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The map label for this gene is rne [H]

Identifier: 86609280

GI number: 86609280

Start: 1914255

End: 1916426

Strand: Direct

Name: rne [H]

Synonym: CYB_1823

Alternate gene names: 86609280

Gene position: 1914255-1916426 (Clockwise)

Preceding gene: 86609279

Following gene: 86609281

Centisome position: 62.83

GC content: 61.28

Gene sequence:

>2172_bases
ATGCCCAGGCAGATTGTCATTGCCGAGCAACATCGCGTGGCAGCCGTCTTCGCCGAAGATCAAGTGCAAGAGCTGATCGT
CGCCAGCGGCGCTCACCAAGTGGGAGACGTTTACGTCGGAATCATCGAGAACGTTCTGCCCAGCATCGATGCCGCTTTCG
TCAACATCGGCCCTGCAGAGCGCAATGGGTTTATCCACGTCAGCGACCTGGGCCCGCTGCGCCTCAAGCGAAGTCATGCC
TCGATTTCTGAGCTGGTCTTGCCGCAGCAAAAGGTGCTGGTACAGGTGATGAAAGAGCCTACCGGCAACAAAGGGCCAAG
GCTTACCGGAGATTTGGCCTTTCCAGGGCGGTATTTGGTGTTGCGACCCTACGGAAAAGGCGTTCATCTGTCCCGCCGCA
TCGAGGATCGAGAAGAACGCAACCGTTTGAAGTCGCTGGCCATTTTGATTAAACCCCCGCAGATGGGCCTGTTGGTGCGC
ACCGAAGCGGAAGGTGTGTCCGAAGAAGCGATGATCGACGACCTGGAGCAATTGAAGAAACTCTGGGAAGATATTCAACA
GCAGTATCAGGCAGCACGGGGGCCGGGGCTACTCAACCGGGATGACGACTTTATTCAGCGGGTGCTGCGGGATGTTTACT
CCGACGATGTGAACCGTATTGTGACCGACTCTGCGGCAGGAGCGAAGCGAGTCCGTAGCTATCTGCAGGGCTGGAACAAG
GGCCAAATGCCCCCCGGTGTCGTTGTCGATGCCCACCGCGAGCCCACCCCCATTCTGGAGTATTTTCGGGTCAACGCGGT
CATTCGGCAGGCGCTGCGGCCACGGGTGGATCTGCCCTCTGGTGGCTACATCATCATCGAGCCCACCGAGGCCCTGACGG
TAATTGATGTTAACTCGGGCTCCTTCACCCAATCGGCCACTTCTCGGGAGACAGTGCTGTGGACAAACTGTGAGGCGGCT
ACCGAGATTGCCCGCCAATTGAGGCTGCGCAACATTGCCGGTGTGATTGTGGTGGACTTCATCGATATGGATTCCCGCCG
CGACCAGTTGCTGGTTTTGGAGCATTTTAACAAGGCCCTCTCCAGCGATAAAGCTCGCCCACAGGTGGTCCAACTGTCGG
AATTGGGGCTGGTGGAGCTCACCCGCAAACGGCAGGGGCAAAGCCTTTACGAGATCTTCGGCTATTCTTGCCCCACCTGT
GAGGGGCTGGGGATCCTAGCCCGCTTGCCGGGGGCCGAGCCAGGGCATCTGTTAACAATAGAAAGCGGCCTGACGGTAAC
GCTACCCACCGAGGCGGAAGGAGGGGATGAGGAAGAGGCCGGCCCGATCCTGCCCCGCGATGGCAACCCCCAACGGGCAC
GCCTGCGAGTGGGGAGCGATGGGCTGTCTCGAGGCGATAGCCGTGCGCTGCTGCGGCGGTCGCGCTCTGCCCCCGGACAA
CGACGCCCCAGCCCGGATCCCTTTACGACTTCAGACGATCTGGAGGAAGAGCCACGGGTTGAAACGGGACGCAAGCGTCC
CCGTGAAGACTGGGGGGATCCCCGCACTGGCCAGCGCCTCGTTGCCCGGAGCCTGGATACTCCTGTAGAAGAGGAAGCCC
CAGAGCCGATGCAACTGCCAGGGGGCGGAGCAGACCTAAAGCGTCAAGAACCCCGCCGTTCTGGCCCTGGGGAAGAGGCA
GAATCCGCCTCACGCAAAGATCACACTTCCCCAGCCCGCACCGTTCGCGCCAGCGGGACGGAGTCCTCAGAAGGCCGTCC
GGCTCCGGTACGGCAAACCGTCACCGTGGAGATGACCCCGTTACAACAACGGGTCTACTCGGAGTTGGGGCTGTCGCCGC
TGTTGCTGCTTGACCAGCCGCCGCCTTGGGGCCGAGACACGGCGGTTAGGGTGGTTCTGCCGGGAACTCTCGAGGTGGAA
AGTAGCCCAGAGGTGGAGACTGAGGCAACTTCTCAGGCGGAGGAGTTGGCGCCCGAACCGGATGCCTTGCAGGAGAAGGA
GCCGCTGGATACCGTTTCTCCACCCGAACCCTCGGAACCCGAAATCTCCTCTGCTGAATCGTTCCCGTCAGCCGTGCAGA
GCAGCTTGGCCGAGCCAGCGGCGGAACTGGGGATCAGCCCTGCCCAGCAATCTCCAGCAGCCTCCAGGCGACGGCGCTCC
CGCAGCAGTTAG

Upstream 100 bases:

>100_bases
GGTTAAAGAAACTTGAGCAGATCCCAGCAAAAGGACAACACCTCGCCAGAACGAGGGTTCAATCCGTAACCGCGTGCCCT
TGGGGCGGGGAGGGAAGTTA

Downstream 100 bases:

>100_bases
GCGCTGCTCTCAAGCCCAGGGGCAGGCATGGGAGTATGGTGGAGCGGGCAAGGCCGAGTTTGATCTGGGAGGGATCCCTT
TGGCAGCAGGGGATCCTGCG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MPRQIVIAEQHRVAAVFAEDQVQELIVASGAHQVGDVYVGIIENVLPSIDAAFVNIGPAERNGFIHVSDLGPLRLKRSHA
SISELVLPQQKVLVQVMKEPTGNKGPRLTGDLAFPGRYLVLRPYGKGVHLSRRIEDREERNRLKSLAILIKPPQMGLLVR
TEAEGVSEEAMIDDLEQLKKLWEDIQQQYQAARGPGLLNRDDDFIQRVLRDVYSDDVNRIVTDSAAGAKRVRSYLQGWNK
GQMPPGVVVDAHREPTPILEYFRVNAVIRQALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTQSATSRETVLWTNCEAA
TEIARQLRLRNIAGVIVVDFIDMDSRRDQLLVLEHFNKALSSDKARPQVVQLSELGLVELTRKRQGQSLYEIFGYSCPTC
EGLGILARLPGAEPGHLLTIESGLTVTLPTEAEGGDEEEAGPILPRDGNPQRARLRVGSDGLSRGDSRALLRRSRSAPGQ
RRPSPDPFTTSDDLEEEPRVETGRKRPREDWGDPRTGQRLVARSLDTPVEEEAPEPMQLPGGGADLKRQEPRRSGPGEEA
ESASRKDHTSPARTVRASGTESSEGRPAPVRQTVTVEMTPLQQRVYSELGLSPLLLLDQPPPWGRDTAVRVVLPGTLEVE
SSPEVETEATSQAEELAPEPDALQEKEPLDTVSPPEPSEPEISSAESFPSAVQSSLAEPAAELGISPAQQSPAASRRRRS
RSS

Sequences:

>Translated_723_residues
MPRQIVIAEQHRVAAVFAEDQVQELIVASGAHQVGDVYVGIIENVLPSIDAAFVNIGPAERNGFIHVSDLGPLRLKRSHA
SISELVLPQQKVLVQVMKEPTGNKGPRLTGDLAFPGRYLVLRPYGKGVHLSRRIEDREERNRLKSLAILIKPPQMGLLVR
TEAEGVSEEAMIDDLEQLKKLWEDIQQQYQAARGPGLLNRDDDFIQRVLRDVYSDDVNRIVTDSAAGAKRVRSYLQGWNK
GQMPPGVVVDAHREPTPILEYFRVNAVIRQALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTQSATSRETVLWTNCEAA
TEIARQLRLRNIAGVIVVDFIDMDSRRDQLLVLEHFNKALSSDKARPQVVQLSELGLVELTRKRQGQSLYEIFGYSCPTC
EGLGILARLPGAEPGHLLTIESGLTVTLPTEAEGGDEEEAGPILPRDGNPQRARLRVGSDGLSRGDSRALLRRSRSAPGQ
RRPSPDPFTTSDDLEEEPRVETGRKRPREDWGDPRTGQRLVARSLDTPVEEEAPEPMQLPGGGADLKRQEPRRSGPGEEA
ESASRKDHTSPARTVRASGTESSEGRPAPVRQTVTVEMTPLQQRVYSELGLSPLLLLDQPPPWGRDTAVRVVLPGTLEVE
SSPEVETEATSQAEELAPEPDALQEKEPLDTVSPPEPSEPEISSAESFPSAVQSSLAEPAAELGISPAQQSPAASRRRRS
RSS
>Mature_722_residues
PRQIVIAEQHRVAAVFAEDQVQELIVASGAHQVGDVYVGIIENVLPSIDAAFVNIGPAERNGFIHVSDLGPLRLKRSHAS
ISELVLPQQKVLVQVMKEPTGNKGPRLTGDLAFPGRYLVLRPYGKGVHLSRRIEDREERNRLKSLAILIKPPQMGLLVRT
EAEGVSEEAMIDDLEQLKKLWEDIQQQYQAARGPGLLNRDDDFIQRVLRDVYSDDVNRIVTDSAAGAKRVRSYLQGWNKG
QMPPGVVVDAHREPTPILEYFRVNAVIRQALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTQSATSRETVLWTNCEAAT
EIARQLRLRNIAGVIVVDFIDMDSRRDQLLVLEHFNKALSSDKARPQVVQLSELGLVELTRKRQGQSLYEIFGYSCPTCE
GLGILARLPGAEPGHLLTIESGLTVTLPTEAEGGDEEEAGPILPRDGNPQRARLRVGSDGLSRGDSRALLRRSRSAPGQR
RPSPDPFTTSDDLEEEPRVETGRKRPREDWGDPRTGQRLVARSLDTPVEEEAPEPMQLPGGGADLKRQEPRRSGPGEEAE
SASRKDHTSPARTVRASGTESSEGRPAPVRQTVTVEMTPLQQRVYSELGLSPLLLLDQPPPWGRDTAVRVVLPGTLEVES
SPEVETEATSQAEELAPEPDALQEKEPLDTVSPPEPSEPEISSAESFPSAVQSSLAEPAAELGISPAQQSPAASRRRRSR
SS

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=418, Percent_Identity=35.8851674641148, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI87082235, Length=393, Percent_Identity=37.4045801526718, Blast_Score=228, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 79357; Mature: 79226

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQIVIAEQHRVAAVFAEDQVQELIVASGAHQVGDVYVGIIENVLPSIDAAFVNIGPAE
CCCEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCC
RNGFIHVSDLGPLRLKRSHASISELVLPQQKVLVQVMKEPTGNKGPRLTGDLAFPGRYLV
CCCEEEEECCCCHHEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
LRPYGKGVHLSRRIEDREERNRLKSLAILIKPPQMGLLVRTEAEGVSEEAMIDDLEQLKK
EECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LWEDIQQQYQAARGPGLLNRDDDFIQRVLRDVYSDDVNRIVTDSAAGAKRVRSYLQGWNK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GQMPPGVVVDAHREPTPILEYFRVNAVIRQALRPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCC
SFTQSATSRETVLWTNCEAATEIARQLRLRNIAGVIVVDFIDMDSRRDQLLVLEHFNKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEHHHHHHHHH
SSDKARPQVVQLSELGLVELTRKRQGQSLYEIFGYSCPTCEGLGILARLPGAEPGHLLTI
HCCCCCCCEEEHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHEECCCCCCCEEEEE
ESGLTVTLPTEAEGGDEEEAGPILPRDGNPQRARLRVGSDGLSRGDSRALLRRSRSAPGQ
ECCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCC
RRPSPDPFTTSDDLEEEPRVETGRKRPREDWGDPRTGQRLVARSLDTPVEEEAPEPMQLP
CCCCCCCCCCCCCCCCCCCHHHHCCCCCHHCCCCCHHHHHHHHHCCCCHHHCCCCCCCCC
GGGADLKRQEPRRSGPGEEAESASRKDHTSPARTVRASGTESSEGRPAPVRQTVTVEMTP
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCEEEEEEECH
LQQRVYSELGLSPLLLLDQPPPWGRDTAVRVVLPGTLEVESSPEVETEATSQAEELAPEP
HHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCHHHHHHHHHCCCCC
DALQEKEPLDTVSPPEPSEPEISSAESFPSAVQSSLAEPAAELGISPAQQSPAASRRRRS
CHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHC
RSS
CCC
>Mature Secondary Structure 
PRQIVIAEQHRVAAVFAEDQVQELIVASGAHQVGDVYVGIIENVLPSIDAAFVNIGPAE
CCEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCC
RNGFIHVSDLGPLRLKRSHASISELVLPQQKVLVQVMKEPTGNKGPRLTGDLAFPGRYLV
CCCEEEEECCCCHHEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
LRPYGKGVHLSRRIEDREERNRLKSLAILIKPPQMGLLVRTEAEGVSEEAMIDDLEQLKK
EECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LWEDIQQQYQAARGPGLLNRDDDFIQRVLRDVYSDDVNRIVTDSAAGAKRVRSYLQGWNK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GQMPPGVVVDAHREPTPILEYFRVNAVIRQALRPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCC
SFTQSATSRETVLWTNCEAATEIARQLRLRNIAGVIVVDFIDMDSRRDQLLVLEHFNKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEHHHHHHHHH
SSDKARPQVVQLSELGLVELTRKRQGQSLYEIFGYSCPTCEGLGILARLPGAEPGHLLTI
HCCCCCCCEEEHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHEECCCCCCCEEEEE
ESGLTVTLPTEAEGGDEEEAGPILPRDGNPQRARLRVGSDGLSRGDSRALLRRSRSAPGQ
ECCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCC
RRPSPDPFTTSDDLEEEPRVETGRKRPREDWGDPRTGQRLVARSLDTPVEEEAPEPMQLP
CCCCCCCCCCCCCCCCCCCHHHHCCCCCHHCCCCCHHHHHHHHHCCCCHHHCCCCCCCCC
GGGADLKRQEPRRSGPGEEAESASRKDHTSPARTVRASGTESSEGRPAPVRQTVTVEMTP
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCEEEEEEECH
LQQRVYSELGLSPLLLLDQPPPWGRDTAVRVVLPGTLEVESSPEVETEATSQAEELAPEP
HHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCHHHHHHHHHCCCCC
DALQEKEPLDTVSPPEPSEPEISSAESFPSAVQSSLAEPAAELGISPAQQSPAASRRRRS
CHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHC
RSS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]