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The map label for this gene is metE

Identifier: 86609342

GI number: 86609342

Start: 1971812

End: 1974076

Strand: Direct

Name: metE

Synonym: CYB_1886

Alternate gene names: 86609342

Gene position: 1971812-1974076 (Clockwise)

Preceding gene: 86609329

Following gene: 86609345

Centisome position: 64.72

GC content: 58.68

Gene sequence:

>2265_bases
ATGACTGCACAAACCATGACATTGGGGTATGCCCGCATGGGCAAGCGCCGCGAGCTCAAAAAAGCTTTGGAAGGCTTTTG
GAGTGGTGCCCTAGGGTCAGAGGCTCTGCTGGCTACCTTTTGGGATTTGGAGACTCAAGCCTGGCAGACTCAGCTACAAG
CTGGGATTGATCACATTGCGGTAGGGGATCAGACTCTCTACGACCATGTACTGGACTGGGCCACCTGGCTGGGGCTGATC
CCCTCTCGGTTCCGAGGTCTGTCGGGTTTAGACCGCTACTTTGCCATGGCGCGGGGGCGGGAGGGCCTGCCGGCTTTGGA
AATGACCAAGTGGTTTGACACCAACTACCACTACTTGGTACCCGAAATTGAGCCGGAAGCCGATCCCAGTCCTAACTTTG
GGGATTTTTTGGAAAGAGTTCGGCGGGCCCAAGGGATCCTAGGGGAGCGCACCAGCCCCGTTGTCCTCAGTCCAGTGACT
TTGCTCTGCCTTAGCCAGCGATCTGGGGATCTGCGGGCGGACTTGGAAAAGCTCCTTCCTCTGTACCGGGATCTCCTCCA
GGAGTTAAAACAGCTGGGGATCCCAGAAGTCCAGATTCACGATCCCATTTTGGTTACCAGCCAGGGAAGTGGCCTGCGGG
AAGCTGTGGAAATGAGCTACCGCCAGTTGGCAACCGCCGGGATCCCTGTCCACTTGGTCACCTACTTCGATGACCTGGGG
GAGACTTACCCCTGGGTGGTGCAACTGCCGGTGGCCGGCATTAGCCTTGACTTTACCCGTGGCCACACCCTGGATCTGGT
AAGAACCTATGGCTTTCCGGCTGACCAGATCCTAGGTGCTGGCGTAGTGGACGCTCGGAATGTCTGGAAGGTTCAGCCTG
AGACCGTCTTGGCCAGCCTGCGGGAGCTGCAGGGGGTAGCTCCCAACCTACGGGTGCAGCCTTCCGCTTCGTTGCAATTT
GTGCCCCACGACGCTGCTCTGGAAGCCCAGTTGCCGGAACCCCTCAGAAATGTCCTCAGCTTTGCCGAACAGAAGCTGGC
CGAAGTAGCTCTCCTGGCGCGAGCCCTCAACGGCGAAGACACTGCTGCCCAGCAGGCGGAAATCCAGCAGCAATGGCAGA
GCTTTGCGCAGTTTAGCCCCCCCAACCCCCAAGTGCGCCAGGCTTTGGCCAACCTCAAGCTCCAGGACTTCGAGCGATTC
CTGCCCTACGAGCAGCGCCTAAGTCGGCAGGTTCAGCTACCACCTTTGCCCACCACCACCATTGGCTCCTTCCCCCAAAC
TCCAGAGGTGCGGCAGTGGCGAGCTAAGTACAAAAGAGGGGAGGTTTCGCAGGCGGAGTACGAAGCAGCCATTGACGCTG
AGATTGCCAAGTGCATCCGCATTCAAGAAGAGATTGGCCTGGATGTGTTGGTGCATGGGGAATTTGAGCGCACCGACATG
GTGGAGTACTTTGCCCAGAAGCTGGAAGGCTTTGCCTTCACTGAACACGGCTGGGTGCAGAGTTACGGCAGCCGCTGCGT
GCGCCCTCCCATTCTCTACGGCGACGTGGTTCGCCCCCAACCCATGACAGTGCGGGAATTCCAAGTAGCCCAGTCCCACA
CCCAAAAGCCGGTGAAAGGCATGCTCACGGGGCCAGTGACTATGCTCAACTGGTCCTTTCCCCGTGTTGATATTCCCCGG
CGGGAGCAGGCTTTGCAGATTGCCCTGGCCTTGAGAGCCGAAGTGGCAGATCTGGAAGCGGCTGGAGCGGTGATGGTGCA
AGTGGATGAGCCGGCACTGCGGGAGGGGCTACCTCTGAAAAAAGAACGCTGGCCAGAATACCTCAGCTGGGCAGTGGATG
CCTTTCGCCTAGCTACCGGCGGGGCCAAGCCGGAAACCCAGATTCACACCCACATGTGCTACTCAGAGTTTGGGGACATC
ATCGAGCACATTGAGCGGCTGGATGCCGACGTGCTTTCCATTGAAAACAGCCGCAGCAACAACAAAACCCTGCTGCAAAT
TGCCCAGGCTGGGTATCGTCACCAGGTGGGCAACGGCGTTTACGATGTCCACAGCCCTGCGGTGCCGAGCGTTGAGCAAA
TCCTGCAGCAGCTGCGCACAGGACTGGCCCACTTGCCTGTGGAGCAAACTTGGGTTAATCCCGATTGTGGCCTCAAGACC
CGCCGCTGGGAGGAGGTGATCCCCGCACTGAAAAATATGGTGGCTGCCGCTCATCAGCTAGGGGAGGAGCTTCTGGAAAC
CCAACCTGAGGGTCTGCGCGAGTAA

