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The map label for this gene is merA [H]

Identifier: 86609427

GI number: 86609427

Start: 2074827

End: 2076425

Strand: Direct

Name: merA [H]

Synonym: CYB_1977

Alternate gene names: 86609427

Gene position: 2074827-2076425 (Clockwise)

Preceding gene: 90296802

Following gene: 86609428

Centisome position: 68.1

GC content: 61.79

Gene sequence:

>1599_bases
GTGGAAAGGGATCCCCTTCTCCCATCCTGCCCCCGGCCCACTCAAGACATAGGATGGTGCTATCTTTTGCCGATTCCTGG
GATCCCTTGCCCGATTCTCCTGTTTAAAAGTTTAAAAAAAGCCATGCCTGTCTCCTACGATATTGTGGTCATTGGCGCCG
GTGCAGCGGGTTTGGTGGTGGCCAGCGCTGCGGCCCAACTGAAGGCCAAGGTGCTGCTGGTGGAAGGAAGCGACCGCCTG
GGCGGAGATTGCCTCTGGTATGGCTGTGTGCCCAGCAAAGCCCTTCTTCACGTGGCCCATACCGTCCATCGGATTCGCCA
GGCCATGGCTGCCGGTTGGGTAACTCTCCCTGGCCCAGCAGGGATCTCGGTGGATTACCTCAAGGTGTACGAACACATTC
GCTCTGCCCAGAGCTACATCGCCAACCACGCCGATTCGCCAGATCGCTTCCGCCAACTGGGGGTGGAACTGGTGTTTGCC
AAGGGCCACTTTGTGGATGGGCGCACCTTTGAGGTGGCAGGACGGCAGGTACAGGCGCGGGCTTTTGTCATCGCCACCGG
ATCCCGTCCTTGGGTTCCCCCCTTGCCGGGCTTGGCCGAGGCGGGCTATCTGACCAACGAATCGATCTTTGACTTGACCC
GCTTGCCCAAATCGGTGGCGGTGATAGGGGCGGGGCCGGTAGGCTGCGAGTTGAGCCAAGCTCTGGCCCGTCTGGGATCC
GAGGTGACCCTCATTGCCAGCCGCGAGCGCATCTTGCCCAAGGAGGATCCCGAAGCCGCCCAGGTGGTGCAGCAACAGTT
GACCCAAGATGGGATCCGCATCCTCACCCGGGTACGCGCCACAGCAGTTGGCCAAGAGCAAGGGGCAAAGCTGCTTTCCC
TAAAGGCCAATTCCGGGGCAGGCACCGCCGCTGCAGGTGATCAAGTGATCCGTGCAGAAGAGATTTTGGTGGCCGCCGGT
CGGATCCCGAACGTGGAAGGGCTGGGTCTAGAAGCCGCCGGCGTGCAGTACACCCCCCAAGGGATCCAGGTGAATGCCAA
GCTGCAAACCCGCAATCCCCGCATCTACGCCTGTGGGGATGTCATCGGCGGGCCGCAATTTACCCATGTGGCGGCCTACG
AAGGGGCAGTGGCCCTGGTCAATGCCCTGTTTTTCCCCCTCAGCCAAGCGCGTTATCGTGTCATTCCTTGGGCCATCTTC
ACCGAGCCGGAGCTGGCCCGTGTGGGCCTCACCGAAAGCGAAGCCCGGCAGCAATACGGCAAGGATGTGGTGGTGCTCAA
ACAGGAGTTTGCCGATGTGGATCGGGCCCAGGCAGAAGCCGCCCCCCTGGGCTTTGCCAAATTGATCTGTCGGCGCAACG
GCCAGATTTTGGGGGCGCACCTGGTGGGATCCCAGGCGGGAGAGCTGATTCATGAAGTGGTTTTGGCCATGAGCCGGCGG
CTGCCCGTCTCTGCCCTGACCGGGATCCACATCTACCCCACCCGCTCGGAGGTGAATGCCAAGGCGGCCCTGCAGCACCG
CAGGCAACAATTGGCCACCAACCCCCGCCTGCAGAACTTCTTGCAGGGATTCTTTGCCTGGAGGCGGGCCTGGAGCTGA

Upstream 100 bases:

>100_bases
AAGTCCCCAAACCCTGAAAAGTAGAACAGACGGATCAACCCAGCTTGCAAGGGATCCATCCGTCCCACTCTAACAAGTTC
TTCTCCCATGCAGAAAGGGT

Downstream 100 bases:

>100_bases
CAGCGCAAACTGGGGGTCAGCCACCGCTAGACTAGAATGGTCATTTACTGAGGGTTCCCGATGGGCTTTCTCCTGTCTGC
TGAACAAGTGGGTCAGTTTG

Product: pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MERDPLLPSCPRPTQDIGWCYLLPIPGIPCPILLFKSLKKAMPVSYDIVVIGAGAAGLVVASAAAQLKAKVLLVEGSDRL
GGDCLWYGCVPSKALLHVAHTVHRIRQAMAAGWVTLPGPAGISVDYLKVYEHIRSAQSYIANHADSPDRFRQLGVELVFA
KGHFVDGRTFEVAGRQVQARAFVIATGSRPWVPPLPGLAEAGYLTNESIFDLTRLPKSVAVIGAGPVGCELSQALARLGS
EVTLIASRERILPKEDPEAAQVVQQQLTQDGIRILTRVRATAVGQEQGAKLLSLKANSGAGTAAAGDQVIRAEEILVAAG
RIPNVEGLGLEAAGVQYTPQGIQVNAKLQTRNPRIYACGDVIGGPQFTHVAAYEGAVALVNALFFPLSQARYRVIPWAIF
TEPELARVGLTESEARQQYGKDVVVLKQEFADVDRAQAEAAPLGFAKLICRRNGQILGAHLVGSQAGELIHEVVLAMSRR
LPVSALTGIHIYPTRSEVNAKAALQHRRQQLATNPRLQNFLQGFFAWRRAWS

Sequences:

>Translated_532_residues
MERDPLLPSCPRPTQDIGWCYLLPIPGIPCPILLFKSLKKAMPVSYDIVVIGAGAAGLVVASAAAQLKAKVLLVEGSDRL
GGDCLWYGCVPSKALLHVAHTVHRIRQAMAAGWVTLPGPAGISVDYLKVYEHIRSAQSYIANHADSPDRFRQLGVELVFA
KGHFVDGRTFEVAGRQVQARAFVIATGSRPWVPPLPGLAEAGYLTNESIFDLTRLPKSVAVIGAGPVGCELSQALARLGS
EVTLIASRERILPKEDPEAAQVVQQQLTQDGIRILTRVRATAVGQEQGAKLLSLKANSGAGTAAAGDQVIRAEEILVAAG
RIPNVEGLGLEAAGVQYTPQGIQVNAKLQTRNPRIYACGDVIGGPQFTHVAAYEGAVALVNALFFPLSQARYRVIPWAIF
TEPELARVGLTESEARQQYGKDVVVLKQEFADVDRAQAEAAPLGFAKLICRRNGQILGAHLVGSQAGELIHEVVLAMSRR
LPVSALTGIHIYPTRSEVNAKAALQHRRQQLATNPRLQNFLQGFFAWRRAWS
>Mature_532_residues
MERDPLLPSCPRPTQDIGWCYLLPIPGIPCPILLFKSLKKAMPVSYDIVVIGAGAAGLVVASAAAQLKAKVLLVEGSDRL
GGDCLWYGCVPSKALLHVAHTVHRIRQAMAAGWVTLPGPAGISVDYLKVYEHIRSAQSYIANHADSPDRFRQLGVELVFA
KGHFVDGRTFEVAGRQVQARAFVIATGSRPWVPPLPGLAEAGYLTNESIFDLTRLPKSVAVIGAGPVGCELSQALARLGS
EVTLIASRERILPKEDPEAAQVVQQQLTQDGIRILTRVRATAVGQEQGAKLLSLKANSGAGTAAAGDQVIRAEEILVAAG
RIPNVEGLGLEAAGVQYTPQGIQVNAKLQTRNPRIYACGDVIGGPQFTHVAAYEGAVALVNALFFPLSQARYRVIPWAIF
TEPELARVGLTESEARQQYGKDVVVLKQEFADVDRAQAEAAPLGFAKLICRRNGQILGAHLVGSQAGELIHEVVLAMSRR
LPVSALTGIHIYPTRSEVNAKAALQHRRQQLATNPRLQNFLQGFFAWRRAWS

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=32, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=27.9569892473118, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI22035672, Length=501, Percent_Identity=30.1397205588822, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI291045266, Length=501, Percent_Identity=28.7425149700599, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=24.3362831858407, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=24.3362831858407, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=24.3362831858407, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=24.3362831858407, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=24.3362831858407, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI291045268, Length=495, Percent_Identity=27.6767676767677, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=30.2771855010661, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082354, Length=451, Percent_Identity=29.0465631929047, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=30.6209850107066, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=27.801724137931, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789065, Length=200, Percent_Identity=29.5, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=31.8565400843882, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17557007, Length=520, Percent_Identity=27.5, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=499, Percent_Identity=26.8537074148297, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=399, Percent_Identity=29.5739348370927, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=399, Percent_Identity=29.5739348370927, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=30.9426229508197, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=487, Percent_Identity=28.3367556468172, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=24.8421052631579, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=33.8297872340426, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=30.1030927835052, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24640551, Length=494, Percent_Identity=30.1619433198381, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=30.1030927835052, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=30.8641975308642, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 57293; Mature: 57293

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDPLLPSCPRPTQDIGWCYLLPIPGIPCPILLFKSLKKAMPVSYDIVVIGAGAAGLVV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHH
ASAAAQLKAKVLLVEGSDRLGGDCLWYGCVPSKALLHVAHTVHRIRQAMAAGWVTLPGPA
HHHHHHCEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCC
GISVDYLKVYEHIRSAQSYIANHADSPDRFRQLGVELVFAKGHFVDGRTFEVAGRQVQAR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCEEECCCEEEECCCEEEEE
AFVIATGSRPWVPPLPGLAEAGYLTNESIFDLTRLPKSVAVIGAGPVGCELSQALARLGS
EEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCC
EVTLIASRERILPKEDPEAAQVVQQQLTQDGIRILTRVRATAVGQEQGAKLLSLKANSGA
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCC
GTAAAGDQVIRAEEILVAAGRIPNVEGLGLEAAGVQYTPQGIQVNAKLQTRNPRIYACGD
CCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCEECCCCEEEEEEEEECCCEEEECCC
VIGGPQFTHVAAYEGAVALVNALFFPLSQARYRVIPWAIFTEPELARVGLTESEARQQYG
CCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCCHHHHHHHHC
KDVVVLKQEFADVDRAQAEAAPLGFAKLICRRNGQILGAHLVGSQAGELIHEVVLAMSRR
CCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCC
LPVSALTGIHIYPTRSEVNAKAALQHRRQQLATNPRLQNFLQGFFAWRRAWS
CCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERDPLLPSCPRPTQDIGWCYLLPIPGIPCPILLFKSLKKAMPVSYDIVVIGAGAAGLVV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHH
ASAAAQLKAKVLLVEGSDRLGGDCLWYGCVPSKALLHVAHTVHRIRQAMAAGWVTLPGPA
HHHHHHCEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCC
GISVDYLKVYEHIRSAQSYIANHADSPDRFRQLGVELVFAKGHFVDGRTFEVAGRQVQAR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCEEECCCEEEECCCEEEEE
AFVIATGSRPWVPPLPGLAEAGYLTNESIFDLTRLPKSVAVIGAGPVGCELSQALARLGS
EEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCC
EVTLIASRERILPKEDPEAAQVVQQQLTQDGIRILTRVRATAVGQEQGAKLLSLKANSGA
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCC
GTAAAGDQVIRAEEILVAAGRIPNVEGLGLEAAGVQYTPQGIQVNAKLQTRNPRIYACGD
CCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCEECCCCEEEEEEEEECCCEEEECCC
VIGGPQFTHVAAYEGAVALVNALFFPLSQARYRVIPWAIFTEPELARVGLTESEARQQYG
CCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCCHHHHHHHHC
KDVVVLKQEFADVDRAQAEAAPLGFAKLICRRNGQILGAHLVGSQAGELIHEVVLAMSRR
CCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCC
LPVSALTGIHIYPTRSEVNAKAALQHRRQQLATNPRLQNFLQGFFAWRRAWS
CCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]