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The map label for this gene is glcF [H]

Identifier: 86609621

GI number: 86609621

Start: 2278764

End: 2280074

Strand: Reverse

Name: glcF [H]

Synonym: CYB_2175

Alternate gene names: 86609621

Gene position: 2280074-2278764 (Counterclockwise)

Preceding gene: 86609622

Following gene: 86609620

Centisome position: 74.84

GC content: 60.03

Gene sequence:

>1311_bases
ATGAGTTTATCGCAAGGGCCGGGTTTTGATGGGGTGGATCCCCCGGATCCGAAGCTGATCGATGCCTGTGTGCATTGTGG
GTTTTGCCTGACCACCTGCCCCAGCTACCGTGTAATCGGCAAAGAGACGGACTCGCCGCGGGGCCGCATCTACCAGATGG
ACGCCCTCAATCAGGGCCAAATCTCTCTCTCAGCGGCAGTGGTTTCCCACTTCGACAGTTGCTTGGGCTGTTTGGCCTGT
GTTACGGCCTGTCCATCGGGGGTGCAATATGACCGCTTAATTGCCGCCACCCGCCCGCAGATTGAGCGCAACTACTCCCG
CAGTTTTTGGGATCGGCTTTACCGCCAGTTCATCTTCTCGGTGTTTCCTTACCCGGAGCGGCTGCAACCGCTGCTGTGGC
CTCTCTGGCTGTACCAAAAAATGGGGATCGGCGCTTGGATCCGAAAGGGTCTGGAAAAGGTCTCCCCGCGTTTGGCGGCC
ATGGAATCCCTGATGCCCCCCCTGGAGGTGGAGCAACTGCTGCCCAAGCGTTACCCTGAGCGGATCCCGGCGGTGGGAGA
ACGCCGTTTTCGGGTGGGACTCATTTTGGGCTGCGTGCAGCGCCTGTTTGCCCCACAGGTAAATGAGGCCAGCATTCGCG
TCTTGACCCACAACGGCTGTGAGGTGGTGATCCCGCCTGTCCAGGGCTGTTGTGCGGCCTTGCCCCACCATCAAGGGCAA
CTGCAGCAGGCGGAAACCCTGGCCCGCCAGATGATCGATGCCTTTGCCGATTACGAGTTGGATGCCATTCTGATCACCGC
TTCCGGCTGCGGACATACCCTGAAAGACTATGGCCACCTATTGGCCAGGGATCCCGACTACAAAGAGAAGGCAGCCCGAT
TTGCCGCCCAGGTCAAGGACATTCAGGAGTTTCTCGCTCAGGTGGGGTTAACGGCTCCTCTACATCCGCTACAGCCGGAA
CCCCTGGTGCTGGTCTACCAGGATGCCTGTCACATGCTGCATGGGCAGCGGCTCAGCCAGGAACCCCGACACCTGCTGCG
GCAAATCCCAGGGCTGGAGCTGCGGGATCCCGTCGATGCCGCCCTCTGCTGTGGTAGCTCCGGCGTCTATAACCTGTTGC
AACCCCAGGTGGCGGATGAGCTGGGATCCCAGAAGGTGACGAACTTGCTCAACACTGGCGCCCAGGGGATCGTCTCCGCC
AATATCGGCTGCCGCCTACAAATCCAGAAACATCTGCGCCTACAGGGCAAAGCCGGGATCCCCATCTATCACCCGATGGA
ATTGTTGGATCGCGCCCTTACCGCTGCGTGA

Upstream 100 bases:

>100_bases
TCTGGGATTAAGATGCAGAAAAACTGCAGGCAGGATGGCAAGCTGCCAATTGTCTCCCTGCATGGCCATGTAGAGCTGAG
AGCGCGATAGGTCGAAGCCA

Downstream 100 bases:

>100_bases
CGCTTTCGCGAACAAGCTACAGGGGCTGCCTCTAGTCCCCGATCTGCTGAACAATTGGTTACTTTGCGGAACCGAAATAG
CCATTAAACTAAGCATGTTG

Product: glycolate oxidase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSLSQGPGFDGVDPPDPKLIDACVHCGFCLTTCPSYRVIGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDSCLGCLAC
VTACPSGVQYDRLIAATRPQIERNYSRSFWDRLYRQFIFSVFPYPERLQPLLWPLWLYQKMGIGAWIRKGLEKVSPRLAA
MESLMPPLEVEQLLPKRYPERIPAVGERRFRVGLILGCVQRLFAPQVNEASIRVLTHNGCEVVIPPVQGCCAALPHHQGQ
LQQAETLARQMIDAFADYELDAILITASGCGHTLKDYGHLLARDPDYKEKAARFAAQVKDIQEFLAQVGLTAPLHPLQPE
PLVLVYQDACHMLHGQRLSQEPRHLLRQIPGLELRDPVDAALCCGSSGVYNLLQPQVADELGSQKVTNLLNTGAQGIVSA
NIGCRLQIQKHLRLQGKAGIPIYHPMELLDRALTAA

Sequences:

>Translated_436_residues
MSLSQGPGFDGVDPPDPKLIDACVHCGFCLTTCPSYRVIGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDSCLGCLAC
VTACPSGVQYDRLIAATRPQIERNYSRSFWDRLYRQFIFSVFPYPERLQPLLWPLWLYQKMGIGAWIRKGLEKVSPRLAA
MESLMPPLEVEQLLPKRYPERIPAVGERRFRVGLILGCVQRLFAPQVNEASIRVLTHNGCEVVIPPVQGCCAALPHHQGQ
LQQAETLARQMIDAFADYELDAILITASGCGHTLKDYGHLLARDPDYKEKAARFAAQVKDIQEFLAQVGLTAPLHPLQPE
PLVLVYQDACHMLHGQRLSQEPRHLLRQIPGLELRDPVDAALCCGSSGVYNLLQPQVADELGSQKVTNLLNTGAQGIVSA
NIGCRLQIQKHLRLQGKAGIPIYHPMELLDRALTAA
>Mature_435_residues
SLSQGPGFDGVDPPDPKLIDACVHCGFCLTTCPSYRVIGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDSCLGCLACV
TACPSGVQYDRLIAATRPQIERNYSRSFWDRLYRQFIFSVFPYPERLQPLLWPLWLYQKMGIGAWIRKGLEKVSPRLAAM
ESLMPPLEVEQLLPKRYPERIPAVGERRFRVGLILGCVQRLFAPQVNEASIRVLTHNGCEVVIPPVQGCCAALPHHQGQL
QQAETLARQMIDAFADYELDAILITASGCGHTLKDYGHLLARDPDYKEKAARFAAQVKDIQEFLAQVGLTAPLHPLQPEP
LVLVYQDACHMLHGQRLSQEPRHLLRQIPGLELRDPVDAALCCGSSGVYNLLQPQVADELGSQKVTNLLNTGAQGIVSAN
IGCRLQIQKHLRLQGKAGIPIYHPMELLDRALTAA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=420, Percent_Identity=33.5714285714286, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786497, Length=241, Percent_Identity=26.1410788381743, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 48298; Mature: 48166

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSQGPGFDGVDPPDPKLIDACVHCGFCLTTCPSYRVIGKETDSPRGRIYQMDALNQGQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCC
ISLSAAVVSHFDSCLGCLACVTACPSGVQYDRLIAATRPQIERNYSRSFWDRLYRQFIFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFPYPERLQPLLWPLWLYQKMGIGAWIRKGLEKVSPRLAAMESLMPPLEVEQLLPKRYPE
HCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
RIPAVGERRFRVGLILGCVQRLFAPQVNEASIRVLTHNGCEVVIPPVQGCCAALPHHQGQ
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCH
LQQAETLARQMIDAFADYELDAILITASGCGHTLKDYGHLLARDPDYKEKAARFAAQVKD
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IQEFLAQVGLTAPLHPLQPEPLVLVYQDACHMLHGQRLSQEPRHLLRQIPGLELRDPVDA
HHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
ALCCGSSGVYNLLQPQVADELGSQKVTNLLNTGAQGIVSANIGCRLQIQKHLRLQGKAGI
HHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHCCCCCCC
PIYHPMELLDRALTAA
CCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLSQGPGFDGVDPPDPKLIDACVHCGFCLTTCPSYRVIGKETDSPRGRIYQMDALNQGQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCC
ISLSAAVVSHFDSCLGCLACVTACPSGVQYDRLIAATRPQIERNYSRSFWDRLYRQFIFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFPYPERLQPLLWPLWLYQKMGIGAWIRKGLEKVSPRLAAMESLMPPLEVEQLLPKRYPE
HCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
RIPAVGERRFRVGLILGCVQRLFAPQVNEASIRVLTHNGCEVVIPPVQGCCAALPHHQGQ
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCH
LQQAETLARQMIDAFADYELDAILITASGCGHTLKDYGHLLARDPDYKEKAARFAAQVKD
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IQEFLAQVGLTAPLHPLQPEPLVLVYQDACHMLHGQRLSQEPRHLLRQIPGLELRDPVDA
HHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
ALCCGSSGVYNLLQPQVADELGSQKVTNLLNTGAQGIVSANIGCRLQIQKHLRLQGKAGI
HHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHCCCCCCC
PIYHPMELLDRALTAA
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]