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The map label for this gene is pilT-2 [H]

Identifier: 86609589

GI number: 86609589

Start: 2239851

End: 2240981

Strand: Reverse

Name: pilT-2 [H]

Synonym: CYB_2142

Alternate gene names: 86609589

Gene position: 2240981-2239851 (Counterclockwise)

Preceding gene: 86609590

Following gene: 86609588

Centisome position: 73.55

GC content: 60.21

Gene sequence:

>1131_bases
ATGATCGAAGACCTCATGGAAGCAATCGTCCAAAAGGGCGGTTCCGACATTCATATCTCGGCGGGTTTGCCCCCCTACTT
CCGCATCAATGGTCACCTTGAGCCGACAGAGCATGAGCCTCTCTCGGCAGAGGAGGTGCAGCGGTTGATCTTCAGCATGC
TGAACAACAACCAGCGCAAGCAGCTGGAGCAAAACTGGGAATTGGACTGCTCCTATGGGGTGCGGGGGATCGGGCGCTTC
CGCGTGAACGTCTACCGCGATCGCGGCACCTATGCTTGCGCCATGCGGGCCCTGGCTTCTGAGATCCCCAACATCGACAA
GCTGGGCCTGCCGGATGTGTGTAAGGAAGCAGCTCGCCTGCCGCGGGGCTTGGTGCTGGTAACCGGCCCAACGGGATCTG
GCAAATCGACTACGCTGGCTTCCATGATCGACATGATCAACCGCGAACGGGCCGAGCACATCCTCACCATCGAGGATCCC
ATTGAGTACATCTACACCCCCATTCGCAGCATCATCCACCAGCGGCAACTGGGGGAAGACACTAAGAGTTTTGCCAATGC
CCTGCGGGCAGCGATGCGGGAGGATCCGGATGTGATCCTGATCGGGGAGATGCGGGATCTGGAGACCATCCAGTTGGCCA
TTACCGCCGCCGAGACGGGCCACCTCTGCTTTGCCACGGTACACACCAGTTCTGCCGCCCAGACGGTGGATCGCTTGGTG
GATGTGTTTCCGCCCGAGCAGCAGCAGCAGATTCGCGTGCAGCTCTCCAACTCCCTCAAGGCCGTCTTCTCCCAAACTCT
GGCCCGCCGCCTCAATCCCAAGCCAGGAGAGCCTGGACGGGTGCTGGTGCAGGAAATCATGGTGGTGACGCCGGCCATTG
CCAACCTGATTCGGGAGGGCAAAACTGCCCAGATCTACTCGGCTATCCAGACGGGGGGCAAGCAGGGCATGAAAACGCTG
GAGCAAGATCTGGCTAACCTTTACCTACAGGGCAAGATCGACTTCGATACGGCCATGTCTAAGACCTCTCGCCCTGAAGA
GCTGCAGCGGCTTTTGGGCAACGTTACCGGGCCCTCGCCAGCCGCGGCTGCCCAACAACAGCGGCCAGCAGCAGTTGCCG
CCCGTCGCTAA

Upstream 100 bases:

>100_bases
CAGTCCTATACAGTTGCCTTTCTCACATGGCTTTGGCCGGCGGCTCTTTTGATAGACAGTTCTACTCGCCTTTGGAGAAC
CCAACCCTATGCCCCAACTG

Downstream 100 bases:

>100_bases
ACTTCGTCTCCACCAGGCAGCGCTGGGATCCCGGGGTGAGGGTTCATTGCCGATCTCTAGCCGCTGACTTGATTTGTGGC
TAAGCTCTAGCTGAGAGATT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIEDLMEAIVQKGGSDIHISAGLPPYFRINGHLEPTEHEPLSAEEVQRLIFSMLNNNQRKQLEQNWELDCSYGVRGIGRF
RVNVYRDRGTYACAMRALASEIPNIDKLGLPDVCKEAARLPRGLVLVTGPTGSGKSTTLASMIDMINRERAEHILTIEDP
IEYIYTPIRSIIHQRQLGEDTKSFANALRAAMREDPDVILIGEMRDLETIQLAITAAETGHLCFATVHTSSAAQTVDRLV
DVFPPEQQQQIRVQLSNSLKAVFSQTLARRLNPKPGEPGRVLVQEIMVVTPAIANLIREGKTAQIYSAIQTGGKQGMKTL
EQDLANLYLQGKIDFDTAMSKTSRPEELQRLLGNVTGPSPAAAAQQQRPAAVAARR

