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The map label for this gene is leuB [H]

Identifier: 86609093

GI number: 86609093

Start: 1701592

End: 1702707

Strand: Direct

Name: leuB [H]

Synonym: CYB_1631

Alternate gene names: 86609093

Gene position: 1701592-1702707 (Clockwise)

Preceding gene: 86609092

Following gene: 86609099

Centisome position: 55.85

GC content: 61.2

Gene sequence:

>1116_bases
ATGAGCACTTCTTCTTCAACTGCTCGAACCTATCGGATCACAGCCCTTGCCGGAGATGGCATCGGCCCCGAAATCATGCG
GGTGGGACGAGCCGTTTTGGATGCAGTTGCCCGGCAGATGGGATTTGCTCTTCAGTGGCAAGAGGGCCTGATCGGCGGGG
CCGCCTACGACCAGACCGGGGATCCCTTGCCGGCAGAAACCCTGAAAATGGCTCAAGAGAGCGATGCCGTTTATCTGGCT
GCTGTCGGCGACTTCAAGTATGACGCTCTGCCTCCTGAGAAACGCCCGGAGCGGGCCTTGTTGGGCTTGAGGGCGGGGCT
GGGGCTGTTTGCCAACTTGCGCCCGGTGAAGGTCTTCCCTCAACTGGTGGGGGCCTCCTCCCTGAAGCCGGAGGTGATCG
CGGGGATCGACCTGGTGGTAGTGCGGGAGCTGACGGGGGGCATTTACTTCGGCCAGCCCAAGGGAATCTTCACGGATGCC
CAGGGATCTCGGCGCGGCGTGAACACCATGGTCTATAGCGAAGCTGAGGTGGATCGGATTGCCCGAGTTGCCTTTGAGCT
GGCCCGCAAACGCCGGCGCAAGCTCTGCTCGGTGGACAAAGCCAACGTTCTCGAGGTGTCGCAACTGTGGCGGGAGCGGG
TCAATGCCATTGCGGCTGAGTACCCGGAGGTGGAACTTTCCCACCTGTACATCGACAATGCCGCCATGCAGTTGGTGCGC
TGGCCCAAGCAGTTCGACGTCATCCTCACGGGCAATCTCTTCGGCGATATCCTCTCCGATGAAGCGGCCATGCTGACCGG
CTCTATTGGTATGCTGCCCTCTGCCTCTCTGGGATCCTCTGGCCCCGGCCTATACGAGCCGGTACATGGGTCGGCTCCCG
ACATTGCCGGACAAGACAAAGCCAACCCCATTGCCCAGGTGCTCTCCGGCGCCCTGATGCTCCGCTACTCCCTGGATCAG
CCGCAAGCTGCCGACCGCATCGAACAGGCGGTAGAAGCCGTTTTGGCGCAAGGGTACCGCACCCCAGACCTCTACTCTGA
GGGAATGACTTTAGTGGGTTGCCAGGAAATGGGAGAAAAGTTGGTGGCAGCCCTGTCCCAGCAGCAGGCGCAATAG

Upstream 100 bases:

>100_bases
AGCTTTTTGGAGAAGTTGTCTGCAAACCTGTGTGCCAAGGGATGGGCAAGTCCGCCGAAATGGTAGTATCTCTACGCCCT
CATCTTCAGCTTGACCGCTC

Downstream 100 bases:

>100_bases
CCTTTTTCGGGGCCACAGGCTACAGCCAAGGCAGACGTGCGGCGATTTCCAAGCAATTTCCAATTGTGGGGATCCTTGGA
GCTACGCCCAACCGGCTTCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSTSSSTARTYRITALAGDGIGPEIMRVGRAVLDAVARQMGFALQWQEGLIGGAAYDQTGDPLPAETLKMAQESDAVYLA
AVGDFKYDALPPEKRPERALLGLRAGLGLFANLRPVKVFPQLVGASSLKPEVIAGIDLVVVRELTGGIYFGQPKGIFTDA
QGSRRGVNTMVYSEAEVDRIARVAFELARKRRRKLCSVDKANVLEVSQLWRERVNAIAAEYPEVELSHLYIDNAAMQLVR
WPKQFDVILTGNLFGDILSDEAAMLTGSIGMLPSASLGSSGPGLYEPVHGSAPDIAGQDKANPIAQVLSGALMLRYSLDQ
PQAADRIEQAVEAVLAQGYRTPDLYSEGMTLVGCQEMGEKLVAALSQQQAQ

Sequences:

>Translated_371_residues
MSTSSSTARTYRITALAGDGIGPEIMRVGRAVLDAVARQMGFALQWQEGLIGGAAYDQTGDPLPAETLKMAQESDAVYLA
AVGDFKYDALPPEKRPERALLGLRAGLGLFANLRPVKVFPQLVGASSLKPEVIAGIDLVVVRELTGGIYFGQPKGIFTDA
QGSRRGVNTMVYSEAEVDRIARVAFELARKRRRKLCSVDKANVLEVSQLWRERVNAIAAEYPEVELSHLYIDNAAMQLVR
WPKQFDVILTGNLFGDILSDEAAMLTGSIGMLPSASLGSSGPGLYEPVHGSAPDIAGQDKANPIAQVLSGALMLRYSLDQ
PQAADRIEQAVEAVLAQGYRTPDLYSEGMTLVGCQEMGEKLVAALSQQQAQ
>Mature_370_residues
STSSSTARTYRITALAGDGIGPEIMRVGRAVLDAVARQMGFALQWQEGLIGGAAYDQTGDPLPAETLKMAQESDAVYLAA
VGDFKYDALPPEKRPERALLGLRAGLGLFANLRPVKVFPQLVGASSLKPEVIAGIDLVVVRELTGGIYFGQPKGIFTDAQ
GSRRGVNTMVYSEAEVDRIARVAFELARKRRRKLCSVDKANVLEVSQLWRERVNAIAAEYPEVELSHLYIDNAAMQLVRW
PKQFDVILTGNLFGDILSDEAAMLTGSIGMLPSASLGSSGPGLYEPVHGSAPDIAGQDKANPIAQVLSGALMLRYSLDQP
QAADRIEQAVEAVLAQGYRTPDLYSEGMTLVGCQEMGEKLVAALSQQQAQ

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=346, Percent_Identity=33.5260115606936, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4758582, Length=350, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=28.6153846153846, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=366, Percent_Identity=28.6885245901639, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=30.6709265175719, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178819, Length=167, Percent_Identity=32.9341317365269, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=54.8022598870056, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788101, Length=351, Percent_Identity=39.6011396011396, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=28.927680798005, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71986051, Length=357, Percent_Identity=33.8935574229692, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=309, Percent_Identity=32.0388349514563, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=337, Percent_Identity=27.893175074184, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=28.6549707602339, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=344, Percent_Identity=44.7674418604651, Blast_Score=248, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=36.5921787709497, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=32.4712643678161, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=371, Percent_Identity=31.5363881401617, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24643268, Length=369, Percent_Identity=33.0623306233062, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24643270, Length=369, Percent_Identity=33.0623306233062, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=29.9450549450549, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=28.9085545722714, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=29.0419161676647, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281362242, Length=258, Percent_Identity=30.6201550387597, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648872, Length=258, Percent_Identity=30.6201550387597, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130355, Length=346, Percent_Identity=24.5664739884393, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39897; Mature: 39766

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSSTARTYRITALAGDGIGPEIMRVGRAVLDAVARQMGFALQWQEGLIGGAAYDQTG
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
DPLPAETLKMAQESDAVYLAAVGDFKYDALPPEKRPERALLGLRAGLGLFANLRPVKVFP
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
QLVGASSLKPEVIAGIDLVVVRELTGGIYFGQPKGIFTDAQGSRRGVNTMVYSEAEVDRI
HHHCCCCCCCHHHHCCHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCEEEECHHHHHHH
ARVAFELARKRRRKLCSVDKANVLEVSQLWRERVNAIAAEYPEVELSHLYIDNAAMQLVR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
WPKQFDVILTGNLFGDILSDEAAMLTGSIGMLPSASLGSSGPGLYEPVHGSAPDIAGQDK
CCCCCCEEEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANPIAQVLSGALMLRYSLDQPQAADRIEQAVEAVLAQGYRTPDLYSEGMTLVGCQEMGEK
CCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEEHHHHHHH
LVAALSQQQAQ
HHHHHHHHCCC
>Mature Secondary Structure 
STSSSTARTYRITALAGDGIGPEIMRVGRAVLDAVARQMGFALQWQEGLIGGAAYDQTG
CCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
DPLPAETLKMAQESDAVYLAAVGDFKYDALPPEKRPERALLGLRAGLGLFANLRPVKVFP
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
QLVGASSLKPEVIAGIDLVVVRELTGGIYFGQPKGIFTDAQGSRRGVNTMVYSEAEVDRI
HHHCCCCCCCHHHHCCHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCEEEECHHHHHHH
ARVAFELARKRRRKLCSVDKANVLEVSQLWRERVNAIAAEYPEVELSHLYIDNAAMQLVR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
WPKQFDVILTGNLFGDILSDEAAMLTGSIGMLPSASLGSSGPGLYEPVHGSAPDIAGQDK
CCCCCCEEEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANPIAQVLSGALMLRYSLDQPQAADRIEQAVEAVLAQGYRTPDLYSEGMTLVGCQEMGEK
CCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEEHHHHHHH
LVAALSQQQAQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA