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The map label for this gene is phnD-2 [H]

Identifier: 86608933

GI number: 86608933

Start: 1540613

End: 1541620

Strand: Direct

Name: phnD-2 [H]

Synonym: CYB_1465

Alternate gene names: 86608933

Gene position: 1540613-1541620 (Clockwise)

Preceding gene: 86608932

Following gene: 86608934

Centisome position: 50.57

GC content: 52.58

Gene sequence:

>1008_bases
ATGATGACACTATCTTCTTTTGGAACTCGGATCCCGTTGGGAAAGCTGGTGATGGGAGCTGGATTGGCGGGAGCTGTCAC
CTTGGGGTTGGGCAGCAGCCTTTCTCAGGCTCGTCTAGACTGTCCCCGCCCTCCTCAAATGGATGCCCGCTACTGTGATC
GGGTGGGGGATCTGGTTGCCGATTTGCCGGAGGATCCCTCGGAGTGGGTAGATCCGGAAGTGCTGTTGGTGTCTTATACC
TCCTCAGAAGAGCCAGCTGTCTATGCCAATACCTGGGCTGAATTCACCGCTCACTTAGAGGAAGTCACCGGTAGACGGGT
GCAATTTGTCCCTGCCGATTCTCAGGCTGCTCAGTACGAGGCAATGCGCGCTGGACGTCTACACATTATGGGTACCTGCA
CAGGCTGTACTCCTGTAGCCGTGAATGTTGCCGGCTTTGTGCCCTTTGCCATTTTCCAACAAGAAGATGGATCCATCGGC
TACGAGATGGAAGTGATCACTCATGTGAATAGCGATATTCAAGCCCTCGATGATATTGCCGGTCGTCGTGTAGCTTTTAC
CCATCCTTCCTCCAACTCCGGTTATATCAGTGCTCGCATTTTGATCAAACAAGAGACAGGCTTGGATGATGAGGCAGGAG
ACTACATCGCCGAGTTTTCTGGCAGCCACCAAAACTCCATTTTGGGTGTGTTCAACGAAGACTACGATGCCGCCCCCATT
GCCAACGATGTGCTTAATCGCATGTGCCTCAACGAGCAAGTGGATTGTAGCCAGTTCCGCAGCCTCTACAAATCGGAGAC
CTTCCCCAGCGGCTCTTTCGGCCATGCCCACAATCTCCACCCTGAGCTGGCAGAAAGGATCCGAGAAGCCTTCTTTACTT
TTGAGTTTGAGGGCACAGCCATGCGACAAGCTTTTGACCGGGTAGCTTTTGCACCGATTACTTATCAAGAAAACTGGGAG
ATCATCCGTCGCATTCAGGAAGAAGAGGGCGTGGAATATACGCTTTAG

Upstream 100 bases:

>100_bases
GCTTTTCAGGCAAGACTTCTAGGGCCTAGAGGAGGAATTTTTACCTCTTCTGGGTTCGTCAGATGCTCTTGTTGATTGCC
ATAGTTGGGAGCTTTTATTC

Downstream 100 bases:

>100_bases
GCAGTCCTCAGGGATCCCCTGCCCAGAGAGGATCCCTGATTTACATAGTTCTGAATCTAGAGCTGAATCTAGAGAAATCG
CACTGGCTGGTTCCGTCCAT

Product: phosphonate ABC transporter, periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MMTLSSFGTRIPLGKLVMGAGLAGAVTLGLGSSLSQARLDCPRPPQMDARYCDRVGDLVADLPEDPSEWVDPEVLLVSYT
SSEEPAVYANTWAEFTAHLEEVTGRRVQFVPADSQAAQYEAMRAGRLHIMGTCTGCTPVAVNVAGFVPFAIFQQEDGSIG
YEMEVITHVNSDIQALDDIAGRRVAFTHPSSNSGYISARILIKQETGLDDEAGDYIAEFSGSHQNSILGVFNEDYDAAPI
ANDVLNRMCLNEQVDCSQFRSLYKSETFPSGSFGHAHNLHPELAERIREAFFTFEFEGTAMRQAFDRVAFAPITYQENWE
IIRRIQEEEGVEYTL

Sequences:

>Translated_335_residues
MMTLSSFGTRIPLGKLVMGAGLAGAVTLGLGSSLSQARLDCPRPPQMDARYCDRVGDLVADLPEDPSEWVDPEVLLVSYT
SSEEPAVYANTWAEFTAHLEEVTGRRVQFVPADSQAAQYEAMRAGRLHIMGTCTGCTPVAVNVAGFVPFAIFQQEDGSIG
YEMEVITHVNSDIQALDDIAGRRVAFTHPSSNSGYISARILIKQETGLDDEAGDYIAEFSGSHQNSILGVFNEDYDAAPI
ANDVLNRMCLNEQVDCSQFRSLYKSETFPSGSFGHAHNLHPELAERIREAFFTFEFEGTAMRQAFDRVAFAPITYQENWE
IIRRIQEEEGVEYTL
>Mature_335_residues
MMTLSSFGTRIPLGKLVMGAGLAGAVTLGLGSSLSQARLDCPRPPQMDARYCDRVGDLVADLPEDPSEWVDPEVLLVSYT
SSEEPAVYANTWAEFTAHLEEVTGRRVQFVPADSQAAQYEAMRAGRLHIMGTCTGCTPVAVNVAGFVPFAIFQQEDGSIG
YEMEVITHVNSDIQALDDIAGRRVAFTHPSSNSGYISARILIKQETGLDDEAGDYIAEFSGSHQNSILGVFNEDYDAAPI
ANDVLNRMCLNEQVDCSQFRSLYKSETFPSGSFGHAHNLHPELAERIREAFFTFEFEGTAMRQAFDRVAFAPITYQENWE
IIRRIQEEEGVEYTL

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 36989; Mature: 36989

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLSSFGTRIPLGKLVMGAGLAGAVTLGLGSSLSQARLDCPRPPQMDARYCDRVGDLVA
CCCCHHCCCCCCHHHHHHCCCCHHHHEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
DLPEDPSEWVDPEVLLVSYTSSEEPAVYANTWAEFTAHLEEVTGRRVQFVPADSQAAQYE
CCCCCCHHHCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
AMRAGRLHIMGTCTGCTPVAVNVAGFVPFAIFQQEDGSIGYEMEVITHVNSDIQALDDIA
HHHCCCEEEEEEECCCCCEEEEHHHHCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHC
GRRVAFTHPSSNSGYISARILIKQETGLDDEAGDYIAEFSGSHQNSILGVFNEDYDAAPI
CCEEEEECCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCH
ANDVLNRMCLNEQVDCSQFRSLYKSETFPSGSFGHAHNLHPELAERIREAFFTFEFEGTA
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCHH
MRQAFDRVAFAPITYQENWEIIRRIQEEEGVEYTL
HHHHHHHHHCCEEEECCCHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MMTLSSFGTRIPLGKLVMGAGLAGAVTLGLGSSLSQARLDCPRPPQMDARYCDRVGDLVA
CCCCHHCCCCCCHHHHHHCCCCHHHHEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
DLPEDPSEWVDPEVLLVSYTSSEEPAVYANTWAEFTAHLEEVTGRRVQFVPADSQAAQYE
CCCCCCHHHCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
AMRAGRLHIMGTCTGCTPVAVNVAGFVPFAIFQQEDGSIGYEMEVITHVNSDIQALDDIA
HHHCCCEEEEEEECCCCCEEEEHHHHCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHC
GRRVAFTHPSSNSGYISARILIKQETGLDDEAGDYIAEFSGSHQNSILGVFNEDYDAAPI
CCEEEEECCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCH
ANDVLNRMCLNEQVDCSQFRSLYKSETFPSGSFGHAHNLHPELAERIREAFFTFEFEGTA
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCHH
MRQAFDRVAFAPITYQENWEIIRRIQEEEGVEYTL
HHHHHHHHHCCEEEECCCHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]