Sequences:

>Translated_376_residues
MIEDLMEAIVQKGGSDIHISAGLPPYFRINGHLEPTEHEPLSAEEVQRLIFSMLNNNQRKQLEQNWELDCSYGVRGIGRF
RVNVYRDRGTYACAMRALASEIPNIDKLGLPDVCKEAARLPRGLVLVTGPTGSGKSTTLASMIDMINRERAEHILTIEDP
IEYIYTPIRSIIHQRQLGEDTKSFANALRAAMREDPDVILIGEMRDLETIQLAITAAETGHLCFATVHTSSAAQTVDRLV
DVFPPEQQQQIRVQLSNSLKAVFSQTLARRLNPKPGEPGRVLVQEIMVVTPAIANLIREGKTAQIYSAIQTGGKQGMKTL
EQDLANLYLQGKIDFDTAMSKTSRPEELQRLLGNVTGPSPAAAAQQQRPAAVAARR
>Mature_376_residues
MIEDLMEAIVQKGGSDIHISAGLPPYFRINGHLEPTEHEPLSAEEVQRLIFSMLNNNQRKQLEQNWELDCSYGVRGIGRF
RVNVYRDRGTYACAMRALASEIPNIDKLGLPDVCKEAARLPRGLVLVTGPTGSGKSTTLASMIDMINRERAEHILTIEDP
IEYIYTPIRSIIHQRQLGEDTKSFANALRAAMREDPDVILIGEMRDLETIQLAITAAETGHLCFATVHTSSAAQTVDRLV
DVFPPEQQQQIRVQLSNSLKAVFSQTLARRLNPKPGEPGRVLVQEIMVVTPAIANLIREGKTAQIYSAIQTGGKQGMKTL
EQDLANLYLQGKIDFDTAMSKTSRPEELQRLLGNVTGPSPAAAAQQQRPAAVAARR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=332, Percent_Identity=43.9759036144578, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1789723, Length=395, Percent_Identity=30.6329113924051, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786296, Length=392, Percent_Identity=30.3571428571429, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41542; Mature: 41542

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEDLMEAIVQKGGSDIHISAGLPPYFRINGHLEPTEHEPLSAEEVQRLIFSMLNNNQRK
CHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
QLEQNWELDCSYGVRGIGRFRVNVYRDRGTYACAMRALASEIPNIDKLGLPDVCKEAARL
HHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
PRGLVLVTGPTGSGKSTTLASMIDMINRERAEHILTIEDPIEYIYTPIRSIIHQRQLGED
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCHH
TKSFANALRAAMREDPDVILIGEMRDLETIQLAITAAETGHLCFATVHTSSAAQTVDRLV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHH
DVFPPEQQQQIRVQLSNSLKAVFSQTLARRLNPKPGEPGRVLVQEIMVVTPAIANLIREG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KTAQIYSAIQTGGKQGMKTLEQDLANLYLQGKIDFDTAMSKTSRPEELQRLLGNVTGPSP
CHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCC
AAAAQQQRPAAVAARR
HHHHHHCCCCHHCCCC
>Mature Secondary Structure
MIEDLMEAIVQKGGSDIHISAGLPPYFRINGHLEPTEHEPLSAEEVQRLIFSMLNNNQRK
CHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
QLEQNWELDCSYGVRGIGRFRVNVYRDRGTYACAMRALASEIPNIDKLGLPDVCKEAARL
HHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
PRGLVLVTGPTGSGKSTTLASMIDMINRERAEHILTIEDPIEYIYTPIRSIIHQRQLGED
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCHH
TKSFANALRAAMREDPDVILIGEMRDLETIQLAITAAETGHLCFATVHTSSAAQTVDRLV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHH
DVFPPEQQQQIRVQLSNSLKAVFSQTLARRLNPKPGEPGRVLVQEIMVVTPAIANLIREG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KTAQIYSAIQTGGKQGMKTLEQDLANLYLQGKIDFDTAMSKTSRPEELQRLLGNVTGPSP
CHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCC
AAAAQQQRPAAVAARR
HHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]