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The map label for this gene is aspB

Identifier: 86608889

GI number: 86608889

Start: 1489481

End: 1490731

Strand: Reverse

Name: aspB

Synonym: CYB_1421

Alternate gene names: 86608889

Gene position: 1490731-1489481 (Counterclockwise)

Preceding gene: 86608891

Following gene: 86608887

Centisome position: 48.93

GC content: 60.67

Gene sequence:

>1251_bases
ATGGCCAGGATCAACGACCACTTCCTCAAGCTCAAGACCGGCTATCTCTTTCCCGAGATCGCCCGGCGGGTGCAGGCTTT
TGCCACCGCCCATCCCGAAGCTCAAATTATCAAAATGGGCATTGGCGATGTCACCGAGCCGCTGCCGGAAGCCTGTCGAA
CGGCCATGATCCGGGCGGTGGAGGAAATGGGGGAACGGGCCACCTTTCGCGGCTATGGGCCAGAGCAGGGCTACGAGTGG
CTGCGCCAGGCCATTGCCCGGCACGACTTCCAGGCCCGCAACTGCGATATCGACGCTTCCGAGATCTTCGTCTCCGATGG
CTCCAAGTGCGATTGCGGCAACATCCTGGATATCCTCGGCCACGACAACACCATCGCCATCACGGATCCTGTCTATCCGG
TTTATGTGGACACCAACGTGATGGCGGGCCATACGGGGCCGGCCAACGAACGCGGCGAATACGAGGGCCTGGTCTATCTG
CCCATCACTGCCGAAAATCACTTCACCGCCAGCCTGCCCAGCCAAAAGGTGGATGTGATCTACCTCTGCTTCCCCAATAA
CCCCACAGGCGCAGTGGCCACACGGGAGCATCTGCAAAACTGGGTGGACTATGCCCGTGCCCACGGCTCCCTGATCCTGT
TCGATGCCGCCTACGAAGCCTATATCACCGAGCCCGGGATCCCCCACTCCATTTACGAGATTCCAGGGGCGCGGGAATGT
GCCATCGAGTTCCGCTCCTTTTCCAAAACCGCTGGGTTTACCGGCACCCGCTGTGCCTTTACGGTGGTGCCCAAGTCTTT
GCGGGGGCAAGCTGCCGATGGATCCTGGGTGGATCTGTGGAGCTTGTGGTATCGCCGTCAATCCACCAAGTTCAACGGGG
TGTCCTACATTGTGCAGCGGGGGGCGGAGGCGGTCTATTCCGAAGCGGGGCAAGCGCAGGTGCAAGGTCTGGTGCAGTTT
TACCTGGAAAATGCCCGCATCATTCGCCAGCAGTTGGCCGAAGTGGGGATCCAAGTCTATGGCGGCGTGAATGCCCCCTA
CGTCTGGGTGAAAACACCCGATGGGCTGAGCAGTTGGGAGTTTTTCGACAAGCTGCTGCACACCTGCCATGTGGTGGGTA
CCCCCGGCTCTGGCTTCGGCAGCGCCGGAGAGGGCTATCTGCGCCTGTCCGCTTTCAACAGCCGCGCCAACGTGGAAGAG
GCAATGCGCCGCATTGGCAGCGTGTTTGCGGGGGCTGGAGCCGTTGGTTAA

Upstream 100 bases:

>100_bases
GACGTAGAAGAGTTCTGGCCCGGAACCGGCTAAGGACTCCGTCCCGCTAGCGCGAGCAGCTACACTGAAAAGCCGATGAC
CTCCTCTTCGGCAAACCTCC

Downstream 100 bases:

>100_bases
TTGAGTCTTGACCCAGAGGGCGAGGATTCTTTCAAGCGCTCCCCGCCGCCAGAGGCGAACAGCTTGGGCCCGACAGGAAA
GATGACGAACCAGTCGGCGA

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MARINDHFLKLKTGYLFPEIARRVQAFATAHPEAQIIKMGIGDVTEPLPEACRTAMIRAVEEMGERATFRGYGPEQGYEW
LRQAIARHDFQARNCDIDASEIFVSDGSKCDCGNILDILGHDNTIAITDPVYPVYVDTNVMAGHTGPANERGEYEGLVYL
PITAENHFTASLPSQKVDVIYLCFPNNPTGAVATREHLQNWVDYARAHGSLILFDAAYEAYITEPGIPHSIYEIPGAREC
AIEFRSFSKTAGFTGTRCAFTVVPKSLRGQAADGSWVDLWSLWYRRQSTKFNGVSYIVQRGAEAVYSEAGQAQVQGLVQF
YLENARIIRQQLAEVGIQVYGGVNAPYVWVKTPDGLSSWEFFDKLLHTCHVVGTPGSGFGSAGEGYLRLSAFNSRANVEE
AMRRIGSVFAGAGAVG

Sequences:

