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The map label for this gene is ndhF [H]

Identifier: 86609269

GI number: 86609269

Start: 1896359

End: 1898188

Strand: Reverse

Name: ndhF [H]

Synonym: CYB_1812

Alternate gene names: 86609269

Gene position: 1898188-1896359 (Counterclockwise)

Preceding gene: 86609270

Following gene: 86609268

Centisome position: 62.3

GC content: 61.69

Gene sequence:

>1830_bases
TTGGAGAGCAGTTGGTGGATCCCTTGCTATGGGTGGGCGGGAACAGCTTTGGCCATTCCCTGGGCAACGGGGTGGGTCAA
GGGCAGTGGCCCGCGCCCGGCAGCTTACCTCAACATCCTTCTGACTTTTGCCGCCTTTGCCCATGGCCTTTGGCTGTTGC
GGCAGGTTTGGGGTCGGGATCCCCAGATCCTGAACTACCCCTGGTTTCGGGTGGCCGATTTGGATTTGACTCTGTCTTTG
GAGATCTCGCCCCTGACAATGGGGGCCGTCGTCTGGATCAGCGGTTTGAGCCTGCTGGCCCAGATCTATGCGCTGGGGTA
TTTGGATAAGGACTGGGGCCTGGGTCGCTTTGCCGCCCTGATGGGCTTTTTCGAGGGGGGAATGACAGGGATCGCCCTCA
GCGACTCGCTGTTTTTCAGCTATGCCCTGCTGGAGATGCTCACCCTTTCCACCTATTTGCTGGTGGGCTTTTGGTACGCC
CAGCCTCTGGTGGTGACGGCGGCGCGGGATGCGTTTTTAACCAAGCGGGTAGGGGATCTGTTGCTGCTGATGGGGGTGGT
GGCAATTTCCAACTTTTCTGGGGCCTTGACCTTTTCCGGCCTGCGGGACTGGGCGGCTACGACTAGCTTGGATCCGGCCT
GGGCGGCTTTGATGGGGTTGGCTTTGATTGCAGGGCCTTTGGGGAAATGCGCCCAGTTTCCGCTGCACCTGTGGCTGGAT
GAGGCGATGGAAGCGCCCAACCCGGCTTCGATTTTGCGCAATTCGGCAGTGGTGGCCTGCGGGGCTTTTGTGCTGATTAA
GCTGCAACCGCTACTGGCCCTGTCGCCGATTACGAATGGGGTCTTGGTGGCCATCGGCACGATCACAGCCCTGGGCGGAT
CCCTGGTGGCCTTGGCGCAAATCGATATCAAACGCACCTTCTCCTACTCCACGAGTGCCTACTTGGGGTTGGTGTTTGTG
GCAGTGGGAATGCAGCAGCCGGAGCTGGCCCTGGAGTTGCTGCTCATGCACGGCCTGGCTAAGGCGCTGCTGTTTATGGG
GGTGGGATCCCTCATCTACACCACCGCTACCCAAAACCTGACGGAATTGGGAGGGTTGCGCAGCCGTATGCCGGCCACAG
TTGCCGCCTTTGCGGTGGGATCCCTGGGGATGGTGGCTCTGCTGCCCCTAGGGTGCTTTTGGACGCTGACCCGCCTGGCG
CTGGTCTACAGCGGGGTGCAGCCGGCCCTGGTGGGGGTGCTGCTGCTGGTGAATGGGCTGACGGCTCTCAACCTGGCGCG
GGTGTTTGGCCTGGTCTTTTTGGGATCCCCGCAACCCAAAACCCGCCGGGCCCCTGAGGTGGGCTGGCTGATGGCTCTGC
CCATGGGCATCCTGACGGTGCAAACCTTGCTCATGCCCTGGCTGTTGGGGCGCTGGGGGCTCTATCCCGACTGGAACCCG
CAGCTGAGGTGGATGGCGGCTCTGCTGACGGCTGCTGGGCTGGTGGGGGCAGCCATTGGCGGCTGGCTGTACTGGGGGGA
CCAACGGAGGGTATATCTGCCCTGGCCGGAGTTGCAGGATCTGCTGGCCCACGACTTTTACACGGGGCACCTCTACCAAG
CCCTGGTGGTGCAACCGGTCAGCGCCCTCTCCCGCGGATTGGAGTGGCTGGATCGCAACGGAGTGGACGGCTTGGTGAAC
GGGGTGAGCCTGGCCTTTTTCTTCAGCGGCGAAGCCCTCAAGTACACCCTGTCGGGGCGGATGCAACTCTATGTGCTGGT
GATTGTCATCGGGATGGTGTTGGTTACAGCTTGGCTACTGCGTTCGCCCCATTTGGGGTTGGCCTATTGA

Upstream 100 bases:

>100_bases
AATGGGGAGCAGGTCGTTAAAAGGCCCGACTTATTGCTGTTAAGTTTAGTTTTCCGTTTTTAAGTATAAGTATTTGATGG
CCTTGAACCTTCTTTTGGGG

Downstream 100 bases:

>100_bases
TGGGAGAGAGCCATGTTGAGCCTTTTGATTGGGATCCCGTTGTTGGGGAGCATCTTGATTGGGTTGATTCCCGAGCAGGC
GGGGAAGGGCTTGCACCGGG

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MESSWWIPCYGWAGTALAIPWATGWVKGSGPRPAAYLNILLTFAAFAHGLWLLRQVWGRDPQILNYPWFRVADLDLTLSL
EISPLTMGAVVWISGLSLLAQIYALGYLDKDWGLGRFAALMGFFEGGMTGIALSDSLFFSYALLEMLTLSTYLLVGFWYA
QPLVVTAARDAFLTKRVGDLLLLMGVVAISNFSGALTFSGLRDWAATTSLDPAWAALMGLALIAGPLGKCAQFPLHLWLD
EAMEAPNPASILRNSAVVACGAFVLIKLQPLLALSPITNGVLVAIGTITALGGSLVALAQIDIKRTFSYSTSAYLGLVFV
AVGMQQPELALELLLMHGLAKALLFMGVGSLIYTTATQNLTELGGLRSRMPATVAAFAVGSLGMVALLPLGCFWTLTRLA
LVYSGVQPALVGVLLLVNGLTALNLARVFGLVFLGSPQPKTRRAPEVGWLMALPMGILTVQTLLMPWLLGRWGLYPDWNP
QLRWMAALLTAAGLVGAAIGGWLYWGDQRRVYLPWPELQDLLAHDFYTGHLYQALVVQPVSALSRGLEWLDRNGVDGLVN
GVSLAFFFSGEALKYTLSGRMQLYVLVIVIGMVLVTAWLLRSPHLGLAY

Sequences:

>Translated_609_residues
MESSWWIPCYGWAGTALAIPWATGWVKGSGPRPAAYLNILLTFAAFAHGLWLLRQVWGRDPQILNYPWFRVADLDLTLSL
EISPLTMGAVVWISGLSLLAQIYALGYLDKDWGLGRFAALMGFFEGGMTGIALSDSLFFSYALLEMLTLSTYLLVGFWYA
QPLVVTAARDAFLTKRVGDLLLLMGVVAISNFSGALTFSGLRDWAATTSLDPAWAALMGLALIAGPLGKCAQFPLHLWLD
EAMEAPNPASILRNSAVVACGAFVLIKLQPLLALSPITNGVLVAIGTITALGGSLVALAQIDIKRTFSYSTSAYLGLVFV
AVGMQQPELALELLLMHGLAKALLFMGVGSLIYTTATQNLTELGGLRSRMPATVAAFAVGSLGMVALLPLGCFWTLTRLA
LVYSGVQPALVGVLLLVNGLTALNLARVFGLVFLGSPQPKTRRAPEVGWLMALPMGILTVQTLLMPWLLGRWGLYPDWNP
QLRWMAALLTAAGLVGAAIGGWLYWGDQRRVYLPWPELQDLLAHDFYTGHLYQALVVQPVSALSRGLEWLDRNGVDGLVN
GVSLAFFFSGEALKYTLSGRMQLYVLVIVIGMVLVTAWLLRSPHLGLAY
>Mature_609_residues
MESSWWIPCYGWAGTALAIPWATGWVKGSGPRPAAYLNILLTFAAFAHGLWLLRQVWGRDPQILNYPWFRVADLDLTLSL
EISPLTMGAVVWISGLSLLAQIYALGYLDKDWGLGRFAALMGFFEGGMTGIALSDSLFFSYALLEMLTLSTYLLVGFWYA
QPLVVTAARDAFLTKRVGDLLLLMGVVAISNFSGALTFSGLRDWAATTSLDPAWAALMGLALIAGPLGKCAQFPLHLWLD
EAMEAPNPASILRNSAVVACGAFVLIKLQPLLALSPITNGVLVAIGTITALGGSLVALAQIDIKRTFSYSTSAYLGLVFV
AVGMQQPELALELLLMHGLAKALLFMGVGSLIYTTATQNLTELGGLRSRMPATVAAFAVGSLGMVALLPLGCFWTLTRLA
LVYSGVQPALVGVLLLVNGLTALNLARVFGLVFLGSPQPKTRRAPEVGWLMALPMGILTVQTLLMPWLLGRWGLYPDWNP
QLRWMAALLTAAGLVGAAIGGWLYWGDQRRVYLPWPELQDLLAHDFYTGHLYQALVVQPVSALSRGLEWLDRNGVDGLVN
GVSLAFFFSGEALKYTLSGRMQLYVLVIVIGMVLVTAWLLRSPHLGLAY

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=380, Percent_Identity=29.2105263157895, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1788614, Length=551, Percent_Identity=29.4010889292196, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1788827, Length=434, Percent_Identity=32.4884792626728, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=29.1878172588832, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788829, Length=414, Percent_Identity=27.0531400966184, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788613, Length=340, Percent_Identity=27.0588235294118, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI2367154, Length=218, Percent_Identity=31.1926605504587, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 65869; Mature: 65869

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSWWIPCYGWAGTALAIPWATGWVKGSGPRPAAYLNILLTFAAFAHGLWLLRQVWGRD
CCCCEEEEEECCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQILNYPWFRVADLDLTLSLEISPLTMGAVVWISGLSLLAQIYALGYLDKDWGLGRFAAL
CCEECCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MGFFEGGMTGIALSDSLFFSYALLEMLTLSTYLLVGFWYAQPLVVTAARDAFLTKRVGDL
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHH
LLLMGVVAISNFSGALTFSGLRDWAATTSLDPAWAALMGLALIAGPLGKCAQFPLHLWLD
HHHHHHHHHHCCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHH
EAMEAPNPASILRNSAVVACGAFVLIKLQPLLALSPITNGVLVAIGTITALGGSLVALAQ
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEHHH
IDIKRTFSYSTSAYLGLVFVAVGMQQPELALELLLMHGLAKALLFMGVGSLIYTTATQNL
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELGGLRSRMPATVAAFAVGSLGMVALLPLGCFWTLTRLALVYSGVQPALVGVLLLVNGL
HHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TALNLARVFGLVFLGSPQPKTRRAPEVGWLMALPMGILTVQTLLMPWLLGRWGLYPDWNP
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QLRWMAALLTAAGLVGAAIGGWLYWGDQRRVYLPWPELQDLLAHDFYTGHLYQALVVQPV
HHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
SALSRGLEWLDRNGVDGLVNGVSLAFFFSGEALKYTLSGRMQLYVLVIVIGMVLVTAWLL
HHHHHHHHHHHCCCCCHHHHHHEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
RSPHLGLAY
CCCCCCCCC
>Mature Secondary Structure
MESSWWIPCYGWAGTALAIPWATGWVKGSGPRPAAYLNILLTFAAFAHGLWLLRQVWGRD
CCCCEEEEEECCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQILNYPWFRVADLDLTLSLEISPLTMGAVVWISGLSLLAQIYALGYLDKDWGLGRFAAL
CCEECCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MGFFEGGMTGIALSDSLFFSYALLEMLTLSTYLLVGFWYAQPLVVTAARDAFLTKRVGDL
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHH
LLLMGVVAISNFSGALTFSGLRDWAATTSLDPAWAALMGLALIAGPLGKCAQFPLHLWLD
HHHHHHHHHHCCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHH
EAMEAPNPASILRNSAVVACGAFVLIKLQPLLALSPITNGVLVAIGTITALGGSLVALAQ
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEHHH
IDIKRTFSYSTSAYLGLVFVAVGMQQPELALELLLMHGLAKALLFMGVGSLIYTTATQNL
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELGGLRSRMPATVAAFAVGSLGMVALLPLGCFWTLTRLALVYSGVQPALVGVLLLVNGL
HHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TALNLARVFGLVFLGSPQPKTRRAPEVGWLMALPMGILTVQTLLMPWLLGRWGLYPDWNP
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QLRWMAALLTAAGLVGAAIGGWLYWGDQRRVYLPWPELQDLLAHDFYTGHLYQALVVQPV
HHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
SALSRGLEWLDRNGVDGLVNGVSLAFFFSGEALKYTLSGRMQLYVLVIVIGMVLVTAWLL
HHHHHHHHHHHCCCCCHHHHHHEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
RSPHLGLAY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]