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The map label for this gene is def-1 [H]

Identifier: 86609579

GI number: 86609579

Start: 2226600

End: 2227124

Strand: Direct

Name: def-1 [H]

Synonym: CYB_2132

Alternate gene names: 86609579

Gene position: 2226600-2227124 (Clockwise)

Preceding gene: 86609578

Following gene: 86609580

Centisome position: 73.08

GC content: 60.0

Gene sequence:

>525_bases
ATGACTTTTCGCCTACGCCAATTGGGGGATCCCATCCTGACGCAAGTGGCCGAGCCGGTGACGGAGTTCAAAACGCCGGC
CTTGCAGGATCTTATAGAAGGCATGCTGGCTACCCTGAAAGAGGCGCAGGGGGTGGGCTTGGCTGCTCCTCAGGTGGGAT
CCCTGCTCCAAGTGCTGATCGTGGCCTCTCGTCCCAATCCCCGCTACCCGGAAGCGCCGCAAATGCAGCCTCTGGTGATG
GTTAACCCCCGTCTGCTGGCTTGCAGTTCTGAACAGGTGCTGGGTTGGGAAGGCTGCTTGAGTGTGCCCAATTGCCGTGG
TCTGGTGGCCCGCGCCCGCGAAGTGGAGGTGGAGTATTACACTCCAGAAGGCGTTCAGCAAAGGGTGGTCTGGCAGGATT
TTCCGGCGCGGATCTTTCAGCACGAATACGATCACCTGATGGGTCGGGTGTTCCTGCAGCGGCAGCCTCGGCAACTGCTT
ACCGAAGCCCAGTACCAAGCACAGATTTTGGGTCAGCCCGCCTGA

Upstream 100 bases:

>100_bases
CGCTCCAAGCTGGCCCTGGAACTGGTGGCTACTGGCAAGAGCCGCTAGGCTGCTCAGGGATCCCCGCTAGACTGAGAAGA
AGAATTGCTTGGGGCAAGCC

Downstream 100 bases:

>100_bases
GGGATCCCACCATGCCCTACATGGTTATCTACGACGGCCTCTGCAACCTCTGTGCCACCGGCGTTCAGCTCTTGGAGCAA
CTGGATCGCGGACGGCAGTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTFRLRQLGDPILTQVAEPVTEFKTPALQDLIEGMLATLKEAQGVGLAAPQVGSLLQVLIVASRPNPRYPEAPQMQPLVM
VNPRLLACSSEQVLGWEGCLSVPNCRGLVARAREVEVEYYTPEGVQQRVVWQDFPARIFQHEYDHLMGRVFLQRQPRQLL
TEAQYQAQILGQPA

Sequences:

>Translated_174_residues
MTFRLRQLGDPILTQVAEPVTEFKTPALQDLIEGMLATLKEAQGVGLAAPQVGSLLQVLIVASRPNPRYPEAPQMQPLVM
VNPRLLACSSEQVLGWEGCLSVPNCRGLVARAREVEVEYYTPEGVQQRVVWQDFPARIFQHEYDHLMGRVFLQRQPRQLL
TEAQYQAQILGQPA
>Mature_173_residues
TFRLRQLGDPILTQVAEPVTEFKTPALQDLIEGMLATLKEAQGVGLAAPQVGSLLQVLIVASRPNPRYPEAPQMQPLVMV
NPRLLACSSEQVLGWEGCLSVPNCRGLVARAREVEVEYYTPEGVQQRVVWQDFPARIFQHEYDHLMGRVFLQRQPRQLLT
EAQYQAQILGQPA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=172, Percent_Identity=36.046511627907, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1789682, Length=144, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645726, Length=172, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19547; Mature: 19416

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRLRQLGDPILTQVAEPVTEFKTPALQDLIEGMLATLKEAQGVGLAAPQVGSLLQVLI
CCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VASRPNPRYPEAPQMQPLVMVNPRLLACSSEQVLGWEGCLSVPNCRGLVARAREVEVEYY
HHCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHEEEEEE
TPEGVQQRVVWQDFPARIFQHEYDHLMGRVFLQRQPRQLLTEAQYQAQILGQPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TFRLRQLGDPILTQVAEPVTEFKTPALQDLIEGMLATLKEAQGVGLAAPQVGSLLQVLI
CCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VASRPNPRYPEAPQMQPLVMVNPRLLACSSEQVLGWEGCLSVPNCRGLVARAREVEVEYY
HHCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHEEEEEE
TPEGVQQRVVWQDFPARIFQHEYDHLMGRVFLQRQPRQLLTEAQYQAQILGQPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]