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The map label for this gene is sigA [H]

Identifier: 86608944

GI number: 86608944

Start: 1551150

End: 1552112

Strand: Direct

Name: sigA [H]

Synonym: CYB_1477

Alternate gene names: 86608944

Gene position: 1551150-1552112 (Clockwise)

Preceding gene: 86608943

Following gene: 86608947

Centisome position: 50.91

GC content: 56.49

Gene sequence:

>963_bases
ATGAAATCTCAACCAAATGGGACGAATGTAGCTGTTCAGAACTCTCCTCCTCCACTGAAGCGGGTTGAGGTACGGCTCAG
CAAACCCGGCAACCGAGCGACCCAAGCCTCGCTTTCGGAAGACTCAGTGGGCGTATTTTTGCGGGAAATGGCTCGCTACC
CACTCCTCAATGCCGAGCAGGAAGTGAAATTGGGACGGCTGATTGCAGAGGGGGGAGCCGCCGGGGAACGGGCGAAACGG
GAATTGGTTAGGGCCAACCTGCGCCTGGTGGTCTCGATTGCCAAAAAGTACTTAAATCGCGGTGTGCCGTTTCTGGATCT
GATTCAAGAAGGCACCATTGGCCTGATCCGAGCCGCAGAAAAATTTGAGTACGAACGCGGCTACAAGTTTTCCACCTACG
CCTACTGGTGGATTCGCCAAGGGATCACCCGCGCCGTTGCCAATCAGGGGCGCCTGGTGCGGATCCCGGTGCACATGGTA
GAAAAGCTCAACCAGGTGAAGCGGATCCGGCGGGAGCTGACCGAGACCCTAAACCGTCGCCCCACCCAAGCGGAAATTGC
CGAAGCTCTGGAGATTTCCCCCGACAAGCTGGACGCTCTCTTAACCCTCTTGGAAGCCGGTCGCCAAACCCTTTCGCTAC
ATACTCCAGTTGGCAAAGAGGAGGATAGGGAGCTGATGCAGCTTATCGAGGACGCCAACAGCCTCACTCCCGAATCCCAA
ATGGACTTGCACCTGCTGTCTGCCCAACTGGAAGAAATGCTGGAGCAGCTCAGCCCCCGAGAGCAAGAAATCCTCAAGCT
CCGCTATGGGCTGAAAGATGGCCACCTGCACACTCTCTCCGAAATTGGTCAGCTCTTCGGCCTGTCTCGAGAGCGCGTCC
GACAAATTCAAGCACGAGCCATGCGCAAACTGCGCCATCCACGCCGCCAGGAAATTTTGGCAGATTGGATGACCTCGTTT
TAG

Upstream 100 bases:

>100_bases
GATCCCTGAAGGAAGTTGGGTTTGCTGAAAGGTTAGCCGCCTCCATCCTGTACTGAGTAAAGTTCTTAACACTCGCTTGT
GAACTTGAGCTTGAGTTTGT

Downstream 100 bases:

>100_bases
GCAGTAGGCCACGGCTGCTCCACAGGCGAAACCCAGGATTCCGCAGCAGTTTATGTTCAGCAAAGCTCTGCAGAACCAGG
ACCAGAGGCGTGCCCCCACT

Product: sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKSQPNGTNVAVQNSPPPLKRVEVRLSKPGNRATQASLSEDSVGVFLREMARYPLLNAEQEVKLGRLIAEGGAAGERAKR
ELVRANLRLVVSIAKKYLNRGVPFLDLIQEGTIGLIRAAEKFEYERGYKFSTYAYWWIRQGITRAVANQGRLVRIPVHMV
EKLNQVKRIRRELTETLNRRPTQAEIAEALEISPDKLDALLTLLEAGRQTLSLHTPVGKEEDRELMQLIEDANSLTPESQ
MDLHLLSAQLEEMLEQLSPREQEILKLRYGLKDGHLHTLSEIGQLFGLSRERVRQIQARAMRKLRHPRRQEILADWMTSF

Sequences:

>Translated_320_residues
MKSQPNGTNVAVQNSPPPLKRVEVRLSKPGNRATQASLSEDSVGVFLREMARYPLLNAEQEVKLGRLIAEGGAAGERAKR
ELVRANLRLVVSIAKKYLNRGVPFLDLIQEGTIGLIRAAEKFEYERGYKFSTYAYWWIRQGITRAVANQGRLVRIPVHMV
EKLNQVKRIRRELTETLNRRPTQAEIAEALEISPDKLDALLTLLEAGRQTLSLHTPVGKEEDRELMQLIEDANSLTPESQ
MDLHLLSAQLEEMLEQLSPREQEILKLRYGLKDGHLHTLSEIGQLFGLSRERVRQIQARAMRKLRHPRRQEILADWMTSF
>Mature_320_residues
MKSQPNGTNVAVQNSPPPLKRVEVRLSKPGNRATQASLSEDSVGVFLREMARYPLLNAEQEVKLGRLIAEGGAAGERAKR
ELVRANLRLVVSIAKKYLNRGVPFLDLIQEGTIGLIRAAEKFEYERGYKFSTYAYWWIRQGITRAVANQGRLVRIPVHMV
EKLNQVKRIRRELTETLNRRPTQAEIAEALEISPDKLDALLTLLEAGRQTLSLHTPVGKEEDRELMQLIEDANSLTPESQ
MDLHLLSAQLEEMLEQLSPREQEILKLRYGLKDGHLHTLSEIGQLFGLSRERVRQIQARAMRKLRHPRRQEILADWMTSF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=262, Percent_Identity=47.3282442748092, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1789098, Length=295, Percent_Identity=40.6779661016949, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789871, Length=274, Percent_Identity=27.7372262773723, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36568; Mature: 36568

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQPNGTNVAVQNSPPPLKRVEVRLSKPGNRATQASLSEDSVGVFLREMARYPLLNAEQ
CCCCCCCCEEEECCCCCCHHHHEEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
EVKLGRLIAEGGAAGERAKRELVRANLRLVVSIAKKYLNRGVPFLDLIQEGTIGLIRAAE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHH
KFEYERGYKFSTYAYWWIRQGITRAVANQGRLVRIPVHMVEKLNQVKRIRRELTETLNRR
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCC
PTQAEIAEALEISPDKLDALLTLLEAGRQTLSLHTPVGKEEDRELMQLIEDANSLTPESQ
CCHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCHH
MDLHLLSAQLEEMLEQLSPREQEILKLRYGLKDGHLHTLSEIGQLFGLSRERVRQIQARA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
MRKLRHPRRQEILADWMTSF
HHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKSQPNGTNVAVQNSPPPLKRVEVRLSKPGNRATQASLSEDSVGVFLREMARYPLLNAEQ
CCCCCCCCEEEECCCCCCHHHHEEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
EVKLGRLIAEGGAAGERAKRELVRANLRLVVSIAKKYLNRGVPFLDLIQEGTIGLIRAAE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHH
KFEYERGYKFSTYAYWWIRQGITRAVANQGRLVRIPVHMVEKLNQVKRIRRELTETLNRR
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCC
PTQAEIAEALEISPDKLDALLTLLEAGRQTLSLHTPVGKEEDRELMQLIEDANSLTPESQ
CCHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCHH
MDLHLLSAQLEEMLEQLSPREQEILKLRYGLKDGHLHTLSEIGQLFGLSRERVRQIQARA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
MRKLRHPRRQEILADWMTSF
HHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]