>Translated_416_residues
MARINDHFLKLKTGYLFPEIARRVQAFATAHPEAQIIKMGIGDVTEPLPEACRTAMIRAVEEMGERATFRGYGPEQGYEW
LRQAIARHDFQARNCDIDASEIFVSDGSKCDCGNILDILGHDNTIAITDPVYPVYVDTNVMAGHTGPANERGEYEGLVYL
PITAENHFTASLPSQKVDVIYLCFPNNPTGAVATREHLQNWVDYARAHGSLILFDAAYEAYITEPGIPHSIYEIPGAREC
AIEFRSFSKTAGFTGTRCAFTVVPKSLRGQAADGSWVDLWSLWYRRQSTKFNGVSYIVQRGAEAVYSEAGQAQVQGLVQF
YLENARIIRQQLAEVGIQVYGGVNAPYVWVKTPDGLSSWEFFDKLLHTCHVVGTPGSGFGSAGEGYLRLSAFNSRANVEE
AMRRIGSVFAGAGAVG
>Mature_415_residues
ARINDHFLKLKTGYLFPEIARRVQAFATAHPEAQIIKMGIGDVTEPLPEACRTAMIRAVEEMGERATFRGYGPEQGYEWL
RQAIARHDFQARNCDIDASEIFVSDGSKCDCGNILDILGHDNTIAITDPVYPVYVDTNVMAGHTGPANERGEYEGLVYLP
ITAENHFTASLPSQKVDVIYLCFPNNPTGAVATREHLQNWVDYARAHGSLILFDAAYEAYITEPGIPHSIYEIPGARECA
IEFRSFSKTAGFTGTRCAFTVVPKSLRGQAADGSWVDLWSLWYRRQSTKFNGVSYIVQRGAEAVYSEAGQAQVQGLVQFY
LENARIIRQQLAEVGIQVYGGVNAPYVWVKTPDGLSSWEFFDKLLHTCHVVGTPGSGFGSAGEGYLRLSAFNSRANVEEA
MRRIGSVFAGAGAVG

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=356, Percent_Identity=28.9325842696629, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_SYNJB (Q2JLL9)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477651.1
- ProteinModelPortal:   Q2JLL9
- SMR:   Q2JLL9
- STRING:   Q2JLL9
- GeneID:   3901325
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1421
- TIGR:   CYB_1421
- eggNOG:   COG0436
- HOGENOM:   HBG645860
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- BioCyc:   SSP321332:CYB_1421-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 45787; Mature: 45656

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 187-187 BINDING 187-187 BINDING 215-215 BINDING 218-218 BINDING 246-246 BINDING 248-248 BINDING 257-257 BINDING 292-292 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARINDHFLKLKTGYLFPEIARRVQAFATAHPEAQIIKMGIGDVTEPLPEACRTAMIRAV
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
EEMGERATFRGYGPEQGYEWLRQAIARHDFQARNCDIDASEIFVSDGSKCDCGNILDILG
HHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCCHHHHHC
HDNTIAITDPVYPVYVDTNVMAGHTGPANERGEYEGLVYLPITAENHFTASLPSQKVDVI
CCCEEEEECCCEEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEE
YLCFPNNPTGAVATREHLQNWVDYARAHGSLILFDAAYEAYITEPGIPHSIYEIPGAREC
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHCCCCCHHH
AIEFRSFSKTAGFTGTRCAFTVVPKSLRGQAADGSWVDLWSLWYRRQSTKFNGVSYIVQR
HHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GAEAVYSEAGQAQVQGLVQFYLENARIIRQQLAEVGIQVYGGVNAPYVWVKTPDGLSSWE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHH
FFDKLLHTCHVVGTPGSGFGSAGEGYLRLSAFNSRANVEEAMRRIGSVFAGAGAVG
HHHHHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARINDHFLKLKTGYLFPEIARRVQAFATAHPEAQIIKMGIGDVTEPLPEACRTAMIRAV
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
EEMGERATFRGYGPEQGYEWLRQAIARHDFQARNCDIDASEIFVSDGSKCDCGNILDILG
HHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCCHHHHHC
HDNTIAITDPVYPVYVDTNVMAGHTGPANERGEYEGLVYLPITAENHFTASLPSQKVDVI
CCCEEEEECCCEEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEE
YLCFPNNPTGAVATREHLQNWVDYARAHGSLILFDAAYEAYITEPGIPHSIYEIPGAREC
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHCCCCCHHH
AIEFRSFSKTAGFTGTRCAFTVVPKSLRGQAADGSWVDLWSLWYRRQSTKFNGVSYIVQR
HHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GAEAVYSEAGQAQVQGLVQFYLENARIIRQQLAEVGIQVYGGVNAPYVWVKTPDGLSSWE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHH
FFDKLLHTCHVVGTPGSGFGSAGEGYLRLSAFNSRANVEEAMRRIGSVFAGAGAVG
HHHHHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA