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The map label for this gene is purF [H]

Identifier: 86609578

GI number: 86609578

Start: 2224919

End: 2226547

Strand: Direct

Name: purF [H]

Synonym: CYB_2131

Alternate gene names: 86609578

Gene position: 2224919-2226547 (Clockwise)

Preceding gene: 86609577

Following gene: 86609579

Centisome position: 73.03

GC content: 61.57

Gene sequence:

>1629_bases
ATGGCAGCAAGGGCGGGAGCAGAACCATCTTCTGGCTGTGGAGAAGCAGAGGGGCAGACTGCCGTTGGCGTTGATGCCCT
GTACCCCTCAGAAAACATCAGCGGGGCGGGGTATGCCCGGGAAGAGCTGCAAGAGGCTTGCGGGGTCTTTGGCATCCTGG
CTCCAGGAGAGGAGGTGGCCAAGCTGGCCTACTTTGGCCTATTTGCCTTGCAGCATCGCGGCCAAGAATCGGCAGGGATC
GCCACTTTTGAGGGATCCTTTTGCCGGGTTCACAAGGCGATGGGCTTGGTCTCCCAGGTCTTCGATGAAGTGAACTTGGC
CCAACTGACCGGGGATCTGGCGGTTGGTCACACCCGCTACTCCACCACTGGATCCAGCCGTGTGGCCAATGCTCAGCCGG
TGATCGTGGAGACTCGCCTGGGGCCGCTGGCTCTGGCCCACAACGGCAATTTGGTCAATGCGGAAGAGCTGCGGAAGGAA
CTGGAGGCCGCCGACCGGCACCTAACCAGCAGCACCGACTCCGAGTGTATTGCCCACGCCATTGCCCAGGCGGTGAACCA
AGGTCAAGATTGGATCGCGGCCACTTGCCAAGCTTTGCGCCGCTGTCAGGGGGCTTTTAGTCTGGTGATCGGCACGCCAG
AGGGCCTGATCGGGGCACGGGATCCCTATGGGGTGCGTCCGCTGGTGTTGGGGCTTTTGAGCAGCAACCCCGCTCTGGAT
GAGCTGCTGCAGTCAGAGATGCTGGAGATCATCTGCAGCGACGGCACCCTCAGCCACCCCGAGGCCGAGCCGCTGCATTA
CGTGTTGGCTTCAGAAACCTGTGCTTTGGATATCATTGGGGCCGACCCTCTGCGGCAGGTGGAGCCGGGAGAGTTGGTGT
GGATCAGCCGTCAGGGGCTGCAATCGGTGCGTTGGGCGGAGGCCACCCCCAAGTTGTGCATCTTCGAGATGATCTACTTC
GCCCGCCCCGACAGTTGGATGCACGGGGAAAGCCTCTACTCCTACCGGGTGCGGCTGGGAGAACAACTGGCCAAGGAGGC
TCCGGCAGAGGCCGACTGGGTGATCTCCGTTCCCGATTCCGGCACGCCGGCGGCCATTGGCTATGCTCGCCAGTCGGGGA
TCCCTTACACCGAAGGGCTGATCAAAAATCGCTACGTGGGCCGCACCTTTATTCAGCCCACCCAATCCATGCGGGAGCGG
GGCATTCGCATGAAGCTCAACCCCCTGGAGGATGTGCTGGGGGGGAAGCGGATTGTTATTGTGGACGACTCGATTGTGCG
GGGTACCACCAGCCAGAAAATCGTCAAGGCCCTGCGCCAGGCGGGGGCTACCCAGGTCCACATGCGCATTTCCAGCCCCC
CGGTCACCCATCCCTGCTTCTACGGTATCGATACCGATAGCCAGGATCAGTTGATTGCTGCCCAGCATTCCGTAGAGGAA
ATCGCCGAGAAGATTGGAGTGGATTCTTTGGCCTACCTGAGCTGGGAAGGCATGCTGGCTGCCACGGGCCGGGATCCCAA
CTCGTTCTGCTCCGCTTGCTTTACCGGCCACTACCCCATCCCCATCCCAGAAGGGCTGAAGCGCTCCAAGCTGGCCCTGG
AACTGGTGGCTACTGGCAAGAGCCGCTAG

Upstream 100 bases:

>100_bases
AAACCCCACCTGTGGACTCCCCCTTTGGGGGTGAAGCCAGATAGAGGGGAATGACCTGGGCCGAGTTAGACTGGTAAGCG
TGACAGCAAGACGAGGATCC

Downstream 100 bases:

>100_bases
GCTGCTCAGGGATCCCCGCTAGACTGAGAAGAAGAATTGCTTGGGGCAAGCCATGACTTTTCGCCTACGCCAATTGGGGG
ATCCCATCCTGACGCAAGTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAARAGAEPSSGCGEAEGQTAVGVDALYPSENISGAGYAREELQEACGVFGILAPGEEVAKLAYFGLFALQHRGQESAGI
ATFEGSFCRVHKAMGLVSQVFDEVNLAQLTGDLAVGHTRYSTTGSSRVANAQPVIVETRLGPLALAHNGNLVNAEELRKE
LEAADRHLTSSTDSECIAHAIAQAVNQGQDWIAATCQALRRCQGAFSLVIGTPEGLIGARDPYGVRPLVLGLLSSNPALD
ELLQSEMLEIICSDGTLSHPEAEPLHYVLASETCALDIIGADPLRQVEPGELVWISRQGLQSVRWAEATPKLCIFEMIYF
ARPDSWMHGESLYSYRVRLGEQLAKEAPAEADWVISVPDSGTPAAIGYARQSGIPYTEGLIKNRYVGRTFIQPTQSMRER
GIRMKLNPLEDVLGGKRIVIVDDSIVRGTTSQKIVKALRQAGATQVHMRISSPPVTHPCFYGIDTDSQDQLIAAQHSVEE
IAEKIGVDSLAYLSWEGMLAATGRDPNSFCSACFTGHYPIPIPEGLKRSKLALELVATGKSR

Sequences:

>Translated_542_residues
MAARAGAEPSSGCGEAEGQTAVGVDALYPSENISGAGYAREELQEACGVFGILAPGEEVAKLAYFGLFALQHRGQESAGI
ATFEGSFCRVHKAMGLVSQVFDEVNLAQLTGDLAVGHTRYSTTGSSRVANAQPVIVETRLGPLALAHNGNLVNAEELRKE
LEAADRHLTSSTDSECIAHAIAQAVNQGQDWIAATCQALRRCQGAFSLVIGTPEGLIGARDPYGVRPLVLGLLSSNPALD
ELLQSEMLEIICSDGTLSHPEAEPLHYVLASETCALDIIGADPLRQVEPGELVWISRQGLQSVRWAEATPKLCIFEMIYF
ARPDSWMHGESLYSYRVRLGEQLAKEAPAEADWVISVPDSGTPAAIGYARQSGIPYTEGLIKNRYVGRTFIQPTQSMRER
GIRMKLNPLEDVLGGKRIVIVDDSIVRGTTSQKIVKALRQAGATQVHMRISSPPVTHPCFYGIDTDSQDQLIAAQHSVEE
IAEKIGVDSLAYLSWEGMLAATGRDPNSFCSACFTGHYPIPIPEGLKRSKLALELVATGKSR
>Mature_541_residues
AARAGAEPSSGCGEAEGQTAVGVDALYPSENISGAGYAREELQEACGVFGILAPGEEVAKLAYFGLFALQHRGQESAGIA
TFEGSFCRVHKAMGLVSQVFDEVNLAQLTGDLAVGHTRYSTTGSSRVANAQPVIVETRLGPLALAHNGNLVNAEELRKEL
EAADRHLTSSTDSECIAHAIAQAVNQGQDWIAATCQALRRCQGAFSLVIGTPEGLIGARDPYGVRPLVLGLLSSNPALDE
LLQSEMLEIICSDGTLSHPEAEPLHYVLASETCALDIIGADPLRQVEPGELVWISRQGLQSVRWAEATPKLCIFEMIYFA
RPDSWMHGESLYSYRVRLGEQLAKEAPAEADWVISVPDSGTPAAIGYARQSGIPYTEGLIKNRYVGRTFIQPTQSMRERG
IRMKLNPLEDVLGGKRIVIVDDSIVRGTTSQKIVKALRQAGATQVHMRISSPPVTHPCFYGIDTDSQDQLIAAQHSVEEI
AEKIGVDSLAYLSWEGMLAATGRDPNSFCSACFTGHYPIPIPEGLKRSKLALELVATGKSR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=523, Percent_Identity=42.2562141491396, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1788651, Length=494, Percent_Identity=38.8663967611336, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1790167, Length=191, Percent_Identity=34.0314136125654, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=505, Percent_Identity=40.7920792079208, Blast_Score=337, Evalue=7e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=506, Percent_Identity=36.9565217391304, Blast_Score=282, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24659598, Length=504, Percent_Identity=42.8571428571429, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28573187, Length=536, Percent_Identity=43.0970149253731, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24659604, Length=481, Percent_Identity=43.4511434511435, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 58298; Mature: 58167

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAGAEPSSGCGEAEGQTAVGVDALYPSENISGAGYAREELQEACGVFGILAPGEEVA
CCCCCCCCCCCCCCCCCCCEEECEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHH
KLAYFGLFALQHRGQESAGIATFEGSFCRVHKAMGLVSQVFDEVNLAQLTGDLAVGHTRY
HHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCEEE
STTGSSRVANAQPVIVETRLGPLALAHNGNLVNAEELRKELEAADRHLTSSTDSECIAHA
CCCCCCCCCCCCCEEEEECCCCEEEECCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAQAVNQGQDWIAATCQALRRCQGAFSLVIGTPEGLIGARDPYGVRPLVLGLLSSNPALD
HHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHH
ELLQSEMLEIICSDGTLSHPEAEPLHYVLASETCALDIIGADPLRQVEPGELVWISRQGL
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEEHHCH
QSVRWAEATPKLCIFEMIYFARPDSWMHGESLYSYRVRLGEQLAKEAPAEADWVISVPDS
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
GTPAAIGYARQSGIPYTEGLIKNRYVGRTFIQPTQSMRERGIRMKLNPLEDVLGGKRIVI
CCCCCEEHHHHCCCCHHHHHHHCCCCCCHHHCCHHHHHHCCCEEEECHHHHHHCCCEEEE
VDDSIVRGTTSQKIVKALRQAGATQVHMRISSPPVTHPCFYGIDTDSQDQLIAAQHSVEE
ECCCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHH
IAEKIGVDSLAYLSWEGMLAATGRDPNSFCSACFTGHYPIPIPEGLKRSKLALELVATGK
HHHHHCCCCEEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEECCC
SR
CC
>Mature Secondary Structure 
AARAGAEPSSGCGEAEGQTAVGVDALYPSENISGAGYAREELQEACGVFGILAPGEEVA
CCCCCCCCCCCCCCCCCCEEECEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHH
KLAYFGLFALQHRGQESAGIATFEGSFCRVHKAMGLVSQVFDEVNLAQLTGDLAVGHTRY
HHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCEEE
STTGSSRVANAQPVIVETRLGPLALAHNGNLVNAEELRKELEAADRHLTSSTDSECIAHA
CCCCCCCCCCCCCEEEEECCCCEEEECCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAQAVNQGQDWIAATCQALRRCQGAFSLVIGTPEGLIGARDPYGVRPLVLGLLSSNPALD
HHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHH
ELLQSEMLEIICSDGTLSHPEAEPLHYVLASETCALDIIGADPLRQVEPGELVWISRQGL
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEEHHCH
QSVRWAEATPKLCIFEMIYFARPDSWMHGESLYSYRVRLGEQLAKEAPAEADWVISVPDS
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
GTPAAIGYARQSGIPYTEGLIKNRYVGRTFIQPTQSMRERGIRMKLNPLEDVLGGKRIVI
CCCCCEEHHHHCCCCHHHHHHHCCCCCCHHHCCHHHHHHCCCEEEECHHHHHHCCCEEEE
VDDSIVRGTTSQKIVKALRQAGATQVHMRISSPPVTHPCFYGIDTDSQDQLIAAQHSVEE
ECCCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHH
IAEKIGVDSLAYLSWEGMLAATGRDPNSFCSACFTGHYPIPIPEGLKRSKLALELVATGK
HHHHHCCCCEEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEECCC
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]