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The map label for this gene is rsbU [H]

Identifier: 86609514

GI number: 86609514

Start: 2163803

End: 2165248

Strand: Direct

Name: rsbU [H]

Synonym: CYB_2066

Alternate gene names: 86609514

Gene position: 2163803-2165248 (Clockwise)

Preceding gene: 86609513

Following gene: 86609515

Centisome position: 71.02

GC content: 59.13

Gene sequence:

>1446_bases
GTGCCTGCCCCTGCAATGCCGGATCGGGTGGCAGAACTGGAGAGAGAACACCATCGGATGCAGTATCTGCTGGCGTGGCT
GGCTTTTGCTCTGCGCTCTTTTAACAACTTGAACCAGATCCTGGAGCTGACGGCTTTTGTGGCCAGCCAACTGACCGATG
CCGAAGGCGGAGCCCTGGTTTTGTTCAATCCCAACGGCACCATCCAGTTGGATCAACTGCATTGTTCCGATCTCAATCAC
CGCGAGCAGATTCGGGCTGAGATGGAGCAAATTACCCAGACCCTCTCGGCTCAAGAGCCCATCCTCAAAACGGAGGGATC
CCTGTCTGTTAAGATCCCTGCCCTGGAACACCTCTTGGACGAACGGTTCCGCCAGAGCTTGGGGCCGGATGTACGCCTTT
TCGGCACGCCGCTGCTGATTCGCAACTCTGTCCAGGGTCGCTTGTACGTCTTCAGCCGTCAGCCTGGCTACCACTGGGAT
GAACGTCGCCAACAACTGTTGCGCTTGGTCGCCGATCAGGCAGGGGTGGCCATCGAAAATGACCGCTTGACGGCAGCCTT
GCGCCGCAAGATGGCCCTGGAAAAAGAAATGGAGATTGGCTCTTACATCCAGACCCAACTGCTACCTCGGGAGTGCCCTC
AAGTCAAGGGGATTCGCTTGGCAGCCCGTTGCCGGGTGGCCAGCAAAGTGGGGGGCGACTACTATGACTTCATCAAGATT
CCCGGCGAACGCCTGGAAACATCCGACTCATCTGAAGAAACGGCAGGCTTTCGCTTGGCCATCGTCATTGGGGATGTGAT
GGGCAAAGGGGTGCCCGCCGGCCTGTTGATGACCATGACCCGCGCCATGGTGCGGGCGGAGGCCCTCAACCGCCATTCGC
CGGCCCGTGTTCTGCAACATCTGAACCGGGCCATGTACAGTGACCTGGAAAACTCCAATCGCTTCATCAGCCTGTTTTAT
TCGGAGTACGATCCCCGCTCCCATCGCCTCTGCTTCAGCAACGCGGCCCACAACCCCACCCTGTGGTGGCAGGCCCGCAC
CCGAGAAATTCAACCCCTGGACACAGAGGGGGCGCTGATCGGCTTGGAAGAAAACAGCCACTACTCGGAAAGCTGCGTGC
AACTGCAACCCGGGGATGTGGTGGTGTACTACACCGATGGCTACACGGAGACAGCCAACACCCGCGGCGAGCGCTTGGAA
ACCTCTGGGCTGATGGACGCCATCCAGTACGCTGCCCGTCGCTACGACTCCCCCGAAGACATTTTGGAGAGCTTGTTTGA
GCAGATGGATGCCTTCCGAGGCCGTCGGGGATCCCATCCTCTTCCCCTATGGCTGCCCGAGGATCCAGAGCTCCTGTTGC
AGGATGAAATTTCCCTGCGGCCTGAGCCCGTGGACGACACAACGCTGGTGGTGTTGAAGGTGTTGCCCGACTCCGAGCCA
GCCTAG

Upstream 100 bases:

>100_bases
GGACTTGCGCTCAGCTCAGACCGGCCAGGATGAGGATCCCACCTCTTCAGGTGCCGTTGCGTCCGATGATAGAGAGCTGC
CGGAGGTCAAGCCGGCCATT

Downstream 100 bases:

>100_bases
GTCGTGTTGATCTTATGTTAGGATAGAGGGAATAATTTATAGAGGAAAGAGGATGAAGTTGGTTTTTTTAGATGAAAACA
AATGGCAGAAGATATTGGCT

Product: GAF/SpoIIE domain-containing protein

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MPAPAMPDRVAELEREHHRMQYLLAWLAFALRSFNNLNQILELTAFVASQLTDAEGGALVLFNPNGTIQLDQLHCSDLNH
REQIRAEMEQITQTLSAQEPILKTEGSLSVKIPALEHLLDERFRQSLGPDVRLFGTPLLIRNSVQGRLYVFSRQPGYHWD
ERRQQLLRLVADQAGVAIENDRLTAALRRKMALEKEMEIGSYIQTQLLPRECPQVKGIRLAARCRVASKVGGDYYDFIKI
PGERLETSDSSEETAGFRLAIVIGDVMGKGVPAGLLMTMTRAMVRAEALNRHSPARVLQHLNRAMYSDLENSNRFISLFY
SEYDPRSHRLCFSNAAHNPTLWWQARTREIQPLDTEGALIGLEENSHYSESCVQLQPGDVVVYYTDGYTETANTRGERLE
TSGLMDAIQYAARRYDSPEDILESLFEQMDAFRGRRGSHPLPLWLPEDPELLLQDEISLRPEPVDDTTLVVLKVLPDSEP
A

Sequences:

>Translated_481_residues
MPAPAMPDRVAELEREHHRMQYLLAWLAFALRSFNNLNQILELTAFVASQLTDAEGGALVLFNPNGTIQLDQLHCSDLNH
REQIRAEMEQITQTLSAQEPILKTEGSLSVKIPALEHLLDERFRQSLGPDVRLFGTPLLIRNSVQGRLYVFSRQPGYHWD
ERRQQLLRLVADQAGVAIENDRLTAALRRKMALEKEMEIGSYIQTQLLPRECPQVKGIRLAARCRVASKVGGDYYDFIKI
PGERLETSDSSEETAGFRLAIVIGDVMGKGVPAGLLMTMTRAMVRAEALNRHSPARVLQHLNRAMYSDLENSNRFISLFY
SEYDPRSHRLCFSNAAHNPTLWWQARTREIQPLDTEGALIGLEENSHYSESCVQLQPGDVVVYYTDGYTETANTRGERLE
TSGLMDAIQYAARRYDSPEDILESLFEQMDAFRGRRGSHPLPLWLPEDPELLLQDEISLRPEPVDDTTLVVLKVLPDSEP
A
>Mature_480_residues
PAPAMPDRVAELEREHHRMQYLLAWLAFALRSFNNLNQILELTAFVASQLTDAEGGALVLFNPNGTIQLDQLHCSDLNHR
EQIRAEMEQITQTLSAQEPILKTEGSLSVKIPALEHLLDERFRQSLGPDVRLFGTPLLIRNSVQGRLYVFSRQPGYHWDE
RRQQLLRLVADQAGVAIENDRLTAALRRKMALEKEMEIGSYIQTQLLPRECPQVKGIRLAARCRVASKVGGDYYDFIKIP
GERLETSDSSEETAGFRLAIVIGDVMGKGVPAGLLMTMTRAMVRAEALNRHSPARVLQHLNRAMYSDLENSNRFISLFYS
EYDPRSHRLCFSNAAHNPTLWWQARTREIQPLDTEGALIGLEENSHYSESCVQLQPGDVVVYYTDGYTETANTRGERLET
SGLMDAIQYAARRYDSPEDILESLFEQMDAFRGRRGSHPLPLWLPEDPELLLQDEISLRPEPVDDTTLVVLKVLPDSEPA

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 54524; Mature: 54392

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPAMPDRVAELEREHHRMQYLLAWLAFALRSFNNLNQILELTAFVASQLTDAEGGALV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LFNPNGTIQLDQLHCSDLNHREQIRAEMEQITQTLSAQEPILKTEGSLSVKIPALEHLLD
EECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECHHHHHHHH
ERFRQSLGPDVRLFGTPLLIRNSVQGRLYVFSRQPGYHWDERRQQLLRLVADQAGVAIEN
HHHHHHCCCCEEEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEC
DRLTAALRRKMALEKEMEIGSYIQTQLLPRECPQVKGIRLAARCRVASKVGGDYYDFIKI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEHHHHHHHHHHHCCCEEEHEEC
PGERLETSDSSEETAGFRLAIVIGDVMGKGVPAGLLMTMTRAMVRAEALNRHSPARVLQH
CCHHCCCCCCCCCCCCEEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LNRAMYSDLENSNRFISLFYSEYDPRSHRLCFSNAAHNPTLWWQARTREIQPLDTEGALI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCEE
GLEENSHYSESCVQLQPGDVVVYYTDGYTETANTRGERLETSGLMDAIQYAARRYDSPED
EEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
ILESLFEQMDAFRGRRGSHPLPLWLPEDPELLLQDEISLRPEPVDDTTLVVLKVLPDSEP
HHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHEECCCCCCCCCCCCCEEEEEEECCCCCC
A
C
>Mature Secondary Structure 
PAPAMPDRVAELEREHHRMQYLLAWLAFALRSFNNLNQILELTAFVASQLTDAEGGALV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LFNPNGTIQLDQLHCSDLNHREQIRAEMEQITQTLSAQEPILKTEGSLSVKIPALEHLLD
EECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECHHHHHHHH
ERFRQSLGPDVRLFGTPLLIRNSVQGRLYVFSRQPGYHWDERRQQLLRLVADQAGVAIEN
HHHHHHCCCCEEEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEC
DRLTAALRRKMALEKEMEIGSYIQTQLLPRECPQVKGIRLAARCRVASKVGGDYYDFIKI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEHHHHHHHHHHHCCCEEEHEEC
PGERLETSDSSEETAGFRLAIVIGDVMGKGVPAGLLMTMTRAMVRAEALNRHSPARVLQH
CCHHCCCCCCCCCCCCEEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LNRAMYSDLENSNRFISLFYSEYDPRSHRLCFSNAAHNPTLWWQARTREIQPLDTEGALI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCEE
GLEENSHYSESCVQLQPGDVVVYYTDGYTETANTRGERLETSGLMDAIQYAARRYDSPED
EEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
ILESLFEQMDAFRGRRGSHPLPLWLPEDPELLLQDEISLRPEPVDDTTLVVLKVLPDSEP
HHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHEECCCCCCCCCCCCCEEEEEEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]