Upstream 100 bases:

>100_bases
AGAGGAACAGCCGGTTTCTTCCTTGGCGGGCGGGATCCCAGTTGAGGGCGAATTGAACGCAGGAAAATTAGAAAACTCAG
TATTTGGGATGGCACACAAC

Downstream 100 bases:

>100_bases
GGCCCACGACCCGCTGATGTAACTGGTCGGGCTGGCTTGACAAGTCGCCGCGGGCGGTGCCGCTGTTGTGCTGATGCGGC
TGGCTTAGGGCCTACGGTCT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLI
PSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVT
LLCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIPVHLVTYFDDLG
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQF
VPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERF
LPYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPR
REQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
RRWEEVIPALKNMVAAAHQLGEELLETQPEGLRE

Sequences:

>Translated_754_residues
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLI
PSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVT
LLCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIPVHLVTYFDDLG
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQF
VPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERF
LPYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPR
REQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
RRWEEVIPALKNMVAAAHQLGEELLETQPEGLRE
>Mature_753_residues
TAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLIP
SRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTL
LCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIPVHLVTYFDDLGE
TYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQFV
PHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFL
PYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDMV
EYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPRR
EQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDII
EHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKTR
RWEEVIPALKNMVAAAHQLGEELLETQPEGLRE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=46.4993394980185, Blast_Score=613, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320936, Length=773, Percent_Identity=42.9495472186287, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_SYNJB (Q2JKE8)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478104.1
- ProteinModelPortal:   Q2JKE8
- SMR:   Q2JKE8
- STRING:   Q2JKE8
- GeneID:   3900829
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1886
- TIGR:   CYB_1886
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   TYLWLSK
- PhylomeDB:   Q2JKE8
- ProtClustDB:   PRK05222
- BioCyc:   SSP321332:CYB_1886-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 84678; Mature: 84546

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIA
CCCCEEEHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGDQTLYDHVLDWATWLGLIPSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTLLCLSQRSGDLRADLEKLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHHH
LYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIPVHLVTYFDDLG
HHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASL
CCCCEEEEECCCCEEEECCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCEECCHHHHHHHH
RELQGVAPNLRVQPSASLQFVPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGED
HHHHCCCCCCEECCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFLPYEQRLSRQVQLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCCCCCCCC
IGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKG
HHHHHHHHCCCEECCCHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHH
MLTGPVTMLNWSFPRVDIPRREQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
RRWEEVIPALKNMVAAAHQLGEELLETQPEGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIA
CCCEEEHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGDQTLYDHVLDWATWLGLIPSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTLLCLSQRSGDLRADLEKLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHHH
LYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIPVHLVTYFDDLG
HHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASL
CCCCEEEEECCCCEEEECCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCEECCHHHHHHHH
RELQGVAPNLRVQPSASLQFVPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGED
HHHHCCCCCCEECCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFLPYEQRLSRQVQLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCCCCCCCC
IGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKG
HHHHHHHHCCCEECCCHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHH
MLTGPVTMLNWSFPRVDIPRREQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
RRWEEVIPALKNMVAAAHQLGEELLETQPEGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA