGIPEVEPQPPDASDPFASQREQMVKKQLIKRGIRDPRVLEAMGKVPRHLFVPSDLRDRAYRD
CPLPIGYGQTISQPYIVAFMTEAARLTPESVVLEIGTGSGYQAAVLAELARQVYSLERLSPLAAQAQQTLAALGYRNVEV
RQGDGYQGWPEHAPYDAILVTAAPPTVPMVLLDQLAVGGTLVVPVGESPSDSGAQGSQSLLILCKTAQGWVQKGAFPVQF
VPMV

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=173, Percent_Identity=33.5260115606936, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=47.8672985781991, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71983477, Length=221, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=219, Percent_Identity=30.1369863013699, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17981723, Length=227, Percent_Identity=33.920704845815, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SYNJB (Q2JJN4)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478391.1
- ProteinModelPortal:   Q2JJN4
- SMR:   Q2JJN4
- STRING:   Q2JJN4
- GeneID:   3902885
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2184
- TIGR:   CYB_2184
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- PhylomeDB:   Q2JJN4
- ProtClustDB:   CLSK844542
- BioCyc:   SSP321332:CYB_2184-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 26519; Mature: 26519

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPFSSFRWRHSSRSPAGIPEVEPQPPDASDPFASQREQMVKKQLIKRGIRDPRVLEAM
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
GKVPRHLFVPSDLRDRAYRDCPLPIGYGQTISQPYIVAFMTEAARLTPESVVLEIGTGSG
HHCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCC
YQAAVLAELARQVYSLERLSPLAAQAQQTLAALGYRNVEVRQGDGYQGWPEHAPYDAILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEE
TAAPPTVPMVLLDQLAVGGTLVVPVGESPSDSGAQGSQSLLILCKTAQGWVQKGAFPVQF
ECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEE
VPMV
ECCC
>Mature Secondary Structure
MINPFSSFRWRHSSRSPAGIPEVEPQPPDASDPFASQREQMVKKQLIKRGIRDPRVLEAM
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
GKVPRHLFVPSDLRDRAYRDCPLPIGYGQTISQPYIVAFMTEAARLTPESVVLEIGTGSG
HHCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCC
YQAAVLAELARQVYSLERLSPLAAQAQQTLAALGYRNVEVRQGDGYQGWPEHAPYDAILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEE
TAAPPTVPMVLLDQLAVGGTLVVPVGESPSDSGAQGSQSLLILCKTAQGWVQKGAFPVQF
ECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEE
VPMV
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA