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The map label for this gene is rph

Identifier: 86609199

GI number: 86609199

Start: 1823994

End: 1824728

Strand: Reverse

Name: rph

Synonym: CYB_1741

Alternate gene names: 86609199

Gene position: 1824728-1823994 (Counterclockwise)

Preceding gene: 86609200

Following gene: 86609198

Centisome position: 59.89

GC content: 59.18

Gene sequence:

>735_bases
ATGAGCTGGCAACGCCCAGATAATCGCACCGCTGCTCAATTGCGCCCGGTTTCTTTTGAGCGCCACTTTACCCGCTATGC
CCCTGGCTCGGTGTTGGTCAAGTTCGGCCATACCCATGTCCTTTGCACCGCCAGTGTGGCCGAAGAAGTTCCCCCTTTTC
TGCAGAACACCGGCCAAGGCTGGCTGACGGCTGAGTACCGCATGTTGCCCGGAGCCACGCAACAACGACAGCCGCGGGAA
ATGTTGAAACTATCGGGCCGTACTGCCGAGATTCAACGGCTCATCGGGCGCAGTCTGCGCGCCGCCTTGGATTTTCGCAA
GCTGGGATCCCGTACCATCACCGTCGATGCCGATGTGCTGCAGGCCGATGGCGGCACTCGCACCGCCGCGATCACAGGGG
GCTATGTGGCTCTGCACGACGCCATCACTTGGCTGTACAAACAGGGGGCGCTGGATCCGGCCCAGGGATCCCCGCTGCGG
CAGCAGGTGGCGGCCCTTTCAGTAGGAATTGTGCAGGGAGAGGTACTTGTGGATCTGTGCTATGAGGAAGACAGCCAAGC
CGAGGTGGACATGAACATTGTTATGAACGAGCAGGGATCCTTCATCGAGATCCAGGGCACTGCCGAAGCCGCCTGCTTCA
GTCGCCCGCAACTGCTACAAATGTTGGAGATGGCCCAAGCCGGGATCCAAGAATTGCTACAAGCCCAACGGCAGGCTCTC
AATTTAGAAAGATAG

Upstream 100 bases:

>100_bases
CGCAGCCATCGCCCCAGTCATGTTCTGCAAAGATTCCAAGTCCTGAGCCGCATTGGGCAGACATGGTTGACAATGCGAGG
GATCCCTTTTTGAACTGTCC

Downstream 100 bases:

>100_bases
AGCCATAGCCGGACCCTAGCCTTGCAATGGTAATCCTTCAAGGCACTGCATATGGGATTGCATGGGTGTATATCCTGGTT
GGCAAAGCGGTATTCCCTTG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSWQRPDNRTAAQLRPVSFERHFTRYAPGSVLVKFGHTHVLCTASVAEEVPPFLQNTGQGWLTAEYRMLPGATQQRQPRE
MLKLSGRTAEIQRLIGRSLRAALDFRKLGSRTITVDADVLQADGGTRTAAITGGYVALHDAITWLYKQGALDPAQGSPLR
QQVAALSVGIVQGEVLVDLCYEEDSQAEVDMNIVMNEQGSFIEIQGTAEAACFSRPQLLQMLEMAQAGIQELLQAQRQAL
NLER

Sequences:

>Translated_244_residues
MSWQRPDNRTAAQLRPVSFERHFTRYAPGSVLVKFGHTHVLCTASVAEEVPPFLQNTGQGWLTAEYRMLPGATQQRQPRE
MLKLSGRTAEIQRLIGRSLRAALDFRKLGSRTITVDADVLQADGGTRTAAITGGYVALHDAITWLYKQGALDPAQGSPLR
QQVAALSVGIVQGEVLVDLCYEEDSQAEVDMNIVMNEQGSFIEIQGTAEAACFSRPQLLQMLEMAQAGIQELLQAQRQAL
NLER
>Mature_243_residues
SWQRPDNRTAAQLRPVSFERHFTRYAPGSVLVKFGHTHVLCTASVAEEVPPFLQNTGQGWLTAEYRMLPGATQQRQPREM
LKLSGRTAEIQRLIGRSLRAALDFRKLGSRTITVDADVLQADGGTRTAAITGGYVALHDAITWLYKQGALDPAQGSPLRQ
QVAALSVGIVQGEVLVDLCYEEDSQAEVDMNIVMNEQGSFIEIQGTAEAACFSRPQLLQMLEMAQAGIQELLQAQRQALN
LER

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=58.0188679245283, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SYNJB (Q2JKT3)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477961.1
- ProteinModelPortal:   Q2JKT3
- SMR:   Q2JKT3
- STRING:   Q2JKT3
- GeneID:   3901803
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1741
- TIGR:   CYB_1741
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q2JKT3
- ProtClustDB:   CLSK750722
- BioCyc:   SSP321332:CYB_1741-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26922; Mature: 26791

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQRPDNRTAAQLRPVSFERHFTRYAPGSVLVKFGHTHVLCTASVAEEVPPFLQNTGQG
CCCCCCCCCCHHHCCCCCHHHHHHHCCCCEEEEEECCEEEEEEHHHHHHCCHHHHCCCCC
WLTAEYRMLPGATQQRQPREMLKLSGRTAEIQRLIGRSLRAALDFRKLGSRTITVDADVL
EEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHEE
QADGGTRTAAITGGYVALHDAITWLYKQGALDPAQGSPLRQQVAALSVGIVQGEVLVDLC
ECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
YEEDSQAEVDMNIVMNEQGSFIEIQGTAEAACFSRPQLLQMLEMAQAGIQELLQAQRQAL
CCCCCCCEEEEEEEECCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NLER
CCCC
>Mature Secondary Structure 
SWQRPDNRTAAQLRPVSFERHFTRYAPGSVLVKFGHTHVLCTASVAEEVPPFLQNTGQG
CCCCCCCCCHHHCCCCCHHHHHHHCCCCEEEEEECCEEEEEEHHHHHHCCHHHHCCCCC
WLTAEYRMLPGATQQRQPREMLKLSGRTAEIQRLIGRSLRAALDFRKLGSRTITVDADVL
EEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHEE
QADGGTRTAAITGGYVALHDAITWLYKQGALDPAQGSPLRQQVAALSVGIVQGEVLVDLC
ECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
YEEDSQAEVDMNIVMNEQGSFIEIQGTAEAACFSRPQLLQMLEMAQAGIQELLQAQRQAL
CCCCCCCEEEEEEEECCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NLER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA