Click here to switch to the map view.

The map label for this gene is phnD [H]

Identifier: 86608934

GI number: 86608934

Start: 1541970

End: 1542947

Strand: Direct

Name: phnD [H]

Synonym: CYB_1466

Alternate gene names: 86608934

Gene position: 1541970-1542947 (Clockwise)

Preceding gene: 86608933

Following gene: 86608935

Centisome position: 50.61

GC content: 52.86

Gene sequence:

>978_bases
ATGTTGGCGTTTTGCTCTGTGGCTCGTCTGTTATCCAAAAGCCTTGCCCTGGCTGGTTTGGTTGCTGTGCTAGTGGGTTA
TCAATCTGCTCAAGCTCAATCTTGCCCTCGTCCGGCAGGCATGGATGAGCGCTATTGTGATGCCGATGGGGATCTGGTGG
CGGATACTCCGACGGATCCCGCTCAAATTGTCAATCCTGACACTTTGCTCATCTCCTACACTTCTTCCGAGGATCCCGCC
GTTTATTCAGAAACCTGGCAGGAATTTACCGACTACCTCAAGCAAGTGACCGGTAAAGATGTGCAATTTGTGCCAGTTGA
TTCCGAGGCCGCCCAAACCGAGGCAATGCGGGCCGGACGTTTACACATCATGGGCACCTGCACCGGCTGTACCCCCCGCG
CTGTGAACCAAGCAGGCTTTGTGCCTTTCGCCGTTTTTGCCAAGGAAGATGGTAGCTTTGGCTACGAAATGGAAGTGATC
ACTCGCATCGATAGTGGCTTGGAAAAACTAGAAGACATCAAAGGCCGCCGCATTGCTTTCACCCAGCCCTCCTCCAATTC
CGGCTACATCAGCGCCCGTATTTTGATCGAGGCGGAAACTGGCTTTAAGGACGGTGTGGACTACGAAGCCACTTTCTCTG
GGGGGCACGAAAACTCCATTTTGGGTGTGTTCAACGGAGACTACGATGCGGCCCCCATTGCCAACGATGTGCTCAACCGC
ATGTGCATCGCCGGACAAGTGCAGTGCGATCAGTTTCGTACCCTCTACAAATCGGCTACTTTCCCCAGCGGCCCATTTGG
CTATGCTCACAATTTGGATCCACAGCTGCAGGAGAAAATCCGCGAGGCCTTCTATACCTTTGACTTTGCAGGCACTAAGT
TGGCAAAAGCCTTTGACCGAGCTTCCTTTGCTCCCATCACCTACAAGAAAGATTGGGAAGTGATCCGGCTGATTCAAGAA
AATGCCCCTCCCCAATAG

Upstream 100 bases:

>100_bases
AATTCACATCAAGTCTCTTGATTAGCTGGATCTTCTCGCCCACTTTTGTGTTTTTTAGCTGATAACGGTTTGCTTCATCT
TGCATTCGGGGGTTTTTGCA

Downstream 100 bases:

>100_bases
GTGGGCTGAACAGAATCGGGATCCCTTGTTCAGGGCAAGCCATAGGGGATCCCTTCTGATTTCAAAATTCTCTCAAGATT
GCAAACATCTGGTTCTAGAA

Product: phosphonate ABC transporter, periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLAFCSVARLLSKSLALAGLVAVLVGYQSAQAQSCPRPAGMDERYCDADGDLVADTPTDPAQIVNPDTLLISYTSSEDPA
VYSETWQEFTDYLKQVTGKDVQFVPVDSEAAQTEAMRAGRLHIMGTCTGCTPRAVNQAGFVPFAVFAKEDGSFGYEMEVI
TRIDSGLEKLEDIKGRRIAFTQPSSNSGYISARILIEAETGFKDGVDYEATFSGGHENSILGVFNGDYDAAPIANDVLNR
MCIAGQVQCDQFRTLYKSATFPSGPFGYAHNLDPQLQEKIREAFYTFDFAGTKLAKAFDRASFAPITYKKDWEVIRLIQE
NAPPQ

Sequences:

>Translated_325_residues
MLAFCSVARLLSKSLALAGLVAVLVGYQSAQAQSCPRPAGMDERYCDADGDLVADTPTDPAQIVNPDTLLISYTSSEDPA
VYSETWQEFTDYLKQVTGKDVQFVPVDSEAAQTEAMRAGRLHIMGTCTGCTPRAVNQAGFVPFAVFAKEDGSFGYEMEVI
TRIDSGLEKLEDIKGRRIAFTQPSSNSGYISARILIEAETGFKDGVDYEATFSGGHENSILGVFNGDYDAAPIANDVLNR
MCIAGQVQCDQFRTLYKSATFPSGPFGYAHNLDPQLQEKIREAFYTFDFAGTKLAKAFDRASFAPITYKKDWEVIRLIQE
NAPPQ
>Mature_325_residues
MLAFCSVARLLSKSLALAGLVAVLVGYQSAQAQSCPRPAGMDERYCDADGDLVADTPTDPAQIVNPDTLLISYTSSEDPA
VYSETWQEFTDYLKQVTGKDVQFVPVDSEAAQTEAMRAGRLHIMGTCTGCTPRAVNQAGFVPFAVFAKEDGSFGYEMEVI
TRIDSGLEKLEDIKGRRIAFTQPSSNSGYISARILIEAETGFKDGVDYEATFSGGHENSILGVFNGDYDAAPIANDVLNR
MCIAGQVQCDQFRTLYKSATFPSGPFGYAHNLDPQLQEKIREAFYTFDFAGTKLAKAFDRASFAPITYKKDWEVIRLIQE
NAPPQ

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=250, Percent_Identity=24, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35555; Mature: 35555

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFCSVARLLSKSLALAGLVAVLVGYQSAQAQSCPRPAGMDERYCDADGDLVADTPTDP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCH
AQIVNPDTLLISYTSSEDPAVYSETWQEFTDYLKQVTGKDVQFVPVDSEAAQTEAMRAGR
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
LHIMGTCTGCTPRAVNQAGFVPFAVFAKEDGSFGYEMEVITRIDSGLEKLEDIKGRRIAF
EEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEE
TQPSSNSGYISARILIEAETGFKDGVDYEATFSGGHENSILGVFNGDYDAAPIANDVLNR
ECCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
MCIAGQVQCDQFRTLYKSATFPSGPFGYAHNLDPQLQEKIREAFYTFDFAGTKLAKAFDR
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASFAPITYKKDWEVIRLIQENAPPQ
CCCCCEEECCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAFCSVARLLSKSLALAGLVAVLVGYQSAQAQSCPRPAGMDERYCDADGDLVADTPTDP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCH
AQIVNPDTLLISYTSSEDPAVYSETWQEFTDYLKQVTGKDVQFVPVDSEAAQTEAMRAGR
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
LHIMGTCTGCTPRAVNQAGFVPFAVFAKEDGSFGYEMEVITRIDSGLEKLEDIKGRRIAF
EEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEE
TQPSSNSGYISARILIEAETGFKDGVDYEATFSGGHENSILGVFNGDYDAAPIANDVLNR
ECCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
MCIAGQVQCDQFRTLYKSATFPSGPFGYAHNLDPQLQEKIREAFYTFDFAGTKLAKAFDR
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASFAPITYKKDWEVIRLIQENAPPQ
CCCCCEEECCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]