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The map label for this gene is pyk [H]

Identifier: 86609010

GI number: 86609010

Start: 1620132

End: 1621991

Strand: Reverse

Name: pyk [H]

Synonym: CYB_1545

Alternate gene names: 86609010

Gene position: 1621991-1620132 (Counterclockwise)

Preceding gene: 86609012

Following gene: 86609009

Centisome position: 53.24

GC content: 59.84

Gene sequence:

>1860_bases
ATGCATTTGAGGGATTCTTTTCGACGCACCAAAATTGTTGCCACCATCGGCCCTGCCAGCTCAAATCCAGCCATTCTCAG
GGAGATGATCCTGCAAGGGGCAACCACCCTTCGCCTCAACTTCTCCCATGGGGATCACGAGCTACACCGCCGCAGCATTC
GCCTGATCCGCCAAACCGCGATGGATCTGGGCATCCAGGTCGCCATTTTGCAAGACCTGCAGGGGCCCAAAATCCGCCTG
GGCAAGTTCGCCGAAGGCTCGATCACCCTCAAGGCAGGGGATCCCTTTGTGTTGACCAGCAAACCTGTCTTGGGATCCCA
AGAGCGCAGTTGGGTCACCTACGACAAGCTGGCCCAAGAAGTGCCCGAAGGAGCGACGATCCTCATCGACGATGGTCGAG
TGGAGATGCGGGTGGAAGCCGTGGATCCGGAAGCGGGCGAACTCTTCTGCCGCACCATTGTCGGGGGTACCCTCTCCAAC
AACAAGGGGGTCAATTTCCCCGGTGTGCGCCTGAGCATCCGCGCCGTGACCCCAAAAGACAAAGAAGATCTCTACTTCGG
CCTCAACCAAGGGGTGGATTGGGTGGCCCTCAGCTTTGTGCGGGATCCCTCTGATGTGCTGGAGCTGCGGGAGCTCATCG
CCTCTGCCAAACGGCCTTCTTCGGGCTCAGCCGACAAGCGGGTACCCATCATCGTCAAGATCGAGAAACACGAGGCCATT
GAACAGCTGCCCCAGATTTTGGCCCTCAGCGACGGTGTGATGGTGGCGCGGGGAGATCTGGGGGTAGAGCTGCCTGCGGA
AGAGGTGCCGATCCTGCAGAAGCGGGTGATCGCCCTGGCCAACTCCCTGGGGATCCCGGTAATTACGGCCACCCAAATGC
TGGACAGCATGGTGCACAGCCCCCGGCCTACCCGTGCCGAAATCTCCGATGTGGCCAACGCCATTTTGGATGGCACCGAT
GCCGTCATGCTCTCCAACGAGACGGCTGTGGGCAAATACCCTGTCGAGGCCGTTGCCACCATGGCCCGCATTGCCGTGCG
CACCGAGCGCGATTACTTTGACCTGACCTACGCCGACCGACGACAACGCCTTCGCGCCCTTGCCATGCAGGAGCTGAGCC
AAAACAACGGCAACAGTCCTGCCGGGATGCGGCCCAAAACCCTGATCACCGACTCGATCAGCCGCGCCGTGGGGGAGATC
GCCCAAGAGTTGGATGCTGTAGCCGTGATGACCCTGACCAAAACAGGGGCTACCGCTCGCAATGTCTCTAAGTTTCGCCC
CCGCACTCCCATCCTAGCCGTAACCCCCCATGTGGAAGTGGCACGGCGGCTGCAGTTGGTTTGGGGGGTACATCCGCTGT
TGGTTATGGATTTGGCCACCACCCGCCAGACCTTTCAAGCGGCCATCAGCTTGGCTCAGGAAGATGGCTTGCTCAAGGAT
GGCGACTTGGTGGTGCTCAGTGCAGGAACCCTGCCAGGCGTTGCGGGATCCACCGATCTGATCAAGGTGGATGTGGTGAA
AGCGGTGGTGGCTCAAGGCAAAGGGTTTGGCAAAGGCATTGTCAGCGGCCCGGCCCGCATCTTGAGATCCAGCCTCGATG
CCAGCAAAATCACCGCCGGAGACATTTTGGTTGCCCAATCCACCGACGCCAACTATGTGGAGGCCATCAAACAGGCAGCC
GGCGTGATTACCGAAGAAGAGGGAGCCTCCTCTCATGCAGCAGTCATTGGCCTGCGGCTGGGGGTGCCGGTGCTGGTGGG
GGTCAAGGATGCTACCCGCTTAATCCGGGATGGATCTATCGTCACTCTGAACCCAGAAAAAGGCATAGTGACCGCAGGAG
CCGATGGCTTGGGCTTTTAA

Upstream 100 bases:

>100_bases
AAAAAGCGGTGCCTCAAAAAAGACATAACGGGTTATCGTAATCAGAGAGATCCCTACGTGGGGGAAGGATCTGGGCTGCT
CTCCCTTGGGAACAGTGGAC

Downstream 100 bases:

>100_bases
GCGGGCCTTAAGGTTGCCTGCAATACAAACTTGTATCTCTTGCTACGAAACGAGACCAAGTTCTATGATGGGGGTAGCGA
GGCAAGATTTTCCCTGACCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MHLRDSFRRTKIVATIGPASSNPAILREMILQGATTLRLNFSHGDHELHRRSIRLIRQTAMDLGIQVAILQDLQGPKIRL
GKFAEGSITLKAGDPFVLTSKPVLGSQERSWVTYDKLAQEVPEGATILIDDGRVEMRVEAVDPEAGELFCRTIVGGTLSN
NKGVNFPGVRLSIRAVTPKDKEDLYFGLNQGVDWVALSFVRDPSDVLELRELIASAKRPSSGSADKRVPIIVKIEKHEAI
EQLPQILALSDGVMVARGDLGVELPAEEVPILQKRVIALANSLGIPVITATQMLDSMVHSPRPTRAEISDVANAILDGTD
AVMLSNETAVGKYPVEAVATMARIAVRTERDYFDLTYADRRQRLRALAMQELSQNNGNSPAGMRPKTLITDSISRAVGEI
AQELDAVAVMTLTKTGATARNVSKFRPRTPILAVTPHVEVARRLQLVWGVHPLLVMDLATTRQTFQAAISLAQEDGLLKD
GDLVVLSAGTLPGVAGSTDLIKVDVVKAVVAQGKGFGKGIVSGPARILRSSLDASKITAGDILVAQSTDANYVEAIKQAA
GVITEEEGASSHAAVIGLRLGVPVLVGVKDATRLIRDGSIVTLNPEKGIVTAGADGLGF

Sequences:

>Translated_619_residues
MHLRDSFRRTKIVATIGPASSNPAILREMILQGATTLRLNFSHGDHELHRRSIRLIRQTAMDLGIQVAILQDLQGPKIRL
GKFAEGSITLKAGDPFVLTSKPVLGSQERSWVTYDKLAQEVPEGATILIDDGRVEMRVEAVDPEAGELFCRTIVGGTLSN
NKGVNFPGVRLSIRAVTPKDKEDLYFGLNQGVDWVALSFVRDPSDVLELRELIASAKRPSSGSADKRVPIIVKIEKHEAI
EQLPQILALSDGVMVARGDLGVELPAEEVPILQKRVIALANSLGIPVITATQMLDSMVHSPRPTRAEISDVANAILDGTD
AVMLSNETAVGKYPVEAVATMARIAVRTERDYFDLTYADRRQRLRALAMQELSQNNGNSPAGMRPKTLITDSISRAVGEI
AQELDAVAVMTLTKTGATARNVSKFRPRTPILAVTPHVEVARRLQLVWGVHPLLVMDLATTRQTFQAAISLAQEDGLLKD
GDLVVLSAGTLPGVAGSTDLIKVDVVKAVVAQGKGFGKGIVSGPARILRSSLDASKITAGDILVAQSTDANYVEAIKQAA
GVITEEEGASSHAAVIGLRLGVPVLVGVKDATRLIRDGSIVTLNPEKGIVTAGADGLGF
>Mature_619_residues
MHLRDSFRRTKIVATIGPASSNPAILREMILQGATTLRLNFSHGDHELHRRSIRLIRQTAMDLGIQVAILQDLQGPKIRL
GKFAEGSITLKAGDPFVLTSKPVLGSQERSWVTYDKLAQEVPEGATILIDDGRVEMRVEAVDPEAGELFCRTIVGGTLSN
NKGVNFPGVRLSIRAVTPKDKEDLYFGLNQGVDWVALSFVRDPSDVLELRELIASAKRPSSGSADKRVPIIVKIEKHEAI
EQLPQILALSDGVMVARGDLGVELPAEEVPILQKRVIALANSLGIPVITATQMLDSMVHSPRPTRAEISDVANAILDGTD
AVMLSNETAVGKYPVEAVATMARIAVRTERDYFDLTYADRRQRLRALAMQELSQNNGNSPAGMRPKTLITDSISRAVGEI
AQELDAVAVMTLTKTGATARNVSKFRPRTPILAVTPHVEVARRLQLVWGVHPLLVMDLATTRQTFQAAISLAQEDGLLKD
GDLVVLSAGTLPGVAGSTDLIKVDVVKAVVAQGKGFGKGIVSGPARILRSSLDASKITAGDILVAQSTDANYVEAIKQAA
GVITEEEGASSHAAVIGLRLGVPVLVGVKDATRLIRDGSIVTLNPEKGIVTAGADGLGF

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=516, Percent_Identity=39.1472868217054, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI10835121, Length=516, Percent_Identity=39.1472868217054, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI33286418, Length=519, Percent_Identity=38.3429672447013, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI33286422, Length=519, Percent_Identity=38.5356454720617, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI33286420, Length=519, Percent_Identity=38.5356454720617, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI310128732, Length=300, Percent_Identity=40, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128730, Length=300, Percent_Identity=40, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128736, Length=248, Percent_Identity=36.6935483870968, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI310128734, Length=248, Percent_Identity=36.6935483870968, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI310128738, Length=227, Percent_Identity=35.2422907488987, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1787965, Length=503, Percent_Identity=42.7435387673956, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788160, Length=504, Percent_Identity=38.2936507936508, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1787994, Length=100, Percent_Identity=34, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17544584, Length=510, Percent_Identity=38.6274509803922, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17506829, Length=510, Percent_Identity=38.4313725490196, Blast_Score=291, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=510, Percent_Identity=38.4313725490196, Blast_Score=291, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=510, Percent_Identity=38.4313725490196, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=510, Percent_Identity=38.4313725490196, Blast_Score=290, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319279, Length=521, Percent_Identity=38.0038387715931, Blast_Score=296, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=506, Percent_Identity=35.1778656126482, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648964, Length=518, Percent_Identity=38.4169884169884, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI28571814, Length=518, Percent_Identity=38.4169884169884, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24648966, Length=481, Percent_Identity=36.7983367983368, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24581235, Length=511, Percent_Identity=33.8551859099804, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24646914, Length=297, Percent_Identity=40.7407407407407, Blast_Score=198, Evalue=1e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 66234; Mature: 66234

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLRDSFRRTKIVATIGPASSNPAILREMILQGATTLRLNFSHGDHELHRRSIRLIRQTA
CCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHH
MDLGIQVAILQDLQGPKIRLGKFAEGSITLKAGDPFVLTSKPVLGSQERSWVTYDKLAQE
HHCCEEEEEECCCCCCCEEECEECCCEEEEECCCCEEEECCCCCCCCCCCEEHHHHHHHH
VPEGATILIDDGRVEMRVEAVDPEAGELFCRTIVGGTLSNNKGVNFPGVRLSIRAVTPKD
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCC
KEDLYFGLNQGVDWVALSFVRDPSDVLELRELIASAKRPSSGSADKRVPIIVKIEKHEAI
CCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHH
EQLPQILALSDGVMVARGDLGVELPAEEVPILQKRVIALANSLGIPVITATQMLDSMVHS
HHHHHHHHHCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHC
PRPTRAEISDVANAILDGTDAVMLSNETAVGKYPVEAVATMARIAVRTERDYFDLTYADR
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHH
RQRLRALAMQELSQNNGNSPAGMRPKTLITDSISRAVGEIAQELDAVAVMTLTKTGATAR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHH
NVSKFRPRTPILAVTPHVEVARRLQLVWGVHPLLVMDLATTRQTFQAAISLAQEDGLLKD
HHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GDLVVLSAGTLPGVAGSTDLIKVDVVKAVVAQGKGFGKGIVSGPARILRSSLDASKITAG
CCEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEC
DILVAQSTDANYVEAIKQAAGVITEEEGASSHAAVIGLRLGVPVLVGVKDATRLIRDGSI
CEEEEECCCCHHHHHHHHHHCCEECCCCCCCCEEEEEEEECCCEEEECHHHHHHHHCCCE
VTLNPEKGIVTAGADGLGF
EEECCCCCEEEECCCCCCC
>Mature Secondary Structure
MHLRDSFRRTKIVATIGPASSNPAILREMILQGATTLRLNFSHGDHELHRRSIRLIRQTA
CCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHH
MDLGIQVAILQDLQGPKIRLGKFAEGSITLKAGDPFVLTSKPVLGSQERSWVTYDKLAQE
HHCCEEEEEECCCCCCCEEECEECCCEEEEECCCCEEEECCCCCCCCCCCEEHHHHHHHH
VPEGATILIDDGRVEMRVEAVDPEAGELFCRTIVGGTLSNNKGVNFPGVRLSIRAVTPKD
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCC
KEDLYFGLNQGVDWVALSFVRDPSDVLELRELIASAKRPSSGSADKRVPIIVKIEKHEAI
CCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHH
EQLPQILALSDGVMVARGDLGVELPAEEVPILQKRVIALANSLGIPVITATQMLDSMVHS
HHHHHHHHHCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHC
PRPTRAEISDVANAILDGTDAVMLSNETAVGKYPVEAVATMARIAVRTERDYFDLTYADR
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHH
RQRLRALAMQELSQNNGNSPAGMRPKTLITDSISRAVGEIAQELDAVAVMTLTKTGATAR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHH
NVSKFRPRTPILAVTPHVEVARRLQLVWGVHPLLVMDLATTRQTFQAAISLAQEDGLLKD
HHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GDLVVLSAGTLPGVAGSTDLIKVDVVKAVVAQGKGFGKGIVSGPARILRSSLDASKITAG
CCEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEC
DILVAQSTDANYVEAIKQAAGVITEEEGASSHAAVIGLRLGVPVLVGVKDATRLIRDGSI
CEEEEECCCCHHHHHHHHHHCCEECCCCCCCCEEEEEEEECCCEEEECHHHHHHHHCCCE
VTLNPEKGIVTAGADGLGF
EEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]