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The map label for this gene is tpiA

Identifier: 86609604

GI number: 86609604

Start: 2256715

End: 2257467

Strand: Direct

Name: tpiA

Synonym: CYB_2157

Alternate gene names: 86609604

Gene position: 2256715-2257467 (Clockwise)

Preceding gene: 86609602

Following gene: 86609606

Centisome position: 74.07

GC content: 60.42

Gene sequence:

>753_bases
TTGCGCCCCATCCTCATTGCCGGCAACTGGAAAATGCACAAGACTCAGGCTGAGGCTCGCGAGTTTCTGCGAGAGTTGGG
CCTGGCTTTACGGTCATCGCCGTCAGGGTCAGCATCCCAGCGGCAGATTATCTTGTGTGTGCCCTTTACAGACCTGGCGG
TGGTGGCAGAGCAAAGCCGCGGATCCGACATAGCCGTGGGAGCACAAAACCTACATTGGGAGGATCAGGGAGCTTTTACC
GGGGAAATTTCCGGCCCCATGCTGGCGGAATTGGGGGTGCGCTATGTCATCGTCGGCCACAGCGAACGGCGGCAGTACTT
CGGCGAGACGGACGAAACCGTCAATCGGCGTCTGGCAGCCGCCCAGCGGCACGGCCTTACCCCCATCCTCTGTGTGGGAG
AAAGCCAGCAGCAGCGGGAGCAAGGCTTGACCGAGTCTTGGATTGTGGGGCAGTTGGATCGCGCCTTGCAAGGGATCGAT
CTGGAGAACTTGGTGATTGCCTATGAGCCAATTTGGGCCATCGGCACCGGCCAGACTTGTGCAGCTCAAGAGGCCAACCG
GGTCATCGGCTTGATCCGGCAACACCTGGGCAATGCCCAACTGCCCATTTTGTATGGCGGCTCGGTGAAGGCCGGCAACA
TCGATGAGCTGATGGCCCAGCCTGAGATCGACGGCGTGTTGGTGGGAGGGGCCAGCCTAGACCCCCAAGAGTTTGCCCGC
ATCGTCAACTTTCAGGCCCTAGCTCCCGCCTAG

Upstream 100 bases:

>100_bases
AAAAACGAAAGCTGGGATCCCTGCTGTTCTTCCACATATTCCTTGGGCTTTCTGCTTGCGGGATCCCTTCCTTCTGCTGT
CTTTAAGGGAGGATCTCATC

Downstream 100 bases:

>100_bases
GCCGCTAGCGGGATTGTCCCCACAGCAGGGCCACCGGGAAGGATTTCAAGACAACGCCCACCGGCAAGGTTTCGCCGGCA
GGGATCCTTTCCTGGGTTAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRPILIAGNWKMHKTQAEAREFLRELGLALRSSPSGSASQRQIILCVPFTDLAVVAEQSRGSDIAVGAQNLHWEDQGAFT
GEISGPMLAELGVRYVIVGHSERRQYFGETDETVNRRLAAAQRHGLTPILCVGESQQQREQGLTESWIVGQLDRALQGID
LENLVIAYEPIWAIGTGQTCAAQEANRVIGLIRQHLGNAQLPILYGGSVKAGNIDELMAQPEIDGVLVGGASLDPQEFAR
IVNFQALAPA

Sequences:

>Translated_250_residues
MRPILIAGNWKMHKTQAEAREFLRELGLALRSSPSGSASQRQIILCVPFTDLAVVAEQSRGSDIAVGAQNLHWEDQGAFT
GEISGPMLAELGVRYVIVGHSERRQYFGETDETVNRRLAAAQRHGLTPILCVGESQQQREQGLTESWIVGQLDRALQGID
LENLVIAYEPIWAIGTGQTCAAQEANRVIGLIRQHLGNAQLPILYGGSVKAGNIDELMAQPEIDGVLVGGASLDPQEFAR
IVNFQALAPA
>Mature_250_residues
MRPILIAGNWKMHKTQAEAREFLRELGLALRSSPSGSASQRQIILCVPFTDLAVVAEQSRGSDIAVGAQNLHWEDQGAFT
GEISGPMLAELGVRYVIVGHSERRQYFGETDETVNRRLAAAQRHGLTPILCVGESQQQREQGLTESWIVGQLDRALQGID
LENLVIAYEPIWAIGTGQTCAAQEANRVIGLIRQHLGNAQLPILYGGSVKAGNIDELMAQPEIDGVLVGGASLDPQEFAR
IVNFQALAPA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=39.6, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=39.6, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1790353, Length=252, Percent_Identity=42.0634920634921, Blast_Score=191, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=39.6, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=250, Percent_Identity=35.6, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=40, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=40, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=40, Blast_Score=167, Evalue=5e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SYNJB (Q2JJQ9)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478366.1
- STRING:   Q2JJQ9
- GeneID:   3902590
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2157
- TIGR:   CYB_2157
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   CIGENLD
- PhylomeDB:   Q2JJQ9
- ProtClustDB:   PRK00042
- BioCyc:   SSP321332:CYB_2157-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27116; Mature: 27116

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00171 TIM_1; PS51440 TIM_2; PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: ACT_SITE 100-100 ACT_SITE 169-169 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPILIAGNWKMHKTQAEAREFLRELGLALRSSPSGSASQRQIILCVPFTDLAVVAEQSR
CCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEHHHHCCC
GSDIAVGAQNLHWEDQGAFTGEISGPMLAELGVRYVIVGHSERRQYFGETDETVNRRLAA
CCEEEECCCCCCCCCCCCEEECCCCCHHHHCCCEEEEEECHHHHHHCCCCHHHHHHHHHH
AQRHGLTPILCVGESQQQREQGLTESWIVGQLDRALQGIDLENLVIAYEPIWAIGTGQTC
HHHCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCH
AAQEANRVIGLIRQHLGNAQLPILYGGSVKAGNIDELMAQPEIDGVLVGGASLDPQEFAR
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEECCCCCHHHHHH
IVNFQALAPA
HHCCCCCCCC
>Mature Secondary Structure
MRPILIAGNWKMHKTQAEAREFLRELGLALRSSPSGSASQRQIILCVPFTDLAVVAEQSR
CCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEHHHHCCC
GSDIAVGAQNLHWEDQGAFTGEISGPMLAELGVRYVIVGHSERRQYFGETDETVNRRLAA
CCEEEECCCCCCCCCCCCEEECCCCCHHHHCCCEEEEEECHHHHHHCCCCHHHHHHHHHH
AQRHGLTPILCVGESQQQREQGLTESWIVGQLDRALQGIDLENLVIAYEPIWAIGTGQTC
HHHCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCH
AAQEANRVIGLIRQHLGNAQLPILYGGSVKAGNIDELMAQPEIDGVLVGGASLDPQEFAR
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEECCCCCHHHHHH
IVNFQALAPA
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA